BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,802	A→G	V222A (GTA→GCA)	focA ←	formate channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,802	0	A	G	99.2%	415.3 / ‑4.7	117	V222A (GTA→GCA)	focA	formate channel
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (50/66); total (50/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 117 total aligned reads displayed.

TTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953706‑953897

 tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                             <  1:2390837/100‑1 (MQ=255)

 tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                             <  2:588948/100‑1 (MQ=255)

 tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                             >  2:2021089/1‑100 (MQ=255)

 tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                             <  2:588961/100‑1 (MQ=255)

  gaccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATa                                                                                            >  1:1590630/5‑100 (MQ=255)

    ccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  2:2960801/3‑100 (MQ=255)

    ccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  2:2355013/3‑100 (MQ=255)

    ccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  1:1551280/3‑100 (MQ=255)

    ccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  1:2737336/3‑100 (MQ=255)

    cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          >  2:936952/1‑100 (MQ=255)

    cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                          <  2:2070670/100‑1 (MQ=255)

    cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                           >  2:2614516/1‑99 (MQ=255)

     aGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAccc                                                                                         >  2:2415537/1‑100 (MQ=255)

      gTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCa                                                                                        <  1:1334125/100‑1 (MQ=255)

        gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  2:1436582/1‑100 (MQ=255)

        gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      <  2:1312056/100‑1 (MQ=255)

          tgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCgg                                                                                    >  2:282742/1‑100 (MQ=255)

           gaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCggg                                                                                   >  1:2617963/1‑100 (MQ=255)

           gaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCggg                                                                                   <  2:1246250/100‑1 (MQ=255)

             aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                 >  1:2222632/1‑100 (MQ=255)

             aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                 >  1:1644807/1‑100 (MQ=255)

              aTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATc                                                                                <  2:2900097/100‑1 (MQ=255)

                tcatcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCAt                                                                              <  2:3058791/100‑1 (MQ=255)

                tcatcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCAt                                                                              <  1:3264822/100‑1 (MQ=255)

                tcatcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCAt                                                                              <  2:764810/100‑1 (MQ=255)

                tcatcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCAt                                                                              <  1:2392330/100‑1 (MQ=255)

                tcatcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCAt                                                                              <  2:2308469/100‑1 (MQ=255)

                 catcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATa                                                                             >  1:2855493/1‑100 (MQ=255)

                   tcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaaa                                                                           <  1:2056040/100‑1 (MQ=255)

                   tcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaaa                                                                           >  1:2987203/1‑100 (MQ=255)

                      cGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACAt                                                                        <  2:2679962/100‑1 (MQ=255)

                       ggTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTAGCGGATTGCAATACCCATCGGGATCATAAACATg                                                                       >  2:1726228/1‑100 (MQ=255)

                         tCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGtt                                                                     <  1:2040040/100‑1 (MQ=255)

                         tCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGtt                                                                     <  2:1933805/100‑1 (MQ=255)

                           aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                   <  2:2617930/100‑1 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  1:1299407/1‑100 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  2:712682/1‑100 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  2:1588845/100‑1 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  1:36299/1‑100 (MQ=255)

                            gctGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  2:1974654/98‑1 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             <  1:1770989/100‑1 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             >  2:2607364/1‑100 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAACTCCGGGGATGCGAAGTCGCTGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             <  2:1781682/100‑1 (MQ=255)

                                   aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                           <  2:36298/100‑1 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        <  1:1843745/100‑1 (MQ=255)

                                      aTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGc                                                        >  1:2257218/1‑100 (MQ=255)

                                         ttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                     <  1:1223609/100‑1 (MQ=255)

                                            cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaaa                                                  >  1:2323876/1‑100 (MQ=255)

                                            cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaaa                                                  >  1:1833640/1‑100 (MQ=255)

                                            cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaa                                                    >  1:2106651/1‑98 (MQ=255)

                                              gTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAcc                                                <  2:44961/100‑1 (MQ=255)

                                              gTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAcc                                                >  1:771592/1‑100 (MQ=255)

                                                gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              <  1:2575783/100‑1 (MQ=255)

                                                 cAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCt                                             <  1:58601/100‑1 (MQ=255)

                                                 cAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCt                                             >  2:2953385/1‑100 (MQ=255)

                                                  aGAACCGACTGCGGTCCAAAATTCCGGGGATGTGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                            <  2:1071832/100‑1 (MQ=255)

                                                  aGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                            <  2:2512306/100‑1 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                           >  2:198166/1‑100 (MQ=255)

                                                    aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                          <  1:841786/100‑1 (MQ=255)

                                                     aCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGca                                         >  2:2355727/1‑100 (MQ=255)

                                                        gACTGCGGTCCAAAATTCCGGGGATGTGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      >  1:1857405/1‑100 (MQ=255)

                                                        gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      >  2:45095/1‑100 (MQ=255)

                                                        gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      >  2:1742675/1‑100 (MQ=255)

                                                         aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaacaa                                     <  1:2415564/100‑1 (MQ=255)

                                                           tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                   <  1:257002/100‑1 (MQ=255)

                                                           tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                   <  1:2614549/100‑1 (MQ=255)

                                                            gCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaaca                                  >  1:541773/1‑100 (MQ=255)

                                                                tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                              >  1:1736223/1‑100 (MQ=255)

                                                                tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCACAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                              >  1:2180559/1‑100 (MQ=255)

                                                                 ccAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATTGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgcg                             >  1:542560/1‑100 (MQ=255)

                                                                 ccAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgcg                             <  1:1967903/100‑1 (MQ=255)

                                                                  cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAGTACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                            >  2:1963007/1‑100 (MQ=255)

                                                                  cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                            >  1:1181688/1‑100 (MQ=255)

                                                                    aaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAcc                          <  1:2960836/100‑1 (MQ=255)

                                                                    aaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAcc                          <  2:2257192/100‑1 (MQ=255)

                                                                      aTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCgg                        <  2:2943491/100‑1 (MQ=255)

                                                                       ttCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                       <  2:1181674/100‑1 (MQ=255)

                                                                        tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                      <  2:2737302/100‑1 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                    <  1:2424202/100‑1 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                    <  2:1331123/100‑1 (MQ=255)

                                                                              gATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCa                <  1:2281017/100‑1 (MQ=255)

                                                                               aTGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCAt               >  2:1074764/1‑100 (MQ=255)

                                                                               aTGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCAt               <  1:208766/100‑1 (MQ=255)

                                                                                  cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt            >  1:638571/1‑100 (MQ=255)

                                                                                  cGAAGTCGCGGATTGCAATACAAATCGGGATCATAAACATGTTTGCGATACTGTGATCAAAACCGCTGGCAACAAACATAGCGACCGGCAGCACCATAAt            >  2:331154/1‑100 (MQ=255)

                                                                                   gAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATg           <  2:1551264/100‑1 (MQ=255)

                                                                                   gAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATg           <  1:1737531/100‑1 (MQ=255)

                                                                                    aaGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGa          <  1:936964/100‑1 (MQ=255)

                                                                                    aaGTCGCGGATCGAACTACCTATCAGGATCATACACACCTTCGCTATACTGGACTAAAACCCGCAGGCAACAAACATCGCGACCGGCAGCACCATAAta           <  2:2397460/99‑2 (MQ=255)

                                                                                      gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        <  1:2028858/100‑1 (MQ=255)

                                                                                      gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        >  1:1889137/1‑100 (MQ=255)

                                                                                      gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        >  1:3219080/1‑100 (MQ=255)

                                                                                        cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      <  1:2021114/100‑1 (MQ=255)

                                                                                         gcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCt     >  2:3160597/1‑100 (MQ=255)

                                                                                          cgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCtt    <  1:2355040/100‑1 (MQ=255)

                                                                                          cgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCtt    <  1:117651/100‑1 (MQ=255)

                                                                                           ggATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCttt   >  1:1866957/1‑100 (MQ=255)

                                                                                            gATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTg  <  2:394680/100‑1 (MQ=255)

                                                                                            gATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTg  <  1:1436596/100‑1 (MQ=255)

                                                                                            gATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCttta  <  1:428710/100‑2 (MQ=255)

TTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953706‑953897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`