Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,9580GC23.5% 85.9 / 21.0 34T297S (ACC→AGC) stfQQin prophage; putative side tail fibre assembly protein
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base C (0/8);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 4.52e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01

CATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGA  >  NC_000913/1634871‑1635055
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cATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgc                                                                                       <  1:1196553/100‑6 (MQ=1)
cATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgc                                                                                       <  1:2568546/100‑6 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                      <  2:2339454/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                      <  2:2339395/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                      <  1:2788028/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                      <  1:2788642/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                      <  1:551638/100‑7 (MQ=1)
  ttCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcgg                                                                                     <  2:2646583/100‑8 (MQ=1)
                atacAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGc                                                                       >  1:1591470/4‑100 (MQ=1)
                                                         gtgtTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCAt                              >  1:879965/1‑100 (MQ=35)
                                                          tgtTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATg                             >  2:1968450/1‑100 (MQ=35)
                                                             tttCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATgcgc                          >  1:553892/1‑100 (MQ=40)
                                                             tttCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATgcgc                          >  1:2820645/1‑100 (MQ=40)
                                                              ttCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATgcgcg                         >  1:1490077/1‑100 (MQ=40)
                                                                 cgcgTTACCAGCAGTGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCt                      >  2:1520579/1‑100 (MQ=255)
                                                                  gcgTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTg                     >  1:1319113/1‑100 (MQ=255)
                                                                      tACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCTACAGTATGTGCATGCGCGCCTgcgc                  >  1:962867/1‑99 (MQ=255)
                                                                      tACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCt                 <  2:1591453/100‑1 (MQ=255)
                                                                        ccAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTg               >  1:1360007/1‑100 (MQ=255)
                                                                         cagcagCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgc              >  2:3171304/1‑100 (MQ=255)
                                                                         cagcagCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTg               <  2:2830827/99‑1 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca             <  1:3226618/100‑1 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca             >  2:1538000/1‑100 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca             >  2:2249541/1‑100 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca             >  2:2761540/1‑100 (MQ=255)
                                                                           gcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcag            <  1:856123/100‑1 (MQ=255)
                                                                           gcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcag            <  1:2837429/100‑1 (MQ=255)
                                                                            cagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcagc           <  2:879954/100‑1 (MQ=255)
                                                                            cagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcagc           <  1:905782/100‑1 (MQ=255)
                                                                               cGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGt        >  2:2329447/1‑100 (MQ=255)
                                                                                   aaCGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCg    <  1:137912/100‑1 (MQ=255)
                                                                                    aCGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCgg   <  2:2274211/100‑1 (MQ=255)
                                                                                    aCGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCgg   <  1:3250093/100‑1 (MQ=255)
                                                                                     cGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGa  <  2:2975346/100‑1 (MQ=255)
                                                                                       |                                                                                                 
CATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGA  >  NC_000913/1634871‑1635055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: