Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,835,622 G→A 100% R36R (CGG→CGA)  ynjA → carboxymuconolactone decarboxylase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,835,6220GA94.7% 347.0 / 5.3 118R36R (CGG→CGA) ynjAcarboxymuconolactone decarboxylase family protein
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base A (47/64);  total (49/69)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CCGCTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAGCGCG  >  NC_000913/1835527‑1835718
                                                                                               |                                                                                                
ccgcTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATcc                                                                                              <  2:2402668/100‑1 (MQ=255)
ccgcTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATcc                                                                                              >  2:1494433/1‑100 (MQ=255)
ccgcTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATcc                                                                                              <  2:815564/100‑1 (MQ=255)
 cgcTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATccc                                                                                             <  2:717131/100‑1 (MQ=255)
  gcTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGGGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCg                                                                                            >  2:2241085/1‑100 (MQ=255)
    ttAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtt                                                                                          <  2:2466379/100‑1 (MQ=255)
    ttAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtt                                                                                          <  1:1433968/100‑1 (MQ=255)
     tAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGttt                                                                                         >  1:2269316/1‑100 (MQ=255)
       gCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttat                                                                                       >  2:140548/1‑100 (MQ=255)
        cAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatc                                                                                      >  2:1058710/1‑100 (MQ=255)
         aaaaTTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCATCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatct                                                                                     <  2:1031607/100‑1 (MQ=255)
          aatttCCTTTAGTTGTACGTCTAGAGTGCAGGCTCCATCGTCGCCTGTAGGGCGAGGTGCTAAGCCCCATTCGCTGGTTGGGGTGAATCCCGtttatctt                                                                                    <  1:195923/97‑1 (MQ=255)
            atgccTTTAATTTTACGTCCACAGGCATGGCTGCATCGTCACCATTACGGCGAAGTACCACACCACCGTCGGGGGTGGGGGCGAAGACCGtttatcgt                                                                                    >  1:431336/4‑96 (MQ=255)
            aTTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatctttt                                                                                  <  2:564527/100‑1 (MQ=255)
            aTTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatctttt                                                                                  >  1:1445578/1‑100 (MQ=255)
             ttCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGtttatctttta                                                                                 <  2:2822490/100‑1 (MQ=255)
             ttCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatctttta                                                                                 <  1:2586590/100‑1 (MQ=255)
              tCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatcttttat                                                                                >  2:47196/1‑100 (MQ=255)
               ccTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatcttttatc                                                                               <  2:2852818/100‑1 (MQ=255)
               ccTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatcttttatc                                                                               <  1:2366564/100‑1 (MQ=255)
                cTTTAATTTTACGTCCACAGGCGTGGCTGCTTCATCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatcttttatct                                                                              >  1:1916053/1‑100 (MQ=255)
                cTTTAATTTTACGTCCACAGGCGTGGCTGCCTCCTCGCCATTACGGCTAGGTTCTAAGATCCATTCGCTGTTGGGCGCGAATCCCGtttatgctttatc                                                                               >  1:2588089/1‑99 (MQ=255)
                cTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGtttatcttttatct                                                                              >  1:2131646/1‑100 (MQ=255)
                cTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGtttatcttttatct                                                                              >  1:2122664/1‑100 (MQ=255)
                 tttAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTg                                                                             >  2:2033685/1‑100 (MQ=255)
                  ttAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATGTgg                                                                            >  1:2409891/1‑100 (MQ=255)
                  ttAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTgg                                                                            <  1:1940092/100‑1 (MQ=255)
                   tAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGgt                                                                           >  1:2029240/1‑100 (MQ=255)
                   tAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGgt                                                                           >  1:522735/1‑100 (MQ=255)
                    aaTTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGgtg                                                                          <  2:1170131/100‑1 (MQ=255)
                     aTTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCGCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGgtgt                                                                         <  2:2620506/100‑1 (MQ=255)
                      ttttACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTc                                                                        <  1:292566/100‑1 (MQ=255)
                          aCGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGAt                                                                     <  1:1964828/99‑1 (MQ=255)
                           cGTCCACAGGCGTGGCTGCATGGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAGTCCCGTTTATCTTTTATCTGGTGTCGAtg                                                                    >  1:1436746/1‑99 (MQ=255)
                           cGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGAtgt                                                                   >  1:2726261/1‑100 (MQ=255)
                              ccACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGAtgtttg                                                                <  1:2442362/100‑1 (MQ=255)
                              ccACAGGCGTGACTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGGtgtttg                                                                >  1:25179/1‑100 (MQ=255)
                               cacaGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGAtgtttt                                                                <  1:1295765/99‑2 (MQ=255)
                                acaGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGAtgtttgtt                                                              <  2:1209281/100‑1 (MQ=255)
                                   ggCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTtggc                                                           >  2:786838/1‑100 (MQ=255)
                                     cGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTtggctg                                                         <  2:1557349/100‑1 (MQ=255)
                                     cGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTtggctg                                                         <  2:2804872/100‑1 (MQ=255)
                                      gTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTtggctgg                                                        <  1:180712/100‑1 (MQ=255)
                                      gTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTtggctgg                                                        >  1:1707400/1‑100 (MQ=255)
                                       tGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCAATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTtggctggc                                                       >  2:1640478/1‑100 (MQ=255)
                                        ggCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTggctggct                                                      >  1:150062/1‑100 (MQ=255)
                                          cTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGctggctgg                                                    <  1:2819502/100‑1 (MQ=255)
                                          cTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGctggctgg                                                    <  1:2819573/100‑1 (MQ=255)
                                           tGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGa                                                   >  2:927748/1‑100 (MQ=255)
                                            gCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAg                                                  >  2:2729580/1‑100 (MQ=255)
                                              aTCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAgcg                                                <  2:1406692/100‑1 (MQ=255)
                                              aTCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAgcg                                                >  1:1077442/1‑100 (MQ=255)
                                                cgtcgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCa                                              >  2:2589798/1‑100 (MQ=255)
                                                cgtcgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCa                                              <  2:2577238/100‑1 (MQ=255)
                                                  tcgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGTGCGAATCCCGTTTATCTTTCATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCaaa                                            >  1:1583200/1‑100 (MQ=255)
                                                   cgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAAc                                           >  1:1005030/1‑100 (MQ=255)
                                                   cgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAAc                                           >  2:345255/1‑100 (MQ=255)
                                                   cgCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAAc                                           <  1:2337794/100‑1 (MQ=255)
                                                    nccATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACg                                          >  1:2558704/2‑100 (MQ=255)
                                                     ccATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGc                                         <  1:2589787/100‑1 (MQ=255)
                                                      cATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGATTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCt                                        >  2:2492871/1‑100 (MQ=255)
                                                        ttACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCa                                      <  1:927746/100‑1 (MQ=255)
                                                        ttACGGCGAGGTGCTAAGCCCCATTCACTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCa                                      <  1:2870891/100‑1 (MQ=255)
                                                         tACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCAc                                     <  1:1101011/100‑1 (MQ=255)
                                                          aCGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCAcc                                    <  2:2348427/100‑1 (MQ=255)
                                                            ggCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGTTGGCTGGAGCGCAAACGCTCACCGc                                  <  1:2201846/100‑1 (MQ=255)
                                                            ggCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGc                                  <  2:2181209/100‑1 (MQ=255)
                                                              cGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTc                                <  1:1647560/100‑1 (MQ=255)
                                                                   tGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATcc                           <  2:2438989/100‑1 (MQ=255)
                                                                   tGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATcc                           <  2:671671/100‑1 (MQ=255)
                                                                   tGCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATcc                           <  1:1008351/100‑1 (MQ=255)
                                                                    gCTAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCg                          <  2:1443589/100‑1 (MQ=255)
                                                                      tAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgt                        <  1:2238721/100‑1 (MQ=255)
                                                                      tAAGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgt                        <  1:1293816/100‑1 (MQ=255)
                                                                      tAAGCCCCATGCGCTGGGGGGGGCGAATCGGGGTTACCGCTTATCTCGTGTCGATGGTTGTTGGCTGTCTGTAGCACAAACGCTCACCGCTCGATCCGgt                        <  1:1216135/100‑1 (MQ=255)
                                                                       aaGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgt                        <  2:2530230/99‑1 (MQ=255)
                                                                        aGCTCCATTCGCTGGTGGGGGCGGATTCCCTTTATCGTTGATCTGGTGTCGAGGTTTGTTAGCTGGCTGCGGCGCATACGCTCACCGCTCGCTCCGgtan                      <  1:160357/100‑2 (MQ=255)
                                                                        aGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtag                      <  2:2205629/100‑1 (MQ=255)
                                                                        aGCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtag                      <  2:2889320/100‑1 (MQ=255)
                                                                         gCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGAGGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtagt                     >  1:859305/1‑100 (MQ=255)
                                                                         gCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtagt                     >  1:2371987/1‑100 (MQ=255)
                                                                         gCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtagt                     >  1:1101751/1‑100 (MQ=255)
                                                                         gCCCCATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtagt                     >  2:2795837/1‑100 (MQ=255)
                                                                          ccccATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGgtagta                    >  1:1378944/1‑100 (MQ=255)
                                                                          ccccATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTCTCGATGTTTGTTGGCTGGCTGGAGCGCCAACGCTCACCGCTCGATCCGgtagt                     >  1:707259/1‑99 (MQ=255)
                                                                           cccATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTAc                   <  1:634604/100‑1 (MQ=255)
                                                                           cccATTCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTAc                   >  2:1656914/1‑100 (MQ=255)
                                                                               ttCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATc               <  2:917233/100‑1 (MQ=255)
                                                                               ttCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATc               >  2:2530768/1‑100 (MQ=255)
                                                                                tCGCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCg              <  2:689949/100‑1 (MQ=255)
                                                                                  gCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCt            <  2:788959/100‑1 (MQ=255)
                                                                                  gCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCt            <  2:1071414/100‑1 (MQ=255)
                                                                                  gCTGGTGGGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCt            <  2:1555410/100‑1 (MQ=255)
                                                                                     ggtggGGGCGAATACCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGt         >  2:1977330/1‑100 (MQ=255)
                                                                                      gtggGGGCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTc        <  2:2269326/100‑1 (MQ=255)
                                                                                        gggggCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAg      <  1:2811069/100‑1 (MQ=255)
                                                                                          gggCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCACACGCTCACCGCCCGATCCGGTAGTACGACCGCTTGt         <  1:540272/95‑1 (MQ=255)
                                                                                           ggCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAgcgc   >  2:1260525/1‑100 (MQ=255)
                                                                                           ggCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAgcg    <  1:2549299/99‑1 (MQ=255)
                                                                                            gCGAATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAgcgcg  <  1:533292/100‑1 (MQ=255)
                                                                                               |                                                                                                
CCGCTTAGCAAAATTCCTTTAATTTTACGTCCACAGGCGTGGCTGCATCGTCGCCATTACGGCGAGGTGCTAAGCCCCATTCGCTGGTGGGGGCGGATCCCGTTTATCTTTTATCTGGTGTCGATGTTTGTTGGCTGGCTGGAGCGCAAACGCTCACCGCTCGATCCGGTAGTACGATCGCTTGTCAGCGCG  >  NC_000913/1835527‑1835718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑