Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 17.0% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT17.0% 234.3 / 37.6 94I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base A (35/43);  new base T (10/6);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATG  >  NC_000913/2081589‑2081786
                                                                                                   |                                                                                                  
ggTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                    >  2:2532344/1‑100 (MQ=255)
 gTAGCACCATCCAGAACTCGTGTGCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAt                                                                                                   <  1:2529600/100‑1 (MQ=255)
  tAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAttt                                                                                                  <  2:2134631/100‑1 (MQ=255)
    gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTg                                                                                                <  2:614259/100‑1 (MQ=255)
     cACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGc                                                                                               <  1:2632248/100‑1 (MQ=255)
     cACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGc                                                                                               <  1:936122/100‑1 (MQ=255)
      aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa                                                                                              >  1:1499571/1‑100 (MQ=255)
       ccATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                             <  1:1303541/100‑1 (MQ=255)
       ccATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                             >  2:1416395/1‑100 (MQ=255)
        cATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATCTTCTTTATCGCCATACCAGATGCTTTCCGCTCCGACCTTTTTCCCACGGTGCACAATTTGCAc                                                                                             >  2:704655/1‑99 (MQ=255)
          tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgt                                                                                          >  1:1383136/1‑100 (MQ=255)
           ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgt                                                                                          <  1:2207332/99‑1 (MQ=255)
            cAGATCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                        <  1:2055228/100‑3 (MQ=255)
            cAGAACTCTTGTTCCGTTCCCAGGTCGTTATTTTCTTTATCGCCATACCAGATGCGTTCCGTGCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                        <  1:392586/100‑3 (MQ=255)
            cAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                        <  2:2494597/100‑3 (MQ=255)
            cAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgtag                                                                                        <  1:496674/100‑1 (MQ=255)
              gAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                        <  1:297330/98‑3 (MQ=255)
               aaCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgggaa                                                                                     <  2:389313/100‑6 (MQ=255)
                     tGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGtttt                                                                               >  2:322316/1‑100 (MQ=255)
                      gttgcgtttcCAGGTCGATATTTTCTTTATCGCCATACTAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                              <  1:512405/100‑1 (MQ=255)
                      gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                              >  2:1158132/1‑100 (MQ=255)
                      gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                              >  2:878354/1‑100 (MQ=255)
                       ttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTCTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                             <  1:1790386/100‑1 (MQ=255)
                        tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                            <  2:2294246/100‑1 (MQ=255)
                        tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                            <  2:603109/100‑1 (MQ=255)
                        tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgtt                                                                            >  2:1180687/1‑100 (MQ=255)
                         ccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgttg                                                                           >  2:182707/1‑100 (MQ=255)
                         ccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgttg                                                                           <  1:322314/100‑1 (MQ=255)
                          cgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGc                                                                          >  2:2448787/1‑100 (MQ=255)
                            ttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccc                                                                        <  1:1482021/100‑1 (MQ=255)
                             tccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGATGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcccc                                                                       <  1:1052060/100‑1 (MQ=255)
                              cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccccc                                                                      >  2:211315/1‑100 (MQ=255)
                              cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccccc                                                                      >  1:412964/1‑100 (MQ=255)
                              cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCAACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGccccc                                                                      <  1:1440798/100‑1 (MQ=255)
                               ccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCa                                                                     <  2:2036468/100‑1 (MQ=255)
                                cAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCaa                                                                    >  2:2517863/1‑100 (MQ=255)
                                  ggTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACg                                                                  <  1:1297089/100‑1 (MQ=255)
                                  ggTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGc                                                                 <  2:334618/100‑1 (MQ=255)
                                   gTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACgn                                                                 <  2:2478128/100‑2 (MQ=255)
                                     cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACTGTGCAAAATTTGTACCGTAGTAAAGTTTTGTTGccccc                                                                      >  1:2290323/1‑93 (MQ=255)
                                         atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACTGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAt                                                           >  2:1321703/1‑100 (MQ=255)
                                         atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAt                                                           <  2:2431977/100‑1 (MQ=255)
                                          ttttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAtt                                                          <  1:874446/100‑1 (MQ=255)
                                           tttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAttt                                                         >  2:750381/1‑100 (MQ=255)
                                              cTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                                                      <  2:2058625/100‑1 (MQ=255)
                                              cTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                                                      >  1:1463943/1‑100 (MQ=255)
                                                ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                    <  2:2444455/100‑1 (MQ=255)
                                                 tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                                                   >  1:213337/1‑100 (MQ=255)
                                                  aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTGAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                  >  2:1590338/1‑100 (MQ=255)
                                                  aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                                                  <  2:412966/100‑1 (MQ=255)
                                                    tccgatctcAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                                                <  2:1806205/92‑1 (MQ=255)
                                                    agCCATACCAGATACGCTAAGTTCCGACATTTATCCCACGGCGCAGAATTTGCACCGTAGGAAAGTCTTGTTGCCCCCAACGCATTAATTGTCTTTCct                                                 >  1:2424986/2‑99 (MQ=255)
                                                      ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCtt                                              >  1:2122584/1‑100 (MQ=255)
                                                        aTACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTcc                                            <  2:1074271/100‑1 (MQ=255)
                                                        aTACCAGATGCGTTCCGTTCCGACCTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCt                                           >  2:696002/1‑100 (MQ=255)
                                                           ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                         <  2:2200991/100‑1 (MQ=255)
                                                           ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                         <  2:737474/100‑1 (MQ=255)
                                                            cAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctcagatcgga                                        >  1:1806199/1‑92 (MQ=255)
                                                            cAGATGCGTTCCGTTCCGAACTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCAc                                        >  1:1244199/1‑100 (MQ=255)
                                                                 gCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt                                   <  2:1351714/100‑1 (MQ=255)
                                                                      ccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc                              >  2:1665780/1‑100 (MQ=255)
                                                                      ccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc                              <  2:1296481/100‑1 (MQ=255)
                                                                       cgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                             >  2:2740504/1‑100 (MQ=255)
                                                                       cgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                             >  2:155919/1‑100 (MQ=255)
                                                                        gttccgACTTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAc                            <  2:2015021/100‑1 (MQ=255)
                                                                        gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAc                            <  2:2841209/100‑1 (MQ=255)
                                                                        gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAc                            <  2:549782/100‑1 (MQ=255)
                                                                        gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAc                            <  2:2488250/100‑1 (MQ=255)
                                                                            cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                        <  2:1574728/100‑1 (MQ=255)
                                                                            cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                        >  1:6873/1‑100 (MQ=255)
                                                                              aCCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAg                      >  2:2706596/1‑100 (MQ=255)
                                                                                cTTTTTCCCACGGTGCAAAATTTGCACCGTAGTGAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                    >  1:1741265/1‑100 (MQ=255)
                                                                                  ttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc                  <  1:1839356/100‑1 (MQ=255)
                                                                                  ttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc                  <  2:1021095/100‑1 (MQ=255)
                                                                                  ttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc                  <  2:2697959/100‑1 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACTGTCGTAAAGTTTTGTTGACCCCAACACAGTAATCTGCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa             >  1:2595056/3‑100 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa             >  1:1119082/3‑100 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa             >  1:6539/3‑100 (MQ=255)
                                                                                       ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCAAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa             >  1:719391/3‑100 (MQ=255)
                                                                                        cACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAc            <  2:920816/100‑1 (MQ=255)
                                                                                        cACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAc            <  2:13162/100‑1 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc           >  2:2248405/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc           >  2:2132332/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc           >  1:2750523/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAcc           >  2:1287152/1‑100 (MQ=255)
                                                                                         aCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTAAGCCCCCATCAcc           >  2:2900228/1‑100 (MQ=255)
                                                                                          cGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa          >  2:2484485/1‑100 (MQ=255)
                                                                                          cGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa          <  1:2798178/100‑1 (MQ=255)
                                                                                           ggTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCCCGACCTTTTGCACTGTCAGCCCCCCTTACCAc         >  1:362869/1‑100 (MQ=255)
                                                                                            gTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACt        <  2:1887244/100‑1 (MQ=255)
                                                                                             tGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTg       <  1:748498/100‑1 (MQ=255)
                                                                                              gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc      >  2:511609/1‑100 (MQ=255)
                                                                                              gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc      >  2:1872316/1‑100 (MQ=255)
                                                                                              gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc      >  1:1248654/1‑100 (MQ=255)
                                                                                              gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc      <  2:2582598/100‑1 (MQ=255)
                                                                                               cAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCa     >  2:811761/1‑100 (MQ=255)
                                                                                                aaaaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCaa    >  1:1823450/1‑100 (MQ=255)
                                                                                                 aaaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAAt   <  2:463627/100‑1 (MQ=255)
                                                                                                  aaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATg  <  2:1499574/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATG  >  NC_000913/2081589‑2081786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: