Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA33.5% 361.6 / 211.0 230intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (84/69);  new base A (71/6);  total (155/75)
Fisher's exact test for biased strand distribution p-value = 2.30e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01

Only 100 of 233 total aligned reads displayed.

GGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGC  >  NC_000913/3705873‑3706068
                                                                                                 |                                                                                                            
gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                            >  1:1916217/1‑100 (MQ=255)
  gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                           >  2:628601/1‑99 (MQ=255)
     tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                       >  2:703602/1‑100 (MQ=255)
      gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                      <  1:1120067/100‑1 (MQ=255)
        aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                    >  1:3186732/1‑100 (MQ=255)
           aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                 <  1:1311204/100‑1 (MQ=255)
             tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                               >  2:1408319/1‑100 (MQ=255)
              gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                              >  2:1467429/1‑100 (MQ=255)
               ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTGTGCCGGATGCGGCGTTAACGCCTTAt                                                                                             >  1:1380772/1‑100 (MQ=255)
               ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                             >  2:2130855/1‑100 (MQ=255)
                    ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                        <  2:1228278/100‑1 (MQ=255)
                    ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                        >  2:2765359/1‑100 (MQ=255)
                       tgtcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                     <  2:2597359/97‑1 (MQ=25)
                        ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                    <  2:37798/100‑1 (MQ=255)
                        ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                    <  2:34747/100‑1 (MQ=255)
                         ncgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                  >  2:2870104/2‑100 (MQ=255)
                         gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                  >  1:3175436/1‑100 (MQ=255)
                          cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                 >  1:1146424/1‑100 (MQ=255)
                           gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                >  1:834200/1‑100 (MQ=255)
                              gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                             >  2:1328782/1‑100 (MQ=255)
                               cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                            >  1:569584/1‑100 (MQ=255)
                               cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                            <  1:236809/100‑1 (MQ=255)
                                aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                           <  1:1218372/100‑1 (MQ=255)
                                 cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGTCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                          >  2:2685174/1‑100 (MQ=255)
                                 cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                          <  1:2906089/100‑1 (MQ=255)
                                  gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                         <  1:1050419/100‑1 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                        <  1:273720/100‑1 (MQ=255)
                                   cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                        >  2:1303335/1‑100 (MQ=255)
                                      agGCACAGCGCCACCGGGTATACAGAATGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                     >  1:1987533/1‑100 (MQ=255)
                                        gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                   <  1:2840855/100‑1 (MQ=255)
                                              cgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACa                                                             <  2:299161/100‑1 (MQ=255)
                                               gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                            <  1:2410621/100‑1 (MQ=255)
                                                       gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAt                                                <  2:1533940/100‑1 (MQ=255)
                                                           aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTc                                         <  2:2928411/100‑1 (MQ=255)
                                                            cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑g                                      >  2:1735310/1‑100 (MQ=255)
                                                             aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑Gt                                     >  1:879260/1‑100 (MQ=255)
                                                              gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTcn                                        <  2:464951/98‑2 (MQ=255)
                                                               aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCATGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAg                                   <  1:3094939/100‑1 (MQ=255)
                                                                 tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGc                                 >  1:2091747/1‑100 (MQ=255)
                                                                 tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGc                                 <  2:613185/100‑1 (MQ=255)
                                                                  gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGcc                                >  2:742067/1‑100 (MQ=255)
                                                                  gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGcc                                <  1:2433058/100‑1 (MQ=255)
                                                                  gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGcc                                <  1:3063285/100‑1 (MQ=255)
                                                                     gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAACATTGC‑‑AGCATT‑‑‑TTC‑‑GTAGGACt                               >  2:1344293/1‑98 (MQ=255)
                                                                       cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                           <  2:3244934/100‑1 (MQ=255)
                                                                       cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                           <  1:820931/100‑1 (MQ=255)
                                                                       cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                           <  2:1142706/100‑1 (MQ=255)
                                                                        aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATaa                          <  2:1583110/100‑1 (MQ=255)
                                                                          aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                        <  2:3245923/100‑1 (MQ=255)
                                                                          aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                        >  2:1105529/1‑100 (MQ=255)
                                                                           gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCg                       <  1:1566178/100‑1 (MQ=255)
                                                                           gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCg                       <  2:2172200/100‑1 (MQ=255)
                                                                            tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                      <  2:2390336/100‑1 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                     >  1:2766322/1‑100 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                     >  1:381876/1‑100 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                     <  1:1467443/100‑1 (MQ=255)
                                                                             gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                     >  2:1012508/1‑100 (MQ=255)
                                                                               gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                   <  1:703611/100‑1 (MQ=255)
                                                                                cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                  <  1:2770920/100‑1 (MQ=255)
                                                                                  ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGCTAAGCGGAGCGCa                <  2:2019973/100‑1 (MQ=255)
                                                                                   tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCAt               >  2:510925/8‑100 (MQ=18)
                                                                                   tGTTTATGCCGGATGGGGCGTTAGCGCCTTATCCGGTCTACAAGATCGTGCAAATTCGATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                <  2:254476/99‑1 (MQ=18)
                                                                                    ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATc              >  1:1181973/7‑100 (MQ=18)
                                                                                    ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATc              >  2:2345972/7‑100 (MQ=17)
                                                                                     tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa             <  1:1509871/100‑1 (MQ=255)
                                                                                     tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa             >  2:1826380/1‑100 (MQ=255)
                                                                                     tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATc              >  1:2237852/6‑99 (MQ=17)
                                                                                     cgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGT‑‑GGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa             >  2:2242991/6‑100 (MQ=21)
                                                                                      gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg            >  2:3022136/5‑100 (MQ=17)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGC‑‑AAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg            >  1:620308/5‑100 (MQ=17)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg            >  1:780444/5‑100 (MQ=17)
                                                                                      gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGC‑‑GGAGAA‑‑‑AAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg           >  2:1477032/5‑100 (MQ=18)
                                                                                       taggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGC‑‑AGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg           >  1:2269847/4‑100 (MQ=18)
                                                                                       taggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGC‑‑AGAGAT‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg           >  2:409248/4‑100 (MQ=11)
                                                                                       taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg           >  1:1747510/4‑100 (MQ=18)
                                                                                        atgtcGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc          <  1:2685208/96‑1 (MQ=255)
                                                                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  2:100649/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  2:1550216/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  1:246921/3‑100 (MQ=18)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  2:2428923/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGC‑‑AAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  1:2966014/3‑100 (MQ=17)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc          >  1:1668977/3‑100 (MQ=18)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:2180027/3‑100 (MQ=21)
                                                                                        aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGCGGAGAAAA‑‑‑T‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGct         >  2:1160865/3‑99 (MQ=18)
                                                                                        aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc          >  2:19963/1‑100 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:3200750/2‑100 (MQ=17)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  2:3169858/2‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:35164/2‑100 (MQ=21)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  1:625132/2‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGAC‑‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCa         >  1:2664858/2‑100 (MQ=18)
                                                                                         ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa         >  2:2262885/2‑100 (MQ=17)
                                                                                          gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt        >  1:2120521/1‑100 (MQ=255)
                                                                                          gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt        >  2:736298/1‑100 (MQ=255)
                                                                                          gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag        >  2:177402/1‑99 (MQ=18)
                                                                                          gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATT‑‑‑CTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGctg        >  1:1821118/1‑98 (MQ=18)
                                                                                          gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa        >  1:2633705/1‑99 (MQ=17)
                                                                                          gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGC‑‑AATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa         >  1:3137849/1‑100 (MQ=21)
                                                                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag        >  1:773671/1‑99 (MQ=18)
                                                                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag        >  1:1724615/1‑99 (MQ=18)
                                                                                               aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg   <  1:4128/100‑1 (MQ=255)
                                                                                                 |                                                                                                            
GGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGC  >  NC_000913/3705873‑3706068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: