Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,800,515 C→A 15.6% intergenic (+40/‑208) ygaM → / → nrdH putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,5150CA15.6% 320.9 / 42.1 122intergenic (+40/‑208)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base C (60/41);  new base A (9/10);  total (70/52)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 127 total aligned reads displayed.

GGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATT  >  NC_000913/2800416‑2800614
                                                                                                   |                                                                                                   
tgTACAGCGGCAGCGGTTGGTATTTTTAGCGGCGCGCTGTTGGGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                                     >  1:1585235/2‑100 (MQ=255)
ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                                     >  2:2563810/1‑100 (MQ=255)
 gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGcc                                                                                                    <  2:521338/100‑1 (MQ=255)
  tACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCt                                                                                                   >  1:1356718/1‑100 (MQ=255)
  tACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCt                                                                                                   >  1:1426610/1‑100 (MQ=255)
   aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATTATGATAAATACGCGTCTTTGACCCCGAAGCCTg                                                                                                  <  1:1916611/100‑1 (MQ=255)
   aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTg                                                                                                  <  1:1801819/100‑1 (MQ=255)
   aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCAAAGCCTg                                                                                                  <  1:2042028/100‑1 (MQ=255)
    cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGt                                                                                                 >  1:1386549/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  1:476609/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  2:591994/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  1:205711/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  1:852012/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  1:1603500/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  1:2733690/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               >  1:476821/1‑100 (MQ=255)
      gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt                                                                                               <  1:388468/100‑1 (MQ=255)
        agcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTc                                                                                             >  1:1877040/1‑100 (MQ=255)
        agcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTc                                                                                             <  2:1802844/100‑1 (MQ=255)
        agcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAAAGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTc                                                                                             >  1:311341/1‑100 (MQ=255)
         gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCACAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCg                                                                                            >  1:322455/1‑100 (MQ=255)
            gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgg                                                                                           <  1:1166082/98‑1 (MQ=255)
            gCGGGAGGGAATTTTATCGGCGCACTGTTAAGCATGCGCAAAAAAAAAAAAAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCgggg                                                                                         <  1:2138120/100‑1 (MQ=255)
             cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt                                                                                        <  2:676857/100‑1 (MQ=255)
             cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt                                                                                        <  2:1537645/100‑1 (MQ=255)
             cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt                                                                                        <  1:1467312/100‑1 (MQ=255)
             cGGTAGGTATTTTTATCGGCGCACGGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt                                                                                        <  1:2441613/100‑1 (MQ=255)
              ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTc                                                                                             >  1:723196/1‑94 (MQ=255)
              ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGtt                                                                                       >  2:2255167/1‑100 (MQ=255)
              ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtc                                                                                       <  2:1403823/100‑2 (MQ=255)
               gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGttt                                                                                      >  2:900495/1‑100 (MQ=255)
                taggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTc                                                                                     >  1:2177257/1‑100 (MQ=255)
                 aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCt                                                                                    <  1:349682/100‑1 (MQ=255)
                 aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtcaaa                                                                                    <  2:670101/100‑5 (MQ=38)
                  ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt                                                                                   >  1:830838/1‑100 (MQ=255)
                  ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtt                                                                                   >  2:2199528/1‑100 (MQ=255)
                  ggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtcaaag                                                                                   <  2:1134794/100‑6 (MQ=38)
                    taTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtttt                                                                                 <  2:659878/100‑1 (MQ=255)
                    taTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtttt                                                                                 >  1:1329730/1‑100 (MQ=255)
                      tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg                                                                               <  2:2739953/100‑1 (MQ=255)
                       ttttATCGGCGCACTGTTAAGCATGCGCAAATCGTATTGCAAAAATGATAATAAATACGCGACTTTGACCCCGATGCCTGTCTTCGGAGTATCttttt                                                                                >  1:1118102/1‑98 (MQ=255)
                         ttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCt                                                                            >  1:2752236/1‑100 (MQ=255)
                           aTCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTgg                                                                          >  1:27566/1‑100 (MQ=255)
                            tCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGt                                                                         <  2:2501997/100‑1 (MQ=255)
                             cGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTg                                                                        <  1:2902220/100‑1 (MQ=255)
                                cgcACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAAt                                                                     >  2:2093224/1‑100 (MQ=255)
                                   aCTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                                  >  2:1592147/1‑100 (MQ=255)
                                   aCTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                                  <  1:2093217/100‑1 (MQ=255)
                                      gTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                               <  1:472534/100‑1 (MQ=255)
                                        tAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaaa                                                             <  2:846539/100‑1 (MQ=255)
                                          aGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATc                                                           >  2:534608/1‑100 (MQ=255)
                                            cATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                                         >  1:2251004/1‑100 (MQ=255)
                                              tGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccccc                                                       <  2:296467/100‑1 (MQ=255)
                                              tGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccccc                                                       >  2:238747/1‑100 (MQ=255)
                                                cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccccc                                                       <  2:2813823/98‑1 (MQ=255)
                                                cgcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTa                                                     >  1:1260236/1‑100 (MQ=255)
                                                 gcAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAc                                                    >  1:2435063/1‑100 (MQ=255)
                                                           gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCCCGc                                          >  1:2331076/1‑100 (MQ=255)
                                                           gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGc                                          >  2:2743831/1‑100 (MQ=255)
                                                           gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGc                                          <  2:823838/100‑1 (MQ=255)
                                                           gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGc                                          >  2:413641/1‑100 (MQ=255)
                                                           gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGc                                          >  2:2139049/1‑100 (MQ=255)
                                                           gTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGAGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGc                                          >  1:2501985/1‑100 (MQ=255)
                                                            tGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGGCTGTATTCAGGGTTTCTTTTTGACTGTTGGATCACAAAAATCCCCCTATTCCGTCagg                                           >  1:1912705/1‑96 (MQ=255)
                                                            tGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt                                         >  1:2867521/1‑100 (MQ=255)
                                                             gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                        >  2:418523/1‑100 (MQ=255)
                                                             gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                        >  2:953494/1‑100 (MQ=255)
                                                             gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                        >  2:1462794/1‑100 (MQ=255)
                                                              cAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                       >  2:2906324/1‑100 (MQ=255)
                                                              cAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                       <  2:673019/100‑1 (MQ=255)
                                                              cAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                       <  2:2740445/100‑1 (MQ=255)
                                                              cAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCa                                       >  1:2189154/1‑100 (MQ=255)
                                                               aaaaaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCat                                      >  2:1457507/1‑100 (MQ=255)
                                                                 aaaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCatat                                    <  2:167998/100‑1 (MQ=255)
                                                                  aaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                                   <  2:443540/100‑1 (MQ=255)
                                                                  aaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                                   <  1:2193586/100‑1 (MQ=255)
                                                                   aTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATcc                                  <  2:2003662/100‑1 (MQ=255)
                                                                    tGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCa                                 >  1:2880257/1‑100 (MQ=255)
                                                                     gATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg                                <  2:1932520/100‑1 (MQ=255)
                                                                          taaataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTaa                           >  1:1461659/1‑100 (MQ=255)
                                                                           aaataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAAt                          <  2:796708/100‑1 (MQ=255)
                                                                               aCGCGTTTTTGAACACGAAACCTGTATTCTGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCTCCTTCCCCGTCACGCTCATATCCAGGGTAATTTc                       <  1:1362001/99‑1 (MQ=255)
                                                                                cgcgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGa                     <  2:2172681/100‑1 (MQ=255)
                                                                                 gcgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc                    <  1:2033335/100‑1 (MQ=255)
                                                                                  cgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAcc                   <  2:875816/100‑1 (MQ=255)
                                                                                         aaCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt            >  2:1668180/2‑100 (MQ=255)
                                                                                         aaCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt            >  2:59551/2‑100 (MQ=255)
                                                                                          aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTg           >  1:1787163/1‑100 (MQ=255)
                                                                                           tccgatctccTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc          <  2:1069412/92‑1 (MQ=255)
                                                                                           ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc          <  1:101676/100‑1 (MQ=255)
                                                                                           ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc          <  2:291683/100‑1 (MQ=255)
                                                                                           ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc          >  2:106993/1‑100 (MQ=255)
                                                                                           ccccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc          >  1:2560822/1‑100 (MQ=255)
                                                                                           ccccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc          >  2:171963/1‑100 (MQ=255)
                                                                                             ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCta        <  1:2414238/100‑1 (MQ=255)
                                                                                             ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCta        >  2:793457/1‑100 (MQ=255)
                                                                                             ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCta        >  1:445125/1‑100 (MQ=255)
                                                                                             ccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAAACCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTg           <  1:1993452/97‑1 (MQ=255)
                                                                                                aaGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCtatat     <  2:1724808/100‑1 (MQ=255)
                                                                                                   ccTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGcagatcgga  >  1:1069410/1‑92 (MQ=255)
                                                                                                   |                                                                                                   
GGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATT  >  NC_000913/2800416‑2800614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: