Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,774 (T)7→8 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7671.T98.4% 406.0 / ‑0.4 121coding (151/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (58/61);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 138 total aligned reads displayed.

GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTG  >  NC_000913/2809668‑2809859
                                                                                                    |                                                                                             
   tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttt                                                                                           >  2:1967925/1‑100 (MQ=255)
     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttt                                                                                         >  2:824955/1‑100 (MQ=255)
     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttt                                                                                         >  1:1479334/1‑100 (MQ=255)
     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCCGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttt                                                                                         >  2:2070385/1‑100 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttt                                                                                       <  2:544041/100‑1 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttt                                                                                       <  1:176879/100‑1 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttt                                                                                       <  1:1482460/100‑1 (MQ=255)
         gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttc                                                                                     >  2:1518608/1‑98 (MQ=255)
         gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttc                                                                                     >  1:3165347/1‑98 (MQ=255)
              tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                 >  2:3162054/1‑100 (MQ=255)
              tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                 <  1:2096720/100‑1 (MQ=255)
                tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGca                                                                               <  1:2123748/100‑1 (MQ=255)
                tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGca                                                                               <  2:813811/100‑1 (MQ=255)
                  ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTTcatc                                                                             >  2:501245/1‑100 (MQ=255)
                  ccGAGTGTTATTAGTTATATGCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatc                                                                             <  1:1237201/100‑1 (MQ=255)
                    gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatcat                                                                           >  2:2033512/1‑100 (MQ=255)
                       tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTa                                                                        <  2:1887440/100‑1 (MQ=255)
                        gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCATCTCGAAAGCGTTTTTTTTCTCCTTCATCATTTAt                                                                       >  2:1815491/1‑100 (MQ=255)
                         ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATg                                                                      >  2:1720042/1‑100 (MQ=255)
                           attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGc                                                                     <  1:2588495/99‑1 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGgcg                                                                >  1:1846141/1‑100 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGgcg                                                                >  1:113992/1‑100 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAggag                                                                <  1:2821625/100‑3 (MQ=255)
                                 tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                              <  1:840992/100‑1 (MQ=255)
                                   tatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAg                                                            >  2:382710/1‑100 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                           <  1:1987306/100‑1 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                           >  1:1223060/1‑100 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                           >  1:1173533/1‑100 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                           >  1:1705029/1‑100 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAggagaaaaa                                                           <  1:1233543/100‑8 (MQ=37)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                          <  2:2389110/100‑1 (MQ=255)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                          <  2:1543617/100‑1 (MQ=255)
                                      tCCGGTGGCCTTTGCGTTCGATCTGAATGCGACCCTTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTAAGCAGGCGCGAGcc                                                          <  2:621516/99‑1 (MQ=255)
                                       ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                        >  1:690007/1‑100 (MQ=255)
                                       ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                        <  2:699697/100‑1 (MQ=255)
                                       ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                        >  1:1752099/1‑100 (MQ=255)
                                       ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCAGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGACGCGAGCCAg                                                        >  2:2135419/1‑100 (MQ=255)
                                        cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                       <  1:1198526/100‑1 (MQ=255)
                                        cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                       >  2:2421886/1‑100 (MQ=255)
                                         ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCCGGCGCGAGCCAGtt                                                      >  2:2623935/1‑100 (MQ=255)
                                         ggTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGtt                                                      <  1:3163353/100‑1 (MQ=255)
                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                       <  2:3254655/97‑1 (MQ=255)
                                           tGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCg                                                    <  1:2770910/100‑1 (MQ=255)
                                            ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                   <  2:1318771/100‑1 (MQ=255)
                                             gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                  <  1:223047/100‑1 (MQ=255)
                                              ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                 >  2:692437/1‑100 (MQ=255)
                                              ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTGTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                 >  2:2082185/1‑100 (MQ=255)
                                              ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATGCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCa                                                 <  1:43707/100‑1 (MQ=255)
                                               cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                >  2:380759/1‑100 (MQ=255)
                                               cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                >  2:380397/1‑100 (MQ=255)
                                                  tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAACGCGTTTTTTTTCTCCTGAATCATTTATGCAGGAGCGAGCCAGTTCGTCATTa                                              >  2:1806904/1‑99 (MQ=255)
                                                     gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                          <  1:424480/100‑1 (MQ=255)
                                                      ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg                                         >  1:2882186/1‑100 (MQ=255)
                                                         ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                      >  1:809565/1‑100 (MQ=255)
                                                         ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                      <  1:1041844/100‑1 (MQ=255)
                                                          gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                     <  1:795648/100‑1 (MQ=255)
                                                            cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTTTTTCATTTATGCAGGCGCGAGCCAGTTTGTCATTACCGCGATGCTg                                   >  2:1161568/1‑100 (MQ=255)
                                                            cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                   <  1:2033537/100‑1 (MQ=255)
                                                            cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                   <  2:1823581/100‑1 (MQ=255)
                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                  >  1:1921397/1‑100 (MQ=255)
                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                  <  1:2441692/100‑1 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                 <  1:2123632/100‑1 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                 <  2:2560033/100‑1 (MQ=255)
                                                               aaTGCGACCGGTCGGGGTTTCTCTCCTCTGGAAGGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                   <  1:1143573/97‑1 (MQ=255)
                                                               aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                >  2:804132/1‑100 (MQ=255)
                                                                aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                               >  1:2609800/1‑100 (MQ=255)
                                                                aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                               >  1:2609780/1‑100 (MQ=255)
                                                                 tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                               >  1:344529/1‑99 (MQ=255)
                                                                    gtcccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCg                            <  2:2826316/97‑1 (MQ=255)
                                                                    gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                           >  1:1375848/1‑100 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                          <  1:2060603/100‑1 (MQ=255)
                                                                      cccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                          <  1:3201230/99‑1 (MQ=255)
                                                                      cccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                          <  1:1142350/99‑1 (MQ=255)
                                                                          tCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtag                     <  2:2547402/100‑1 (MQ=255)
                                                                          tCTGGGATTCTCTCCGCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtag                     <  2:1203082/100‑1 (MQ=255)
                                                                           cTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtagt                    <  1:103461/100‑1 (MQ=255)
                                                                             gggATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                  >  1:1637180/1‑100 (MQ=255)
                                                                             gggATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                  >  1:2860184/1‑100 (MQ=255)
                                                                             gggATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                  >  1:3123275/1‑100 (MQ=255)
                                                                                aTTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATGATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg               <  2:534210/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg              <  2:1921374/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg              <  1:10110/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg              <  1:159971/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg              <  1:2731229/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg              <  1:824964/100‑1 (MQ=255)
                                                                                  tctctcCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt            <  1:488362/100‑1 (MQ=255)
                                                                                   ctctcctctcGAAAGCGTTTTTTTTCTCTTGCATCATTTATGCAGGCGCAAGCCAGTTCGTCATTACCCCGATGCTGGCAGCCGGGAGTAGTTTGTgg              >  2:1125873/1‑98 (MQ=255)
                                                                                   ctctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGa             <  2:2797361/99‑1 (MQ=255)
                                                                                     ctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt           >  2:2002105/1‑99 (MQ=255)
                                                                                     ctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg          >  1:255675/1‑100 (MQ=255)
                                                                                     ctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg          >  2:1543967/1‑100 (MQ=255)
                                                                                      tcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc         <  1:854932/100‑1 (MQ=255)
                                                                                       cctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct        <  1:1518623/100‑1 (MQ=255)
                                                                                        ctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg       >  2:1371257/1‑100 (MQ=255)
                                                                                        ctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg       <  2:689998/100‑1 (MQ=255)
                                                                                         tctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTCGTTTGTGGTTtgctgc      >  2:36874/1‑100 (MQ=255)
                                                                                         tctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc      <  2:1705008/100‑1 (MQ=255)
                                                                                         tctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg       <  1:558965/99‑1 (MQ=255)
                                                                                           tcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc    >  1:1418480/1‑100 (MQ=255)
                                                                                            cGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt   >  1:720585/1‑100 (MQ=255)
                                                                                                    |                                                                                             
GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTG  >  NC_000913/2809668‑2809859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: