| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,446,357:1 | +T | 17.1% | intergenic (+151/+21) | ydbL → / ← feaR | DUF1318 family protein/transcriptional activator for tynA and feaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,446,357 | 1 | . | T | 17.1% | 233.0 / 52.5 | 76 | intergenic (+151/+21) | ydbL/feaR | DUF1318 family protein/transcriptional activator for tynA and feaB |
| Reads supporting (aligned to +/- strand): ref base . (34/29); new base T (4/9); total (38/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-01 | |||||||||||
TTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA > NC_000913/1446259‑1446456 | ttttcacaaaCAACGTGACTATTCAAAAACTCCTTTGAATTTGCCTTTTGCCCTGAAAAATGCATAGTCTAATATCTGTTTTAACTAATTGGCGTTGCA‑g < 1:2615181/93‑1 (MQ=255)tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑g > 2:357792/1‑100 (MQ=255) ttcTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTa < 2:1499065/98‑1 (MQ=255) tCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTa < 2:2352311/100‑1 (MQ=255) tCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTa > 2:2322733/1‑100 (MQ=255) ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTAc > 2:1325963/1‑100 (MQ=255) ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTAc > 2:2374237/1‑100 (MQ=255) ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTAc > 2:2374015/1‑100 (MQ=255) cTGAAAAAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACa < 2:953898/100‑1 (MQ=255) gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGTCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATg < 1:2027285/100‑1 (MQ=255) aaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCa > 2:922505/1‑100 (MQ=255) aaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCa > 2:1785140/1‑100 (MQ=255) cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAAc > 1:2572524/1‑100 (MQ=255) cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAAc < 2:2753743/100‑1 (MQ=255) aaGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACg < 2:2277678/100‑1 (MQ=38) aaGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACg < 2:1603042/100‑1 (MQ=38) ggTGAATATTCAAAAGCTCCTGTCAAATTGCTTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGcc < 1:1917818/100‑1 (MQ=37) ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGcc < 2:2694362/100‑1 (MQ=38) ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGcc < 1:2564331/100‑1 (MQ=38) ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGcc < 2:117315/100‑1 (MQ=255) ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGcc < 2:361971/100‑1 (MQ=255) gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCa > 2:185081/1‑100 (MQ=255) tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa > 1:1662210/1‑100 (MQ=255) tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa > 2:1390188/1‑100 (MQ=255) tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCACTGAAAAATGTATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGCCa < 1:173473/99‑1 (MQ=37) gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACg < 1:1682610/95‑1 (MQ=38) gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGCCAAt < 2:1851342/100‑1 (MQ=38) gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAt > 2:2904175/1‑100 (MQ=255) gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATAGCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa > 1:526720/1‑99 (MQ=255) aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCTCTGAAAAATGCGTAGTCAAATATCTGTTTTAACTAACTGGCGTTGCA‑GTACATGCAACGCCAAtt < 1:1814719/100‑1 (MQ=255) tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAGCGCCAAtt < 1:2731238/98‑1 (MQ=255) tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAttag < 2:2205902/100‑1 (MQ=255) cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGCCAAttagttaa < 1:1541325/100‑2 (MQ=37) cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAttagttag < 1:2243792/100‑1 (MQ=255) aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGc < 2:1367331/100‑1 (MQ=255) aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGc > 2:562967/1‑100 (MQ=255) aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCg < 1:1978022/100‑1 (MQ=255) aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCg > 2:2246868/1‑100 (MQ=255) aaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCgg < 1:324848/100‑1 (MQ=255) aaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGa > 1:2407928/1‑100 (MQ=255) cTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTa > 2:2093847/1‑100 (MQ=255) tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTAc > 2:2361102/1‑100 (MQ=255) cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGTAATTTACGTc < 1:185079/100‑1 (MQ=255) aaaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCg > 1:525365/1‑100 (MQ=255) ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa > 2:2412275/1‑100 (MQ=255) gCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACt < 2:2828365/100‑1 (MQ=255) gCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACt > 1:2026719/1‑100 (MQ=255) ccTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTc > 2:640595/1‑100 (MQ=255) cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg < 2:2395481/100‑1 (MQ=255) gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTgg > 2:1948044/1‑100 (MQ=255) cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACACCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGt > 1:1248037/1‑100 (MQ=255) tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTc > 1:1597636/1‑100 (MQ=255) tGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCaa < 2:2155260/100‑1 (MQ=255) cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg > 2:2491881/1‑100 (MQ=255) cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg < 1:775445/100‑1 (MQ=255) cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg > 2:2109204/1‑100 (MQ=255) tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg > 2:1288874/1‑100 (MQ=255) gTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt > 2:2788139/1‑100 (MQ=255) gTCAAATATCTGTGTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt > 2:1954284/1‑100 (MQ=255) cAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc < 1:1472969/100‑1 (MQ=255) cAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc < 2:1077401/100‑1 (MQ=255) aaaTATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTGCGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt > 2:1030722/1‑100 (MQ=255) tatCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaa < 1:1558416/100‑1 (MQ=255) tatCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaa < 1:1697865/100‑1 (MQ=255) tatCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaa < 1:2412264/100‑1 (MQ=255) atCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaa > 2:1579189/1‑99 (MQ=255) cgctAACTAATTGGCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg > 1:350311/4‑100 (MQ=25) tttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg < 2:578343/99‑1 (MQ=255) tttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt < 1:386247/100‑1 (MQ=255) ttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGtt < 2:2890823/100‑1 (MQ=255) aaTTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa > 2:2183686/1‑100 (MQ=255) ttGGCGTTGCA‑GTACATGCACCGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg > 1:93168/1‑100 (MQ=255) ggCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc > 1:2225602/1‑100 (MQ=38) ggCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc > 2:2312941/1‑100 (MQ=38) ggCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc > 2:995163/1‑100 (MQ=255) ggCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc < 1:1402589/100‑1 (MQ=255) gCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAct > 2:772329/1‑100 (MQ=38) gCGTCGCA‑GTACCTCCTACGCCACTCAGTTAGCGGAATTTATGTCGATCCTCGCCTGGCGTCATGCCAAAGCGTTTCTTAAATACCGTTGAAAAATGAc < 1:325356/99‑1 (MQ=255) a‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa > 2:326101/1‑100 (MQ=255) a‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa > 1:1726932/1‑100 (MQ=255) | TTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA > NC_000913/1446259‑1446456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |