Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,051,259 G→T 16.9% intergenic (‑494/‑88) yihA ← / → yihI cell division GTP‑binding protein/activator of Der GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,2590GT16.9% 315.8 / 48.9 118intergenic (‑494/‑88)yihA/yihIcell division GTP‑binding protein/activator of Der GTPase
Reads supporting (aligned to +/- strand):  ref base G (45/53);  new base T (11/9);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 126 total aligned reads displayed.

CACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCA  >  NC_000913/4051160‑4051358
                                                                                                   |                                                                                                   
cacGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGGTATGAGTCAGGAAAAAAGGCGACagag                                                                                                     >  1:2487581/1‑100 (MQ=255)
 acGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGt                                                                                                    >  1:1338669/1‑100 (MQ=255)
 acGGATTGTACCGGCAAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAAGATTGTAAGACCCGTTAAGGGTAAAAAAAAAAAAAAAAAGGCGACAGAGt                                                                                                    <  1:624402/100‑1 (MQ=255)
  cGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGt                                                                                                    <  2:2728609/99‑1 (MQ=255)
    gATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAAt                                                                                                 <  2:2593643/100‑1 (MQ=255)
     aTTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATc                                                                                                <  2:1446634/100‑1 (MQ=255)
     aTTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATc                                                                                                >  1:2017670/1‑100 (MQ=255)
         ttCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTc                                                                                            <  1:1404044/100‑1 (MQ=255)
          tCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCg                                                                                           >  1:223471/1‑100 (MQ=255)
          tCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCg                                                                                           >  2:2567317/1‑100 (MQ=255)
           ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGc                                                                                          <  2:223473/100‑1 (MQ=255)
            cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                         <  1:799655/100‑1 (MQ=255)
            cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                         <  2:2088786/100‑1 (MQ=255)
            cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGcc                                                                                         >  2:1892551/1‑100 (MQ=255)
            cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGcc                                                                                         >  2:600386/1‑100 (MQ=255)
             ggCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCt                                                                                        <  2:2905416/100‑1 (MQ=255)
             ggCTAAAGGAAAAACAGGGTGGGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCg                                                                                           <  1:2658326/97‑1 (MQ=255)
              gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                       <  2:2336122/100‑1 (MQ=255)
               cTAAAGGAAAAACAGTGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCttt                                                                                      <  2:2856079/100‑1 (MQ=255)
                tAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttt                                                                                     >  2:2592634/1‑100 (MQ=255)
                  aaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttttt                                                                                   >  2:180058/1‑100 (MQ=255)
                   aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTCTGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTc                                                                                  >  2:473615/1‑100 (MQ=255)
                   aGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTc                                                                                  >  1:434846/1‑100 (MQ=255)
                    ggAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtttttt                                                                                   <  1:720237/98‑1 (MQ=255)
                      aaaaaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCttt                                                                               <  1:1998968/100‑1 (MQ=255)
                      aaaaaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCttt                                                                               <  2:2749805/100‑1 (MQ=255)
                      aaaaaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCttt                                                                               <  2:2663832/100‑1 (MQ=255)
                         aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                            <  1:501049/100‑1 (MQ=255)
                         aaCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgct                                                                            >  1:1312020/1‑100 (MQ=255)
                          aCAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgctt                                                                           <  2:1312022/100‑1 (MQ=255)
                            aGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAGGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttgc                                                                         <  2:2199487/100‑1 (MQ=255)
                            aGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTttgcttgc                                                                         <  2:106347/100‑1 (MQ=255)
                                tgtgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTc                                                                     <  2:1747017/100‑1 (MQ=255)
                                   gttgtcgtgcTGCAAGGATTGTAAGACGCGTTAAGGGTTATGAGTGAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTg                                                                   <  2:2718495/90‑1 (MQ=38)
                                   gtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGt                                                                  <  1:1930658/100‑1 (MQ=255)
                                   gtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGt                                                                  >  2:1419060/1‑100 (MQ=255)
                                    ttGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGtt                                                                 <  2:1827487/100‑1 (MQ=255)
                                     tGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                                <  1:180056/100‑1 (MQ=255)
                                      ggcggcCTGCCAGGCTCGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAAGCGACCGAGCAGCCTGTCGCCGTGTTGCTTGGCTTGCTTTCTGTTAg                                                               >  1:2060069/1‑100 (MQ=255)
                                      ggaggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGGGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAg                                                               <  1:1801763/97‑1 (MQ=255)
                                        cggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGAt                                                             >  2:175499/1‑100 (MQ=255)
                                        cggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGAt                                                             >  2:175525/1‑100 (MQ=255)
                                           ccTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAATAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTcc                                                          <  1:1280745/100‑1 (MQ=255)
                                           ccTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTcc                                                          <  1:1040882/100‑1 (MQ=255)
                                           ccTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTcc                                                          <  1:1275186/100‑1 (MQ=255)
                                             tGCAAGGATTGTAAGCCCCGTTAAGTTTTATGATTCAGTAAACAAGGCGACAGCGTAATCTGTCTCCTTTTTTCTTTGCGTGCTTTCTGTTAGATTccgc                                                        <  1:2249619/100‑1 (MQ=255)
                                             tGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgc                                                        <  2:1169735/100‑1 (MQ=255)
                                              gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                       >  2:2226613/1‑100 (MQ=255)
                                              gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                       >  1:2602475/1‑100 (MQ=255)
                                               cAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgccg                                                      <  2:1665603/100‑1 (MQ=255)
                                               cAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgccg                                                      <  2:308485/100‑1 (MQ=255)
                                                aaGGAGTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGGCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCcgccgc                                                     <  1:352847/100‑1 (MQ=255)
                                                  ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGGGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCa                                                    >  1:1054253/1‑99 (MQ=255)
                                                     ttGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATc                                                >  2:1459936/1‑100 (MQ=255)
                                                      tGTAAGACCCTGTACGGGGTATGAGTCAGGAGAAAAGGCGACAGTGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaaagc                                                <  1:558668/99‑3 (MQ=255)
                                                       gTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCta                                              >  2:154011/1‑100 (MQ=255)
                                                       gTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCta                                              >  2:1023723/1‑100 (MQ=255)
                                                        tAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtat                                             >  1:1933468/1‑100 (MQ=255)
                                                         aaGGCCCGTGAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtata                                            <  1:42360/100‑1 (MQ=255)
                                                         aaGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCtata                                            <  1:2273223/100‑1 (MQ=255)
                                                            aCCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTg                                         <  1:1293917/100‑1 (MQ=255)
                                                              ccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGaa                                       <  1:483957/100‑1 (MQ=255)
                                                               cGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAAt                                      <  1:1460586/100‑1 (MQ=255)
                                                                gTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATa                                     >  2:231632/1‑100 (MQ=255)
                                                                  tAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTTTACTGAATaaa                                   >  1:1675200/1‑100 (MQ=255)
                                                                    aGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                                 >  2:680306/1‑100 (MQ=255)
                                                                     gggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACg                                >  2:1306118/1‑100 (MQ=255)
                                                                     gggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACg                                <  1:1589234/100‑1 (MQ=255)
                                                                      ggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACgg                               <  2:1714836/100‑1 (MQ=255)
                                                                       gTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGc                              <  2:1061806/100‑1 (MQ=255)
                                                                           tGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTaaa                          >  1:2607566/1‑100 (MQ=255)
                                                                           tGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAAGCTATACTGAATAAAACGGCTaaa                          >  1:2142744/1‑100 (MQ=255)
                                                                              gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAg                        >  1:172894/1‑99 (MQ=255)
                                                                               tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGAc                      <  2:2017677/100‑1 (MQ=255)
                                                                               tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGAc                      <  1:2163057/100‑1 (MQ=255)
                                                                                cAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACg                     >  2:332289/1‑100 (MQ=255)
                                                                                  ggAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGaa                   >  2:640685/1‑100 (MQ=255)
                                                                                    aaaaaaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAAcc                 >  2:1672275/1‑100 (MQ=255)
                                                                                     aaaaaGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCa                >  2:658295/1‑100 (MQ=255)
                                                                                     aaaaaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCa                >  2:2304883/1‑100 (MQ=255)
                                                                                        aaGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTa             >  1:1151310/1‑100 (MQ=255)
                                                                                        aaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTa             >  2:395966/1‑100 (MQ=255)
                                                                                        aaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTATGTTAGATTGCGACGCAAATCTATACTGAATATAACGGCTAAAAGACGAACCATTa             <  1:842121/100‑1 (MQ=255)
                                                                                         aGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt            >  2:2608374/1‑100 (MQ=255)
                                                                                         aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTTCTTTCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt            >  1:751169/1‑100 (MQ=255)
                                                                                         aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTATATTCCGCCGCAAATCTATACTGAATACAATGGCTAAAAGACGAACCAt               >  1:1205034/1‑97 (MQ=255)
                                                                                         aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt            >  2:796223/1‑100 (MQ=255)
                                                                                          ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt            >  2:2838983/1‑99 (MQ=255)
                                                                                          ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg           >  2:2838982/1‑100 (MQ=255)
                                                                                          ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg           >  2:1683931/1‑100 (MQ=255)
                                                                                          ggCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg           <  2:750760/100‑1 (MQ=255)
                                                                                           gCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGa          >  2:2854945/1‑100 (MQ=255)
                                                                                            cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa         >  1:590527/1‑100 (MQ=255)
                                                                                            cGACAGAGTAATCTGTCGCCTTTTTTATTTGATTGCTTTCTGGTAGATACCGCCGCAAATATATACTGAATAAAACGGCTAAAAGACGAAACATTATCaa         >  1:895716/1‑100 (MQ=255)
                                                                                             gACAGAGTAATCTGTCGCCTTTTTTCTTTGTTTTTTTTCTGCTAGATTCCGCCACATATTTATCCCGAATGAAACAGCTAAAAGACGAAcctt               >  1:648651/1‑91 (MQ=255)
                                                                                             gACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaaa        >  1:901634/1‑100 (MQ=255)
                                                                                             gACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaaa        >  1:1635069/1‑100 (MQ=255)
                                                                                                agagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACca     >  2:2006784/1‑100 (MQ=255)
                                                                                                 gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcat    >  2:1027779/1‑100 (MQ=255)
                                                                                                   gTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcatca  >  1:1112396/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCATCA  >  NC_000913/4051160‑4051358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: