BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	410,479	A→G	100%	S112S (TCA→TCG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,479	0	A	G	99.1%	381.6 / ‑4.7	107	S112S (TCA→TCG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (62/44); total (63/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 107 total aligned reads displayed.

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCG  >  NC_000913/410382‑410575

cAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGa                                                                                                <  2:1289700/100‑1 (MQ=255)

 aaCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                               >  1:1216917/1‑100 (MQ=255)

  aCCTGGCTCAACGGTCAGCCATTCGATAAAGACGTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                               <  1:1226751/99‑1 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                             <  2:1354164/100‑1 (MQ=255)

   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                             <  2:1786480/100‑1 (MQ=255)

    cTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCa                                                                                            >  2:1921653/1‑100 (MQ=255)

     tGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGTGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAg                                                                                           <  2:321323/100‑1 (MQ=255)

       gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                         <  2:2259162/100‑1 (MQ=255)

       gCTCAACGGTCAGACATTCGATAAAGACTTAAGCGCGACGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                         >  1:1798788/1‑100 (MQ=255)

            aCGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATgg                                                                                    >  1:610109/1‑100 (MQ=255)

             cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAATCAGTAGATGgc                                                                                   <  1:1520574/100‑1 (MQ=255)

             cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgc                                                                                   >  2:2822940/1‑100 (MQ=255)

             cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgc                                                                                   >  2:104273/1‑100 (MQ=255)

             cGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGgc                                                                                   >  1:1657195/1‑100 (MQ=255)

                tCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGGAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGcgcg                                                                                <  2:1637429/100‑1 (MQ=255)

                 cAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCgg                                                                               >  1:2226420/1‑100 (MQ=255)

                  aGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGc                                                                              <  2:2320034/100‑1 (MQ=255)

                  aGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGc                                                                              <  2:281349/100‑1 (MQ=255)

                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                             >  2:2572869/1‑100 (MQ=255)

                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                             >  1:1188556/1‑100 (MQ=255)

                     cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGc                                                                           >  1:171897/1‑100 (MQ=255)

                     cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGc                                                                           >  1:895915/1‑100 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                          >  1:2835630/1‑100 (MQ=255)

                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                          >  1:2160682/1‑100 (MQ=255)

                        tCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCggg                                                                        <  1:183965/100‑1 (MQ=255)

                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                       >  1:2231195/1‑100 (MQ=255)

                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGa                                                                       >  1:2697734/1‑100 (MQ=255)

                           aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgc                                                                     >  2:639942/1‑100 (MQ=255)

                            tAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcg                                                                    >  2:468924/1‑100 (MQ=255)

                             aaaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgc                                                                   <  2:1758441/100‑1 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGTGGGAGCGCa                                                                  >  1:856159/1‑100 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                  >  2:854314/1‑100 (MQ=255)

                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                  >  1:743064/1‑100 (MQ=255)

                               aGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAg                                                                 >  2:597320/1‑100 (MQ=255)

                               aGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAg                                                                 >  1:728408/1‑100 (MQ=255)

                                gACTTAAGCGCGAGGTGGCAGCGGGAAGTGCGGCTGGCAAATGAGGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcgcn                                                                  <  1:2903208/98‑2 (MQ=255)

                                  cTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                              <  2:2231205/100‑1 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACgg                                                             <  1:1532696/99‑1 (MQ=255)

                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACgg                                                             <  2:2534199/99‑1 (MQ=255)

                                     aaGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTa                                                           >  2:1891847/1‑100 (MQ=255)

                                      aGCGCGAGGTTTCTGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGATGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACg                                                              <  1:1196936/96‑1 (MQ=255)

                                      aGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                          >  2:2473508/1‑100 (MQ=255)

                                      aGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                          >  1:1032710/1‑100 (MQ=255)

                                      aGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                          >  1:1520982/1‑100 (MQ=255)

                                       gcgcGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAtt                                                         >  1:2084635/1‑100 (MQ=255)

                                         gcgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTGTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTg                                                       >  1:511100/1‑100 (MQ=255)

                                            ccgatctaGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCg                                                    <  2:579348/93‑1 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                   <  2:2629333/100‑1 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                   >  2:1446327/1‑100 (MQ=255)

                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                   >  1:1173811/1‑100 (MQ=255)

                                              gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGGGCGCAGACGGTATTTGCCGTg                                                  <  1:646965/100‑1 (MQ=255)

                                              gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                  <  2:1407005/100‑1 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGan                                                <  2:2266830/100‑2 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAt                                                <  2:2708416/100‑1 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAt                                                >  1:2068515/1‑100 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGGGCGCAGACGGTATTTGCCGTGAt                                                >  2:2317437/1‑100 (MQ=255)

                                                tGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGAATGGGAAGCGGTAGATGGCGGGGCAGGGGGAGCGCGGACCCTGTTTCCCGTGa                                                 >  1:1274363/1‑99 (MQ=255)

                                                  cAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTa                                              >  2:1957039/1‑100 (MQ=255)

                                                   aGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTAt                                             >  2:1480302/1‑100 (MQ=255)

                                                   aGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGAGAtcgg                                             >  1:579347/1‑97 (MQ=255)

                                                     cGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCg                                           <  2:18714/100‑1 (MQ=255)

                                                       ggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGc                                         <  1:1384588/100‑1 (MQ=255)

                                                        gAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCa                                        >  2:945504/1‑100 (MQ=255)

                                                           gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                     >  1:144478/1‑100 (MQ=255)

                                                           gTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACgg                                     >  1:144457/1‑100 (MQ=255)

                                                              cGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAt                                  >  1:304227/1‑100 (MQ=255)

                                                               ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATg                                 >  1:388880/1‑100 (MQ=255)

                                                               ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATg                                 <  2:1597209/100‑1 (MQ=255)

                                                               ggCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATg                                 >  1:2874818/1‑100 (MQ=255)

                                                                  tGGCAAATGACGCTAACTGTCTGGCTGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcgg                              <  1:2476660/100‑1 (MQ=255)

                                                                  tGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcgg                              <  2:2068522/100‑1 (MQ=255)

                                                                   ggCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcggc                             >  2:1197668/1‑100 (MQ=255)

                                                                   ggCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcggc                             >  2:1906556/1‑100 (MQ=255)

                                                                   ggCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgcggc                             >  1:2036570/1‑100 (MQ=255)

                                                                     cAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgcgc                           >  1:2699552/1‑100 (MQ=255)

                                                                      aaaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGcgcg                          <  2:1993736/100‑1 (MQ=255)

                                                                      aaaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGcgcg                          >  2:1633933/1‑100 (MQ=255)

                                                                           aCGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGt                     <  2:2642685/100‑1 (MQ=255)

                                                                            cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                    <  1:2599617/100‑1 (MQ=255)

                                                                            cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                    >  2:1633588/1‑100 (MQ=255)

                                                                            cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                    <  1:602041/100‑1 (MQ=255)

                                                                            cGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTg                    <  2:1874650/100‑1 (MQ=255)

                                                                             gCTAACTGTTAGGCGGTTTCGGAAGCAGGAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCAGTCATTATCGGCACGGGATGCGGCGCGGGCGTg                    <  1:1916085/99‑1 (MQ=255)

                                                                             gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                   >  2:1988577/1‑100 (MQ=255)

                                                                              cgaaCTGTCTGGCGGTGTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                  <  2:1336601/98‑1 (MQ=255)

                                                                               tagcTGTCTGGTGGTTTCGGAAGCAGGAGATGGGGCGGCAGCGGGGGCGCAGACGGTATTTGCCGCGATTATCGGCGCGGGATGCGGCGCGGGCGTGGCa                 <  1:663413/97‑1 (MQ=255)

                                                                               tAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCa                 >  2:2399520/1‑100 (MQ=255)

                                                                                aaCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAt                >  2:1922704/1‑100 (MQ=255)

                                                                                 aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCAGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAtt               >  1:1400367/1‑100 (MQ=255)

                                                                                   tGTCTGGGGGTTTCGGAAGCATTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCTGCACGGGATGCGGCGCGGGCGTGGc                  <  1:2312397/95‑1 (MQ=255)

                                                                                   tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa             <  1:396760/100‑1 (MQ=255)

                                                                                   tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa             <  1:397085/100‑1 (MQ=255)

                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAAt           <  1:962377/99‑1 (MQ=255)

                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg          <  2:1479962/100‑1 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        >  1:1980726/1‑100 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        >  1:465778/1‑100 (MQ=255)

                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg        >  1:135839/1‑100 (MQ=255)

                                                                                           ggTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggacgg     >  2:2096572/1‑100 (MQ=255)

                                                                                            gTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcggg    >  2:711674/1‑100 (MQ=255)

                                                                                              ttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGggcgggcg  >  2:2638203/1‑100 (MQ=255)

CAACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCG  >  NC_000913/410382‑410575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`