Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,413,966 T→A 16.9% intergenic (‑39/+18) ralR ← / ← recT Rac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,9660TA16.9% 298.4 / 51.6 124intergenic (‑39/+18)ralR/recTRac prophage; restriction alleviation protein/Rac prophage; recombination and repair protein
Reads supporting (aligned to +/- strand):  ref base T (57/46);  new base A (9/12);  total (66/58)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01

Only 100 of 125 total aligned reads displayed.

TTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCC  >  NC_000913/1413868‑1414062
                                                                                                  |                                                                                                
tttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCAtt                                                                                                 >  2:2058010/1‑100 (MQ=255)
 ttCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTTTTATATCTCATAATTTTTCTCATATAAAATATTTCAAGTTGGCGGTGCATTa                                                                                                <  1:389013/100‑1 (MQ=255)
  tCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTac                                                                                               <  2:702680/100‑1 (MQ=255)
  tCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTac                                                                                               >  1:1832888/1‑100 (MQ=255)
       gTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGc                                                                                          <  2:2668827/100‑1 (MQ=255)
        ttACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGcc                                                                                         <  2:2170634/100‑1 (MQ=255)
         tACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCa                                                                                        <  2:2647966/100‑1 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTATCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  1:2066733/1‑100 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:782519/100‑3 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:517732/100‑3 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:2814432/100‑3 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  1:1067671/1‑100 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  2:1172156/1‑100 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      <  1:2797711/100‑1 (MQ=255)
           cTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAgg                                                                                      >  1:1219895/1‑100 (MQ=255)
           cTGATGGACAACCACAAAATGGACATAGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  1:2428047/100‑3 (MQ=255)
            ttaTGGAAAACCACAAAATGGACATAGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  1:2604939/97‑3 (MQ=255)
            tGATGGACAACCACAAAATTGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaact                                                                                     <  1:1108065/100‑4 (MQ=255)
            tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaact                                                                                     <  1:441167/100‑4 (MQ=255)
            tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaact                                                                                     <  2:395294/100‑4 (MQ=255)
            tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGc                                                                                     >  2:410742/1‑100 (MQ=255)
            tGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCACATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGc                                                                                     <  1:760030/100‑1 (MQ=255)
             gATGGACAACCAGAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCt                                                                                    >  1:435423/1‑100 (MQ=255)
             gATGGACAACAACAAAATGGAAGTGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGTAATGCACCGCCaac                                                                                      <  2:2825261/98‑3 (MQ=255)
               tGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                  >  1:2853838/1‑100 (MQ=255)
                ggACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                  >  1:329338/1‑99 (MQ=255)
                 gACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGa                                                                                  <  1:1878740/98‑1 (MQ=255)
                   cAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAatta                                                                              >  2:1514441/1‑100 (MQ=255)
                   cAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCAGTGCATTACACCGCCAGGCTGAatta                                                                              >  1:2275627/1‑100 (MQ=255)
                    aaCCACAAAATTATCATCTTTTAACATTTTCATATCTCATAATTTTTCACACAAAAATTATTTCTCGTTGGCGGTGCATTACACCGTCAGGCTGAat                                                                                <  1:2519515/97‑1 (MQ=255)
                     aCCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAAAATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAattatt                                                                            >  1:2046743/1‑100 (MQ=255)
                      ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                           >  1:245430/1‑100 (MQ=255)
                      ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                           >  2:35979/1‑100 (MQ=255)
                      ccACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                                                                           >  2:947168/1‑100 (MQ=255)
                       cacaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTcc                                                                          <  1:2655790/100‑1 (MQ=255)
                        acaAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACAGCCAGGCTGAATTATTAct                                                                         >  1:1977381/1‑100 (MQ=255)
                          aaaaTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctct                                                                       >  2:1771706/1‑100 (MQ=255)
                             aTGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGaa                                                                    >  2:350936/1‑100 (MQ=255)
                              tGGCCATGGTTTAACATTGTCCTATCTCATCATCTTTCTCATAAAAAATCTTTCCAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTTaa                                                                    <  1:2426241/99‑1 (MQ=255)
                               ggACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAtt                                                                  >  2:1121758/1‑100 (MQ=255)
                                  cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                               <  2:970898/100‑1 (MQ=255)
                                  cATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTCGCGGTACATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc                                                               <  1:2419181/100‑1 (MQ=255)
                                     ggTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt                                                            >  2:1403505/1‑100 (MQ=255)
                                      gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAtt                                                           >  1:2050214/1‑100 (MQ=255)
                                      gTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAtt                                                           >  1:2291890/1‑100 (MQ=255)
                                        ttAACATTGTCATATCTCATAATTTTTCTTTTATAATATATTTCAATTTGGCGGTAGATTACATCGACAGGCTGAATTATTTCTCTGAATTagctat                                                            >  1:2336320/1‑92 (MQ=255)
                                        ttAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTac                                                         >  2:1851421/1‑100 (MQ=255)
                                         tAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAATTTGGCGGTGCATTACACCGCAAGGCTGAATTATTCCTCTGAATTATCGATTaca                                                        >  1:1847683/1‑100 (MQ=255)
                                          aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                       <  2:244928/100‑1 (MQ=255)
                                          aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTacac                                                       >  1:995397/1‑100 (MQ=255)
                                          aaCATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTCGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCCATTacac                                                       >  1:3321/1‑100 (MQ=255)
                                            cATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt                                                      >  1:456965/1‑99 (MQ=255)
                                            cATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTg                                                     <  1:350934/100‑1 (MQ=255)
                                               tGTCATAGCTCATAATTTTTCTCATAACAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTAt                                                  <  1:1436073/100‑1 (MQ=255)
                                                gTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTAtt                                                 >  2:1917632/1‑100 (MQ=255)
                                                 tCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTc                                                >  2:1756161/1‑100 (MQ=255)
                                                    tatCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTcccc                                             <  2:1936179/100‑1 (MQ=255)
                                                      tctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCgg                                           <  2:1720755/100‑1 (MQ=255)
                                                       ctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGt                                          <  2:268462/100‑1 (MQ=255)
                                                       ctcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGt                                          >  2:1908483/1‑100 (MQ=255)
                                                        tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                         >  1:705/1‑100 (MQ=255)
                                                        tcATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                         <  2:2293521/100‑1 (MQ=255)
                                                         ctcttccgatctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTa                                        <  2:1049112/91‑1 (MQ=255)
                                                           tAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGtt                                         >  1:1999092/1‑97 (MQ=255)
                                                            aaTTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATa                                     <  2:2484838/100‑1 (MQ=255)
                                                             aatgttatcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATAc                                    <  2:421474/93‑1 (MQ=255)
                                                                 ttCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACagag                                <  1:747041/100‑1 (MQ=255)
                                                                  tctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTCCACTGTATTCCCCGGTTAATACTGAgg                               >  1:1428859/1‑100 (MQ=255)
                                                                  tctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAgg                               <  2:531681/100‑1 (MQ=255)
                                                                  tctcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAgg                               <  2:2281534/100‑1 (MQ=255)
                                                                    tcATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGaa                             >  1:1019198/1‑100 (MQ=255)
                                                                     cATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAAt                            >  2:1046366/1‑100 (MQ=255)
                                                                      aTAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATc                           >  1:214172/1‑100 (MQ=255)
                                                                      aTAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATc                           <  2:2187779/100‑1 (MQ=255)
                                                                        aaaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTg                         >  1:2246162/1‑100 (MQ=255)
                                                                         aaaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGc                        <  1:2879943/100‑1 (MQ=255)
                                                                          aaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCa                       <  2:1764918/100‑1 (MQ=255)
                                                                          aaaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCa                       <  2:148354/100‑1 (MQ=255)
                                                                           aaaTATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAg                      <  2:424281/100‑1 (MQ=255)
                                                                                tttCGAGTTGGCGGTGCATTTCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTACCCGGTTAATACAGAGGAATCTGCAGGAttg                 >  1:1278443/1‑98 (MQ=255)
                                                                                tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCg                 >  2:2209167/1‑100 (MQ=255)
                                                                                tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCg                 <  2:1159724/100‑1 (MQ=255)
                                                                                tttCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCg                 <  2:1599924/100‑1 (MQ=255)
                                                                                    agcctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTGTCACTTACACTGTATTCCCCGGTTCATCCAGAGCAATCTGCAGGATCGAtt              >  1:470589/5‑99 (MQ=25)
                                                                                     gcctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt            >  2:128464/4‑100 (MQ=255)
                                                                                     gcctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt            >  1:67998/4‑100 (MQ=255)
                                                                                     aGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGt            >  1:177514/1‑100 (MQ=255)
                                                                                      cctGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTc           >  1:339204/3‑100 (MQ=255)
                                                                                        tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTCCACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCa          >  1:1107704/1‑99 (MQ=255)
                                                                                        tGGCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg         >  2:2122167/1‑100 (MQ=255)
                                                                                        tGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAg         <  2:1242636/100‑1 (MQ=255)
                                                                                         ggCGGTGTAATGCACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGt        >  1:1669139/1‑100 (MQ=255)
                                                                                          gCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTg       <  1:2597139/100‑1 (MQ=255)
                                                                                           cGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTgg      >  1:902692/1‑100 (MQ=255)
                                                                                            ggTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGt     <  2:1143279/100‑1 (MQ=255)
                                                                                             gTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGtt    >  2:2774963/1‑100 (MQ=255)
                                                                                               gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  <  2:1309765/100‑1 (MQ=255)
                                                                                               gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  >  1:2009201/1‑100 (MQ=255)
                                                                                               gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  <  2:1654980/100‑1 (MQ=255)
                                                                                               gCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTcc  <  2:240198/100‑1 (MQ=255)
                                                                                                  |                                                                                                
TTTCACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACTGTATTCCCCGGTTAATACAGAGGAATCTGCAGGATCGATTGTCAGTGGTTCC  >  NC_000913/1413868‑1414062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: