| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,067,300 | C→T | G8R (GGA→AGA) | insH1 ← | IS5 transposase and trans‑activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,067,300 | 0 | C | T | 95.7% | 134.9 / ‑1.9 | 47 | G8R (GGA→AGA) | insH1 | IS5 transposase and trans‑activator |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (25/20); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCAC > NC_000913/2067222‑2067395 | cgttgcTGGTCTTACTGCTCTTACGGCTGAATTCACTTTCGTCGAAGGTAAGTTGATGACTCATGCTGAACCCTGTTCCATGGCTCAAGATGACCACCAt > 2:598090/6‑100 (MQ=11) tACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGt < 1:826872/100‑1 (MQ=33) gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGa > 2:2748725/1‑100 (MQ=1) cGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAac > 1:998547/1‑100 (MQ=17) ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAaca > 1:2535630/1‑100 (MQ=18) gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAacac > 2:1382013/1‑100 (MQ=25) gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAacac > 1:1789424/1‑100 (MQ=25) cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacc < 1:2744759/100‑1 (MQ=38) cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacc > 1:908431/1‑100 (MQ=25) cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacc > 1:1949425/1‑100 (MQ=25) cGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGAGCTCATAGCAGGGACTTGTTCGCACCTTCCCTAGAAcacc > 2:2291484/1‑100 (MQ=255) gAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcacca < 2:2814487/100‑1 (MQ=255) aaGGTAAGTTGATGGATCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcaccac < 2:2874524/100‑1 (MQ=255) aaGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcaccac < 1:2152882/100‑1 (MQ=255) aaGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAAcaccac > 1:2593863/1‑100 (MQ=255) tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAtt > 1:3179028/1‑100 (MQ=255) tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAtt > 2:40598/1‑100 (MQ=255) tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAtt > 1:2886934/1‑100 (MQ=255) aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAAttt > 2:2241078/1‑100 (MQ=255) aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTc < 2:1468411/100‑1 (MQ=255) aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTc > 2:2820026/1‑100 (MQ=255) ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGc < 1:35242/100‑1 (MQ=255) atgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctct < 1:1536981/100‑1 (MQ=255) gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGctctct < 2:1249350/100‑1 (MQ=255) aCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCtctctc < 1:294227/100‑1 (MQ=255) cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCg > 2:259563/1‑100 (MQ=255) cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCg > 1:2073025/1‑100 (MQ=255) tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCgg < 1:955363/100‑1 (MQ=255) tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCgg > 2:427277/1‑100 (MQ=255) cATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGt > 2:1219292/1‑100 (MQ=255) cATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGt > 2:3039342/1‑100 (MQ=255) atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTa > 1:1214533/1‑100 (MQ=255) atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTa < 1:484475/100‑1 (MQ=255) tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTaa > 2:2551109/1‑100 (MQ=255) gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAg > 1:1676846/1‑100 (MQ=255) gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAg > 2:280738/1‑100 (MQ=255) atgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAgg < 1:402975/100‑1 (MQ=255) aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCAc < 1:790508/100‑1 (MQ=255) aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCAc < 2:2180717/99‑1 (MQ=255) aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACt > 1:362873/1‑100 (MQ=255) aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACt > 2:260893/1‑100 (MQ=255) aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACt < 2:2072999/100‑1 (MQ=255) cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTg < 2:1319956/100‑1 (MQ=255) cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTg < 1:2352093/100‑1 (MQ=255) ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATACCAGGGACTTGTTCGCACCTTCCCTAGAACACCGCAATTTCGCTCTCTCGGTAAGGCACt < 2:490754/98‑1 (MQ=255) cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTg < 2:1303970/98‑1 (MQ=255) gTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTAGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTcac < 2:2054596/100‑1 (MQ=255) | CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGAACACCACAATTTCGCTCTCTCGGTAAGGCACTGTCAC > NC_000913/2067222‑2067395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |