Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,9610AC22.9% 85.5 / 21.4 35I296S (ATC→AGC) stfQQin prophage; putative side tail fibre assembly protein
Reads supporting (aligned to +/- strand):  ref base A (16/11);  new base C (0/8);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 3.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

CATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGACAG  >  NC_000913/1634871‑1635058
                                                                                          |                                                                                                 
cATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgc                                                                                          <  1:1196553/100‑6 (MQ=1)
cATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgc                                                                                          <  1:2568546/100‑6 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                         <  2:2339454/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                         <  2:2339395/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                         <  1:2788028/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                         <  1:2788642/100‑7 (MQ=1)
 aTTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcg                                                                                         <  1:551638/100‑7 (MQ=1)
  ttCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCATCACCGTTAACGCTGCTGgtaacgcgg                                                                                        <  2:2646583/100‑8 (MQ=1)
                atacAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGc                                                                          >  1:1591470/4‑100 (MQ=1)
                                                         gtgtTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCAt                                 >  1:879965/1‑100 (MQ=35)
                                                          tgtTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATg                                >  2:1968450/1‑100 (MQ=35)
                                                             tttCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATgcgc                             >  1:553892/1‑100 (MQ=40)
                                                             tttCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATgcgc                             >  1:2820645/1‑100 (MQ=40)
                                                              ttCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATgcgcg                            >  1:1490077/1‑100 (MQ=40)
                                                                 cgcgTTACCAGCAGTGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCt                         >  2:1520579/1‑100 (MQ=255)
                                                                  gcgTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTg                        >  1:1319113/1‑100 (MQ=255)
                                                                      tACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCTACAGTATGTGCATGCGCGCCTgcgc                     >  1:962867/1‑99 (MQ=255)
                                                                      tACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCt                    <  2:1591453/100‑1 (MQ=255)
                                                                        ccAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTg                  >  1:1360007/1‑100 (MQ=255)
                                                                         cagcagCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgc                 >  2:3171304/1‑100 (MQ=255)
                                                                         cagcagCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTg                  <  2:2830827/99‑1 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca                <  1:3226618/100‑1 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca                >  2:1538000/1‑100 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca                >  2:2249541/1‑100 (MQ=255)
                                                                          agcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgca                >  2:2761540/1‑100 (MQ=255)
                                                                           gcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcag               <  1:856123/100‑1 (MQ=255)
                                                                           gcagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcag               <  1:2837429/100‑1 (MQ=255)
                                                                            cagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcagc              <  2:879954/100‑1 (MQ=255)
                                                                            cagcGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTgcagc              <  1:905782/100‑1 (MQ=255)
                                                                               cGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGt           >  2:2329447/1‑100 (MQ=255)
                                                                                   aaCGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCg       <  1:137912/100‑1 (MQ=255)
                                                                                    aCGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCgg      <  2:2274211/100‑1 (MQ=255)
                                                                                    aCGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCgg      <  1:3250093/100‑1 (MQ=255)
                                                                                     cGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGa     <  2:2975346/100‑1 (MQ=255)
                                                                                        tGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGACAg  >  2:590185/1‑100 (MQ=255)
                                                                                          |                                                                                                 
CATTCTGAATGCCATTATGCAAGCCTCACAATATAGTTAAATGCGATGTTTTTGACGGTGTTTTCCGCGTTACCAGCAGCGTTAACGGTGATGGTGTGTCCATGTGAACCAATCGCAACGGAGTGCGTATGAGCACCAATACCGACAGTATGTGCATGCGCGCCTGCGCTTGCAGCAGTGCCGGACAG  >  NC_000913/1634871‑1635058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: