Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,042 G→T 15.2% C232F (TGC→TTC)  queF → 7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,0420GT15.2% 348.8 / 39.9 118C232F (TGC→TTC) queF7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)
Reads supporting (aligned to +/- strand):  ref base G (53/47);  new base T (5/13);  total (58/60)
Fisher's exact test for biased strand distribution p-value = 7.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 119 total aligned reads displayed.

GTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACC  >  NC_000913/2925943‑2926140
                                                                                                   |                                                                                                  
gTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTg                                                                                                    <  2:93631/100‑1 (MQ=255)
   cGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGt                                                                                                 >  2:2647865/1‑100 (MQ=255)
    gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                >  2:2209922/1‑100 (MQ=255)
    gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                <  1:288642/100‑1 (MQ=255)
     cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                               <  2:1161217/100‑1 (MQ=255)
     cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                               >  2:2013740/1‑100 (MQ=255)
      tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                               >  1:1177221/1‑99 (MQ=255)
       ccAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGaa                                                                                             >  2:2887080/1‑100 (MQ=255)
        cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAc                                                                                            <  2:1096495/100‑1 (MQ=255)
        cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAAc                                                                                            >  2:1987720/1‑100 (MQ=255)
        cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAAc                                                                                            <  1:1607602/100‑1 (MQ=255)
         aaaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAct                                                                                           <  2:2873128/100‑2 (MQ=255)
         aaaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACg                                                                                           <  2:2662830/100‑1 (MQ=255)
          aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                          <  1:668843/100‑3 (MQ=255)
          aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                          <  2:335174/100‑3 (MQ=255)
          aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                          <  1:2021096/100‑3 (MQ=255)
           aTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcg                                                                                         <  2:928653/100‑4 (MQ=39)
            ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                        <  1:209208/100‑5 (MQ=38)
            ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                        <  1:1869714/100‑5 (MQ=38)
               aGTATTGTGGACGCCAAATTGAGAGGGAAGAACTGCTGCGTTACCTGGGCTCATTCCGTCATCATAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                     <  1:6447/100‑1 (MQ=255)
               aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgttgt                                                                                     <  1:2553413/100‑8 (MQ=38)
               aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                     <  2:545832/100‑1 (MQ=255)
                gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                    <  2:2286111/100‑1 (MQ=255)
                gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                    <  2:2623282/100‑1 (MQ=255)
                gTATCGGGGACGCCAAATTGACAGAGAAAGACTGCTGCGTTACCTGGTGTCAGTCCGTCATCACAACGAGTTCCACGGACTGTTCGTGGAActcgttgt                                                                                     <  1:2090173/99‑8 (MQ=25)
                 tATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTa                                                                                   >  2:1748544/1‑100 (MQ=255)
                    cGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCTTCATCACAACGAGTTCCACGAACAGTGCGTGGACCGCATCTTTAATg                                                                                <  2:1324839/100‑1 (MQ=255)
                    cGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATg                                                                                <  2:1328950/100‑1 (MQ=255)
                    cGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATg                                                                                <  1:627118/100‑1 (MQ=255)
                     gTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGa                                                                               >  2:1234739/1‑100 (MQ=255)
                       ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                             <  1:1917026/100‑1 (MQ=255)
                       ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                             <  2:2399382/100‑1 (MQ=255)
                       ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                             <  2:409010/100‑1 (MQ=255)
                       ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                             <  2:589094/100‑1 (MQ=255)
                         aCGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATAACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTg                                                                           >  1:1044757/1‑100 (MQ=255)
                            ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                        >  2:702983/1‑100 (MQ=255)
                            ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                        <  1:2495216/100‑1 (MQ=255)
                            ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                        >  2:1728622/1‑100 (MQ=255)
                             cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                       <  1:781529/100‑1 (MQ=255)
                             cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                       <  2:352959/100‑1 (MQ=255)
                             cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                       <  2:1843610/100‑1 (MQ=255)
                              aaaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACg                                                                      <  1:2671883/100‑1 (MQ=255)
                              aaaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACg                                                                      <  1:2113662/100‑1 (MQ=255)
                                aTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                    <  2:1741563/100‑1 (MQ=255)
                                   gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                                 >  1:1004582/1‑100 (MQ=255)
                                   gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                                 >  2:2570707/1‑100 (MQ=255)
                                      agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                              >  2:1214806/1‑100 (MQ=255)
                                      agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                              <  2:918360/100‑1 (MQ=255)
                                       gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcn                                                              <  2:2515594/99‑2 (MQ=255)
                                       gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                             >  2:1010501/1‑100 (MQ=255)
                                        agaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccag                                                            >  1:1595023/1‑100 (MQ=255)
                                        agaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccag                                                            >  1:1079095/1‑100 (MQ=255)
                                         gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagc                                                           <  2:1952264/100‑1 (MQ=255)
                                         gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagc                                                           <  2:1951107/100‑1 (MQ=255)
                                                 gctgcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTACCAGCCAGaaaa                                                    >  2:2522922/1‑99 (MQ=255)
                                                    gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                                <  1:1994781/100‑1 (MQ=255)
                                                    gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                                <  2:1734714/100‑1 (MQ=255)
                                                      gTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAg                                              >  1:3069/1‑100 (MQ=255)
                                                        tACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCg                                            <  1:2100203/100‑1 (MQ=255)
                                                           cTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGttt                                         >  1:528579/1‑100 (MQ=255)
                                                             ggTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTAc                                       <  1:1010499/100‑1 (MQ=255)
                                                               tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGc                                     <  2:2563883/100‑1 (MQ=255)
                                                                ctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCa                                    >  1:2172607/1‑100 (MQ=255)
                                                                  ctttCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAGAAATTGAGCGTTTACGCACg                                  >  2:434414/3‑100 (MQ=255)
                                                                  cATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGc                                     >  1:2491536/1‑97 (MQ=255)
                                                                   aTTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGt                                 >  2:91532/1‑100 (MQ=255)
                                                                   aTTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGt                                 >  1:2653701/1‑100 (MQ=255)
                                                                       cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtata                             <  2:2309469/100‑1 (MQ=255)
                                                                       cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtata                             >  2:582587/1‑100 (MQ=255)
                                                                        gTCATCACAACTAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAc                            >  2:792837/1‑100 (MQ=255)
                                                                        gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCATTTACGCACGTTATAc                            >  2:1781184/1‑100 (MQ=255)
                                                                           atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcg                         >  1:530307/1‑100 (MQ=255)
                                                                           atcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcg                         >  2:1355001/1‑100 (MQ=255)
                                                                            tcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgt                        >  2:2823677/1‑100 (MQ=255)
                                                                              acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCtgtcg                      >  2:1051420/1‑100 (MQ=255)
                                                                              acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtcg                      >  1:2703225/1‑100 (MQ=255)
                                                                              acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtcg                      >  2:2713534/1‑100 (MQ=255)
                                                                                gatgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTg                    >  2:441667/6‑100 (MQ=38)
                                                                                 aCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                   >  2:2375727/1‑100 (MQ=255)
                                                                                 aCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                   >  2:925949/1‑100 (MQ=255)
                                                                                  tgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTAAGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGc                  >  1:1614549/4‑100 (MQ=38)
                                                                                    cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg                >  2:2778304/2‑100 (MQ=255)
                                                                                    cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg                >  1:2003636/2‑100 (MQ=255)
                                                                                    cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg                >  1:2765131/2‑100 (MQ=255)
                                                                                    aGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg                >  2:1502589/1‑100 (MQ=255)
                                                                                     gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACTTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGt               >  1:140823/1‑100 (MQ=255)
                                                                                     gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg                >  1:2438428/1‑99 (MQ=255)
                                                                                      ttCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTc              <  2:2779746/100‑1 (MQ=255)
                                                                                       tCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCt             >  1:2485895/1‑100 (MQ=255)
                                                                                        ccACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTg            >  2:2581760/1‑100 (MQ=255)
                                                                                         cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGATTACGCACGTTATACACGTCGTGGCGGACTgg           >  1:2167990/1‑100 (MQ=255)
                                                                                            gAACAGTGCGTGGATCGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACa        <  2:2652561/100‑1 (MQ=255)
                                                                                            gAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACa        <  1:1319467/100‑1 (MQ=255)
                                                                                             aaCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAt       <  2:1449875/100‑1 (MQ=255)
                                                                                              aCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGGTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAtt      <  1:1021398/100‑1 (MQ=255)
                                                                                               cAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTa     <  2:2662428/100‑1 (MQ=255)
                                                                                                aGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTaa    >  1:2410299/1‑100 (MQ=255)
                                                                                                 gTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAc   >  1:549745/1‑100 (MQ=255)
                                                                                                  tGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAcc  <  1:907/100‑1 (MQ=255)
                                                                                                  tGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAcc  >  2:1846638/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
GTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACC  >  NC_000913/2925943‑2926140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: