Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,039 A→T 15.5% Q231L (CAG→CTG)  queF → 7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,0390AT15.5% 305.8 / 44.6 116Q231L (CAG→CTG) queF7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)
Reads supporting (aligned to +/- strand):  ref base A (51/47);  new base T (5/13);  total (56/60)
Fisher's exact test for biased strand distribution p-value = 7.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 118 total aligned reads displayed.

GGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAA  >  NC_000913/2925940‑2926138
                                                                                                   |                                                                                                   
ggggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACa                                                                                                     <  1:167347/100‑1 (MQ=255)
 gggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAg                                                                                                    >  1:2704191/1‑100 (MQ=255)
   gTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTg                                                                                                  <  2:93631/100‑1 (MQ=255)
      cGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGt                                                                                               >  2:2647865/1‑100 (MQ=255)
       gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                              <  1:288642/100‑1 (MQ=255)
       gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                              >  2:2209922/1‑100 (MQ=255)
        cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                             >  2:2013740/1‑100 (MQ=255)
        cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                             <  2:1161217/100‑1 (MQ=255)
         tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                             >  1:1177221/1‑99 (MQ=255)
           cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAc                                                                                          <  2:334508/100‑1 (MQ=255)
           cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAAc                                                                                          <  1:1607602/100‑1 (MQ=255)
            aaaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAct                                                                                         <  2:2873128/100‑2 (MQ=255)
             aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                        <  2:1532915/100‑3 (MQ=255)
             aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                        <  1:668843/100‑3 (MQ=255)
             aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                        <  2:335174/100‑3 (MQ=255)
             aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                        <  1:2021096/100‑3 (MQ=255)
              aTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcg                                                                                       <  2:928653/100‑4 (MQ=39)
              aTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcg                                                                                       <  2:514090/100‑4 (MQ=38)
               ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                      <  1:209208/100‑5 (MQ=38)
               ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                      <  1:1869714/100‑5 (MQ=38)
                  aGTATTGTGGACGCCAAATTGAGAGGGAAGAACTGCTGCGTTACCTGGGCTCATTCCGTCATCATAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                   <  1:6447/100‑1 (MQ=255)
                  aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgttgt                                                                                   <  1:2553413/100‑8 (MQ=38)
                  aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                   <  2:545832/100‑1 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                  <  2:2623282/100‑1 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                  <  2:2286111/100‑1 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                  <  2:2708661/100‑1 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGCGGAACGCATCttt                                                                                  >  2:259091/1‑100 (MQ=255)
                   gTATCGGGGACGCCAAATTGACAGAGAAAGACTGCTGCGTTACCTGGTGTCAGTCCGTCATCACAACGAGTTCCACGGACTGTTCGTGGAActcgttgt                                                                                   <  1:2090173/99‑8 (MQ=25)
                    tATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTa                                                                                 >  2:1748544/1‑100 (MQ=255)
                       cGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCTTCATCACAACGAGTTCCACGAACAGTGCGTGGACCGCATCTTTAATg                                                                              <  2:1324839/100‑1 (MQ=255)
                        gTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGa                                                                             >  2:1234739/1‑100 (MQ=255)
                          ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                           <  2:2399382/100‑1 (MQ=255)
                          ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                           <  2:589094/100‑1 (MQ=255)
                          ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                           <  1:1917026/100‑1 (MQ=255)
                          ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                           <  2:409010/100‑1 (MQ=255)
                            aCGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATAACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTg                                                                         >  1:1044757/1‑100 (MQ=255)
                             cGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGATCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGt                                                                        >  2:2054990/1‑100 (MQ=255)
                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                      >  2:1728622/1‑100 (MQ=255)
                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                      >  2:702983/1‑100 (MQ=255)
                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                      <  1:2495216/100‑1 (MQ=255)
                                cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                     <  1:559893/100‑1 (MQ=255)
                                cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                     <  1:781529/100‑1 (MQ=255)
                                cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                     <  2:1843610/100‑1 (MQ=255)
                                cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                     <  2:352959/100‑1 (MQ=255)
                                 aaaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACg                                                                    <  1:2113662/100‑1 (MQ=255)
                                 aaaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACg                                                                    <  1:2671883/100‑1 (MQ=255)
                                      gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                               >  2:2570707/1‑100 (MQ=255)
                                      gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                               >  1:1004582/1‑100 (MQ=255)
                                         agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                            >  2:1214806/1‑100 (MQ=255)
                                         agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                            <  2:918360/100‑1 (MQ=255)
                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcn                                                            <  2:2515594/99‑2 (MQ=255)
                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                           >  2:1010501/1‑100 (MQ=255)
                                           agaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccag                                                          >  1:1079095/1‑100 (MQ=255)
                                           agaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccag                                                          >  1:1595023/1‑100 (MQ=255)
                                            gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagc                                                         <  2:1952264/100‑1 (MQ=255)
                                            gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagc                                                         <  2:1951107/100‑1 (MQ=255)
                                                    gctgcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTACCAGCCAGaaaa                                                  >  2:2522922/1‑99 (MQ=255)
                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                              <  2:1734714/100‑1 (MQ=255)
                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                              <  1:1994781/100‑1 (MQ=255)
                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                              <  2:1280821/100‑1 (MQ=255)
                                                         gTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAg                                            >  1:3069/1‑100 (MQ=255)
                                                           tACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCg                                          <  1:2100203/100‑1 (MQ=255)
                                                              cTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGttt                                       >  1:528579/1‑100 (MQ=255)
                                                                ggTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTAc                                     <  1:1010499/100‑1 (MQ=255)
                                                                 gTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACg                                    >  2:1798606/1‑100 (MQ=255)
                                                                  tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGc                                   <  2:2563883/100‑1 (MQ=255)
                                                                   ctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCa                                  >  1:2172607/1‑100 (MQ=255)
                                                                     ctttCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAGAAATTGAGCGTTTACGCACg                                >  2:434414/3‑100 (MQ=255)
                                                                     cATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGc                                   >  1:2491536/1‑97 (MQ=255)
                                                                      aTTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGt                               >  2:91532/1‑100 (MQ=255)
                                                                      aTTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGt                               >  1:2653701/1‑100 (MQ=255)
                                                                          cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtata                           <  2:2309469/100‑1 (MQ=255)
                                                                          cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtata                           >  2:582587/1‑100 (MQ=255)
                                                                           gTCATCACAACTAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAc                          >  2:792837/1‑100 (MQ=255)
                                                                           gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCATTTACGCACGTTATAc                          >  2:1781184/1‑100 (MQ=255)
                                                                              atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcg                       >  2:1355001/1‑100 (MQ=255)
                                                                              atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcg                       >  1:530307/1‑100 (MQ=255)
                                                                               tcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgt                      >  2:2823677/1‑100 (MQ=255)
                                                                                 acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCtgtcg                    >  2:1051420/1‑100 (MQ=255)
                                                                                 acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtcg                    >  2:2713534/1‑100 (MQ=255)
                                                                                 acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtcg                    >  1:2703225/1‑100 (MQ=255)
                                                                                    aCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                 >  2:925949/1‑100 (MQ=255)
                                                                                     tgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTAAGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGc                >  1:1614549/4‑100 (MQ=38)
                                                                                       cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg              >  1:2765131/2‑100 (MQ=255)
                                                                                       cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg              >  2:2778304/2‑100 (MQ=255)
                                                                                       cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg              >  1:2003636/2‑100 (MQ=255)
                                                                                       aGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg              >  2:1502589/1‑100 (MQ=255)
                                                                                        gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACTTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGt             >  1:140823/1‑100 (MQ=255)
                                                                                        gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg              >  1:2438428/1‑99 (MQ=255)
                                                                                        gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGt             >  2:2426798/1‑100 (MQ=255)
                                                                                         ttCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTc            <  2:2779746/100‑1 (MQ=255)
                                                                                          tCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCt           >  1:2485895/1‑100 (MQ=255)
                                                                                           ccACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTg          >  2:1021400/1‑100 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGATTACGCACGTTATACACGTCGTGGCGGACTgg         >  1:2167990/1‑100 (MQ=255)
                                                                                               gAACAGTGCGTGGATCGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACa      <  2:2652561/100‑1 (MQ=255)
                                                                                               gAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACa      <  1:1319467/100‑1 (MQ=255)
                                                                                                aaCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAt     <  2:1449875/100‑1 (MQ=255)
                                                                                                 aCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGGTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAtt    <  1:1021398/100‑1 (MQ=255)
                                                                                                  cAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTa   <  2:2662428/100‑1 (MQ=255)
                                                                                                   aGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTaa  >  1:2410299/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAA  >  NC_000913/2925940‑2926138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: