Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,488,027 A→G D237G (GAC→GGC)  lptG → lipopolysaccharide export ABC permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,488,0270AG97.5% 417.4 / ‑2.6 121D237G (GAC→GGC) lptGlipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (60/58);  total (62/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGG  >  NC_000913/4487930‑4488123
                                                                                                 |                                                                                                
cGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCa                                                                                                <  1:2965390/100‑1 (MQ=255)
cGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCa                                                                                                >  1:1133398/1‑100 (MQ=255)
cGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACa                                                                                                >  2:468809/1‑100 (MQ=255)
 gTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCaa                                                                                               >  2:2838184/1‑100 (MQ=255)
 gTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCaa                                                                                               <  1:60647/100‑1 (MQ=255)
 gTCTGTCGCAGGATGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCaa                                                                                               >  1:2142669/1‑100 (MQ=255)
 gTCTGTCGCAGGATGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCaa                                                                                               >  1:2141778/1‑100 (MQ=255)
      tCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTgg                                                                                          <  1:638986/100‑1 (MQ=255)
       cGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTggg                                                                                         >  2:514563/1‑100 (MQ=255)
       cGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTggg                                                                                         >  2:1305313/1‑100 (MQ=255)
       cGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTggg                                                                                         >  1:2097576/1‑100 (MQ=255)
        gCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGc                                                                                        <  2:1170055/100‑1 (MQ=255)
           ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                     >  1:1510557/1‑100 (MQ=255)
           ggTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCgtg                                                                                     >  2:1105428/1‑100 (MQ=255)
               gatgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGtggtgg                                                                                 >  2:2347048/1‑100 (MQ=255)
                atgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgc                                                                                <  1:856083/100‑1 (MQ=255)
                 tgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgcg                                                                               >  2:2597475/1‑100 (MQ=255)
                 tgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgcg                                                                               <  1:2588225/100‑1 (MQ=255)
                 tgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgcg                                                                               >  1:1206147/1‑100 (MQ=255)
                 tgaATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGgcg                                                                               <  1:1614865/100‑1 (MQ=255)
                   aaTCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCt                                                                             <  2:2775775/100‑1 (MQ=255)
                   aaTCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCt                                                                             <  2:369601/100‑1 (MQ=255)
                    atctgatctgACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTg                                                                            <  1:88138/100‑1 (MQ=255)
                    atctgatctgACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTg                                                                            <  2:2911659/100‑1 (MQ=255)
                    atctgatctgACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTg                                                                            >  1:3129313/1‑100 (MQ=255)
                     tctgatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTgg                                                                           <  2:2752981/100‑1 (MQ=255)
                       tgatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGACAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAc                                                                         >  2:2660690/1‑100 (MQ=255)
                       tgatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAc                                                                         <  2:208903/100‑1 (MQ=255)
                       tgatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAc                                                                         >  1:585699/1‑100 (MQ=255)
                        gatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAcc                                                                        >  1:1825451/1‑100 (MQ=255)
                        gatctgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGAcc                                                                        >  2:1190803/1‑100 (MQ=255)
                            tgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGa                                                                    <  1:2710955/100‑1 (MQ=255)
                            tgaCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGa                                                                    <  1:1227136/100‑1 (MQ=255)
                              aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                  <  2:2830996/100‑1 (MQ=255)
                              aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                  <  2:2596010/100‑1 (MQ=255)
                              aCCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATg                                                                  <  2:2768417/100‑1 (MQ=255)
                               ccAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGc                                                                 >  2:2824478/1‑100 (MQ=255)
                                cAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCa                                                                >  1:1227002/1‑100 (MQ=255)
                                 aaTCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCAc                                                               <  1:1329992/100‑1 (MQ=255)
                                 aaTCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCAc                                                               >  2:1965005/1‑100 (MQ=255)
                                 aaTCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCAc                                                               >  1:2802099/1‑100 (MQ=255)
                                   tCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActc                                                             >  1:828550/1‑100 (MQ=255)
                                   tCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActc                                                             >  2:967449/1‑100 (MQ=255)
                                    ccGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActct                                                            >  1:983517/1‑100 (MQ=255)
                                    ccGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActct                                                            >  2:1874189/1‑100 (MQ=255)
                                    ccGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActct                                                            >  2:1943170/1‑100 (MQ=255)
                                     cGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctc                                                           >  2:348110/1‑100 (MQ=255)
                                     cGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctc                                                           >  2:1468590/1‑100 (MQ=255)
                                      gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                          >  1:3026430/1‑100 (MQ=255)
                                      gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                          <  2:2338918/100‑1 (MQ=255)
                                      gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                          <  2:718523/100‑1 (MQ=255)
                                      gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                          >  1:578618/1‑100 (MQ=255)
                                      gAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCActctct                                                          >  1:2051845/1‑100 (MQ=255)
                                         aCAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATc                                                       <  1:3233074/100‑1 (MQ=255)
                                         aCAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATc                                                       <  2:2698408/100‑1 (MQ=255)
                                          cAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCa                                                      <  1:8104/100‑1 (MQ=255)
                                           aGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAg                                                     <  1:1884239/100‑1 (MQ=255)
                                           aGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAg                                                     >  2:1730266/1‑100 (MQ=255)
                                              ttACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCgg                                                  >  1:3058201/1‑100 (MQ=255)
                                                aCCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGtt                                                >  2:622224/1‑100 (MQ=255)
                                                  cGGTTCGCAGACGGTGAGCGGCACCTGTAAAGCCAACCTCACGCCGGGCAAACTTTGCGTGGTGGCGCTTGACCCGGATGCACTCTCTATCAGCGGTTTg                                              <  2:648402/100‑1 (MQ=255)
                                                   ggTTCGCAGACGGTGAGCGGCACCTGGAAAACCAGCCTCACGCCGTGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGc                                             <  2:381208/100‑1 (MQ=255)
                                                   ggTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGc                                             >  2:309381/1‑100 (MQ=255)
                                                     ttCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGcac                                           <  1:967461/100‑1 (MQ=255)
                                                      tCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGcaca                                          <  2:705513/100‑1 (MQ=255)
                                                      tCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGcaca                                          <  1:1577373/100‑1 (MQ=255)
                                                       cGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACaa                                         >  2:2772245/1‑100 (MQ=255)
                                                         cAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACt                                       >  2:2341547/1‑100 (MQ=255)
                                                          aGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACACTCTATCAGCGGTTTGCACAACTa                                      <  2:1437662/100‑1 (MQ=255)
                                                           gACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAt                                     >  1:1360665/1‑100 (MQ=255)
                                                            aCGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTAtg                                    <  2:1226988/100‑1 (MQ=255)
                                                                tGAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGaa                                <  2:871912/100‑1 (MQ=255)
                                                                tGAGCCGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGaa                                <  2:1452737/100‑1 (MQ=255)
                                                                 gAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAg                               <  2:3141532/100‑1 (MQ=255)
                                                                 gAGCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAg                               <  1:762347/100‑1 (MQ=255)
                                                                   gCGGCACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTa                             <  2:2802064/100‑1 (MQ=255)
                                                                       cACCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTg                         >  1:375544/1‑100 (MQ=255)
                                                                        aCCTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGa                        >  2:1056226/1‑100 (MQ=255)
                                                                         ccTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGaa                       >  1:2401991/1‑100 (MQ=255)
                                                                         ccTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGaa                       >  1:3092094/1‑100 (MQ=255)
                                                                          cTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                      <  1:3138333/100‑1 (MQ=255)
                                                                          cTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAg                      <  2:1360651/100‑1 (MQ=255)
                                                                          cTGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCGATCAGCGGTTTGCACAACTATGTGAAGTATCTGaa                       >  2:1801435/1‑99 (MQ=255)
                                                                           tGGAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGt                     >  1:1301372/1‑100 (MQ=255)
                                                                            ggAAAACCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTc                    >  1:3067063/1‑100 (MQ=255)
                                                                               aaaCCAACCTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAg                 <  1:1457131/100‑1 (MQ=255)
                                                                                 accaaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCg               >  2:2026946/1‑100 (MQ=255)
                                                                                  ccaaccCCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGTACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCgg              <  2:2725395/100‑1 (MQ=255)
                                                                                    aaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTc            <  1:1831219/100‑1 (MQ=255)
                                                                                    aaccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTc            <  1:500914/100‑1 (MQ=255)
                                                                                      ccTCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAg          <  1:2597508/100‑1 (MQ=255)
                                                                                        tCACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGa        <  2:169141/100‑1 (MQ=255)
                                                                                         cACGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGAt       >  2:1344593/1‑100 (MQ=255)
                                                                                           cGCCGGGCAAACTGGGCGTGGTGGCGCTGGCCCCGGATGCACTCTCTCTCAGCGGTTTGCACCACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGc     <  2:1901609/100‑1 (MQ=255)
                                                                                           cGCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGc     >  2:166547/1‑100 (MQ=255)
                                                                                            gCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGcc    <  2:2097550/100‑1 (MQ=255)
                                                                                            gCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGcc    <  2:1423210/100‑1 (MQ=255)
                                                                                            gCCGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGcc    <  1:720172/100‑1 (MQ=255)
                                                                                             ccGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCg   <  2:585690/100‑1 (MQ=255)
                                                                                             ccGGGCAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCg   >  1:1880218/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGTCTGTCGCAGGTTGATGAATCTGATCTGACCAATCCGAAACAGATTACCGGTTCGCAGACGGTGAGCGGCACCTGGAAAACCAACCTCACGCCGGACAAACTGGGCGTGGTGGCGCTGGACCCGGATGCACTCTCTATCAGCGGTTTGCACAACTATGTGAAGTATCTGAAGTCGAGCGGTCAGGATGCCGG  >  NC_000913/4487930‑4488123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: