Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG41.6% 342.5 / inf 281intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (92/72);  new base G (113/4);  total (205/76)
Fisher's exact test for biased strand distribution p-value = 4.41e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-02

Only 100 of 283 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTC  >  NC_000913/3705885‑3706082
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    tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                        >  1:651520/1‑100 (MQ=255)
      ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                      >  1:1213236/1‑100 (MQ=255)
       cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                     >  1:1660194/1‑100 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                    <  2:1228278/100‑1 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                    >  2:2765359/1‑100 (MQ=255)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                                  <  1:3096409/100‑1 (MQ=255)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                                  <  1:2708959/100‑3 (MQ=37)
           tgtcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATa                                                                                                 <  2:2597359/97‑1 (MQ=25)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                                <  2:34747/100‑1 (MQ=255)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                                <  2:37798/100‑1 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                         >  1:2452839/1‑100 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                         >  2:1328782/1‑100 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                        >  1:569584/1‑100 (MQ=255)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                     <  1:1050419/100‑1 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                    >  2:821864/1‑100 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                    <  1:273720/100‑1 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                    >  2:1303335/1‑100 (MQ=255)
                          agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                                 <  2:3211284/100‑1 (MQ=255)
                           ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                                >  1:1744159/1‑100 (MQ=255)
                             cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                              >  2:1509856/1‑100 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            >  1:1178742/1‑100 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                           <  1:1722279/100‑1 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                                >  2:2963667/1‑100 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                                <  1:623907/100‑1 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                                >  2:738546/1‑100 (MQ=255)
                                           gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAt                                                            >  1:2019997/1‑100 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAtttt                                                        <  2:1008930/100‑1 (MQ=255)
                                               aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTc                                                       <  2:1349152/100‑1 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                                    >  2:1117587/1‑100 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTa                                                  >  2:30064/1‑100 (MQ=255)
                                                     tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGc                                               <  2:613185/100‑1 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGcc                                              <  1:3063285/100‑1 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTg                                            >  2:2186363/1‑100 (MQ=255)
                                                           cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATa                                         <  2:1142706/100‑1 (MQ=255)
                                                            aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATaa                                        <  2:1583110/100‑1 (MQ=255)
                                                              aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGc                                      <  2:3245923/100‑1 (MQ=255)
                                                               gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCg                                     <  1:1566178/100‑1 (MQ=255)
                                                               gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCg                                     >  2:4128/1‑100 (MQ=255)
                                                                 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGa                                   >  1:1339308/1‑100 (MQ=255)
                                                                 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGa                                   >  1:2766322/1‑100 (MQ=255)
                                                                  ngcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGa                                   >  2:1417806/2‑99 (MQ=255)
                                                                  cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                                  <  1:2900975/100‑1 (MQ=255)
                                                                      ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGCTAAGCGGAGCGCa                              <  2:2019973/100‑1 (MQ=255)
                                                                       tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCAt                             >  2:1844463/8‑100 (MQ=17)
                                                                       tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCAt                             >  2:510925/8‑100 (MQ=18)
                                                                         tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                           <  1:725937/100‑1 (MQ=255)
                                                                         tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATc                            >  1:2237852/6‑99 (MQ=17)
                                                                         tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                           >  1:3166879/6‑100 (MQ=17)
                                                                         ngtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGC‑‑AGAAATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                           >  2:696428/6‑100 (MQ=18)
                                                                          ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                          >  1:2099995/1‑100 (MQ=255)
                                                                          ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                          <  1:1408333/100‑1 (MQ=255)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                          >  2:2899330/5‑100 (MQ=18)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                          >  1:786826/5‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATTCTCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                         >  2:1472395/4‑100 (MQ=18)
                                                                           tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                         <  1:2383699/100‑1 (MQ=255)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:1550216/3‑100 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  1:246921/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  2:2428923/3‑100 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGC‑‑AAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                        >  1:2966014/3‑100 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:2180027/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGCGGAGAAAAT‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGct                       >  2:1160865/3‑99 (MQ=18)
                                                                            aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                        >  1:523481/1‑100 (MQ=255)
                                                                            aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                        >  2:19963/1‑100 (MQ=255)
                                                                             tGCCGGATGCGGCGTTAACGCCTTATCTGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                       >  2:2147614/1‑100 (MQ=255)
                                                                             ggtcggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:2488120/11‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGct                       >  2:2320974/2‑99 (MQ=18)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:3200750/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:35164/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:1470640/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:774328/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:2661160/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  1:827925/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGC‑‑AGGAAACAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCa                       >  1:180380/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                       >  2:2262885/2‑100 (MQ=17)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAACCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                      >  2:177402/1‑99 (MQ=18)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                      >  1:2412234/1‑99 (MQ=17)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                      >  1:862/1‑99 (MQ=21)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                     >  1:2537022/9‑98 (MQ=17)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATACGCGTAGCGAATCAGGCa                       >  1:915406/9‑98 (MQ=17)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAATCCTTGCGAATTCAATATATTGT‑‑ATGGAAATTC‑‑‑‑AGGCCTGATAAGCGTAGCGCATCAGGCtttt                    >  2:479706/9‑100 (MQ=25)
                                                                               caggataaggcgTTCACGCCGCATCCTGCCTACAAAAGCACGCAAATTCAATATATTGC‑‑AGAGATGGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                     >  1:1884441/9‑100 (MQ=14)
                                                                                cggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttg                   >  1:2278680/8‑99 (MQ=21)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagct                    >  1:1239394/8‑97 (MQ=18)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatt                    >  2:2807920/8‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                  >  2:2689001/7‑98 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                   >  1:2153548/7‑100 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                   >  1:375868/7‑96 (MQ=25)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                   >  1:2827292/7‑96 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                  >  2:1733069/7‑98 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCATACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                   >  1:1138195/7‑100 (MQ=18)
                                                                                   aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                 >  2:2352674/1‑100 (MQ=255)
                                                                                   aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                 <  1:4128/100‑1 (MQ=255)
                                                                                    taaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGC‑‑AGGAACGAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactacgt                >  1:3191400/4‑93 (MQ=25)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct              <  2:3175399/100‑1 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct              >  2:1045833/1‑100 (MQ=255)
                                                                                      aggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCATTtatgcgtt             >  1:1497246/2‑97 (MQ=25)
                                                                                         cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg           <  2:2120495/100‑1 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc          >  1:2679524/1‑100 (MQ=255)
                                                                                                gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGTGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt   >  2:1645383/1‑100 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt   >  2:3090048/1‑100 (MQ=255)
                                                                                                   |                                                                                                          
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTC  >  NC_000913/3705885‑3706082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: