Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,469,733 (T)8→7 100% coding (603/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,7260T.71.2% 238.8 / 188.7 139coding (596/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (18/22);  new base . (47/52);  total (65/74)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 168 total aligned reads displayed.

CAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTG  >  NC_000913/2469628‑2469824
                                                                                                  |                                                                                                  
cAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatatt                                                                                                   >  1:1374339/1‑100 (MQ=255)
  aTAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttt                                                                                                 >  1:552046/1‑100 (MQ=255)
    aaaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt                                                                                               <  1:1643854/100‑1 (MQ=255)
     taTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                              <  1:1628283/99‑1 (MQ=255)
     aaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                              <  1:1220360/100‑1 (MQ=255)
      aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATGTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttta                                                                                             >  2:1628267/1‑99 (MQ=255)
      aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt                                                                                             <  2:1349836/100‑1 (MQ=255)
      aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttg                                                                                             <  1:2472941/100‑2 (MQ=255)
      aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttg                                                                                             <  1:2417689/100‑2 (MQ=255)
      aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttg                                                                                             <  2:1512747/100‑2 (MQ=255)
       tCTATGCTTTTGCTATTTTTTTCTTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg                                                                                            <  2:1298704/100‑1 (MQ=255)
       tCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttg                                                                                             <  1:476836/99‑2 (MQ=255)
        cTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgat                                                                                           <  1:789324/100‑4 (MQ=255)
         tATGCTTTTGCTATTTTTTTCGTTGTTAATAATATCTACTGCTAAAAATAAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgata                                                                                          <  1:921203/100‑5 (MQ=255)
         tATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATATTGTATTCATATTATAtttttttgat                                                                                           <  2:1186665/99‑4 (MQ=255)
         tATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgata                                                                                          >  1:320837/1‑96 (MQ=255)
         tATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgata                                                                                          <  1:2556612/100‑5 (MQ=255)
         tATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGAt                                                                                          <  2:736927/100‑1 (MQ=255)
          aTGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgatan                                                                                         <  1:1690867/100‑6 (MQ=255)
          aTGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATATTTTTGATAg                                                                                        >  2:1745346/1‑100 (MQ=255)
           tGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAg                                                                                        <  2:2339435/100‑1 (MQ=255)
            gCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGac                                                                                      >  2:1664582/1‑100 (MQ=255)
              ttttGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGaca                                                                                     <  2:408787/100‑1 (MQ=255)
                  gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGaaa                                                                                     >  2:1581768/1‑94 (MQ=255)
                  gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                                <  1:1565385/100‑1 (MQ=255)
                   cTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAAc                                                                                <  1:666230/100‑1 (MQ=255)
                      tttttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAat                                                                            <  1:336304/100‑1 (MQ=255)
                      tttttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAat                                                                            <  1:2206752/100‑1 (MQ=255)
                       ttttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAatt                                                                           <  1:2248335/100‑1 (MQ=255)
                       ttttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAatt                                                                           >  1:83625/1‑100 (MQ=255)
                       ttttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAatt                                                                           <  2:1274521/100‑1 (MQ=255)
                         ttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAattat                                                                         <  1:2352810/100‑1 (MQ=255)
                           ttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAAttatta                                                                       <  2:195052/100‑1 (MQ=255)
                           ttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAAttatta                                                                       <  2:185683/100‑1 (MQ=255)
                             cGTTACTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                     <  1:2349400/100‑1 (MQ=255)
                              gTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAAt                                                                    >  1:1187724/1‑100 (MQ=255)
                                tGCTAATAATATCTACTGCTAAAAATAAATTTATATTAACCTCACTAATAGTGTATTCATATTATATTTTTTTTGATATACACAAAATAGATATTAAATc                                                                   >  2:714665/1‑100 (MQ=255)
                                  cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTATAGACATAAACTAATTATTAAATCTGt                                                                >  2:877568/1‑100 (MQ=255)
                                  cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTAGATAGACACAAATAAATTATTaa                                                                      >  1:1176760/1‑94 (MQ=255)
                                   taataaTATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                                <  2:2190008/100‑1 (MQ=255)
                                   taataaTATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTa                                                               >  2:2048414/1‑100 (MQ=255)
                                    aataatATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTa                                                               >  2:2286043/1‑100 (MQ=255)
                                       aataTCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAAGTAATTATTAAATCTGTAata                                                            <  2:1614578/100‑1 (MQ=255)
                                        atatCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATg                                                          <  1:2048436/100‑1 (MQ=255)
                                        atatCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATg                                                          <  1:1373569/100‑1 (MQ=255)
                                            ntACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGAAACAAACTAATTATTAAATCTGTAATATGCgt                                                       >  2:312672/2‑99 (MQ=255)
                                            cTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtg                                                      <  2:92054/100‑1 (MQ=255)
                                            cTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtg                                                      <  1:1498991/100‑1 (MQ=255)
                                               cTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtggtg                                                   >  1:2582680/1‑100 (MQ=255)
                                               cTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAATAATTATTAAATCTGTAATATGCgtggtg                                                   >  1:2151926/1‑100 (MQ=255)
                                               cTGCTAAAAATCAATTTATATTAACCCCACTAAAAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtggtg                                                   >  2:1752005/1‑100 (MQ=255)
                                                 gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATATGTAATATGAGTGatgt                                                  >  1:1144482/1‑100 (MQ=255)
                                                 gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGtgtg                                                 >  2:2029256/1‑100 (MQ=255)
                                                 gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGtgtg                                                 >  2:1905099/1‑100 (MQ=255)
                                                 gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGtgtg                                                 <  2:5602/100‑1 (MQ=255)
                                                   tAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgct                                               >  2:769815/1‑100 (MQ=255)
                                                    aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgct                                               <  2:608298/100‑1 (MQ=255)
                                                    aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTTTTTTTTTTTACTAATTATTAAATCTGTAATATGCGTCGTGtgctt                                              >  2:1990575/1‑100 (MQ=255)
                                                    aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgctt                                              >  1:1385096/1‑100 (MQ=255)
                                                     aaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgctt                                              <  2:607592/100‑1 (MQ=255)
                                                       aaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTTTTATTTACAAACTAATTATTAAATCTGTAATATGCGTGGTGttcttgc                                            >  2:340754/1‑100 (MQ=255)
                                                        aTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgcttgct                                           <  2:194056/100‑1 (MQ=255)
                                                         tCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGcttgcttg                                         >  2:688102/1‑100 (MQ=255)
                                                         tCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGcttgcttg                                         <  1:1422625/100‑1 (MQ=255)
                                                              ttATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCa                                    <  2:320835/100‑1 (MQ=255)
                                                               tataTTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCaa                                   >  2:2134950/1‑100 (MQ=255)
                                                                atatTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAat                                  >  1:1342630/1‑100 (MQ=255)
                                                                  atTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTTTAATATGCGTGGTGTGCTTGCTTGCTTCCatat                                >  2:2446772/1‑100 (MQ=255)
                                                                    tAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATAttt                              >  2:2319343/1‑100 (MQ=255)
                                                                     aaCCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATAttt                              <  1:1763158/100‑1 (MQ=255)
                                                                     aaCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTg                             <  2:2496552/100‑1 (MQ=255)
                                                                      aCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGc                            <  1:43873/100‑1 (MQ=255)
                                                                       ccccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                           <  1:1664598/100‑1 (MQ=255)
                                                                       ccccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                           <  2:2488719/100‑1 (MQ=255)
                                                                        cccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGATTTGCTTGCTTGCGTCAATATTTGCaa                          >  1:442517/1‑100 (MQ=255)
                                                                         ccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAat                         >  1:1069245/1‑100 (MQ=255)
                                                                          cACTAATAGTGTATTCATATTATATTTTTTTTTTTTTTTTAAACTAATTATAAAATCTGTAATATGCGTGGTGTGCTTGATTGCGTCAATAttttc                            >  2:639333/1‑94 (MQ=255)
                                                                           aCTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAatat                       <  1:1929221/100‑1 (MQ=255)
                                                                           aCTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAata                        <  1:1810285/99‑1 (MQ=255)
                                                                             tAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCt                     >  2:2108889/1‑100 (MQ=255)
                                                                             tAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCt                     >  2:2040960/1‑100 (MQ=255)
                                                                             tAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCt                     <  2:552042/100‑1 (MQ=255)
                                                                              aaTAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCt                     <  1:1894754/100‑1 (MQ=255)
                                                                              aaTAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCtt                    >  1:1605934/1‑100 (MQ=255)
                                                                              aaTAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCtt                    >  2:1057775/1‑100 (MQ=255)
                                                                              aaTAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCtt                    >  1:1742319/1‑100 (MQ=255)
                                                                                tAGTTTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTa                   <  1:2407056/100‑1 (MQ=255)
                                                                                tAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTa                   <  1:627120/100‑1 (MQ=255)
                                                                                 aGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAtt                 >  2:277080/1‑100 (MQ=255)
                                                                                  gtgtATTCATATTTTATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTc                >  1:1782359/1‑100 (MQ=255)
                                                                                  gtgtATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAtt                 >  2:2334232/1‑100 (MQ=255)
                                                                                  gtgtATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGATTGCGTCAATATTTGCAATATCTTATTc                >  1:1307270/1‑100 (MQ=255)
                                                                                   tttATTCATATTATATTTTTTTGATATACACAAATTAATTATTAAATCTGTAATATGCGGGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTc                <  1:108142/97‑1 (MQ=255)
                                                                                    gtATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCa               <  2:150450/100‑1 (MQ=255)
                                                                                       ttCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCaaaa            <  2:1764090/100‑1 (MQ=255)
                                                                                         cATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAgg          >  1:2325693/1‑99 (MQ=255)
                                                                                          atattatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGgtg        <  2:1292345/100‑1 (MQ=255)
                                                                                          atattatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGgt         >  2:120841/1‑99 (MQ=255)
                                                                                                tattTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTgttgtt   <  2:726578/100‑1 (MQ=255)
                                                                                                 attTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGttgttg  <  1:1292433/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTGTTG  >  NC_000913/2469628‑2469824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: