Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,706,0170CT30.2% 441.1 / 199.2 276intergenic (‑227/+81)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (101/77);  new base T (77/0);  total (199/77)
Fisher's exact test for biased strand distribution p-value = 2.04e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 278 total aligned reads displayed.

CGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTT  >  NC_000913/3705919‑3706116
                                                                                                  |                                                                                                       
 gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                                                       <  1:2410621/100‑1 (MQ=255)
      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑g                                                                                                 <  2:315224/100‑1 (MQ=255)
         gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAt                                                                                              <  2:1533940/100‑1 (MQ=255)
            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAt‑ttt                                                                                          <  2:1008930/100‑1 (MQ=255)
             aCAGATTGTTGACAAAGTTCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTc                                                                                         >  2:369323/1‑100 (MQ=255)
                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTcn                                                                                        <  2:464951/98‑2 (MQ=255)
                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTa                                                                                    >  2:30064/1‑100 (MQ=255)
                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGc                                                                                 >  1:2091747/1‑100 (MQ=255)
                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGcc                                                                                <  1:3063285/100‑1 (MQ=255)
                    gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGcc                                                                                <  1:2433058/100‑1 (MQ=255)
                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTg                                                                              >  2:2143141/1‑100 (MQ=255)
                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAACATTGCA‑GCAT‑TTTC‑‑GTAGGACt                                                                               >  2:1344293/1‑98 (MQ=255)
                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATa                                                                           <  2:1142706/100‑1 (MQ=255)
                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATa                                                                           <  1:820931/100‑1 (MQ=255)
                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATa                                                                           <  2:1065198/100‑1 (MQ=255)
                            aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGc                                                                        <  2:3245923/100‑1 (MQ=255)
                             gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCg                                                                       >  1:589677/1‑100 (MQ=255)
                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCgg                                                                      <  2:2390336/100‑1 (MQ=255)
                               gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGa                                                                     >  1:2766322/1‑100 (MQ=255)
                               gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGa                                                                     <  1:1467443/100‑1 (MQ=255)
                                cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAg                                                                    <  1:2900975/100‑1 (MQ=255)
                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAgc                                                                   <  1:703611/100‑1 (MQ=255)
                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAgc                                                                   >  1:2632174/1‑100 (MQ=255)
                                     tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCA‑TGGG‑CCAT‑‑GTAGGCCTGATAAGCGTAGCGCAt                                                               >  2:1844463/8‑100 (MQ=17)
                                     tgtgTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                                                               >  1:2206763/1‑100 (MQ=18)
                                      ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:1121180/7‑100 (MQ=18)
                                       tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCA‑GGAT‑ACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:2710195/6‑100 (MQ=17)
                                       ngtaggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCA‑GAAA‑TCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:696428/6‑100 (MQ=18)
                                       cgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGTG‑GGCT‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                             >  2:2242991/6‑100 (MQ=21)
                                        ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                                                            <  1:1408333/100‑1 (MQ=255)
                                        gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCA‑GAAA‑CCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                            >  2:2899330/5‑100 (MQ=18)
                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCA‑GAGA‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                            >  1:780444/5‑100 (MQ=17)
                                         taggCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCA‑GAAA‑TCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                           >  1:2269847/4‑100 (MQ=18)
                                         taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCA‑ATTC‑TCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                           >  2:1472395/4‑100 (MQ=18)
                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                                                           <  1:2383699/100‑1 (MQ=255)
                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TGAG‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                          >  2:2428923/3‑100 (MQ=17)
                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCA‑CCAT‑CCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                          >  1:1661117/3‑100 (MQ=21)
                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCA‑AGAT‑TTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                          >  1:2966014/3‑100 (MQ=17)
                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCA‑‑ATA‑TGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcg                                                         >  2:2281968/3‑99 (MQ=18)
                                          aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                                                          >  2:19963/1‑100 (MQ=255)
                                           tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TGTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCa                                                         <  1:1144524/100‑1 (MQ=255)
                                           ggtcggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑‑AAT‑CTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  2:2488120/11‑100 (MQ=18)
                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑‑AAT‑TCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGct                                                         >  2:2320974/2‑99 (MQ=18)
                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCA‑GAGT‑TCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  2:2650298/2‑100 (MQ=17)
                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCA‑ATCT‑TCTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCa                                                         >  2:1481007/2‑100 (MQ=18)
                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TGAG‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  1:2169263/2‑100 (MQ=17)
                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGAT‑CGAT‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  1:35164/2‑100 (MQ=21)
                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCA‑GGAG‑CTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  1:1549417/2‑100 (MQ=18)
                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑‑AAT‑CTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                         >  1:625132/2‑100 (MQ=18)
                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCA‑GGGA‑CTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCa                                                         >  1:2664858/2‑100 (MQ=18)
                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCA‑GAAA‑CCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                                                        >  2:177402/1‑99 (MQ=18)
                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑‑AAT‑TCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGctg                                                        >  1:1821118/1‑98 (MQ=18)
                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCA‑CCAT‑CCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                        >  1:862/1‑99 (MQ=21)
                                             ccggataaggcgTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt                                                      >  1:3198703/9‑100 (MQ=18)
                                             ccggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TGAG‑CCAC‑‑GTAGGCCTGATACGCGTAGCGAATCAGGCa                                                         >  1:915406/9‑98 (MQ=17)
                                             ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑TAT‑‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagc                                                       >  1:1490509/9‑98 (MQ=18)
                                             caggataaggcgTTCACGCCGCATCCTGCCTACAAAAGCACGCAAATTCAATATATTGCA‑GAGA‑TGGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                       >  1:1884441/9‑100 (MQ=14)
                                              cggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCA‑GGGA‑CTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagct                                                      >  1:1239394/8‑97 (MQ=18)
                                               ggataaggcgTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                                                    >  2:2689001/7‑98 (MQ=21)
                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCA‑TGAG‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                     >  2:2831307/7‑100 (MQ=17)
                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCA‑GTTT‑ATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                                                     >  1:375868/7‑96 (MQ=25)
                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCA‑GGGA‑CTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                                                     >  1:2731792/7‑96 (MQ=18)
                                               ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCA‑GGGA‑CTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                                                     >  1:2827292/7‑96 (MQ=21)
                                               ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAATCATGCAAATCCAAAATATTGCA‑GGAG‑TTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                                                    >  2:1733069/7‑98 (MQ=21)
                                                nataaggcgTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑CAT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAatttt                                                    >  2:116/6‑100 (MQ=18)
                                                gataaggcgTTTACGCCGCATCCGGCCTACAAATTC‑TGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttttcg                                                   >  2:510506/6‑98 (MQ=18)
                                                gataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCA‑GGAA‑CGAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactac                                                    >  2:1185122/6‑95 (MQ=25)
                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                                                   <  2:381869/100‑1 (MQ=255)
                                                  taaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑TAT‑‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctttac                                                  >  2:2160413/4‑93 (MQ=21)
                                                  taaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAAC‑GAT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaactacgt                                                  >  1:3191400/4‑93 (MQ=25)
                                                  tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                                                  >  2:618983/1‑100 (MQ=255)
                                                   aaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                                                 >  2:3083165/3‑100 (MQ=255)
                                                    cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                                <  2:3175399/100‑1 (MQ=255)
                                                    cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct                                                <  1:53518/100‑1 (MQ=255)
                                                       cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg                                             <  1:212371/100‑1 (MQ=255)
                                                        gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                            >  1:2679524/1‑100 (MQ=255)
                                                          tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa                                          <  2:834190/100‑1 (MQ=255)
                                                            aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc                                        >  1:725969/1‑100 (MQ=255)
                                                             cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa                                       >  1:2512069/1‑100 (MQ=255)
                                                              gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAG‑TGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt                                     >  2:1645383/1‑100 (MQ=255)
                                                               ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg                                      >  2:2112701/1‑99 (MQ=255)
                                                                   aTCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTg                                 <  1:1334358/100‑1 (MQ=255)
                                                                     ccGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                               <  1:47441/100‑1 (MQ=255)
                                                                      cGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTAt                              <  2:561470/100‑1 (MQ=255)
                                                                        gCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATgg                            >  2:1573372/1‑100 (MQ=255)
                                                                          cTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                          >  1:1767358/1‑100 (MQ=255)
                                                                             cAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                       >  2:793566/1‑100 (MQ=255)
                                                                             cAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                       >  2:1376224/1‑100 (MQ=255)
                                                                              aaaaTCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGTATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTa                      <  1:2301231/100‑1 (MQ=255)
                                                                              aaaaTCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTa                      >  2:738477/1‑100 (MQ=255)
                                                                               aaaTCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTaa                     >  2:2767227/1‑100 (MQ=255)
                                                                                  tCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGACTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatta                  <  1:3105679/100‑1 (MQ=255)
                                                                                   cGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAattat                 >  1:172448/1‑100 (MQ=255)
                                                                                     tGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc               >  1:2003669/1‑100 (MQ=255)
                                                                                      gCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg              <  1:1117601/100‑1 (MQ=255)
                                                                                       cAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa             >  1:1573585/1‑100 (MQ=255)
                                                                                       cAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGa             >  1:2022323/1‑100 (MQ=255)
                                                                                             cAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga       >  2:3222229/1‑100 (MQ=255)
                                                                                               aTACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGCGCGCATCAGGCATTTTTGCCTTTGCCATCCTTCTTCTATCGCTTTTCATTATTCGAtcac         >  2:2948839/1‑93 (MQ=25)
                                                                                                 aCATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGtt   >  1:426056/1‑100 (MQ=255)
                                                                                                  |                                                                                                       
CGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA‑GAAT‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTT  >  NC_000913/3705919‑3706116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑