| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,143,641 | G→A | 100% | H243Y (CAT→TAT) | rne ← | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,143,641 | 0 | G | A | 93.7% | 232.2 / 3.3 | 78 | H243Y (CAT→TAT) | rne | endoribonuclease; RNA‑binding protein;RNA degradosome binding protein |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (36/37); total (38/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTG > NC_000913/1143544‑1143735 | aTCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACt < 1:1379064/100‑1 (MQ=255)aTCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACt < 2:2066345/100‑1 (MQ=255) tCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTg < 1:2777225/100‑1 (MQ=255) tCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTg < 2:1715072/100‑1 (MQ=255) cTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGa > 1:1190376/1‑100 (MQ=255) cTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGa > 2:1088615/1‑100 (MQ=255) tgtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGAc > 2:2565952/1‑100 (MQ=255) tcatcaggATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg < 2:647016/93‑1 (MQ=255) gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg > 1:1777707/1‑100 (MQ=255) gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg > 1:2213187/1‑100 (MQ=255) gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg < 2:2418292/100‑1 (MQ=255) gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg > 1:973200/1‑100 (MQ=255) aCTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGc < 1:1589309/100‑1 (MQ=255) cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc < 2:1795808/100‑1 (MQ=255) cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc > 2:2169515/1‑100 (MQ=255) tCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACACTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCa < 1:1862135/100‑1 (MQ=255) cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg < 1:470407/100‑1 (MQ=255) gATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg > 2:1541444/1‑99 (MQ=255) tGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGa < 2:2615987/100‑1 (MQ=255) tGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTAATTTTGTTGATGGAGTCAGGGCGGCCTAATGCAGCGAGCTACTGACGTg > 1:843115/1‑91 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg > 1:2453055/1‑100 (MQ=255) ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCAAg < 2:2409660/100‑1 (MQ=255) gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc < 2:912860/100‑1 (MQ=255) gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc > 2:785728/1‑100 (MQ=255) gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc < 1:1641836/100‑1 (MQ=255) tAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCa > 1:1902195/1‑100 (MQ=255) aGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCAc < 1:428139/100‑1 (MQ=255) gTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACt > 1:1688883/1‑100 (MQ=255) ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt > 1:1485719/1‑100 (MQ=255) cTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCg > 1:1780107/1‑100 (MQ=255) aaCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt < 1:29465/100‑1 (MQ=255) aaCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGt < 1:617531/100‑1 (MQ=255) aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGtt < 1:2267071/100‑1 (MQ=255) cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa < 2:1941371/100‑1 (MQ=255) gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc < 2:1251425/100‑1 (MQ=255) gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa < 2:2530173/100‑1 (MQ=255) gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa < 2:2499672/100‑1 (MQ=255) gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa < 1:1924631/100‑1 (MQ=255) aTCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaa > 1:1068696/1‑100 (MQ=255) tctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAg < 1:258154/100‑1 (MQ=255) tcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGa > 1:1339488/1‑100 (MQ=255) cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt > 2:1576833/1‑100 (MQ=255) tgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGcc > 2:116291/1‑100 (MQ=255) gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg < 2:2384152/100‑1 (MQ=255) aCAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa < 1:549840/99‑1 (MQ=255) gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc > 1:975170/1‑100 (MQ=255) tttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTcc < 2:2213195/100‑1 (MQ=255) tGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGTTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTg < 1:1855427/100‑1 (MQ=255) gATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa > 1:2602595/1‑100 (MQ=255) gATTTGGCTGCTGAAAGCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa < 1:440045/100‑1 (MQ=255) tttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgt < 1:1879474/100‑1 (MQ=255) ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgta < 2:552311/100‑1 (MQ=255) gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag < 2:2570750/100‑1 (MQ=255) gctgctGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaag > 2:2851619/1‑100 (MQ=255) ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt > 2:2640908/1‑100 (MQ=255) tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta > 1:2896551/1‑100 (MQ=255) tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCCATGTCCTGACgtaagta > 1:1358698/1‑100 (MQ=255) ctgAAATCCGGGCGACCTAAGGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt > 1:1772343/1‑100 (MQ=255) aaaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgc > 2:2438589/1‑100 (MQ=255) aaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgcg < 2:1032752/100‑1 (MQ=255) aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg > 1:1453305/1‑100 (MQ=255) aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg > 2:2410398/1‑100 (MQ=255) ccGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa < 2:319991/100‑1 (MQ=255) cGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAAt > 2:2872727/1‑100 (MQ=255) gggCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATg < 1:2676066/100‑1 (MQ=255) ggCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGCGTTCTCGATCCGGACTTCGCCGATGTGCTGACGTAAGTATTcg > 1:2418489/1‑92 (MQ=255) gCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg > 2:1753393/1‑100 (MQ=255) ccTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGaa < 2:477734/100‑1 (MQ=255) tAATGCAGAGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg > 1:2558363/1‑100 (MQ=255) caTGGAGCGATTTACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGa < 1:2697339/99‑1 (MQ=255) aaTGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGa > 1:1758732/1‑100 (MQ=255) tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt < 1:1890753/100‑1 (MQ=255) tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt > 1:742606/1‑100 (MQ=255) gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa < 1:2748258/100‑1 (MQ=255) cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc > 1:1740120/1‑100 (MQ=255) aGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACg < 2:1772349/100‑1 (MQ=255) gCGATATGCTGAAGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGACTTCGCGGATGTCCTGACGTACGTAATCGCGGAATGCGCGAACGACTAc > 1:591924/1‑98 (MQ=255) gATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTg > 2:1568070/1‑100 (MQ=255) | ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTG > NC_000913/1143544‑1143735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |