Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 702,939 T→C T225A (ACC→GCC)  nagB ← glucosamine‑6‑phosphate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913702,9390TC98.2% 396.6 / ‑4.6 109T225A (ACC→GCC) nagBglucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (55/51);  total (57/52)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAG  >  NC_000913/702842‑703035
                                                                                                 |                                                                                                
tCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGccc                                                                                                <  1:1180647/100‑1 (MQ=255)
tCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGccc                                                                                                <  2:2979953/100‑1 (MQ=255)
 cATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCca                                                                                               >  1:1216343/1‑100 (MQ=255)
 cATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCca                                                                                               >  2:490689/1‑100 (MQ=255)
   ttGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCcaca                                                                                             <  2:834526/100‑1 (MQ=255)
     gAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACata                                                                                           >  2:2912860/1‑100 (MQ=255)
     gAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACata                                                                                           >  1:234744/1‑100 (MQ=255)
       aaTATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATg                                                                                         <  2:784674/100‑1 (MQ=255)
       aaTATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATg                                                                                         >  1:2931703/1‑100 (MQ=255)
       aaTATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATg                                                                                         >  1:2283563/1‑100 (MQ=255)
         tatCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGt                                                                                       <  1:1921227/100‑1 (MQ=255)
           tCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTc                                                                                     >  2:844528/1‑100 (MQ=255)
           tCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTc                                                                                     <  2:1205675/100‑1 (MQ=255)
            cTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCa                                                                                    >  1:979657/1‑100 (MQ=255)
            cTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCa                                                                                    >  1:504660/1‑100 (MQ=255)
            cTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCa                                                                                    >  1:1695685/1‑100 (MQ=255)
             ttAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCAc                                                                                   >  2:902102/1‑100 (MQ=255)
             ttAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTCCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCAc                                                                                   >  2:2103394/1‑100 (MQ=255)
              tAAAGTCTTACCTTTCAGCTGCATGGTGGAAGTTTCATCCCACACCGTCATCGCTTTGGGATACAGTTGCAGACAGCTAACGGTCCACCTATGGTTAACg                                                                                  >  2:977652/1‑100 (MQ=255)
              tAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACg                                                                                  >  2:2570535/1‑100 (MQ=255)
              tAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACg                                                                                  >  2:1312921/1‑100 (MQ=255)
               aaaGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGc                                                                                 >  2:1072101/1‑100 (MQ=255)
                 aGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCaa                                                                               <  2:887925/100‑1 (MQ=255)
                 aGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCaa                                                                               >  2:1424536/1‑100 (MQ=255)
                   tCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAAcc                                                                             <  1:2502529/100‑1 (MQ=255)
                    cTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCt                                                                            >  1:479357/1‑100 (MQ=255)
                    cTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCt                                                                            >  1:2826268/1‑100 (MQ=255)
                      tAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTc                                                                          >  2:1413091/1‑100 (MQ=255)
                      tAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTc                                                                          >  2:1861879/1‑100 (MQ=255)
                          tttCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAAcg                                                                      <  1:786649/100‑1 (MQ=255)
                           ttCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAAcgg                                                                     <  1:397929/100‑1 (MQ=255)
                            tCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAAcggc                                                                    >  1:1856424/1‑100 (MQ=255)
                               gCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAATggcggc                                                                 >  1:374282/1‑100 (MQ=255)
                               gCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACggcggc                                                                 >  1:2627254/1‑100 (MQ=255)
                                cTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGcc                                                                >  2:2762103/1‑100 (MQ=255)
                                  ccATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTg                                                              <  1:2617198/100‑1 (MQ=255)
                                  ccATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTg                                                              <  2:2187554/100‑1 (MQ=255)
                                  ccATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTg                                                              >  2:198758/1‑100 (MQ=255)
                                  ccATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTg                                                              >  2:123970/1‑100 (MQ=255)
                                      ggtggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAgc                                                          <  1:1501474/100‑1 (MQ=255)
                                      ggtggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAgc                                                          >  1:1615779/1‑100 (MQ=255)
                                       gtggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAgcg                                                         >  2:2960115/1‑100 (MQ=255)
                                       gtggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAgcg                                                         <  1:1424544/100‑1 (MQ=255)
                                       gtggAAGGTCCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAgcg                                                         <  1:652558/100‑1 (MQ=255)
                                         ggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCTcc                                                       <  2:243445/100‑1 (MQ=255)
                                         ggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGcc                                                       <  2:1315954/100‑1 (MQ=255)
                                         ggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGcc                                                       >  1:2764318/1‑100 (MQ=255)
                                         ggAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGcc                                                       <  1:2560391/100‑1 (MQ=255)
                                          gAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCa                                                      <  1:1785910/100‑1 (MQ=255)
                                           aaGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAg                                                     <  1:1950940/100‑1 (MQ=255)
                                            aGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGt                                                    <  2:1501604/100‑1 (MQ=255)
                                             ggTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTg                                                   <  1:1242495/100‑1 (MQ=255)
                                             ggTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTg                                                   <  2:1615770/100‑1 (MQ=255)
                                             ggTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTg                                                   >  1:1589681/1‑100 (MQ=255)
                                              gTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGc                                                  >  2:1674304/1‑100 (MQ=255)
                                                 cATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCttt                                               >  2:2727457/1‑100 (MQ=255)
                                                  aTCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCGAGTGCTTTc                                              >  2:2767846/1‑100 (MQ=255)
                                                   tCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCt                                             <  2:907802/100‑1 (MQ=255)
                                                   tCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCt                                             <  2:926161/100‑1 (MQ=255)
                                                    cGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTg                                            <  1:299714/100‑1 (MQ=255)
                                                    cGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTg                                            <  2:96236/100‑1 (MQ=255)
                                                     gCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTgg                                           <  2:1308197/100‑1 (MQ=255)
                                                     gCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTgg                                           <  1:1290299/100‑1 (MQ=255)
                                                     gCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTgg                                           >  1:2165522/1‑100 (MQ=255)
                                                     gCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTgg                                           <  2:1549543/100‑1 (MQ=255)
                                                     gCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTgg                                           <  1:844531/100‑1 (MQ=255)
                                                     gCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTgg                                           >  1:673055/1‑100 (MQ=255)
                                                      cacaCCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGGCCACGTATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGc                                          <  1:1413099/100‑1 (MQ=255)
                                                      cacaCCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGc                                          <  1:1228152/100‑1 (MQ=255)
                                                        cacCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTa                                        >  1:575215/1‑100 (MQ=255)
                                                        cacCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTa                                        >  1:2863221/1‑100 (MQ=255)
                                                          ccATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTAcc                                      >  2:720018/1‑100 (MQ=255)
                                                          ccATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTAcc                                      >  1:1380661/1‑100 (MQ=255)
                                                           cATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTAccc                                     <  2:1363683/100‑1 (MQ=255)
                                                             tGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAg                                   <  1:2139388/100‑1 (MQ=255)
                                                              gATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGc                                  >  1:2288093/1‑100 (MQ=255)
                                                              gATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGc                                  <  2:2863205/100‑1 (MQ=255)
                                                               aTCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCa                                 <  1:1981979/100‑1 (MQ=255)
                                                                tCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCCACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCAc                                >  1:460716/1‑100 (MQ=255)
                                                                tCGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCAc                                >  1:2541798/1‑100 (MQ=255)
                                                                 cGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCAcc                               <  1:1729029/100‑1 (MQ=255)
                                                                 cGCTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCAcc                               >  1:731792/1‑100 (MQ=255)
                                                                   cTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCATCGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAg                             >  2:2954424/1‑100 (MQ=255)
                                                                   cTTTCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAg                             <  1:2570549/100‑1 (MQ=255)
                                                                      tCGGATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAat                          >  1:2792572/1‑100 (MQ=255)
                                                                        ggATGCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAatca                        >  2:528489/1‑100 (MQ=255)
                                                                            gCAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCAc                    <  2:1216337/100‑1 (MQ=255)
                                                                             cAGTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCAct                   <  2:2283551/100‑1 (MQ=255)
                                                                              aGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCActt                  >  2:1309075/1‑100 (MQ=255)
                                                                               gTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCActtc                 >  2:59512/1‑100 (MQ=255)
                                                                               gTTGCAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCActtc                 >  1:1581276/1‑100 (MQ=255)
                                                                                   cAGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCg             <  1:2041932/100‑1 (MQ=255)
                                                                                    aGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCgg            <  1:1845163/100‑1 (MQ=255)
                                                                                    aGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCgg            <  1:694077/100‑1 (MQ=255)
                                                                                    aGACAGCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTCTCTGGCTACCCAGCACCAGAATCATCACTTCTTCgg            <  1:2103406/100‑1 (MQ=255)
                                                                                         gCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATcc       <  1:1368951/100‑1 (MQ=255)
                                                                                         gCTGATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCACCACTTCTTCGGCATcc       >  1:95205/1‑100 (MQ=255)
                                                                                            gATGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagc    <  1:1072106/100‑1 (MQ=255)
                                                                                             aTGGCCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagca   <  1:2732750/100‑1 (MQ=255)
                                                                                              tGGCCCACATATGGTTCACGAAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCcagcag  >  2:2713994/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TCATTGAAATATCTTAAAGTCTTAACTTTCAGCTCCATGGTGGAAGGTTCATCGCACACCATGATCGCTTTCGGATGCAGTTGCAGACAGCTGATGGTCCACATATGGTTCACGCAACCTTCAACGGCGGCCTGCAGCGCCAGTGCTTTCTGGCTACCCAGCACCAGAATCATCACTTCTTCGGCATCCAGCAG  >  NC_000913/702842‑703035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: