Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,790,300 A→T 15.8% intergenic (‑219/+20) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,3000AT15.8% 325.4 / 44.9 125intergenic (‑219/+20)waaH/tdhLPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (51/54);  new base T (7/13);  total (58/67)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 131 total aligned reads displayed.

TGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGA  >  NC_000913/3790201‑3790399
                                                                                                   |                                                                                                   
tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGa                                                                                                     <  2:213971/100‑1 (MQ=255)
tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGa                                                                                                     <  1:903384/100‑1 (MQ=255)
  agagCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAAt                                                                                                   <  2:857221/100‑1 (MQ=255)
  agagCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAAt                                                                                                   >  1:2876184/1‑100 (MQ=255)
      cagcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAccn                                                                                               <  1:1396707/100‑2 (MQ=255)
       agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                              <  2:1637283/100‑1 (MQ=255)
       agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg                                                                                              <  1:2789837/100‑1 (MQ=255)
          tatataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGccc                                                                                           <  1:1017990/100‑1 (MQ=255)
          tatataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGccc                                                                                           <  2:2445640/100‑1 (MQ=255)
          tatataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGccc                                                                                           <  2:157802/100‑1 (MQ=255)
           atataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTTCATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCt                                                                                          <  2:684562/100‑1 (MQ=255)
             ataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttt                                                                                        <  1:306924/100‑2 (MQ=255)
             ataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttt                                                                                        <  2:2653839/100‑2 (MQ=255)
              taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCtttt                                                                                       <  1:2163855/100‑3 (MQ=255)
              taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGt                                                                                       <  2:2339246/100‑1 (MQ=255)
              taAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGt                                                                                       >  1:1872823/1‑100 (MQ=255)
               aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                      <  2:2794847/100‑1 (MQ=255)
               aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGtt                                                                                      >  2:2193706/1‑100 (MQ=255)
                agagATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttttat                                                                                     <  1:100977/100‑5 (MQ=39)
                 gatagcAGCACAATTACGATCTCTTTATTACACTGAAAGGATTGTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCttttatc                                                                                    <  1:1319106/95‑6 (MQ=37)
                   gaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtg                                                                                  >  1:2150260/1‑100 (MQ=255)
                   gaTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtg                                                                                  >  1:752958/1‑100 (MQ=255)
                    atcatcACAATTACAAACTCTTGATTACACTGACATGATTTTACCTATTTAACAGATTATCCTCAGATAAAAGGGCGGGTATTCCAGCCCttttatct                                                                                   <  1:85085/98‑7 (MQ=25)
                    aTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTACCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt                                                                                 <  1:1885501/100‑1 (MQ=255)
                    aTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt                                                                                 <  2:2204714/100‑1 (MQ=255)
                    aTCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt                                                                                 <  1:1389186/100‑1 (MQ=255)
                     tCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGtt                                                                                <  2:1817609/100‑1 (MQ=255)
                     tCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGtt                                                                                <  2:1492978/100‑1 (MQ=255)
                     tCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGtt                                                                                <  2:902195/100‑1 (MQ=255)
                       aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                                                              >  1:2117827/1‑100 (MQ=255)
                        gCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAAt                                                                             >  1:2514511/1‑100 (MQ=255)
                         cacaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATc                                                                            <  2:517265/100‑1 (MQ=255)
                          tcaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATcc                                                                           <  2:1449100/99‑1 (MQ=255)
                          acaATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATcc                                                                           >  1:2398258/1‑100 (MQ=255)
                           caATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATcca                                                                          >  1:1449097/1‑99 (MQ=255)
                            aaTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCa                                                                         <  2:2151597/100‑1 (MQ=255)
                             aTTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATccc                                                                          >  1:1176004/1‑98 (MQ=255)
                              ttACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGc                                                                       <  2:495923/100‑1 (MQ=255)
                                aCGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTc                                                                     >  2:1716393/1‑100 (MQ=255)
                                aCGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTc                                                                     >  2:347642/1‑100 (MQ=255)
                                 cGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCa                                                                    >  1:181830/1‑100 (MQ=255)
                                 cGAACTCTTTATTAAAATAAAAGGATTTTAAATATTTAACATATTATCCTCAGATAAAAGAGATAGAATACAAGTCATTATTCGTGTTAATccaaaat                                                                      >  1:769314/1‑93 (MQ=255)
                                  gAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCan                                                                   <  2:2859965/100‑2 (MQ=255)
                                   aaCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGa                                                                  >  1:1066910/1‑100 (MQ=255)
                                      tctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATa                                                               <  1:1952440/100‑1 (MQ=255)
                                        tgtattaCACTGACAGGATTTTACATATTTCACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAAc                                                             <  1:1770103/98‑1 (MQ=255)
                                           attaCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACttt                                                          >  2:1627247/1‑100 (MQ=255)
                                             taCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTcc                                                        >  2:1636325/1‑100 (MQ=255)
                                              acacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTccc                                                       >  2:2242915/1‑100 (MQ=255)
                                              acacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCACTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTccc                                                       >  1:2790009/1‑100 (MQ=255)
                                                acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCgg                                                     <  2:1141155/100‑1 (MQ=255)
                                                acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCgg                                                     >  1:1065402/1‑100 (MQ=255)
                                                   gACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACt                                                  <  2:2753614/100‑1 (MQ=255)
                                                     cAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTgg                                                <  2:2636905/100‑1 (MQ=255)
                                                     cAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTgg                                                >  2:186745/1‑100 (MQ=255)
                                                     cAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTgg                                                >  2:1654737/1‑100 (MQ=255)
                                                       ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                              <  2:2086558/100‑1 (MQ=255)
                                                       ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                              >  2:350489/1‑100 (MQ=255)
                                                       ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                              <  1:442685/100‑1 (MQ=255)
                                                       ggATTTTACATATGTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc                                              <  2:2719400/100‑1 (MQ=255)
                                                        gATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGccc                                             <  2:2035575/100‑1 (MQ=255)
                                                             tACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg                                        <  1:842498/100‑1 (MQ=255)
                                                             tACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCAAGCTCAGAATAACTTTCGCGGACTGGCTCGAACg                                        >  1:1698784/1‑100 (MQ=255)
                                                               cATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCa                                      <  2:457480/100‑1 (MQ=255)
                                                               cATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCa                                      <  2:161298/100‑1 (MQ=255)
                                                                  atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAg                                   >  1:480129/1‑100 (MQ=255)
                                                                  atTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAg                                   >  2:1594317/1‑100 (MQ=255)
                                                                    ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCg                                 <  2:660230/100‑1 (MQ=255)
                                                                     tAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGt                                >  2:1869119/1‑100 (MQ=255)
                                                                     tAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGt                                >  2:278026/1‑100 (MQ=255)
                                                                     tAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCATTGTTCGTGTTAATCCCAGCTCAGAATAGCTTTCCCGGACTGGCCCGAACGCATAGCg                                 <  1:1609276/99‑1 (MQ=255)
                                                                         aGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCGTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCaaa                            >  1:1552443/1‑100 (MQ=255)
                                                                            ttATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGcc                         <  2:374578/100‑1 (MQ=255)
                                                                               tcctccGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATAACAGCTAAGAATAACTTTCCCAAACTGGCCCGAACGCATAGCGTCAACGCCCtt                      >  1:1281041/1‑100 (MQ=255)
                                                                                taacacgaacaaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTc                     >  2:2491971/11‑100 (MQ=37)
                                                                                taacacgaacaaGGGCTGGTATTCCAGCCCTTGTGCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTc                     <  2:2177513/90‑1 (MQ=37)
                                                                                  tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTg                   >  1:249400/1‑100 (MQ=255)
                                                                                   cAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTgg                  <  1:157061/100‑1 (MQ=255)
                                                                                     gATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaa                >  2:1477421/1‑100 (MQ=255)
                                                                                      aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaaa               <  1:694985/100‑1 (MQ=255)
                                                                                      aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaaa               <  1:1744495/100‑1 (MQ=255)
                                                                                      aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaaa               <  2:2257582/100‑1 (MQ=255)
                                                                                      aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaaa               <  2:549406/100‑1 (MQ=255)
                                                                                       tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAat              <  1:417944/100‑1 (MQ=255)
                                                                                       tAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACCGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGaa                >  1:2211242/1‑98 (MQ=255)
                                                                                         aaaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatca            >  1:2570942/1‑100 (MQ=255)
                                                                                         aaaGGGCTGGAATACCAGCCCTGGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAAAGCCTAGCGTCAAAGCCCTTCTGGAAatca            >  1:2637401/1‑100 (MQ=255)
                                                                                          aaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcat           >  1:2211820/1‑100 (MQ=255)
                                                                                          aaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcat           >  2:1074484/1‑100 (MQ=255)
                                                                                           aGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcatc          >  2:2401139/1‑100 (MQ=255)
                                                                                           aGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcatc          >  2:1550468/1‑100 (MQ=255)
                                                                                           aGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAatcatc          <  1:1920248/100‑1 (MQ=255)
                                                                                            gggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCg         <  1:512368/100‑1 (MQ=255)
                                                                                             ggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGa        >  1:738608/1‑100 (MQ=255)
                                                                                             ggCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATAATCg         >  2:1686862/1‑99 (MQ=255)
                                                                                              gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGa        <  1:227767/99‑1 (MQ=255)
                                                                                              gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGAt       >  2:902855/1‑100 (MQ=255)
                                                                                              gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGAt       >  2:1085875/1‑100 (MQ=255)
                                                                                              gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGAt       <  1:1469672/100‑1 (MQ=255)
                                                                                              gCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGAt       >  1:1316869/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGA  >  NC_000913/3790201‑3790399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: