Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,181,298 A→T 15.4% H293L (CAT→CTT)  ygiC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,181,2980AT15.4% 332.0 / 49.2 130H293L (CAT→CTT) ygiCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base A (60/49);  new base T (7/13);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 139 total aligned reads displayed.

GGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGG  >  NC_000913/3181199‑3181397
                                                                                                   |                                                                                                   
ggAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatca                                                                                                     <  2:1276456/100‑1 (MQ=255)
 gAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatcat                                                                                                    >  2:1418248/1‑100 (MQ=255)
  nagagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGatcatc                                                                                                   >  1:2585706/2‑100 (MQ=255)
   agagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  <  1:2283431/100‑1 (MQ=255)
   agagCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  >  1:2082559/1‑100 (MQ=255)
   agagCATTATCTCCAACAAGGCACTTCTACCGCTACTATGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATcc                                                                                                  >  2:1588690/1‑100 (MQ=255)
     agCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGc                                                                                                >  2:2883539/1‑100 (MQ=255)
     agCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGc                                                                                                <  2:2700715/100‑1 (MQ=255)
      gCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCa                                                                                               >  1:2560539/1‑100 (MQ=255)
      gCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCa                                                                                               >  1:1708437/1‑100 (MQ=255)
       gatcttctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaa                                                                                              <  2:1664811/95‑1 (MQ=255)
       cATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaa                                                                                              <  2:1423332/100‑1 (MQ=255)
        aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaa                                                                                             <  2:2393950/100‑1 (MQ=255)
        aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaa                                                                                              >  2:882304/1‑99 (MQ=255)
        aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGCAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCaaa                                                                                             <  1:1603749/100‑1 (MQ=255)
         ttATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaa                                                                                            <  2:7104/100‑2 (MQ=39)
         ttATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaa                                                                                            <  2:1675412/100‑2 (MQ=39)
          tctctcCAACAAGGCACTTCTACCGCTACGGGGGGAGATGTTCCCGAATCGCCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaat                                                                                           <  1:1759573/98‑3 (MQ=37)
          tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATg                                                                                           <  1:470539/100‑1 (MQ=255)
          tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATg                                                                                           <  2:2269729/100‑1 (MQ=255)
          tATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGATTATTTTGCGGATGATCTTCTTCCGCAAAtt                                                                                           <  2:1961553/100‑2 (MQ=255)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaata                                                                                          <  1:2768635/100‑4 (MQ=38)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaata                                                                                          <  2:137937/100‑4 (MQ=38)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATgg                                                                                          >  1:886005/1‑100 (MQ=255)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATgg                                                                                          >  2:1628116/1‑100 (MQ=255)
           aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATgg                                                                                          <  1:2157105/100‑1 (MQ=255)
            tctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaagatc                                                                                         >  1:1664806/1‑96 (MQ=255)
            tctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGa                                                                                         >  2:2378343/1‑100 (MQ=255)
             ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAACTGGaa                                                                                        >  2:1126603/1‑100 (MQ=255)
             ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGACGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGaa                                                                                        >  1:188706/1‑100 (MQ=255)
               ccAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGATGATCATCTTCCGCaaaataagcg                                                                                      <  2:841287/100‑8 (MQ=37)
                  acaaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAata                                                                                   <  2:1013846/100‑1 (MQ=255)
                    aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtg                                                                                 <  2:1273960/100‑1 (MQ=255)
                    aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtg                                                                                 <  1:2902675/100‑1 (MQ=255)
                     aGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATAtgt                                                                                >  2:27573/1‑100 (MQ=255)
                        cACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGt                                                                             >  1:194632/1‑100 (MQ=255)
                         aCTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGtt                                                                            >  1:419745/1‑100 (MQ=255)
                          cTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTa                                                                           >  1:2529805/1‑100 (MQ=255)
                          cTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTa                                                                           >  2:1050968/1‑100 (MQ=255)
                            tCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCAGCTTATTTTGCGGAAGATGATCATCCGCAAAAGTAAAAATATGTGGTTaaa                                                                         >  1:1710714/1‑100 (MQ=255)
                             cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAc                                                                        <  1:2208431/100‑1 (MQ=255)
                             cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAc                                                                        <  2:313547/100‑1 (MQ=255)
                             cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGCGGTTAAAc                                                                        <  1:1628112/100‑1 (MQ=255)
                              tACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAAcc                                                                       <  2:968397/100‑1 (MQ=255)
                               aCCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCg                                                                      >  2:643573/1‑100 (MQ=255)
                                ccGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGa                                                                     >  1:346176/1‑100 (MQ=255)
                                  gCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtc                                                                   >  2:208313/1‑100 (MQ=255)
                                   cTACTGTGGGAGATGTTCCCGAATTACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGAtct                                                                  <  2:2611752/100‑1 (MQ=255)
                                       tgtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTtctc                                                              >  1:1357905/1‑100 (MQ=255)
                                       tgtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTtctc                                                              >  2:587505/1‑100 (MQ=255)
                                        gtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTtctc                                                              >  1:1951205/1‑99 (MQ=255)
                                        gtgGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTcc                                                             <  2:1926117/100‑1 (MQ=255)
                                          gggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCg                                                           >  1:1470072/1‑100 (MQ=255)
                                          gggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCg                                                           <  1:1089612/100‑1 (MQ=255)
                                           ggAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGt                                                          <  2:741866/100‑1 (MQ=255)
                                              gaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGaa                                                       <  1:2730502/100‑1 (MQ=255)
                                                tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     <  2:2197342/100‑1 (MQ=255)
                                                tGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAgg                                                     <  2:486331/100‑1 (MQ=255)
                                                 gTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgc                                                    <  1:1012524/100‑1 (MQ=255)
                                                 gTTCCCGAATCACCCGAACCTGCTGACCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTGAACCGATCTTCTCCCGTGAAgg                                                     <  1:1212467/99‑1 (MQ=255)
                                                  ttCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcg                                                   >  1:1491754/1‑100 (MQ=255)
                                                   tCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcgc                                                  >  2:250299/1‑100 (MQ=255)
                                                    cccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGgcgc                                                  >  1:786103/1‑99 (MQ=255)
                                                    cccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCa                                                 <  2:2826549/100‑1 (MQ=255)
                                                    cccGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCa                                                 >  2:889959/1‑100 (MQ=255)
                                                     ccGAATCACCCGAACCTGCTACCCGCTTATTTTGCAGAAGATGATCATCCGCAACTGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCaa                                                >  2:1732688/1‑100 (MQ=255)
                                                        aaTCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACg                                             <  2:1083510/100‑1 (MQ=255)
                                                            aCCCGAACCTGCTGCACGCTTATTTTGCGGAAGATGATAAAAAAAAAAAAAAAAAATATGTGGTTAAACCGATCTTCTCTCGTGAAGGCGCAAACGTGTc                                         <  1:1782910/100‑1 (MQ=255)
                                                             cccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCg                                        >  1:401460/1‑100 (MQ=255)
                                                              ccGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAATCGTGTc                                         <  1:2139959/98‑1 (MQ=255)
                                                                   ccTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCAtt                                  >  1:1898802/1‑100 (MQ=255)
                                                                    ctgctgCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTg                                 <  2:234675/100‑1 (MQ=255)
                                                                     tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                <  1:533733/100‑1 (MQ=255)
                                                                     tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                >  2:939354/1‑100 (MQ=255)
                                                                       ctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATCTGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTgaga                              >  1:886813/1‑100 (MQ=255)
                                                                       ctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTgaga                              >  2:347078/1‑100 (MQ=255)
                                                                       ctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTgaga                              <  1:645954/100‑1 (MQ=255)
                                                                            cGCTTATTTTGGCGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTACTCCAGTGAAGGCGCAAACGTGTCAATCATTGAGAACg                           <  1:1785347/98‑1 (MQ=255)
                                                                             gCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCa                        >  1:1689910/1‑100 (MQ=255)
                                                                               ttATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaa                      <  2:2156913/100‑1 (MQ=255)
                                                                                tATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                     >  1:1877468/1‑100 (MQ=255)
                                                                                tATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                     >  1:244574/1‑100 (MQ=255)
                                                                                tATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                     <  1:2693289/100‑1 (MQ=255)
                                                                                ccatttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCaaaa                     >  1:1217255/4‑100 (MQ=38)
                                                                                 catttGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCCAAAc                    >  1:986256/3‑100 (MQ=38)
                                                                                 aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTTGATCATTGAGAACGGCAAAAc                    >  1:1496618/1‑100 (MQ=255)
                                                                                   tttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCa                  <  2:1576636/100‑1 (MQ=255)
                                                                                    ttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACATGTCGATCATTGAGAACGGCAAAACCAt                 >  2:2448783/1‑100 (MQ=255)
                                                                                     tGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAtt                >  2:672915/1‑100 (MQ=255)
                                                                                     tGCGGATGATCATCTTCCGCAAATGGAAAAATATGTGGTTAAAACGATCTTCTCCCGTGAAGGCGCAAACGTGGCGATCATTGAGAACGGCAAAACCAt                 >  1:1079029/1‑99 (MQ=255)
                                                                                     tGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCAtt                <  2:2487052/100‑1 (MQ=255)
                                                                                        ggAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGaa             <  2:194634/100‑1 (MQ=255)
                                                                                         gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGaa             <  1:2600233/99‑1 (MQ=255)
                                                                                              tgatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCg       >  1:267113/1‑100 (MQ=255)
                                                                                               gatCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCgg      >  1:2851398/1‑100 (MQ=255)
                                                                                                atcatcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGa     >  1:999494/1‑100 (MQ=255)
                                                                                                 tcatcCGCAAATGGAAAAATATGTGGTTAATCCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa    <  2:235200/100‑1 (MQ=255)
                                                                                                 tcatcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa    >  1:1443700/1‑100 (MQ=255)
                                                                                                   atcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAgg  <  1:1581099/100‑1 (MQ=255)
                                                                                                   atcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAgg  >  1:1055231/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GGAAGAGCATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGG  >  NC_000913/3181199‑3181397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑