Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9780AC30.1% 478.6 / 260.7 281intergenic (‑188/+120)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base A (89/83);  new base C (74/0);  total (198/83)
Fisher's exact test for biased strand distribution p-value = 3.33e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.69e-01

Only 100 of 283 total aligned reads displayed.

GAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCA  >  NC_000913/3705879‑3706076
                                                                                                   |                                                                                                             
gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                               <  1:1120067/100‑1 (MQ=255)
 naggaTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                              >  2:506530/5‑100 (MQ=255)
     aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                          <  1:2813743/100‑1 (MQ=255)
     aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                          <  1:985720/100‑1 (MQ=255)
     aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                                          <  1:1311204/100‑1 (MQ=255)
         ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                      >  2:2130855/1‑100 (MQ=255)
         ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                      >  1:1202138/1‑100 (MQ=255)
          tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                     >  1:651520/1‑100 (MQ=255)
              ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                 >  2:2765359/1‑100 (MQ=255)
              ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                 <  2:1228278/100‑1 (MQ=255)
                aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                               <  1:2708959/100‑3 (MQ=37)
                 tgtcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                              <  2:2597359/97‑1 (MQ=25)
                  ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                             <  1:2187116/100‑2 (MQ=25)
                   gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                            >  1:3175436/1‑100 (MQ=255)
                    cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                           >  1:1146424/1‑100 (MQ=255)
                    cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                           <  1:1932104/100‑1 (MQ=255)
                     gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                          >  1:834200/1‑100 (MQ=255)
                        gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                       >  2:1328782/1‑100 (MQ=255)
                        gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                       >  1:2452839/1‑100 (MQ=255)
                         cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                      >  1:569584/1‑100 (MQ=255)
                            gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                   <  1:1050419/100‑1 (MQ=255)
                             cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                  <  1:273720/100‑1 (MQ=255)
                             cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                  >  2:821864/1‑100 (MQ=255)
                                  gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                             <  1:402780/100‑1 (MQ=255)
                                  gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                             <  1:2840855/100‑1 (MQ=255)
                                   cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            <  2:1297053/100‑1 (MQ=255)
                                   cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                            >  2:1509856/1‑100 (MQ=255)
                                      aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                         <  1:1722279/100‑1 (MQ=255)
                                          ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                     >  1:2396575/1‑100 (MQ=255)
                                              cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                             <  2:315224/100‑1 (MQ=255)
                                              cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                             >  2:2963667/1‑100 (MQ=255)
                                              cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                             <  1:623907/100‑1 (MQ=255)
                                              cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                             >  2:738546/1‑100 (MQ=255)
                                                     aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTc                                                 <  2:1349152/100‑1 (MQ=255)
                                                      cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑g                                              >  2:1117587/1‑100 (MQ=255)
                                                       aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑Gt                                             >  2:3192613/1‑100 (MQ=255)
                                                       aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑Gt                                             >  1:879260/1‑100 (MQ=255)
                                                        gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTcn                                                <  2:464951/98‑2 (MQ=255)
                                                        gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTa                                            >  2:30064/1‑100 (MQ=255)
                                                         aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCATGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAg                                           <  1:3094939/100‑1 (MQ=255)
                                                           tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGc                                         <  2:613185/100‑1 (MQ=255)
                                                                 cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                   <  2:1142706/100‑1 (MQ=255)
                                                                 cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                   <  2:3244934/100‑1 (MQ=255)
                                                                 cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                   >  1:2090669/1‑100 (MQ=255)
                                                                  aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATaa                                  <  2:1583110/100‑1 (MQ=255)
                                                                     gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCg                               <  1:1566178/100‑1 (MQ=255)
                                                                      tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                              <  2:2390336/100‑1 (MQ=255)
                                                                       gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                             >  1:1339308/1‑100 (MQ=255)
                                                                       gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                             >  1:2766322/1‑100 (MQ=255)
                                                                        ngcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                             >  2:1417806/2‑99 (MQ=255)
                                                                          cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                          >  1:2258854/1‑100 (MQ=255)
                                                                           tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcgc                         <  1:2920300/100‑1 (MQ=255)
                                                                            ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGCTAAGCGGAGCGCa                        <  2:2019973/100‑1 (MQ=255)
                                                                             tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGC‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCAt                       >  2:1844463/8‑100 (MQ=17)
                                                                             tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCAt                       >  2:510925/8‑100 (MQ=18)
                                                                              ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATc                      >  2:2345972/7‑100 (MQ=17)
                                                                               tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                     <  1:725937/100‑1 (MQ=255)
                                                                               tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                    >  1:3003049/6‑100 (MQ=18)
                                                                               ngtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGC‑‑AGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                     >  2:696428/6‑100 (MQ=18)
                                                                               cgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGT‑‑GGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                     >  2:2242991/6‑100 (MQ=21)
                                                                                ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                    <  1:1408333/100‑1 (MQ=255)
                                                                                gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                    >  2:2899330/5‑100 (MQ=18)
                                                                                gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAACCGCGCAAATTCAATATATTGCGGAGAAAA‑‑‑T‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                   >  1:794244/5‑100 (MQ=18)
                                                                                 taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATTCT‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                   >  2:1472395/4‑100 (MQ=18)
                                                                                 taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                   >  1:2283585/4‑100 (MQ=17)
                                                                                 taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                   >  1:1747510/4‑100 (MQ=18)
                                                                                 taggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                   >  2:2439937/4‑100 (MQ=18)
                                                                                  aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  2:100649/3‑100 (MQ=17)
                                                                                  aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  2:1550083/3‑100 (MQ=17)
                                                                                  aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  1:1225830/3‑100 (MQ=17)
                                                                                  aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  1:1661117/3‑100 (MQ=21)
                                                                                  aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGC‑‑AATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcg                 >  2:2281968/3‑99 (MQ=18)
                                                                                  aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGCGGAGAAAA‑‑‑T‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGct                 >  2:1160865/3‑99 (MQ=18)
                                                                                  aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                  >  2:19963/1‑100 (MQ=255)
                                                                                  aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                  >  1:523481/1‑100 (MQ=255)
                                                                                   ggtcggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  2:2488120/11‑100 (MQ=18)
                                                                                   ggacggataaggcgTTCACGCCGCATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCC‑‑AGATAT‑‑‑CCT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                  >  1:2423221/11‑100 (MQ=25)
                                                                                   ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:3200750/2‑100 (MQ=17)
                                                                                   ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:35164/2‑100 (MQ=21)
                                                                                   ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:1470640/2‑100 (MQ=21)
                                                                                   ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:774328/2‑100 (MQ=17)
                                                                                   ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                 >  1:2661160/2‑100 (MQ=18)
                                                                                    gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                >  1:2120521/1‑100 (MQ=255)
                                                                                    gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                >  2:177402/1‑99 (MQ=18)
                                                                                    gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATT‑‑‑CTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGctg                >  1:1821118/1‑98 (MQ=18)
                                                                                    gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGC‑‑AATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                 >  1:3137849/1‑100 (MQ=21)
                                                                                     ccggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat               >  1:2537022/9‑98 (MQ=17)
                                                                                      cggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatt              >  2:2807920/8‑100 (MQ=17)
                                                                                       ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc            >  2:2689001/7‑98 (MQ=21)
                                                                                       ggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt             >  2:367496/7‑100 (MQ=18)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt             >  1:2153548/7‑100 (MQ=17)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct             >  1:375868/7‑96 (MQ=25)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat            >  2:3263145/7‑98 (MQ=25)
                                                                                       ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat            >  2:1733069/7‑98 (MQ=21)
                                                                                         aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg           <  2:381869/100‑1 (MQ=255)
                                                                                          taaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACT‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctttac          >  2:2160413/4‑93 (MQ=21)
                                                                                          taaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGC‑‑AGGAACG‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactacgt          >  1:3191400/4‑93 (MQ=25)
                                                                                            cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct        >  2:1045833/1‑100 (MQ=255)
                                                                                            aggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCATTtatgcgtt       >  1:1497246/2‑97 (MQ=25)
                                                                                                gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc    >  1:2679524/1‑100 (MQ=255)
                                                                                                   |                                                                                                             
GAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCA  >  NC_000913/3705879‑3706076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: