BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	94.9%	400.8 / 12.5	118	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (2/4); new base C (57/55); total (59/59)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGtt‑t                                                                                                    >  1:1047788/1‑100 (MQ=255)

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCACGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c                                                                                                 <  2:2191762/100‑1 (MQ=255)

   ncaGCGTAGTAAAAGAGACCGCCAGCGACTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c                                                                                                 >  2:1272043/2‑98 (MQ=255)

   acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑cc                                                                                              <  2:240321/100‑1 (MQ=255)

   acaGCGTAGTAAAAGAGACCACCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑cc                                                                                              <  1:2226518/100‑1 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑ccc                                                                                             >  2:3007166/1‑100 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑ccc                                                                                             >  1:2911774/1‑100 (MQ=255)

       cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGCCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑ccc‑c                                                                                           <  2:267065/98‑1 (MQ=255)

        gtagtaAAACAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTCATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑T‑CCC‑CCCg                                                                                        <  2:1601843/100‑1 (MQ=255)

          agtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑ccc‑ccccgc                                                                                      >  1:2122324/1‑97 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑c‑ccc‑ccccgcc                                                                                     >  2:2960615/1‑96 (MQ=255)

                    aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAAc                                                                             <  2:539001/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGccca                                                                              >  2:451741/1‑96 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAAcc                                                                            <  2:2525495/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAAcc                                                                            >  1:1051915/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAAcc                                                                            <  2:233615/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAAcc                                                                            >  1:2864035/1‑100 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAg                                                                        >  2:1862165/1‑100 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAg                                                                        <  2:428084/100‑1 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAg                                                                        <  1:1892142/100‑1 (MQ=255)

                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGt                                                                       <  1:261164/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGtt                                                                      <  2:2512027/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGtt                                                                      <  1:1405946/100‑1 (MQ=255)

                            cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGttt                                                                     <  2:1633601/100‑1 (MQ=255)

                             gTCTGGTTCTCTACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTa                                                                    <  1:1084557/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATc                                                                  <  1:541056/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATc                                                                  <  2:2445226/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATcc                                                                 >  1:1425198/1‑100 (MQ=255)

                                 tgTTCTCGACGCCCATTGTTGCTAATGGTCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCa                                                                <  1:309321/99‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCa                                                                >  1:210257/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCAt                                                               >  1:2800824/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCAt                                                               >  1:2334921/1‑100 (MQ=255)

                                   ttCTCGACGCTCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑CGCCT‑CCCGCCAGCGCCAACCATAGTTTATCCAtt                                                              <  2:2145831/100‑1 (MQ=255)

                                    tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCAttn                                                             <  2:3191930/100‑2 (MQ=255)

                                    tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTATCGTTAC‑‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCAttt                                                             <  2:536323/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  1:2973465/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2041562/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGAT‑T‑‑C‑CCCACCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            >  2:2290435/1‑100 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           >  1:1990204/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  2:2831108/1‑100 (MQ=255)

                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  1:1464432/1‑100 (MQ=255)

                                        gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                         <  1:1379890/100‑1 (MQ=255)

                                         aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTATCGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                        <  1:311598/100‑1 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                       >  2:3159804/1‑100 (MQ=255)

                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                       <  2:2095898/100‑1 (MQ=255)

                                           gCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCg                                                      <  1:1707196/100‑1 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     >  2:2317954/1‑100 (MQ=255)

                                                ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                 >  1:299819/1‑100 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               <  1:435494/100‑1 (MQ=255)

                                                   ttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAACTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTTCGCCCGGGTTTCa                                               >  2:2006727/1‑99 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCC‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            >  2:2796620/1‑100 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCAATTTGCGCCCGGGTTTCATCg                                            >  1:3170763/1‑99 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          <  1:2943059/100‑1 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                         >  2:1127197/1‑100 (MQ=255)

                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                         <  2:435729/100‑1 (MQ=255)

                                                         aTGGCCGATGGTTGATTTCTAACGCCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                        <  1:1692878/100‑1 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                      >  2:1023811/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:2928415/100‑1 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   >  2:810749/1‑100 (MQ=255)

                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                  <  1:2364787/100‑1 (MQ=255)

                                                                 tGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                <  1:1198714/100‑1 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               >  2:1275525/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  2:676706/100‑1 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              >  1:1252379/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              >  1:674757/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             >  1:2291770/1‑100 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             <  1:2349818/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                            <  1:628308/100‑1 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          >  2:1980185/1‑100 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          <  2:3042130/100‑1 (MQ=255)

                                                                       aTTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          <  2:3052121/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  2:63943/100‑1 (MQ=255)

                                                                           cTAACGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCCCAGAt                      >  2:367185/1‑100 (MQ=255)

                                                                             aaCGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    <  1:446276/100‑1 (MQ=255)

                                                                              aCGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                   <  2:2334894/100‑1 (MQ=255)

                                                                              aCGTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                   >  1:1168585/1‑100 (MQ=255)

                                                                                gTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 >  1:2759643/1‑100 (MQ=255)

                                                                                gTCGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 <  1:245275/100‑1 (MQ=255)

                                                                                  cGGTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  2:2847839/100‑1 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              >  1:1169672/1‑100 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              >  1:553129/1‑100 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              >  1:2034340/1‑100 (MQ=255)

                                                                                   ggTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga              >  1:1879261/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  2:2949075/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:1231823/100‑1 (MQ=255)

                                                                                    gTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  2:1329253/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  2:1168571/100‑1 (MQ=255)

                                                                                      gAATTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           >  2:1910896/1‑100 (MQ=255)

                                                                                        aTTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  1:436478/1‑100 (MQ=255)

                                                                                        aTTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:931711/100‑1 (MQ=255)

                                                                                        aTTTTCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:2571692/100‑1 (MQ=255)

                                                                                         ttttCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgan         <  1:1272057/99‑2 (MQ=255)

                                                                                         ttttCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           >  2:3150211/1‑97 (MQ=255)

                                                                                           ttCTCGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  2:1658831/1‑100 (MQ=255)

                                                                                             ctcGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGCAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  2:2203552/1‑100 (MQ=255)

                                                                                             ctcGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     >  2:383878/1‑99 (MQ=255)

                                                                                               cGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  2:210252/100‑1 (MQ=255)

                                                                                               cGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  2:1764193/1‑100 (MQ=255)

                                                                                               cGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCATGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  2:1545090/100‑1 (MQ=255)

                                                                                               cGTT‑TC‑C‑CCC‑CCCGCCAGCGCCAACCATAGTCTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  2:3239188/1‑100 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑C‑CCC‑CCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`