Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T26.8% 365.4 / 203.6 168intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (57/66);  new base T (20/25);  total (77/91)
Fisher's exact test for biased strand distribution p-value = 8.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 174 total aligned reads displayed.

ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520405‑4520603
                                                                                                    |                                                                                                   
aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATg                                                                                                      >  1:2763160/1‑100 (MQ=255)
 tGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑c                                                                                                    >  1:1627304/1‑100 (MQ=255)
  gATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑c                                                                                                    >  1:242748/1‑99 (MQ=255)
  gATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑Ct                                                                                                   >  2:3141549/1‑100 (MQ=255)
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACTAGCATACCATTTGAGCTATCGCCCGATGTTCCCCCATACTGTAAAGCCGGAAACATG‑CTc                                                                                                  >  2:2941516/1‑100 (MQ=255)
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTc                                                                                                  >  1:203171/1‑100 (MQ=255)
      ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCgg                                                                                               <  2:2026343/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                              <  2:353880/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                              <  1:2173681/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGc                                                                                              <  1:2795009/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc                                                                                              <  1:1328265/100‑1 (MQ=39)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAACGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGc                                                                                              <  1:1392644/100‑1 (MQ=38)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGcn                                                                                             <  1:876731/100‑2 (MQ=38)
        ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCt                                                                                             <  2:2575497/100‑1 (MQ=38)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCGCCATACTGTAAAGCCGGAGACATG‑CTCCGGCtt                                                                                            <  2:2156940/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCtt                                                                                            <  1:3013336/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                            <  1:2970069/100‑1 (MQ=38)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                            <  1:3227716/100‑1 (MQ=38)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                            <  1:2395040/100‑1 (MQ=38)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACTTG‑CTCCGGCttt                                                                                           <  1:2147360/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCttt                                                                                           <  2:3040715/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCttt                                                                                           <  2:2621998/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCttt                                                                                           <  1:1057660/100‑1 (MQ=38)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCtt                                                                                            <  1:500015/99‑1 (MQ=38)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTa                                                                                          <  2:2388699/100‑1 (MQ=255)
           cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTa                                                                                          <  2:2323767/100‑1 (MQ=38)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAc                                                                                         <  1:420193/100‑1 (MQ=38)
            aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTAc                                                                                         <  1:1774110/100‑1 (MQ=38)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACa                                                                                        >  1:1774333/1‑100 (MQ=255)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACa                                                                                        <  1:1375674/100‑1 (MQ=38)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACa                                                                                        <  2:939395/100‑1 (MQ=38)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACa                                                                                        <  2:688051/100‑1 (MQ=38)
              atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAg                                                                                       <  1:1941959/100‑1 (MQ=38)
                atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTa                                                                                     >  2:1979050/1‑100 (MQ=255)
                  tttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTAtg                                                                                   <  1:360202/100‑2 (MQ=37)
                   ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTa                                                                                  >  2:721036/1‑100 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgag                                                                             <  1:3129207/100‑1 (MQ=255)
                         ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTgaga                                                                            <  2:1399840/100‑1 (MQ=255)
                          tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGaa                                                                           >  1:64707/1‑100 (MQ=255)
                           ttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAAt                                                                          >  1:234060/1‑100 (MQ=255)
                            tACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATa                                                                         >  2:2656992/1‑100 (MQ=255)
                             aCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATac                                                                        >  2:2717885/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                       <  1:2660404/100‑1 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                      <  2:64705/100‑1 (MQ=255)
                               aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                      <  2:3178852/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     <  2:204042/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  1:910057/1‑100 (MQ=255)
                                 gAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                    >  2:1193814/1‑100 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                   <  2:175784/100‑1 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                   <  2:185638/100‑1 (MQ=255)
                                   aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCAt                                                                  <  2:2844204/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              >  1:1541891/1‑100 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              <  1:2554318/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                              <  1:1160137/100‑1 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       >  1:882359/1‑100 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       <  1:1497374/100‑1 (MQ=255)
                                                 ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                    >  2:810798/1‑100 (MQ=255)
                                                   aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                  >  1:2525610/1‑100 (MQ=255)
                                                   aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                  >  2:2619468/1‑100 (MQ=255)
                                                   aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAACCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                  <  2:2500557/100‑1 (MQ=255)
                                                      tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                               >  1:48258/1‑100 (MQ=255)
                                                       gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                              <  2:2608463/100‑1 (MQ=255)
                                                        aGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaa                                             <  2:1479431/100‑1 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                            >  1:2609581/1‑100 (MQ=255)
                                                           gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                          >  1:3141257/1‑100 (MQ=255)
                                                            aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGCATACACCCATCAAGCATCATCGAAGGTTCAat                                            >  2:2819878/1‑97 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       <  1:595823/100‑1 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  1:1442005/1‑100 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  2:1267162/1‑100 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  2:1048267/1‑100 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  2:457231/1‑100 (MQ=255)
                                                                cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGccc                                     <  1:281712/100‑1 (MQ=255)
                                                                     tGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                <  2:904300/100‑1 (MQ=255)
                                                                      gTTCCCCCATACTGTAAAGCCGGAGACATG‑TTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                               <  2:879682/100‑1 (MQ=255)
                                                                      gTTCCCCCATACGGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGTGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                               <  1:2960798/100‑1 (MQ=255)
                                                                         cagtaaTACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:2916041/6‑100 (MQ=25)
                                                                          agtaaTACTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTCAATATCGCCCCTTTTTGATc                           >  2:571419/5‑100 (MQ=25)
                                                                           cccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          >  1:2025795/1‑100 (MQ=255)
                                                                            ccATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGc                         >  1:2241356/1‑100 (MQ=255)
                                                                             cATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATACTCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                        <  2:1958521/100‑1 (MQ=255)
                                                                                aCTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                     >  1:2327750/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCg                    >  1:2001276/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  1:2736935/1‑100 (MQ=38)
                                                                                  tGTAAAGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTTTTCGCTAGCGa                   >  2:2683980/1‑100 (MQ=37)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGCAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  2:1620046/1‑98 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  1:1455129/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  1:806057/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  2:2529375/1‑100 (MQ=38)
                                                                                     aaaGCCGGAG‑CATGTCTCCGCCTTTACAGTATTACTGAGAATACACCCATCAATCCTAATCGAAGGTTCAATATCGCCCCTTTTTGATCTCCAGCGCTTc                >  2:3001008/1‑100 (MQ=37)
                                                                                      aaGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  1:464480/1‑100 (MQ=255)
                                                                                      aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  2:1101683/1‑100 (MQ=38)
                                                                                      aaGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg               >  2:1149448/1‑100 (MQ=38)
                                                                                       aGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt              >  1:721097/1‑100 (MQ=255)
                                                                                       aGCCGGAG‑CATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGt              >  2:1757730/1‑100 (MQ=38)
                                                                                        gCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc             <  2:1802154/100‑1 (MQ=255)
                                                                                            gagaCATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGTAGGTTCAATGTCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg         <  1:2238430/100‑1 (MQ=255)
                                                                                                cATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     >  2:2742270/1‑100 (MQ=255)
                                                                                                  tG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGa   >  1:1094446/1‑100 (MQ=255)
                                                                                                   g‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc  >  1:685564/1‑100 (MQ=255)
                                                                                                   g‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc  >  2:978995/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATG‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520405‑4520603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑