Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,033 A→G 18.5% L105P (CTG→CCG)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0330AG18.5% 181.2 / 33.6 87L105P (CTG→CCG) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base G (8/8);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCT  >  NC_000913/1343934‑1344129
                                                                                                   |                                                                                                
acGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCa                                                                                                  <  1:2571976/100‑1 (MQ=255)
 cGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTTGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCag                                                                                                 <  2:530323/100‑1 (MQ=255)
 cGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTTCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCag                                                                                                 >  2:2550513/1‑100 (MQ=255)
    cACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGt                                                                                              <  1:1723549/100‑1 (MQ=255)
     aCCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTc                                                                                             <  1:1398941/100‑1 (MQ=255)
          gCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGc                                                                                        <  1:1320772/100‑1 (MQ=255)
           cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                       <  1:2522528/100‑1 (MQ=255)
           cAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCg                                                                                       <  2:1611420/100‑1 (MQ=255)
            aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                      >  2:767846/1‑100 (MQ=255)
            aaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                      >  2:195010/1‑100 (MQ=255)
             aaaTAACTTCACAAGGCGCGTCTTTCAGCACATGCGCGATGTAGCTGCTGCCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCCGCGTCCGGGCGAg                                                                                     <  1:2032168/100‑1 (MQ=255)
              aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAgc                                                                                    <  1:723403/100‑2 (MQ=255)
               aTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTa                                                                                   <  1:453229/100‑1 (MQ=255)
               aTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTa                                                                                   >  1:1606880/1‑100 (MQ=255)
                 aaCTTCACAAGGCGCGTCTTTCAGTAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAga                                                                                 <  1:1344255/100‑2 (MQ=38)
                 aaCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAga                                                                                 <  2:1713544/100‑2 (MQ=38)
                  aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                >  2:2702748/1‑100 (MQ=255)
                  aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAggg                                                                                <  1:2272538/100‑1 (MQ=255)
                  aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgg                                                                                 >  1:452150/1‑99 (MQ=255)
                  aCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTACTCGCCCGGACTCTGGGCGAGCAgaa                                                                                <  1:2706589/100‑3 (MQ=38)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgcagaag                                                                               <  2:1806889/100‑7 (MQ=38)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgcagaag                                                                               <  1:846065/100‑7 (MQ=38)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAgcagaag                                                                               <  2:485335/100‑7 (MQ=38)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                               >  2:479261/1‑100 (MQ=255)
                   cTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                               >  2:1823409/1‑100 (MQ=255)
                    ttCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCa                                                                              <  1:2626739/100‑1 (MQ=255)
                         aaGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgc                                                                          <  1:2742662/99‑1 (MQ=255)
                           ggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgc                                                                       <  2:771862/100‑1 (MQ=255)
                            gcgcGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTCCTTCTGCTCGTCCAGAGTCCGGGCGAGTTGGGCATtgctgct                                                                      <  2:1962746/100‑1 (MQ=255)
                              gcgTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctgc                                                                    <  2:1568927/100‑1 (MQ=255)
                                gTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGcc                                                                   >  1:418482/1‑99 (MQ=255)
                                  cTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtt                                                                >  2:103040/1‑100 (MQ=255)
                                  cTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtt                                                                >  2:339188/1‑100 (MQ=255)
                                    ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTTTTTTTTTTTTCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttt                                                               >  1:281885/1‑99 (MQ=255)
                                    ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                              >  1:2262861/1‑100 (MQ=255)
                                    ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGAGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttct                                                              >  1:1268413/1‑98 (MQ=255)
                                      cAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGATTAGTGCATTGCTGCTGCCATTTTCg                                                            <  1:822618/100‑1 (MQ=255)
                                       aGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGa                                                           >  2:1715793/1‑100 (MQ=255)
                                        gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                          <  2:1786092/100‑1 (MQ=255)
                                        gCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGAt                                                          >  1:2005442/1‑100 (MQ=255)
                                         cAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATa                                                         <  2:2512859/100‑1 (MQ=255)
                                              gcgcGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTGTTCTGCTCGCCCAGAGTCCGTGCTAGTAGTTCATTGCTGCTGCCATTTCCGATAAAg                                                      >  1:1084531/1‑98 (MQ=255)
                                                    tGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTCGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                              >  1:1965301/1‑100 (MQ=255)
                                                    tGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                              >  2:2888222/1‑100 (MQ=255)
                                                      tAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGCGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGc                                            <  2:1447515/100‑1 (MQ=255)
                                                      tAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGTGTAGGGCATTTCTGCTGCCATTTTCGATAAAGATGGTTTcac                                            <  2:1965309/100‑3 (MQ=255)
                                                      tAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGc                                            >  2:638477/1‑100 (MQ=255)
                                                       aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                           <  2:2382656/100‑1 (MQ=255)
                                                       aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                           >  1:483851/1‑100 (MQ=255)
                                                          tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTGGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCgg                                        >  2:2049271/1‑100 (MQ=255)
                                                          tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCgg                                        >  2:1067643/1‑100 (MQ=255)
                                                            ctgACCGTGATGATAGTGACGTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                      <  1:1973744/100‑1 (MQ=255)
                                                            ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                      >  1:1652974/1‑100 (MQ=255)
                                                             tgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                     <  1:978020/100‑1 (MQ=255)
                                                             tgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                     >  1:1154564/1‑100 (MQ=255)
                                                             gatctgTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTg                                     <  1:1002983/95‑1 (MQ=255)
                                                              gACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGa                                    >  2:1191620/1‑100 (MQ=255)
                                                               aCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa                                   <  2:1680155/100‑1 (MQ=255)
                                                               aCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGaa                                   <  2:2490039/100‑1 (MQ=255)
                                                                ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                  >  1:2188516/1‑100 (MQ=255)
                                                                ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                  <  1:772529/100‑1 (MQ=255)
                                                                ccGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAAc                                  <  2:1865074/100‑1 (MQ=255)
                                                                  gTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGagatc                                >  2:1002985/1‑96 (MQ=255)
                                                                     atgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAACGATGGTTTCGCCCGGTTGAACCAGc                              <  1:578503/99‑1 (MQ=255)
                                                                       gatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAc                           <  1:1839088/100‑1 (MQ=255)
                                                                       gatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAc                           <  2:1154566/100‑1 (MQ=255)
                                                                         tAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgc                         <  1:1665921/100‑1 (MQ=255)
                                                                          aGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcgcg                        <  2:1189190/100‑1 (MQ=255)
                                                                           gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                       >  2:1864488/1‑100 (MQ=255)
                                                                           gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCgg                       >  1:2157555/1‑100 (MQ=255)
                                                                             gACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCa                     >  1:1864799/1‑100 (MQ=255)
                                                                               cATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCaaa                   >  2:1007833/1‑100 (MQ=255)
                                                                                    atgccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCgg              >  2:1362832/9‑100 (MQ=37)
                                                                                      gccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg            >  2:1226752/7‑100 (MQ=38)
                                                                                      cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCg            <  1:256352/100‑1 (MQ=255)
                                                                                        cctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGCACCAGCGACGAGGCAAACTCGGCGa           >  1:2462306/5‑99 (MQ=38)
                                                                                         ctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt         >  2:404468/4‑100 (MQ=255)
                                                                                          tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGt         >  2:2266924/3‑99 (MQ=255)
                                                                                          tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGACAAACTCGGCGAGtt        >  2:456753/3‑100 (MQ=255)
                                                                                          tGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGtt        >  1:502506/1‑100 (MQ=255)
                                                                                           ncTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc       >  2:133075/2‑100 (MQ=255)
                                                                                           gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGCTc       >  1:628041/1‑100 (MQ=255)
                                                                                           acTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTc       >  1:2335630/2‑100 (MQ=255)
                                                                                            cTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCa      >  2:354329/1‑100 (MQ=255)
                                                                                               gCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGc   >  1:1248915/1‑100 (MQ=255)
                                                                                                cccAGAGTCCGGGCGAGTAGGGCATTGTTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt  >  2:3868/1‑100 (MQ=255)
                                                                                                cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt  >  1:916365/1‑100 (MQ=255)
                                                                                                cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt  <  1:1111028/100‑1 (MQ=255)
                                                                                                cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt  >  1:912081/1‑100 (MQ=255)
                                                                                                cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTAAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCt  <  2:1691307/100‑1 (MQ=255)
                                                                                                   |                                                                                                
ACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCT  >  NC_000913/1343934‑1344129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: