Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,964 C→T 16.7% intergenic (‑37/+20) ralR ← / ← recT Rac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,9640CT16.7% 278.5 / 43.2 127intergenic (‑37/+20)ralR/recTRac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein
Reads supporting (aligned to +/- strand):  ref base C (57/48);  new base T (9/12);  total (67/60)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

Only 100 of 127 total aligned reads displayed.

GCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCC  >  NC_000913/1413866‑1414062
                                                                                                  |                                                                                                  
gCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCa                                                                                                   <  2:2261338/100‑1 (MQ=255)
 cattaaaccttACTGATGGAAAAACACAAAATGGACATAGTTAAATATTTTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCa                                                                                                   <  1:1889319/90‑1 (MQ=255)
  tttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCAtt                                                                                                 >  2:2058010/1‑100 (MQ=255)
   ttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTTTTATATCTCATAATTTTTCTCATATAAAATATTTCAAGTTGGCGGTGCATTa                                                                                                <  1:389013/100‑1 (MQ=255)
    tCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTac                                                                                               >  1:1832888/1‑100 (MQ=255)
       ccGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACAcc                                                                                            >  1:2016890/1‑100 (MQ=255)
         gTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGc                                                                                          <  2:2668827/100‑1 (MQ=255)
          ttACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGcc                                                                                         <  2:2170634/100‑1 (MQ=255)
           tACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCa                                                                                        <  2:2647966/100‑1 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:782519/100‑3 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:517732/100‑3 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:2814432/100‑3 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  1:1067671/1‑100 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  2:1172156/1‑100 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      <  1:2797711/100‑1 (MQ=255)
             cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  1:1219895/1‑100 (MQ=255)
             cTGATGGACAACCACAAAATGGACATAGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  1:2428047/100‑3 (MQ=255)
             cTGATGGAAAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  1:2228863/100‑3 (MQ=255)
              ttaTGGAAAACCACAAAATGGACATAGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  1:2604939/97‑3 (MQ=255)
              tGATGGACAACCACAAAATTGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaact                                                                                     <  1:1108065/100‑4 (MQ=255)
              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaact                                                                                     <  1:441167/100‑4 (MQ=255)
              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaact                                                                                     <  2:395294/100‑4 (MQ=255)
              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGc                                                                                     >  2:410742/1‑100 (MQ=255)
              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGc                                                                                     <  2:1706988/100‑1 (MQ=255)
              tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCACATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGc                                                                                     <  1:760030/100‑1 (MQ=255)
               gATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCt                                                                                    >  1:331767/1‑100 (MQ=255)
               gATGGACAACAACAAAATGGAAGTGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:2825261/98‑3 (MQ=255)
                  ggACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                  >  1:329338/1‑99 (MQ=255)
                   gACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                  <  1:1878740/98‑1 (MQ=255)
                     cAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatta                                                                              >  2:1514441/1‑100 (MQ=255)
                     cAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatta                                                                              >  2:2680729/1‑100 (MQ=255)
                     cAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCAGTGCATTACACCGCCAGGCTGAatta                                                                              >  1:2275627/1‑100 (MQ=255)
                      aaCCACAAAATTATCATCTTTTAACATTTTCATATCTCATAATTTTTCACACAAAAATTATTTCTCGTTGGCGGTGCATTACACCGTCAGGCTGAat                                                                                <  1:2519515/97‑1 (MQ=255)
                       aCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAAAATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAattatt                                                                            >  1:2046743/1‑100 (MQ=255)
                        ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                           >  1:245430/1‑100 (MQ=255)
                        ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                           >  2:35979/1‑100 (MQ=255)
                        ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                           >  2:947168/1‑100 (MQ=255)
                         cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                          <  1:2655790/100‑1 (MQ=255)
                         cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                          >  2:2875469/1‑100 (MQ=255)
                          acaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACAGCCAGGCTGAATTATTAct                                                                         >  1:1977381/1‑100 (MQ=255)
                            aaaaTGGACATGGTTTAACATTGTCATATATCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGAAGAATTCTTCctct                                                                       >  2:2426252/1‑100 (MQ=255)
                                 ggACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAtt                                                                  >  2:1121758/1‑100 (MQ=255)
                                    cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                               <  2:970898/100‑1 (MQ=255)
                                    cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTCGCGGTACATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                               <  1:2419181/100‑1 (MQ=255)
                                      tGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa                                                             <  1:589303/100‑1 (MQ=255)
                                       ggTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                            >  2:1403505/1‑100 (MQ=255)
                                       ggTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGACAGGCTGAATTATTCCTCTGAATTATCGAt                                                            >  1:2744279/1‑100 (MQ=255)
                                        gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAtt                                                           >  1:2050214/1‑100 (MQ=255)
                                          ttAACATTGTCATATCTCATAATTTTTCTTTTATAATATATTTCAATTTGGCGGTAGATTACATCGACAGGCTGAATTATTTCTCTGAATTagctat                                                            >  1:2336320/1‑92 (MQ=255)
                                          ttAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTac                                                         >  2:1851421/1‑100 (MQ=255)
                                           tAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAATTTGGCGGTGCATTACACCGCAAGGCTGAATTATTCCTCTGAATTATCGATTaca                                                        >  1:1847683/1‑100 (MQ=255)
                                            aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                       >  1:995397/1‑100 (MQ=255)
                                            aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                       <  2:244928/100‑1 (MQ=255)
                                            aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTCGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCCATTacac                                                       >  1:3321/1‑100 (MQ=255)
                                              cATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                      >  1:456965/1‑99 (MQ=255)
                                              cATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTg                                                     <  1:350934/100‑1 (MQ=255)
                                                 tGTCATAGCTCATAATTTTTCTCATAACAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTAt                                                  <  1:1436073/100‑1 (MQ=255)
                                                  gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTAtt                                                 >  2:1917632/1‑100 (MQ=255)
                                                   tCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTc                                                >  2:1756161/1‑100 (MQ=255)
                                                        tctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCgg                                           >  2:420474/1‑100 (MQ=255)
                                                        tctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCgg                                           <  2:1720755/100‑1 (MQ=255)
                                                         ctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGt                                          <  2:268462/100‑1 (MQ=255)
                                                         ctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGt                                          >  2:1908483/1‑100 (MQ=255)
                                                          tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                         <  2:2293521/100‑1 (MQ=255)
                                                          tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                         >  1:705/1‑100 (MQ=255)
                                                           ctcttccgatctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTa                                        <  2:1049112/91‑1 (MQ=255)
                                                           cATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCTCCGGTTa                                        >  1:250710/1‑100 (MQ=255)
                                                                   ttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACagag                                <  1:747041/100‑1 (MQ=255)
                                                                    tctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTCCACTGTATTCCCCGGTTAATACTGAgg                               >  1:1428859/1‑100 (MQ=255)
                                                                    tctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAgg                               <  2:531681/100‑1 (MQ=255)
                                                                      tcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGaa                             >  1:1019198/1‑100 (MQ=255)
                                                                       cATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAAt                            >  1:819891/1‑100 (MQ=255)
                                                                       cATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAAt                            >  2:1046366/1‑100 (MQ=255)
                                                                        aTAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATc                           >  1:214172/1‑100 (MQ=255)
                                                                         tAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCt                          <  1:407276/100‑1 (MQ=255)
                                                                          aaaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                         >  1:2246162/1‑100 (MQ=255)
                                                                           aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGc                        <  1:2879943/100‑1 (MQ=255)
                                                                            aaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCa                       <  2:1764918/100‑1 (MQ=255)
                                                                            aaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCa                       <  2:148354/100‑1 (MQ=255)
                                                                             aaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                      <  2:424281/100‑1 (MQ=255)
                                                                                  tttCGAGTTGGCGGTGCATTTCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTACCCGGTTAATACAGAGGAATCTGCAGGAttg                 >  1:1278443/1‑98 (MQ=255)
                                                                                  tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCg                 >  2:2209167/1‑100 (MQ=255)
                                                                                  tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCg                 <  2:1599924/100‑1 (MQ=255)
                                                                                  tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCg                 <  2:1159724/100‑1 (MQ=255)
                                                                                    tCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGAt               <  2:1810533/100‑1 (MQ=255)
                                                                                      agcctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTGTCACTTACACTGTATTCCCCGGTTCATCCAGAGCAATCTGCAGGATCGAtt              >  1:470589/5‑99 (MQ=25)
                                                                                      aaGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTg             >  2:553322/1‑100 (MQ=255)
                                                                                       gcctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt            >  2:128464/4‑100 (MQ=255)
                                                                                       aGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt            >  1:177514/1‑100 (MQ=255)
                                                                                         ctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCa          >  2:1983743/2‑100 (MQ=255)
                                                                                          tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTCCACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCa          >  1:1107704/1‑99 (MQ=255)
                                                                                          tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTc           >  1:2357288/1‑98 (MQ=255)
                                                                                          tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg         >  2:2122167/1‑100 (MQ=255)
                                                                                          tGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg         <  2:1242636/100‑1 (MQ=255)
                                                                                           ggCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt        >  1:1669139/1‑100 (MQ=255)
                                                                                           ggCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt        <  2:2204246/100‑1 (MQ=255)
                                                                                              ggTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGt     <  2:1143279/100‑1 (MQ=255)
                                                                                                 gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  >  1:2009201/1‑100 (MQ=255)
                                                                                                 gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  <  2:1309765/100‑1 (MQ=255)
                                                                                                 gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  <  2:240198/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
GCTTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCC  >  NC_000913/1413866‑1414062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: