BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,333	T→G	16.0%	E199A (GAG→GCG) ‡	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,333	0	T	G	16.0%	271.5 / 47.8	112	E199A (GAG→GCG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base T (53/41); new base G (9/9); total (62/50)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

Only 100 of 112 total aligned reads displayed.

GTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAT  >  NC_000913/1856236‑1856430

      aCCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaaa                                                                                           >  2:2518340/1‑100 (MQ=255)

       cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                          >  2:1805357/1‑100 (MQ=255)

       cccGTTCTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAt                                                                                          >  1:217187/1‑100 (MQ=255)

        ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                         <  2:42319/100‑1 (MQ=255)

        ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                         <  2:69406/100‑1 (MQ=255)

        ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAtt                                                                                         <  2:1268420/100‑1 (MQ=255)

         cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCATCAAGTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGATGCAAAAtt                                                                                         <  1:1276684/99‑1 (MQ=255)

         cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTa                                                                                        <  2:1582926/100‑1 (MQ=255)

          gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                       <  2:2126128/100‑1 (MQ=255)

          gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                       <  1:2590264/100‑2 (MQ=255)

          gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                       <  1:2094346/100‑2 (MQ=255)

          gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                       <  2:1817666/100‑2 (MQ=255)

           ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                      <  1:738798/100‑1 (MQ=255)

           ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact                                                                                      <  2:1599959/100‑3 (MQ=255)

           ttttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact                                                                                      <  2:2375797/100‑3 (MQ=255)

            tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                     <  1:2581034/100‑4 (MQ=255)

             ttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                      >  2:764399/1‑98 (MQ=255)

              tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGaaa                                                                                   <  1:2782521/100‑1 (MQ=255)

               aaTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                  >  2:786724/1‑100 (MQ=255)

                aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                 >  2:200356/1‑100 (MQ=255)

                aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                 >  2:199021/1‑100 (MQ=255)

                  caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATTTAGTGTCTCTTTCGCGGTGAGTAATTTTGCTTCGGCAAAATTAGGAAACAg                                                                                >  1:2400497/1‑99 (MQ=255)

                  caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGa                                                                               <  2:2384357/100‑1 (MQ=255)

                  caccacCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCACGGTGAGTAATTTTGCCTCGGCAAAATTCGGAAAAAGa                                                                               >  2:2427050/1‑100 (MQ=255)

                   accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGAt                                                                              >  2:685454/1‑100 (MQ=255)

                    ccaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                             >  1:283995/1‑100 (MQ=255)

                     caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATaa                                                                            >  1:2410282/1‑100 (MQ=255)

                     caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCCCTTTCCCGGAGAGTAATTTTGCCTACGCAAAATTAGGAAACAGATaa                                                                            >  1:1962052/1‑100 (MQ=255)

                          tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                       >  1:1962051/1‑100 (MQ=255)

                          tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                       <  1:990099/100‑1 (MQ=255)

                           ttttACGCCGCACGAAAGAAAGCAGTCCGCAATTTCATACGGTGTCTCTTTCCCGGTGCGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACa                                                                      <  1:2427039/100‑1 (MQ=255)

                             ttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATa                                                                    >  2:1258821/1‑100 (MQ=255)

                               aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCCAAATTAGGAAACAGATAATCGACATAAc                                                                  >  1:1866186/1‑100 (MQ=255)

                                cgccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACt                                                                 >  2:170721/1‑100 (MQ=255)

                                 gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACt                                                                 <  2:2900928/99‑1 (MQ=255)

                                 gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                >  1:1019271/1‑100 (MQ=255)

                                 gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTGCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                <  1:2757084/100‑1 (MQ=255)

                                  ccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCa                                                               >  1:1471984/1‑100 (MQ=255)

                                  ccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCa                                                               >  1:750914/1‑100 (MQ=255)

                                    gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                             <  1:1423102/100‑1 (MQ=255)

                                    gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAt                                                             >  1:2401829/1‑100 (MQ=255)

                                      acgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGc                                                           <  2:563607/100‑1 (MQ=255)

                                         caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTc                                                        <  1:1051040/100‑1 (MQ=255)

                                          aaGAAAGCAGTCAGCAATTTCCTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGc                                                           <  1:661494/96‑1 (MQ=255)

                                           agagagCAGTTAGCAATTGCAGCCAGTGGCTCTTGCCCGGTGAGTAATGTTGCCTCGGCAAAATTAGGAAACAGAGAAGCGACATAACTCAATGCTTCGc                                                      <  1:1179271/100‑1 (MQ=255)

                                           aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                      <  1:2861456/100‑1 (MQ=255)

                                           aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                      <  1:2861316/100‑1 (MQ=255)

                                            gtaaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCa                                                     <  1:2476857/98‑1 (MQ=255)

                                                  aGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatc                                               >  2:1796765/1‑100 (MQ=255)

                                                   gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatca                                              >  1:130970/1‑100 (MQ=255)

                                                     cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                            >  2:651465/1‑100 (MQ=255)

                                                     cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                            <  2:430462/100‑1 (MQ=255)

                                                     cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                            >  1:2071107/1‑100 (MQ=255)

                                                     cAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                            <  2:2708770/100‑1 (MQ=255)

                                                      aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATcc                                           >  1:1666451/1‑100 (MQ=255)

                                                      aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATcc                                           <  1:438015/100‑1 (MQ=255)

                                                         aaTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGc                                        >  2:867916/1‑100 (MQ=255)

                                                         aaTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGc                                        <  1:1141014/100‑1 (MQ=255)

                                                          aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCg                                       >  2:1327700/1‑100 (MQ=255)

                                                          aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCg                                       >  2:1767197/1‑100 (MQ=255)

                                                              cATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttc                                   <  1:220966/100‑1 (MQ=255)

                                                               aTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttca                                  >  1:2142740/1‑100 (MQ=255)

                                                               aTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttc                                   >  2:209455/1‑99 (MQ=255)

                                                                tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                 >  2:1358092/1‑100 (MQ=255)

                                                                tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                 <  1:1928574/100‑1 (MQ=255)

                                                                tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                 <  2:2626146/100‑1 (MQ=255)

                                                                 ccAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt                                <  2:2036769/100‑1 (MQ=255)

                                                                   aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattca                              >  1:252840/1‑100 (MQ=255)

                                                                       tctcTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcg                          >  2:2038545/1‑100 (MQ=255)

                                                                       tctcTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcg                          >  2:2359225/1‑100 (MQ=255)

                                                                        ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATACCTCAATACTTCGCCAATATCATCCAGCGTTTCATTCAACcgc                         >  1:2596012/1‑100 (MQ=255)

                                                                         tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                        >  1:136008/1‑100 (MQ=255)

                                                                          ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATACCTCAATGCCTCGCAAATATCATCCAGCGTTTCATTCAAcc                           >  1:527252/1‑96 (MQ=255)

                                                                          ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                       >  2:2169442/1‑100 (MQ=255)

                                                                             tCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                    >  1:905656/1‑100 (MQ=255)

                                                                               ccGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGtttta                  <  2:1973715/100‑3 (MQ=255)

                                                                                cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                 <  1:1736737/100‑1 (MQ=255)

                                                                                cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                 <  2:271193/100‑1 (MQ=255)

                                                                                 ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                >  2:1916705/1‑100 (MQ=255)

                                                                                 ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                <  1:355073/100‑1 (MQ=255)

                                                                                  tttcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa               >  2:2851316/6‑100 (MQ=39)

                                                                                    tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata             >  2:2772144/4‑100 (MQ=255)

                                                                                    tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata             >  1:688149/4‑100 (MQ=255)

                                                                                    gAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata             >  2:1502934/1‑100 (MQ=255)

                                                                                     cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat            >  2:1031295/3‑100 (MQ=255)

                                                                                     cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat            >  2:746470/3‑100 (MQ=255)

                                                                                     aGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat            >  2:1779472/1‑100 (MQ=255)

                                                                                      ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc           >  2:388535/2‑100 (MQ=255)

                                                                                      ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc           >  2:2334282/2‑100 (MQ=255)

                                                                                      ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc           >  1:2008351/2‑100 (MQ=255)

                                                                                      ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCAACCAGCGTTTCATTCAACCGCGGTTTGATCATATc           >  1:1534660/2‑100 (MQ=255)

                                                                                        aaTTTTGCCTCGGCAAAATTAGGAAACAGATACTCGACATAACTCCATGCTTCGCAAATATCATCCCGCGTTTCATTCAACCGCGGTTTGATCATATCg          <  1:2031045/99‑1 (MQ=255)

                                                                                        aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg         >  2:2082370/1‑100 (MQ=255)

                                                                                        aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCgg         <  1:685452/100‑1 (MQ=255)

                                                                                         aTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGc        <  1:1575112/100‑1 (MQ=255)

                                                                                          ttttGCCTCGGCAAAATTAGGAAACAGATAATTGACATAGCTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGca       <  1:2038538/100‑1 (MQ=255)

                                                                                            ttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGcaca     <  2:1168337/100‑1 (MQ=255)

                                                                                              gCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGa   >  2:1856893/1‑100 (MQ=255)

                                                                                               ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt  >  2:1834419/1‑100 (MQ=255)

                                                                                               ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt  <  1:651464/100‑1 (MQ=255)

GTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAT  >  NC_000913/1856236‑1856430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`