Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,488,027 A→G 75.9% D237G (GAC→GGC)  lptG → lipopolysaccharide export ABC permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,488,0270AG75.9% 255.5 / 73.3 136D237G (GAC→GGC) lptGlipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand):  ref base A (17/16);  new base G (53/50);  total (70/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCG  >  NC_000913/4487928‑4488122
                                                                                                   |                                                                                               
ggCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGa                                                                                                 >  1:814693/1‑100 (MQ=255)
ggCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGa                                                                                                 >  1:1613625/1‑100 (MQ=255)
      tGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACt                                                                                           >  1:214171/1‑100 (MQ=255)
       gTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTg                                                                                          >  1:1507048/1‑100 (MQ=255)
       gTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTg                                                                                          >  1:1611682/1‑100 (MQ=255)
             ngTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    >  1:2346306/2‑100 (MQ=255)
             ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    >  2:586413/1‑100 (MQ=255)
             ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    >  1:1354289/1‑100 (MQ=255)
             ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    >  2:1292769/1‑100 (MQ=255)
             ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    >  2:56522/1‑100 (MQ=255)
             ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    >  1:2817237/1‑100 (MQ=255)
             ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                    <  2:392016/100‑1 (MQ=255)
              gTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtgg                                                                                   >  1:2373272/1‑100 (MQ=255)
              gTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCgtgg                                                                                   <  2:2253436/100‑1 (MQ=255)
               ttGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtggt                                                                                  <  2:1421613/100‑1 (MQ=255)
                tgatgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCgtggtg                                                                                 <  2:251338/100‑1 (MQ=255)
                  atgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgc                                                                               >  2:1326392/1‑100 (MQ=255)
                   tgaATCTGATCTGACCAATCCGAAACAGCTTACCGGTTCGCAGACGTTGAGCGGGACCGGGCAAACCAACCTCACGCCGGGCAAACTAGGCgtggtcg                                                                                >  1:898412/1‑96 (MQ=255)
                   tgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCCGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgcg                                                                              >  1:2557064/1‑100 (MQ=255)
                    gaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgcgc                                                                             >  1:2875732/1‑100 (MQ=255)
                     aaTCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCt                                                                            <  1:748441/100‑1 (MQ=255)
                      atctgatctgACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGTACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTg                                                                           <  1:2545145/100‑1 (MQ=255)
                         tgatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAc                                                                        >  1:2806993/1‑100 (MQ=255)
                         tgatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAc                                                                        >  2:2340793/1‑100 (MQ=255)
                          gatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAcc                                                                       >  1:1506482/1‑100 (MQ=255)
                             ctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCgg                                                                    >  2:960225/1‑100 (MQ=255)
                              tgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGa                                                                   <  2:2373283/100‑1 (MQ=255)
                              tgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGa                                                                   >  1:423600/1‑100 (MQ=255)
                               gaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCGGGAt                                                                  >  2:870589/1‑100 (MQ=255)
                                aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                 <  2:2383020/100‑1 (MQ=255)
                                aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                 <  2:2604448/100‑1 (MQ=255)
                                aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                 >  1:2562199/1‑100 (MQ=255)
                                aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                 <  1:1536991/100‑1 (MQ=255)
                                 ccAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGc                                                                >  1:2293700/1‑100 (MQ=255)
                                 ccAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGCCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGc                                                                >  1:76635/1‑100 (MQ=255)
                                  cAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCa                                                               >  1:1380103/1‑100 (MQ=255)
                                   aaTCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCAc                                                              >  2:2064421/1‑100 (MQ=255)
                                      ccGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActct                                                           >  2:2726999/1‑100 (MQ=255)
                                       cGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctc                                                          <  2:470681/100‑1 (MQ=255)
                                       cGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctc                                                          >  2:84741/1‑100 (MQ=255)
                                        gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                         <  2:1354291/100‑1 (MQ=255)
                                        gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                         >  2:493950/1‑100 (MQ=255)
                                        gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                         >  1:144141/1‑100 (MQ=255)
                                        gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                         <  2:22326/100‑1 (MQ=255)
                                            cAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATc                                                      <  1:1058550/99‑1 (MQ=255)
                                             aGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCGGGATGCACTCTCTATCAg                                                    <  1:870587/100‑1 (MQ=255)
                                             aGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAg                                                    <  2:484174/100‑1 (MQ=255)
                                              gATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGc                                                   <  1:1098819/100‑1 (MQ=255)
                                              gATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGc                                                   >  2:1845185/1‑100 (MQ=255)
                                               aTTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCg                                                  <  2:1141566/100‑1 (MQ=255)
                                                   ccGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGttt                                              >  2:1254769/1‑100 (MQ=255)
                                                   ccGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGttt                                              >  2:1644762/1‑100 (MQ=255)
                                                    cGGTTCGCAGACGGTGAGCGGCACCTGGAAACCCAACCTCCCGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTg                                             <  2:858090/100‑1 (MQ=255)
                                                     ggTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGc                                            >  1:1325535/1‑100 (MQ=255)
                                                     ggTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGc                                            <  1:650781/100‑1 (MQ=255)
                                                     ggTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGc                                            <  1:2811728/100‑1 (MQ=255)
                                                     ggTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGc                                            >  1:2742518/1‑100 (MQ=255)
                                                       ttCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGcac                                          <  1:907252/100‑1 (MQ=255)
                                                         cGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGcaca                                         <  1:2033030/99‑1 (MQ=255)
                                                         cGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGcaca                                         >  2:277159/1‑99 (MQ=255)
                                                          gCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAAc                                       <  2:551877/100‑1 (MQ=255)
                                                          gCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAAc                                       <  1:784778/100‑1 (MQ=255)
                                                          gCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAAc                                       <  2:1611686/100‑1 (MQ=255)
                                                          gCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAAc                                       >  1:790622/1‑100 (MQ=255)
                                                          gCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAAc                                       >  2:920165/1‑100 (MQ=255)
                                                          gCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAAc                                       <  2:1301208/100‑1 (MQ=255)
                                                            aGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTa                                     <  2:1365634/100‑1 (MQ=255)
                                                              aCGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAtg                                   <  1:1263067/100‑1 (MQ=255)
                                                              aCGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAtg                                   <  1:557799/100‑1 (MQ=255)
                                                                     gCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTa                            <  2:1406558/100‑1 (MQ=255)
                                                                          aCCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGa                       <  1:1673412/100‑1 (MQ=255)
                                                                           ccTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGaa                      <  2:1983081/100‑1 (MQ=255)
                                                                           ccTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGaa                      >  2:405804/1‑100 (MQ=255)
                                                                            cTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                     <  1:663146/100‑1 (MQ=255)
                                                                            cTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                     >  2:1068186/1‑100 (MQ=255)
                                                                            cTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                     >  2:2752137/1‑100 (MQ=255)
                                                                             tGGAAAACCAACCTCACGCCGGGCAAACTGGTCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGt                    <  2:1980279/100‑1 (MQ=255)
                                                                             tGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGt                    >  2:2315047/1‑100 (MQ=255)
                                                                             tGGAAAACCAACATCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACTCGGAGGCACACTATATCAGCGGGATGCACAACTATGTGAAGGATATGAAg                     >  1:2691261/1‑99 (MQ=255)
                                                                              ggAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTc                   <  1:2307939/100‑1 (MQ=255)
                                                                               gAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCg                  >  1:2115679/1‑100 (MQ=255)
                                                                               gAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCg                  <  1:2197028/100‑1 (MQ=255)
                                                                                 aaaCCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCAATCTCTATCAGCGGGTTGCACAACTATGTGAAGTATCTGAAGTCGAg                >  1:2078071/1‑100 (MQ=255)
                                                                                 aaaCCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAg                <  2:2453610/100‑1 (MQ=255)
                                                                                  aaccaaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGc               <  1:2105000/100‑1 (MQ=255)
                                                                                   accaaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCg              >  2:1842077/1‑100 (MQ=255)
                                                                                   accaaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCg              <  1:2905288/100‑1 (MQ=255)
                                                                                    ccaaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCgg             <  2:163334/100‑1 (MQ=255)
                                                                                    ccaaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCgg             >  1:2289121/1‑100 (MQ=255)
                                                                                    ccaaccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACCCTCTATCAGCGGTTTGCACAACTCTGTGAAGTATCTGAAGTCGAg                >  1:1648691/1‑97 (MQ=255)
                                                                                       accTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCa          >  1:1399069/1‑100 (MQ=255)
                                                                                        ccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg         >  2:626603/1‑100 (MQ=255)
                                                                                        ccTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg         >  2:626731/1‑100 (MQ=255)
                                                                                         cTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGTACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg         <  1:1896547/99‑1 (MQ=255)
                                                                                          tCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGa       >  2:2331425/1‑100 (MQ=255)
                                                                                          tCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGGAGTATCTGACGTCGAGCGGTGAgca       >  1:2766240/1‑98 (MQ=255)
                                                                                             cGCCGGGCAAACTGGTGTTGGTGGCGCTGGTCTCGGATGCACTCTCTATCCGCGGTTTGAACATCTCTGTTAAGTTTCTGAAGt                    <  1:2004455/84‑1 (MQ=255)
                                                                                             cGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGc    >  1:1222020/1‑100 (MQ=255)
                                                                                              gCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGcc   >  2:1883069/1‑100 (MQ=255)
                                                                                               ccGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCg  <  1:2893915/100‑1 (MQ=255)
                                                                                                   |                                                                                               
GGCGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCG  >  NC_000913/4487928‑4488122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑