Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,645,886 (C)5→4 100% coding (1440/2313 nt) pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,645,8820C.95.8% 513.2 / 13.6 118coding (1444/2313 nt)pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base . (64/49);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 139 total aligned reads displayed.

TGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTG  >  NC_000913/2645783‑2645977
                                                                                                   |                                                                                               
tGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcgccgc                                                                                                 <  1:2034813/100‑1 (MQ=255)
tGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcgccgc                                                                                                 >  2:154595/1‑100 (MQ=255)
 ggTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACgccgcc                                                                                                <  2:2475041/100‑1 (MQ=255)
 ggTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACgccgcc                                                                                                <  1:1163813/100‑1 (MQ=255)
   tGCCTGTGTTGCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATACGGCAAGGGTTGCGCGTCATCAGCGATAATCCGGCGAATGCTCCACGCCGgccc                                                                                              >  1:1359524/1‑100 (MQ=255)
   tGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGcccc                                                                                              <  1:219531/100‑1 (MQ=255)
   tGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGcccc                                                                                              >  1:1729419/1‑100 (MQ=255)
   tGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGcccc                                                                                              >  2:730282/1‑100 (MQ=255)
     cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGTCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccg                                                                                             >  1:729407/1‑98 (MQ=255)
     cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccga                                                                                            <  2:2552860/100‑3 (MQ=255)
     cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccg                                                                                             >  2:29485/1‑98 (MQ=255)
      ccGTTTTCCATGCCAGTGGGGCGACTCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgac                                                                                           <  2:848611/100‑4 (MQ=255)
      ccGTTTTCCATGCCAGGGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgac                                                                                           <  1:1229022/100‑4 (MQ=255)
      ccGTTTTCCATCCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccgac                                                                                           >  1:1522392/1‑97 (MQ=255)
          tttCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACa                                                                                      <  1:2679075/100‑1 (MQ=255)
           ttCTATGCTAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCATGCCGCCCCGACGACAt                                                                                     <  1:1997629/100‑1 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACaat                                                                                    >  2:2700689/1‑97 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTa                                                                                   >  2:1767378/1‑100 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTa                                                                                   >  1:1841025/1‑100 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTa                                                                                   >  1:631889/1‑100 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTa                                                                                   >  2:328372/1‑100 (MQ=255)
             ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGATGCGCTTCATCAGCCATAATCCGGCGACTGATCCACGCCGCCCCgacgacc                                                                                      >  1:1904429/1‑96 (MQ=255)
              cATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCCGCCCTAATCCGGCGAATGATCCACGCCGCCCCGACGACATTaa                                                                                  >  1:2902367/1‑100 (MQ=255)
              cATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTaa                                                                                  >  2:1875926/1‑100 (MQ=255)
              cATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTaa                                                                                  >  2:2333262/1‑100 (MQ=255)
                 gCCAGTGGGGCGACGCGCGGCAAGGCACTATCCTGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAgg                                                                               <  2:2620906/100‑1 (MQ=255)
                 gCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAg                                                                                >  1:1196724/1‑99 (MQ=255)
                  ccAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGc                                                                              <  1:2877853/100‑1 (MQ=255)
                  ccAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGc                                                                              >  2:850579/1‑100 (MQ=255)
                   cAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCTACGACATTAAAGGc                                                                              <  1:2486337/99‑1 (MQ=255)
                     gTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCCCTTCATCAGACATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGcc                                                                             <  1:560744/98‑1 (MQ=255)
                       ggggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTc                                                                         <  1:59108/100‑1 (MQ=255)
                       ggggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTc                                                                         <  2:1256012/100‑1 (MQ=255)
                       ggggCGACGCGCGGCAAGGCACTATCCGCCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGAGCCACGCCGCCCCCGACGACATTAAAGGCCGtt                                                                          <  2:29400/100‑1 (MQ=255)
                        gggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCa                                                                        >  1:1005526/1‑100 (MQ=255)
                           cGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAagc                                                                     >  2:358691/1‑100 (MQ=255)
                           cGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAagc                                                                     <  2:652529/100‑1 (MQ=255)
                              cgcgcgACAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAACCCGGCGAATGATCCACGCCGCCCCGACCACATCAAAGGCCGCCCAagcagc                                                                  >  2:1042759/1‑100 (MQ=255)
                                 gcgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGa                                                               >  2:1525141/1‑100 (MQ=255)
                                  cgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGAt                                                              <  2:672834/100‑1 (MQ=255)
                                  cgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGAt                                                              >  2:1724247/1‑100 (MQ=255)
                                   ggCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATc                                                             >  2:2908777/1‑100 (MQ=255)
                                   ggCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCAGAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGagc                                                             >  2:454679/1‑98 (MQ=255)
                                    gCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCg                                                            <  2:333029/100‑1 (MQ=255)
                                    gCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGACCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCg                                                            <  2:1675489/100‑1 (MQ=255)
                                        ggCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAg                                                        <  2:801202/100‑1 (MQ=255)
                                        ggCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAg                                                        >  1:1967960/1‑100 (MQ=255)
                                         gCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCGGCGGATCGTCAg                                                        <  2:1907413/99‑1 (MQ=255)
                                         gCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAgg                                                       <  1:1127092/100‑1 (MQ=255)
                                          cACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGc                                                      >  1:893844/1‑100 (MQ=255)
                                          cACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGc                                                      <  1:461695/100‑1 (MQ=255)
                                          cACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGc                                                      >  1:1102000/1‑100 (MQ=255)
                                             tATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGt                                                   >  1:222932/1‑100 (MQ=255)
                                              aTCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTa                                                  <  2:115096/100‑1 (MQ=255)
                                                ccGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAg                                                <  1:1249185/100‑1 (MQ=255)
                                                 cGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAg                                                >  1:2217532/1‑99 (MQ=255)
                                                  ggCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgcg                                              <  2:2134649/100‑1 (MQ=255)
                                                  ggCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgcg                                              >  2:2342217/1‑100 (MQ=255)
                                                         gTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtg                                       >  2:2166095/1‑100 (MQ=255)
                                                           tGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcc                                     <  2:1298182/100‑1 (MQ=255)
                                                           tGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcc                                     <  1:872223/100‑1 (MQ=255)
                                                            gcgcTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAGTTtgcct                                    <  2:2587539/100‑1 (MQ=255)
                                                             cgcTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcctg                                   >  2:735/1‑100 (MQ=255)
                                                              gcTTCATCAGCCATAATCCGGCGCATGATCCCCGCCGCCCCGACGACGTTAAAGGCCGTTCAAGCAGCGTATCGTCAGGCTGTAATCGCAATTtgcctn                                   <  1:510079/99‑2 (MQ=255)
                                                                ttCGTCAGCCATAATCCGCCGAATTATCCGCGCCGGCCCGACGACATTAAAGGCCTTTCAGGCAGCGGATCGTCAGGCTGTAAGCGCAATTTgcctgcct                                <  1:1640329/100‑1 (MQ=255)
                                                                 tcatcaGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCtt                               >  2:2196674/1‑100 (MQ=255)
                                                                   atcaGCCATAATCCGGCGAATGATCCACGCCGCCCCGACTACATTAAAGGCCGTTCAAGCAGCGGATCGCCAGGCTGTAAGCGCAATTtgcctg                                   >  1:1085672/1‑94 (MQ=255)
                                                                    tcaGCCATACTCCGGCGAATGATCCACGCCGCACCGACGACATTAAAGGCGTTTCAATCAGCGCATCGTCAGTCTGTAAGCGCAATTTGCCTGCCTTGc                             >  1:1258093/1‑99 (MQ=255)
                                                                    tcaGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGcc                            >  1:2159965/1‑100 (MQ=255)
                                                                     caGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCg                           <  2:12182/100‑1 (MQ=255)
                                                                      aGCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGt                          <  2:193266/100‑1 (MQ=255)
                                                                       gCCATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTg                         >  1:1600217/1‑100 (MQ=255)
                                                                        ccATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTg                         <  2:453438/100‑1 (MQ=255)
                                                                         cATAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGc                       <  1:1015355/100‑1 (MQ=255)
                                                                          aTAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      <  1:2074252/100‑1 (MQ=255)
                                                                          aTAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg                      <  1:2387021/100‑1 (MQ=255)
                                                                           tAATCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGa                     >  2:2104397/1‑100 (MQ=255)
                                                                            agtCCGGCGAATGATCCACGCCGCCCCGACGACATGAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    <  2:166305/98‑1 (MQ=255)
                                                                            aagcaggCGAATGATCCACGCCGCCACCACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg                    <  1:1299551/95‑1 (MQ=255)
                                                                             aTCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGc                   <  2:2785696/100‑1 (MQ=255)
                                                                             aTCCGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGc                   >  2:792769/1‑100 (MQ=255)
                                                                              tCCGGCGAATGATCCACGCCGCCCCGACGACATTAGAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCa                  >  1:223404/1‑100 (MQ=255)
                                                                               ccGGCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTCCCGTGGCGAGCaa                 >  1:1711762/1‑100 (MQ=255)
                                                                               ccGGCGAATGATCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCaaa                <  1:476476/100‑1 (MQ=255)
                                                                                cGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCaa                 >  2:806475/1‑100 (MQ=255)
                                                                                 ggCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAAc               >  2:666729/1‑100 (MQ=255)
                                                                                  gCGAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAAcg              >  1:674307/1‑100 (MQ=255)
                                                                                    gAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAAcgcg            >  1:978416/1‑100 (MQ=255)
                                                                                    gAATGATCCACGCCGCCCCGACGACATTAAAGGCCGTTAAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAAcgcg            >  1:1311641/1‑100 (MQ=255)
                                                                                     aaTGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCgg           <  1:1836833/100‑1 (MQ=255)
                                                                                      aTGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGt          >  1:1048262/1‑100 (MQ=255)
                                                                                      aTGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGt          <  1:2299992/100‑1 (MQ=255)
                                                                                       tGATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGta         <  2:1375609/100‑1 (MQ=255)
                                                                                       tGAGCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGt          <  1:1711515/99‑1 (MQ=255)
                                                                                        gATCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGtat        >  2:1153731/1‑100 (MQ=255)
                                                                                         aTCCACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGtata       >  2:2241583/1‑100 (MQ=255)
                                                                                          tCCACGCCGCCCCGACGACATTACAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATAc      >  2:276685/1‑100 (MQ=255)
                                                                                            cACGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGACCGCCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATCCGc    >  1:1325982/1‑100 (MQ=255)
                                                                                             aCGCCGCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCt   <  2:2381095/100‑1 (MQ=255)
                                                                                              cgccgcCCCGACGACATTGAAGGCCGTTTAAGCAGCGGATCGGCGGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGTCGAGCAAAGGCGGtatagg     >  1:965537/1‑95 (MQ=255)
                                                                                                   |                                                                                               
TGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTG  >  NC_000913/2645783‑2645977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: