Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,512,561 Δ1 bp 64.3% intergenic (‑217/+73) yhfZ ← / ← trpS putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,512,5600T.64.3% 107.8 / 193.7 112intergenic (‑216/+74)yhfZ/trpSputative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (21/19);  new base . (32/40);  total (53/59)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAA  >  NC_000913/3512461‑3512656
                                                                                                   |                                                                                                
aTAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAt                                                                                                  >  1:897402/1‑100 (MQ=255)
 tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATTCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt                                                                                                 >  2:579592/1‑100 (MQ=255)
 tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtt                                                                                                 >  1:1225954/1‑100 (MQ=255)
 tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAta                                                                                                 >  2:470455/1‑99 (MQ=255)
  aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtac                                                                                                >  2:1170751/1‑98 (MQ=255)
  aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTa                                                                                                >  2:1888877/1‑100 (MQ=255)
   aaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtact                                                                                               >  2:774477/1‑97 (MQ=255)
       aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACTCTTATCAGTCCTACATTTCCTTGCAATATGTGCATACTTTGt                                                                                          <  2:471913/100‑1 (MQ=38)
       aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTg                                                                                           <  2:2420712/100‑1 (MQ=255)
       aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTg                                                                                           <  2:2118951/100‑1 (MQ=255)
        aCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTa                                                                                         <  2:897393/100‑1 (MQ=255)
         ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAg                                                                                        <  1:1033442/100‑1 (MQ=255)
         ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAg                                                                                        >  1:1996079/1‑100 (MQ=255)
          cGGGTTTCCCCGGTTTCAGAGTTATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAgg                                                                                       <  1:573656/100‑1 (MQ=255)
          cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAgg                                                                                       <  1:2350991/100‑1 (MQ=255)
          cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAgg                                                                                       <  2:1662739/100‑1 (MQ=255)
          cGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAgg                                                                                       <  2:2117369/100‑1 (MQ=255)
             gTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCg                                                                                    <  1:1136638/100‑1 (MQ=255)
              tttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCgg                                                                                   <  2:2197168/100‑1 (MQ=255)
              tttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCgg                                                                                   <  1:279846/100‑1 (MQ=255)
               ttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCgg                                                                                   >  1:1551944/1‑100 (MQ=255)
               ttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCgg                                                                                   <  2:1038604/100‑1 (MQ=255)
               ttCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGACCgg                                                                                   >  1:909944/1‑100 (MQ=255)
                tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGAt                                                                                 <  2:1994207/100‑1 (MQ=255)
                tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGAt                                                                                 >  2:219715/1‑100 (MQ=255)
                tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTACAATATGTGCATACTTTGTAGGCCGGa                                                                                  >  1:1308994/1‑99 (MQ=255)
                 ccccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGAt                                                                                 >  1:2131109/1‑100 (MQ=255)
                  cccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATAGTTTGTAGGCCGGATaa                                                                               >  2:1552512/1‑100 (MQ=255)
                     ggTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGc                                                                            <  1:2620785/100‑1 (MQ=255)
                     ggTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTACAATATGTGCATTACTTTGTAGGCCGGATAAg                                                                              >  1:1668617/1‑99 (MQ=255)
                         tCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTc                                                                        >  2:1816748/1‑100 (MQ=255)
                          cAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTc                                                                        <  2:2443448/100‑1 (MQ=255)
                          cAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCa                                                                       <  1:1955840/100‑1 (MQ=255)
                             agTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgc                                                                    <  1:2366965/100‑1 (MQ=255)
                             agTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgc                                                                    <  1:2451449/100‑1 (MQ=255)
                              gTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                   <  2:2057711/100‑1 (MQ=255)
                               tgatgaTAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                   <  2:1225942/100‑1 (MQ=255)
                               tgatgaTAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                  <  1:762468/100‑1 (MQ=255)
                               tgatgaTAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                  <  1:526968/100‑1 (MQ=255)
                                 atgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                 >  2:1366082/1‑100 (MQ=255)
                                 atgatAAAAGCAAAATTGCCTGATGAGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                 >  2:1158525/1‑100 (MQ=255)
                                  tgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                >  2:1259350/1‑100 (MQ=255)
                                     tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTTCAATATGTGCATAATTTATAGGCCGGATAAGGCGTTCACTCCGCATcc                                                             >  1:58892/1‑99 (MQ=25)
                                     tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                            >  1:459742/1‑100 (MQ=255)
                                     tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCATTCACGCCGCATCCg                                                            >  1:2359583/1‑100 (MQ=255)
                                      aaaaGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                           >  1:589838/1‑100 (MQ=255)
                                      aaaaGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                           >  1:494842/1‑100 (MQ=255)
                                      aaaaGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATATGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                             >  1:494843/1‑98 (MQ=255)
                                        aaGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                         >  2:727389/1‑100 (MQ=255)
                                          gCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                        >  1:2218565/1‑100 (MQ=255)
                                           cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTCCAATATGTGCATACTTTGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCAtaa                                                      >  1:1151697/1‑98 (MQ=37)
                                            aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                                                     <  1:1761672/100‑1 (MQ=255)
                                               aTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACa                                                   >  1:801804/1‑100 (MQ=255)
                                                ttGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa                                                  <  1:1986524/100‑1 (MQ=255)
                                                  gCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCATGAACAAAg                                                <  2:2403113/100‑1 (MQ=255)
                                                  gCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg                                                <  2:2403363/100‑1 (MQ=255)
                                                  gCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAgc                                               >  1:2178456/1‑100 (MQ=255)
                                                    cTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAgcg                                              >  1:2052181/1‑100 (MQ=255)
                                                    cTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAgcg                                              >  2:1277910/1‑100 (MQ=255)
                                                     tGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCATGAACAAAgcgc                                             >  1:2618558/1‑100 (MQ=255)
                                                       aTGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGTATACTTTGTAGGCCGGATAAGGCGATCACGCCGCATCCGGCATGAACAAAGCGCaa                                           <  1:876190/99‑1 (MQ=25)
                                                        tGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGCTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAAtt                                         >  1:1969500/1‑100 (MQ=255)
                                                        tGCGCTACGCTTATCAGGCCTACATTACCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACACCGCATCCGGCATGAACAAAGCGCAAt                                          >  1:1610956/1‑100 (MQ=255)
                                                          cgcTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTg                                       <  1:462746/100‑1 (MQ=255)
                                                           gcTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAAttt                                        >  2:2399828/1‑99 (MQ=255)
                                                            cTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc                                     <  1:676115/100‑1 (MQ=255)
                                                             tACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc                                     <  2:1125780/100‑1 (MQ=255)
                                                              aCGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg                                   >  1:1878101/1‑100 (MQ=255)
                                                               cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg                                   <  1:2539867/100‑1 (MQ=255)
                                                               cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGc                                  <  2:26731/100‑1 (MQ=255)
                                                                   tATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAAt                               <  2:1668601/100‑1 (MQ=255)
                                                                   tATCAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCATTTTGCCAGCAATa                              <  1:1800283/100‑1 (MQ=255)
                                                                     tCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAg                             >  2:439863/1‑100 (MQ=255)
                                                                      cAGGCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTg                           <  2:1155660/100‑1 (MQ=255)
                                                                        ggCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGaa                         >  2:1425487/1‑100 (MQ=255)
                                                                        ggCCTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGaa                         <  1:219717/100‑1 (MQ=255)
                                                                          ccTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAAt                        >  1:1155136/1‑100 (MQ=255)
                                                                          ccTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCAGATAAGGCGTTCACTCCGCATCCGGCATGATCAAAGCGCAATTTGCCAGCAATAGt                            <  2:758771/96‑1 (MQ=255)
                                                                          ccTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAAtt                       <  2:697486/100‑1 (MQ=255)
                                                                          ccTACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAAtt                       <  1:1434440/100‑1 (MQ=255)
                                                                            tACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTa                      >  1:2003827/1‑100 (MQ=255)
                                                                            tACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTAc                     >  2:1574532/1‑100 (MQ=255)
                                                                            tACATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTAc                     >  1:1248433/1‑100 (MQ=255)
                                                                             aCATTTCCTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACg                    <  2:383125/100‑1 (MQ=255)
                                                                              cATTTCCTTGCAATATGTGCATACTTTGTAGTCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACgg                   <  1:2349136/100‑1 (MQ=255)
                                                                                   ccTTGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAAGAGTGAATTACGGCTTCg              <  1:1272246/100‑1 (MQ=255)
                                                                                     ttGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc            >  1:2520816/1‑100 (MQ=255)
                                                                                     ttGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc            >  1:370350/1‑100 (MQ=255)
                                                                                     ttGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc            <  1:453590/100‑1 (MQ=255)
                                                                                     ttGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc            <  1:758064/100‑1 (MQ=255)
                                                                                     ttGCAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc            <  2:589834/100‑1 (MQ=255)
                                                                                      tGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc            >  1:1404850/1‑100 (MQ=255)
                                                                                       gCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCca           <  1:2366547/100‑1 (MQ=255)
                                                                                        cAATATGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCcaca         >  2:1563547/1‑100 (MQ=255)
                                                                                            atGTGCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAc     <  2:2168081/100‑1 (MQ=255)
                                                                                             tgtgCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc    >  2:1429837/1‑100 (MQ=255)
                                                                                              gtgCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc    <  1:1459213/100‑1 (MQ=255)
                                                                                              gtgCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCa   >  1:1098446/1‑100 (MQ=255)
                                                                                              gtgCATACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCa   >  2:927798/1‑100 (MQ=255)
                                                                                                gCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCaa  >  2:936056/1‑100 (MQ=255)
                                                                                                   |                                                                                                
ATAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCAA  >  NC_000913/3512461‑3512656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: