Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,859 Δ1 bp 16.3% intergenic (+17/‑96) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8590C.16.3% 292.2 / 57.2 104intergenic (+17/‑96)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base C (43/43);  new base . (7/10);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 108 total aligned reads displayed.

CCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATG  >  NC_000913/1868761‑1868957
                                                                                                  |                                                                                                   
ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTaca                                                                                                    >  1:1929843/1‑100 (MQ=255)
ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTaca                                                                                                    <  2:1854883/100‑1 (MQ=255)
   tATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAt                                                                                                 >  2:103855/1‑100 (MQ=255)
     tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGt                                                                                               >  2:526283/1‑100 (MQ=255)
      gatgGAAAAAGGCTACACCCGTAACCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGt                                                                                               <  1:1320707/99‑1 (MQ=255)
      gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGtt                                                                                             >  2:436378/1‑100 (MQ=255)
      gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGtgt                                                                                             >  2:2053259/1‑98 (MQ=255)
         ggAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTAcc                                                                                          <  1:2202873/100‑1 (MQ=255)
          gAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCg                                                                                         >  1:851795/1‑100 (MQ=255)
           aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCgg                                                                                        >  2:2099265/1‑100 (MQ=255)
            aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                       <  2:1250985/100‑1 (MQ=255)
            aaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                       <  1:2053252/100‑1 (MQ=255)
             aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                      <  1:1470029/100‑1 (MQ=255)
             aaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGc                                                                                      <  2:1277599/100‑1 (MQ=255)
              aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACTTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                     <  2:2339223/100‑1 (MQ=255)
              aaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcc                                                                                     <  1:1512126/100‑1 (MQ=255)
               aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                    <  2:281412/100‑2 (MQ=39)
               aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGcca                                                                                    <  1:1885485/100‑2 (MQ=39)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTa                                                                                   >  2:1615786/1‑100 (MQ=255)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCgggtcag                                                                                   <  2:851797/100‑5 (MQ=255)
                ggCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTCGCCCGGTAACATGTGTACCGGGccag                                                                                   <  2:1771098/100‑3 (MQ=38)
                  cTACCCCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAGATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACTGGGccaggg                                                                                 <  1:1247505/100‑5 (MQ=37)
                  cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACa                                                                                 >  2:2685498/1‑100 (MQ=255)
                    acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTACTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATCTTACCGGGCCTACAAc                                                                               >  1:691138/1‑100 (MQ=255)
                       cccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagggt                                                                                <  1:2605371/96‑6 (MQ=38)
                        ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACGCTGGCCCGGTACACATGTTACCGTGCCTACAACGACa                                                                           <  1:1552215/100‑1 (MQ=255)
                        ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACa                                                                           <  1:945461/100‑1 (MQ=255)
                           tAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCg                                                                        <  2:2358928/100‑1 (MQ=255)
                            aaaCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGa                                                                       <  2:2688802/100‑1 (MQ=255)
                             aaCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGaa                                                                      >  2:397958/1‑100 (MQ=255)
                                 ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACCGCGAACCGt                                                                  >  1:909627/1‑100 (MQ=255)
                                   tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTgg                                                                >  1:2783490/1‑100 (MQ=255)
                                     cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                              >  1:56390/1‑100 (MQ=255)
                                     cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                              <  1:1041285/100‑1 (MQ=255)
                                     cGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                              >  1:2431113/1‑100 (MQ=255)
                                      gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGcc                                                             >  1:1842533/1‑100 (MQ=255)
                                      gTTTACGGTGCTAATGGTATTTGCGCGTACGTAAATCGTCTTAACAGCCCTGGCCCGGTACACATGTTAGCGGGCCTATAACGACAGCGAACCGTGGGcc                                                             <  1:146986/100‑1 (MQ=255)
                                             gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                      >  2:636475/1‑100 (MQ=255)
                                             gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                      >  2:822736/1‑100 (MQ=255)
                                             gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                      >  1:777784/1‑100 (MQ=255)
                                               gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                    <  1:1789387/100‑1 (MQ=255)
                                               gCGAATGGTATTTGCGCGTACATAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGCAg                                                      >  2:2810471/1‑98 (MQ=255)
                                                 gAATGGTATTTGCGCGTACGTAAATCGGCTTAACAACCCGGGGCCGGTACGCCTGTTACCGGGCCTACAACGACAGCGCACCGTGGGCCTGAGAAGCGGc                                                  <  1:1079209/100‑1 (MQ=255)
                                                 gAATGATATCTGCGCGTACGTAAATCGTCGTAACAACCCGGGCCCGGTACACATGTTACCGGGCCTACAACGACACCGAACCGTGGGCCTGAGAAGCGGc                                                  <  1:2033305/100‑1 (MQ=255)
                                                  aaTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                 <  1:2342068/100‑1 (MQ=255)
                                                   aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACCGCGAACCGTGGGCCTGAGAAGCGGCaa                                                >  1:491132/1‑100 (MQ=255)
                                                   aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa                                                <  1:2826671/100‑1 (MQ=255)
                                                    tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac                                               <  1:1739199/100‑1 (MQ=255)
                                                    tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAac                                               <  1:1625099/100‑1 (MQ=255)
                                                        aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                           <  2:2783500/100‑1 (MQ=255)
                                                        aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg                                           >  2:1111955/1‑100 (MQ=255)
                                                         tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                          <  1:1639454/100‑1 (MQ=255)
                                                         tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                          <  2:287533/100‑1 (MQ=255)
                                                           tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                        >  2:1335217/1‑100 (MQ=255)
                                                           tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                        <  2:1279850/100‑1 (MQ=255)
                                                           tGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCg                                        <  1:2792424/100‑1 (MQ=255)
                                                             cgcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa                                      >  2:688762/1‑100 (MQ=255)
                                                             cgcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTa                                      >  1:958294/1‑100 (MQ=255)
                                                              gcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTCAGAAGCGGCAACACAGGCGTAg                                     <  1:1690070/100‑1 (MQ=255)
                                                               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACACCGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAg                                     <  1:906327/99‑1 (MQ=255)
                                                               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                    >  1:892956/1‑100 (MQ=255)
                                                               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                    <  2:1940275/100‑1 (MQ=255)
                                                               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                    <  2:319879/100‑1 (MQ=255)
                                                               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGc                                    <  1:999712/100‑1 (MQ=255)
                                                                gtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCa                                   <  2:1211776/100‑1 (MQ=255)
                                                                  acgtaAATCGTCTTAACAACCCTGGCCCGGTAGACATGTTACCGGGCCAACAACGACAGCGACCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATa                                 >  1:2683972/1‑100 (MQ=255)
                                                                   cgtaAATCGTCTTCACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCTGCAACACAGGCGTAGCATAc                                <  1:1142865/100‑1 (MQ=255)
                                                                   cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAc                                >  2:2870179/1‑100 (MQ=255)
                                                                    gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                               <  2:631816/100‑1 (MQ=255)
                                                                    gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                               >  2:1737036/1‑100 (MQ=255)
                                                                    gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                               >  2:2635274/1‑100 (MQ=255)
                                                                      aaaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGt                             >  1:2356633/1‑100 (MQ=255)
                                                                        aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                           <  1:246856/100‑1 (MQ=255)
                                                                          cGTCTTAACAACCCTCGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                         <  1:2885587/100‑1 (MQ=255)
                                                                           ggcTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                        <  2:1179797/98‑1 (MQ=255)
                                                                           gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                        >  2:2373461/1‑100 (MQ=255)
                                                                           gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                        >  2:2389679/1‑100 (MQ=255)
                                                                           gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa                        >  2:2792048/1‑100 (MQ=255)
                                                                               tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATg                    >  2:2394510/1‑100 (MQ=255)
                                                                                aacaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgt                   <  1:952075/100‑1 (MQ=255)
                                                                                 acaacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGCACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgta                  >  1:2850751/1‑100 (MQ=255)
                                                                                   aacCCTGGCCCGGTACACATGTGACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtagt                <  1:2614795/100‑1 (MQ=255)
                                                                                       cTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgg            <  1:516816/100‑1 (MQ=255)
                                                                                        tGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg           >  1:896739/1‑100 (MQ=255)
                                                                                         ggCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg          <  2:1463278/100‑1 (MQ=255)
                                                                                         ggCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg          >  2:2847560/1‑100 (MQ=255)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         >  1:1338072/1‑100 (MQ=255)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         >  2:2573179/1‑100 (MQ=255)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         >  1:1339795/1‑100 (MQ=255)
                                                                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         <  2:1640108/100‑1 (MQ=255)
                                                                                          gCCCGGTAACATGTGTACTGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         >  1:2138141/1‑100 (MQ=255)
                                                                                          gCCCGGTAACATGTGTACCGGGCCTACCACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         >  2:1835758/1‑100 (MQ=255)
                                                                                          gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg         >  2:1893323/1‑100 (MQ=255)
                                                                                           cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg        >  1:2743338/1‑100 (MQ=255)
                                                                                           cccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggggg        >  2:1461564/1‑100 (MQ=255)
                                                                                            ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc       >  2:2023217/1‑100 (MQ=255)
                                                                                            ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc       <  2:1041851/100‑1 (MQ=255)
                                                                                            ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGc       >  1:2035258/1‑100 (MQ=255)
                                                                                                tACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCatat   <  1:2259366/100‑1 (MQ=255)
                                                                                                 acacATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg  >  2:2052592/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATG  >  NC_000913/1868761‑1868957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: