Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9850TC41.6% 273.5 / inf 281intergenic (‑195/+113)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (92/72);  new base C (113/4);  total (205/76)
Fisher's exact test for biased strand distribution p-value = 4.41e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-02

Only 100 of 283 total aligned reads displayed.

TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705886‑3706084
                                                                                                   |                                                                                                               
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                                >  1:2683104/1‑100 (MQ=255)
  ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTGTGCCGGATGCGGCGTTAACGCCTTAt                                                                                                               >  1:1380772/1‑100 (MQ=255)
  ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                                               >  2:2130855/1‑100 (MQ=255)
    gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                             >  2:1568755/1‑100 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                         <  2:2060411/100‑2 (MQ=37)
         aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                                        >  2:1633559/1‑100 (MQ=255)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                                       >  2:1334344/1‑100 (MQ=255)
           ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                                      <  2:37798/100‑1 (MQ=255)
           ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                                      <  2:34747/100‑1 (MQ=255)
           ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                                      <  1:2187116/100‑2 (MQ=25)
            gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                                    >  1:3175436/1‑100 (MQ=255)
             cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                                   <  1:1932104/100‑1 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                                  >  1:834200/1‑100 (MQ=255)
                 gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                               >  2:2965499/1‑100 (MQ=255)
                   aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                                             <  1:1218372/100‑1 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                            <  2:128274/100‑1 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                            <  1:2906089/100‑1 (MQ=255)
                     gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                           <  1:1050419/100‑1 (MQ=255)
                      cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                          >  2:821864/1‑100 (MQ=255)
                      cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGGTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                          >  2:1303478/1‑100 (MQ=255)
                       agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaa                                                                                         >  1:999389/1‑100 (MQ=255)
                          ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                                      >  1:3233446/1‑100 (MQ=255)
                           gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                                     <  1:2840855/100‑1 (MQ=255)
                                   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                             >  1:2396575/1‑100 (MQ=255)
                                       cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                                     <  2:315224/100‑1 (MQ=255)
                                       cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                                     >  2:738546/1‑100 (MQ=255)
                                          gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAt                                                                 <  2:1533940/100‑1 (MQ=255)
                                          gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAt                                                                 <  2:2802759/100‑1 (MQ=255)
                                               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑g                                                      >  2:1735310/1‑100 (MQ=255)
                                                aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑Gt                                                     <  1:662644/100‑1 (MQ=255)
                                                aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑Gt                                                     >  2:3192613/1‑100 (MQ=255)
                                                aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑Gt                                                     >  1:879260/1‑100 (MQ=255)
                                                    tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGc                                                 <  2:613185/100‑1 (MQ=255)
                                                      ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGccn                                               <  2:1170729/100‑2 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                           >  1:2090669/1‑100 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                           <  2:1065198/100‑1 (MQ=255)
                                                             aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                                        <  2:3245923/100‑1 (MQ=255)
                                                              gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                       >  2:4128/1‑100 (MQ=255)
                                                              gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                       >  1:589677/1‑100 (MQ=255)
                                                               tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCgg                                      <  2:2390336/100‑1 (MQ=255)
                                                                gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                                     >  2:1012508/1‑100 (MQ=255)
                                                                gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGa                                     >  1:381876/1‑100 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                                   <  1:2996701/100‑1 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                                   >  1:2632174/1‑100 (MQ=255)
                                                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                  >  1:2258854/1‑100 (MQ=255)
                                                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                  <  1:2770920/100‑1 (MQ=255)
                                                                      tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCAt                               >  2:510925/8‑100 (MQ=18)
                                                                      tGTTTATGCCGGATGGGGCGTTAGCGCCTTATCCGGTCTACAAGATCGTGCAAATTCGATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                                <  2:254476/99‑1 (MQ=18)
                                                                       ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                             >  2:1121180/7‑100 (MQ=18)
                                                                       ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATc                              >  1:1181973/7‑100 (MQ=18)
                                                                       ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATc                              >  2:2345972/7‑100 (MQ=17)
                                                                        tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                             <  1:1509871/100‑1 (MQ=255)
                                                                        tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                             <  1:2765394/100‑1 (MQ=255)
                                                                        ngtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGC‑‑AGAAAT‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                             >  2:696428/6‑100 (MQ=18)
                                                                        cgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGT‑‑GGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                             >  2:2242991/6‑100 (MQ=21)
                                                                         gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑ATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                            >  2:3022136/5‑100 (MQ=17)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                            >  1:780444/5‑100 (MQ=17)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAACCGCGCAAATTCAATATATTGCGGAGAAAA‑‑‑T‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                           >  1:794244/5‑100 (MQ=18)
                                                                         gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGC‑‑GGAGAA‑‑‑AAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                           >  2:1477032/5‑100 (MQ=18)
                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                           >  1:2283585/4‑100 (MQ=17)
                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                           >  1:1747510/4‑100 (MQ=18)
                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAACCGCGCAAATTCAATATATTGCGGAGAAAA‑‑‑T‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  2:200442/4‑100 (MQ=18)
                                                                          taggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                           >  2:2439937/4‑100 (MQ=18)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  1:1661117/3‑100 (MQ=21)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACT‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGc                          >  2:2708847/3‑100 (MQ=18)
                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGC‑‑AATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcg                         >  2:2281968/3‑99 (MQ=18)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑GTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                         <  1:1144524/100‑1 (MQ=255)
                                                                            ggacggataaggcgTTCACGCCGCATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCC‑‑AGATAT‑‑‑CCT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                          >  1:2423221/11‑100 (MQ=25)
                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  2:2650298/2‑100 (MQ=17)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:3195863/2‑100 (MQ=18)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:2169263/2‑100 (MQ=17)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                         >  2:1777595/2‑100 (MQ=21)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                         >  1:625132/2‑100 (MQ=18)
                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGAC‑‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCa                         >  1:2664858/2‑100 (MQ=18)
                                                                             gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                        >  1:2120521/1‑100 (MQ=255)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑AATT‑‑‑CTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGctg                        >  1:1821118/1‑98 (MQ=18)
                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGC‑‑AATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                         >  1:3137849/1‑100 (MQ=21)
                                                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGC‑‑AGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                        >  1:1724615/1‑99 (MQ=18)
                                                                             gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGAC‑‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCag                        >  1:3089461/1‑99 (MQ=18)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                       >  1:1253716/9‑98 (MQ=18)
                                                                              ccggataaggcgTTCACGCCGCATCCGGCCTACAAATCCTTGCGAATTCAATATATTGT‑‑ATGGAAA‑‑‑TTC‑‑‑‑AGGCCTGATAAGCGTAGCGCATCAGGCtttt                      >  2:479706/9‑100 (MQ=25)
                                                                              ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                       >  1:2720228/1‑100 (MQ=255)
                                                                              ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                       >  2:1371519/1‑98 (MQ=18)
                                                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGC‑‑AGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:2919671/7‑100 (MQ=18)
                                                                                ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  1:1488497/7‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGC‑‑ATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                     >  2:2831307/7‑100 (MQ=17)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattc                     >  2:184925/7‑96 (MQ=21)
                                                                                ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGC‑‑AGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                     >  2:1425824/7‑100 (MQ=17)
                                                                                ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                     >  1:497200/1‑100 (MQ=255)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                   <  2:1159230/100‑1 (MQ=255)
                                                                                   taaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACT‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctttac                  >  2:2160413/4‑93 (MQ=21)
                                                                                     cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                <  1:53518/100‑1 (MQ=255)
                                                                                         gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc            <  1:367114/100‑1 (MQ=255)
                                                                                          ttAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc           <  1:1448762/100‑1 (MQ=255)
                                                                                           tAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa          <  1:1576267/100‑1 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc        >  1:725969/1‑100 (MQ=255)
                                                                                             aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc        >  2:1697269/1‑100 (MQ=255)
                                                                                              cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa       >  1:2512069/1‑100 (MQ=255)
                                                                                                 cTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc    >  1:2461578/1‑100 (MQ=255)
                                                                                                   tATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCtt  >  2:3223255/1‑100 (MQ=255)
                                                                                                   |                                                                                                               
TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTT  >  NC_000913/3705886‑3706084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: