Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,242 A→T 19.1% intergenic (+335/+37) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,2420AT19.1% 251.8 / 49.2 109intergenic (+335/+37)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (46/42);  new base T (9/12);  total (55/54)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 110 total aligned reads displayed.

AAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCA  >  NC_000913/1224143‑1224339
                                                                                                   |                                                                                                 
aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                   >  2:2844123/1‑100 (MQ=255)
 aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAc                                                                                                  >  2:516278/1‑100 (MQ=255)
  aGGGGGAGGTGGAAATAGCAATGAGGAGTCTCAGCAAGAATACTCGCCGCGTTACCACAACGTGGATGAGAGGAATGAAAAACTCAAGGCAGAGATAAct                                                                                                 <  1:1748128/100‑1 (MQ=255)
   gggggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc                                                                                                >  2:2368823/1‑100 (MQ=255)
      ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGc                                                                                             <  1:2835608/100‑1 (MQ=255)
      ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                             >  2:2823314/1‑100 (MQ=255)
        aGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCt                                                                                           <  2:104210/100‑1 (MQ=255)
         ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                          >  2:2064495/1‑100 (MQ=255)
         ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                          >  2:2828404/1‑100 (MQ=255)
         ggTGGAAATAGCAATGAGGAGTATCAGCAAGAAGACTCGCCGCTTTACCACAACGTTGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCt                                                                                           <  1:61750/99‑1 (MQ=255)
         ggTGGAAATAGCAACGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                          <  1:814056/100‑1 (MQ=255)
          gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCtt                                                                                          <  1:2047979/99‑1 (MQ=255)
          gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                         <  2:1181303/100‑1 (MQ=255)
          gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                         <  1:1477306/100‑1 (MQ=255)
          gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                         <  1:1113324/100‑1 (MQ=255)
           tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGa                                                                                        <  2:343802/100‑1 (MQ=255)
           tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGa                                                                                        <  2:146862/100‑1 (MQ=255)
            ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                       >  2:1362091/1‑100 (MQ=255)
               aaTAGCAATGAGTAGTATCAGCAAGAAGACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagtt                                                                                     <  1:230379/99‑4 (MQ=38)
               aaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagttt                                                                                    <  1:1252575/100‑5 (MQ=38)
                aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATa                                                                                   >  2:847528/1‑100 (MQ=255)
                 tAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaa                                                                                  >  2:2267330/1‑100 (MQ=255)
                    cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg                                                                               <  1:1155618/100‑1 (MQ=255)
                         aggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtt                                                                          >  1:925003/1‑100 (MQ=255)
                          ggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                         >  2:2129935/1‑100 (MQ=255)
                          ggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                         >  1:1022512/1‑100 (MQ=255)
                           gagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtttt                                                                        <  2:2029272/100‑1 (MQ=255)
                             gTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTAc                                                                      >  2:1030818/1‑100 (MQ=255)
                               aTCAGCAAGAATACTGGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa                                                                     >  1:1371539/1‑99 (MQ=255)
                               aTCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg                                                                    >  2:2676701/1‑100 (MQ=255)
                                 cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                  >  1:913924/1‑100 (MQ=255)
                                 cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                  <  1:1458537/100‑1 (MQ=255)
                                  aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg                                                                 >  1:1353865/1‑100 (MQ=255)
                                  aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg                                                                 >  1:841831/1‑100 (MQ=255)
                                  aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                  >  2:516000/1‑99 (MQ=255)
                                   gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg                                                                >  1:940296/1‑100 (MQ=255)
                                    cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc                                                               <  1:2828394/100‑1 (MQ=255)
                                     aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                              <  2:1372366/100‑1 (MQ=255)
                                      aGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt                                                             >  2:1012456/1‑100 (MQ=255)
                                      aGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt                                                             >  1:1647636/1‑100 (MQ=255)
                                       gAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                            <  1:129498/100‑1 (MQ=255)
                                        aaTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt                                                           <  2:1800451/100‑1 (MQ=255)
                                         aTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAGACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                          <  2:1840182/100‑1 (MQ=255)
                                            cTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                        >  2:19356/1‑99 (MQ=255)
                                              cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                     <  1:1362089/100‑1 (MQ=255)
                                              cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                     >  1:1700729/1‑100 (MQ=255)
                                                   cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                <  1:1996179/100‑1 (MQ=255)
                                                   cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                >  2:315198/1‑100 (MQ=255)
                                                    tttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttct                                               <  2:1099208/100‑1 (MQ=255)
                                                     ttACCACAACGTTGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                <  1:2329273/98‑1 (MQ=255)
                                                      tACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGc                                             >  1:2606295/1‑100 (MQ=255)
                                                        ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                           >  1:1073297/1‑100 (MQ=255)
                                                         cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc                                          <  2:809273/100‑1 (MQ=255)
                                                         cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc                                          <  2:429204/100‑1 (MQ=255)
                                                          acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                         >  1:1065932/1‑100 (MQ=255)
                                                          acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                         <  1:1747714/100‑1 (MQ=255)
                                                           caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg                                        >  1:395800/1‑100 (MQ=255)
                                                            aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                       <  2:1925089/100‑1 (MQ=255)
                                                            aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                       <  1:505616/100‑1 (MQ=255)
                                                            aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                       <  2:2691607/100‑1 (MQ=255)
                                                             aCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt                                      <  2:2537815/100‑1 (MQ=255)
                                                             aCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt                                      <  1:1030816/100‑1 (MQ=255)
                                                             aCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt                                      >  2:1083067/1‑100 (MQ=255)
                                                                tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                   <  2:2621737/100‑1 (MQ=255)
                                                                tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                   >  2:162137/1‑100 (MQ=255)
                                                                    tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCa                               >  2:2035786/1‑100 (MQ=255)
                                                                      agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACg                             >  2:2088171/1‑100 (MQ=255)
                                                                        tctgATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGtt                           <  1:162425/97‑1 (MQ=255)
                                                                         gggATGAGAAACTCAATGCAGAGATATCTTTGCTTGGAATATAAACGCTGTTTTGCAGTGGTCTTATTTCAGCTCTTCTGCTTCCGGCAAGCGCACGTTc                          <  1:2673336/100‑1 (MQ=255)
                                                                         gggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc                          >  1:1358819/1‑100 (MQ=255)
                                                                         gggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc                          >  2:2783780/1‑100 (MQ=255)
                                                                         gggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc                          <  1:2676691/100‑1 (MQ=255)
                                                                          ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCa                         <  2:1651969/100‑1 (MQ=255)
                                                                           gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGttaga                        >  2:162426/1‑97 (MQ=255)
                                                                           gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                        <  2:1673055/100‑1 (MQ=255)
                                                                           gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                        >  1:920798/1‑100 (MQ=255)
                                                                           gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAAAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCa                         >  1:703605/1‑99 (MQ=255)
                                                                             tGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCt                      <  1:2129928/100‑1 (MQ=255)
                                                                              gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                     >  1:1385757/1‑100 (MQ=255)
                                                                               aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCa                    <  2:581582/100‑1 (MQ=255)
                                                                               aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCa                    <  2:2900506/100‑1 (MQ=255)
                                                                                aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATCAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCaa                   <  1:714954/100‑1 (MQ=255)
                                                                                  aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa                 >  2:2059170/1‑100 (MQ=255)
                                                                                  aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa                 >  1:487215/1‑100 (MQ=255)
                                                                                   cttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGaa                >  1:2837384/3‑100 (MQ=255)
                                                                                   cttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGaa                >  1:823353/3‑100 (MQ=255)
                                                                                    cTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt               <  2:920800/100‑1 (MQ=255)
                                                                                     tCAAGGCAGACATAAGTCTTCTTTGAAGATAAATGCGCTTTTACAGCGCGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGATCAAGAAt               <  1:1088876/99‑1 (MQ=255)
                                                                                      cAAGGCAGAGATAACTCTGCCTTGAAGATAAAGGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg             <  1:1359186/100‑1 (MQ=255)
                                                                                       aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  1:1531033/1‑100 (MQ=255)
                                                                                       aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  2:1331055/1‑100 (MQ=255)
                                                                                       aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  1:2839534/1‑100 (MQ=255)
                                                                                       aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  1:1344063/1‑100 (MQ=255)
                                                                                       aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  2:697881/1‑100 (MQ=255)
                                                                                       aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCATCTGCTTCCGGTAAGGTCACGTACAGCTCATGAATAg             >  1:303128/1‑99 (MQ=255)
                                                                                        aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa           >  1:417009/1‑100 (MQ=255)
                                                                                        aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa           <  2:835370/100‑1 (MQ=255)
                                                                                         ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa          >  1:1035950/1‑100 (MQ=255)
                                                                                             gagaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATc      >  1:2142270/1‑100 (MQ=255)
                                                                                                 tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCa  <  1:1647045/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
AAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCA  >  NC_000913/1224143‑1224339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: