Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,183,350 Δ1 bp 19.7% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3500A.19.7% 321.4 / 80.1 117intergenic (+27/+463)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (57/37);  new base . (12/11);  total (69/48)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01

Only 100 of 118 total aligned reads displayed.

TATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGC  >  NC_000913/3183252‑3183449
                                                                                                   |                                                                                                   
tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACCGCGGGAATTTGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                    <  2:185346/100‑1 (MQ=255)
   ggCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGa                                                                                                 >  2:424724/1‑100 (MQ=255)
   ggCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGa                                                                                                 >  1:1142816/1‑100 (MQ=255)
       tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAAc                                                                                             <  2:1384627/100‑1 (MQ=255)
        aCTATGTGGAATGTCAGTGATGTTATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACt                                                                                            <  1:323559/100‑1 (MQ=38)
         cTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACtt                                                                                           >  1:2175091/1‑100 (MQ=255)
          tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCATAAGTTGTCCATGCGCTGGACAACTTc                                                                                          <  2:1281038/100‑1 (MQ=39)
          tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTc                                                                                          <  1:1127405/100‑1 (MQ=39)
          tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACtt                                                                                           <  2:851308/99‑1 (MQ=255)
          tATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTc                                                                                          <  1:811823/100‑1 (MQ=255)
           ttgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCt                                                                                         <  2:1087141/99‑1 (MQ=38)
           aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGGAGTTGTCCAGCGCATGGACAACTTCt                                                                                         >  1:1977985/1‑100 (MQ=255)
           aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCt                                                                                         <  1:723240/100‑1 (MQ=38)
           aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCt                                                                                         >  1:1880777/1‑100 (MQ=255)
            tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCta                                                                                        >  1:1087151/1‑99 (MQ=38)
            tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTg                                                                                        >  1:1232746/1‑100 (MQ=255)
             gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                       >  1:2159382/1‑100 (MQ=255)
             gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                       >  1:2520786/1‑100 (MQ=255)
              tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgat                                                                                      <  2:1147597/100‑3 (MQ=38)
              tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgat                                                                                      <  1:1747293/100‑3 (MQ=38)
              tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGcc                                                                                      >  1:720590/1‑100 (MQ=255)
               ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                     >  1:2121249/1‑100 (MQ=255)
               ggAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                     <  2:1306802/100‑1 (MQ=255)
                gAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCa                                                                                     <  2:1819511/99‑1 (MQ=255)
                  aTGTTAGTGATTGGATTCAGTTTAGTGCTGCTACAAACGGCTGGAATTGGTTGAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCt                                                                                  <  1:2183702/100‑1 (MQ=255)
                     tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgttgttt                                                                               <  1:356108/100‑10 (MQ=25)
                      cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                              >  2:597106/1‑100 (MQ=255)
                       aGTGATGGGATTCAGTTTAGTGCTGTTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACg                                                                             <  1:464012/100‑1 (MQ=255)
                        gTGATGGGGTTCAGTTTAGTGCTGATACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAATTGTCAAGCGCATGGACAACtcatacca                                                                                     >  2:2544106/1‑84 (MQ=255)
                          gATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaaa                                                                          >  2:483398/1‑100 (MQ=255)
                          gATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaaa                                                                          >  1:247232/1‑100 (MQ=255)
                             gggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGcc                                                                       >  2:2344158/1‑100 (MQ=255)
                              ggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCt                                                                      >  1:1547235/1‑100 (MQ=255)
                                   cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                                 >  1:175566/1‑100 (MQ=255)
                                     gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTATACCAGCTTCTCGAAAGCCTTATTCTa                                                               >  2:106704/1‑100 (MQ=255)
                                      tttAGTGCTGCTACAATCGTCGTTAATTGGTTAAAAACAATATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAc                                                              <  1:2344188/100‑1 (MQ=255)
                                         aGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGTAGTTGTCCAACGCATGGACAACTTCTGCCAGCTACACAAAAGCCGGATTCGACCtt                                                           >  1:2386180/1‑100 (MQ=255)
                                            gctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGATTCACGAAAGCCTTATTCTACCTTTTc                                                        >  1:2265365/1‑100 (MQ=255)
                                             ctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                       <  1:1030331/100‑1 (MQ=255)
                                             ctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                       <  1:1164233/100‑1 (MQ=255)
                                               gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                     <  2:1166988/100‑1 (MQ=255)
                                               gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                     >  2:1683193/1‑100 (MQ=255)
                                                 tACAAACGGCGGGAATTGGTTAAAAACAACATCAGGAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                   <  2:1977967/100‑1 (MQ=255)
                                                 tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                   >  1:1206690/1‑100 (MQ=255)
                                                 tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                   <  2:1587252/100‑1 (MQ=255)
                                                   cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAACCTTATTCTACCTTTTCGGCTGtc                                                 >  1:1193241/1‑100 (MQ=255)
                                                     aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAAAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctc                                               >  2:2342196/1‑100 (MQ=255)
                                                      actgcgtGAATTTGTTAAAAACAACATCAGAAGTTTTCCAGCGCATTGACAACTTCTGCCAGCTTCACAAAAGCCTTATTCTACCTTTTCGGCTGtctc                                               <  2:549224/96‑1 (MQ=255)
                                                        ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCTAGCTTCACGAAAGCATTATTCTACATTTTCGCCTGtctct                                              >  1:1543133/1‑98 (MQ=255)
                                                        ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                            >  2:1930420/1‑100 (MQ=255)
                                                          cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGATAACTTCTGCCAGCTTCTCTAAAGCCTTATTCTTCTTTTTCGGCTGtctgttctc                                          <  2:1264957/100‑1 (MQ=255)
                                                          cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                          >  1:2146255/1‑100 (MQ=255)
                                                          cggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                          >  2:1118022/1‑100 (MQ=255)
                                                           gggAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctct                                         <  1:597110/100‑1 (MQ=255)
                                                            ggAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTtctctc                                        >  2:535165/1‑100 (MQ=255)
                                                            ggAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTtctctc                                        >  1:1380435/1‑100 (MQ=255)
                                                             gAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCg                                       <  1:1809943/100‑1 (MQ=255)
                                                                ttGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTAc                                    >  2:2092495/1‑100 (MQ=255)
                                                                ttGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTAc                                    >  1:1626950/1‑100 (MQ=255)
                                                                  ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                  >  2:398075/1‑100 (MQ=255)
                                                                  ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                  >  2:957329/1‑100 (MQ=255)
                                                                  ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                                  >  1:2295044/1‑100 (MQ=255)
                                                                  ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTACTGCCCGATTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACt                                   >  2:2366101/1‑99 (MQ=255)
                                                                   gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                 >  2:975487/1‑100 (MQ=255)
                                                                   gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                                 >  1:1910750/1‑100 (MQ=255)
                                                                    ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                                >  1:2476665/1‑100 (MQ=255)
                                                                    ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                                >  2:600603/1‑100 (MQ=255)
                                                                       gatctgctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                             <  2:789995/90‑1 (MQ=25)
                                                                       gatctgctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                             <  2:409550/90‑1 (MQ=25)
                                                                       aaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                             <  1:1850073/100‑1 (MQ=255)
                                                                       aaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                             >  1:2305006/1‑100 (MQ=255)
                                                                        aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                            <  2:4286/100‑1 (MQ=255)
                                                                         gaagctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGt                           >  1:240369/9‑100 (MQ=25)
                                                                           caacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTATACCTTTTCGGCTGTATATTCTCTCGTACTGTTTAAGTAt                         >  1:539108/1‑100 (MQ=255)
                                                                            gctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTTACGAAAGCCTTATTCTACCTTTTCGTCTGTCTCTTCTCTCGTACTGTTTaaagatc                        >  1:790003/6‑95 (MQ=25)
                                                                            gctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaaagatc                        >  1:409553/6‑95 (MQ=25)
                                                                             ctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttt                       >  2:1135713/5‑100 (MQ=37)
                                                                              tggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTg                      >  1:842975/4‑100 (MQ=38)
                                                                              caTCAGAAGTTTTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttt                       <  2:1193860/99‑1 (MQ=255)
                                                                               aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                     >  1:1708609/1‑100 (MQ=255)
                                                                               aTCAGAAGTTGTCAAGCGCATGGACAACTTCTGCCAGCGTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                     >  2:1637349/1‑100 (MQ=255)
                                                                                tCAGAAGTTGTCCAGTGCATGGACAACTTCTGCTAGCTTCCCGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                     <  1:1550985/99‑1 (MQ=255)
                                                                                tCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGtt                    >  2:1021775/1‑100 (MQ=255)
                                                                                 cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc                   >  1:2494488/1‑100 (MQ=255)
                                                                                 cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc                   >  1:2092691/1‑100 (MQ=255)
                                                                                  aGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                  <  2:247230/100‑1 (MQ=255)
                                                                                   gAAGTTGTCCAGCGCATGGACCACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                  <  2:1444416/99‑1 (MQ=255)
                                                                                   gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                 <  2:53818/100‑1 (MQ=255)
                                                                                     aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg               >  2:302785/1‑100 (MQ=39)
                                                                                     aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg               >  1:1927412/1‑100 (MQ=39)
                                                                                     aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg               >  1:1741722/1‑100 (MQ=39)
                                                                                       ttGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCt             <  1:1227433/100‑1 (MQ=255)
                                                                                        tGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCtt            >  1:166336/1‑100 (MQ=255)
                                                                                         gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc           >  2:240969/1‑100 (MQ=255)
                                                                                         gTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTc           >  1:1422887/1‑100 (MQ=255)
                                                                                          tCCAGCGCATTGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTGCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCa          <  1:106705/100‑1 (MQ=255)
                                                                                               gcgcATGGACATCTTCTGCCAGCTTCACTAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc       <  2:810117/99‑1 (MQ=255)
                                                                                               gcgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTATCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCa      >  1:2311256/1‑100 (MQ=255)
                                                                                                  cATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTg   <  1:2220723/100‑1 (MQ=255)
                                                                                                   aTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGc  >  2:154902/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGC  >  NC_000913/3183252‑3183449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: