Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,053,516:1 (G)6→7 100% coding (2663/2745 nt) torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,053,5101.G66.3% 186.1 / 120.2 86coding (2669/2745 nt)torShybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base . (17/12);  new base G (30/27);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 104 total aligned reads displayed.

GTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGC  >  NC_000913/1053411‑1053604
                                                                                                     |                                                                                                 
gttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGACCGACAACACTGCGTGTAATTTCTTCGTGTGGaa                                                                                                    >  1:1314982/1‑100 (MQ=255)
gttCTTAACTGGCTGACATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGaa                                                                                                    >  2:2315373/1‑100 (MQ=255)
 ttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGaa                                                                                                    >  1:1778104/1‑99 (MQ=255)
 ttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAg                                                                                                  <  2:1521521/100‑1 (MQ=255)
   cTTAACTGGCTGATATTGATTCAAATCGCGTTTATGTTTTTCTTATGCAACCATGCTTCCAGAGAGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggg                                                                                                <  1:671561/100‑1 (MQ=255)
    ttAACTGGCTGATATTGATTCACATCGCGTTCAGGTCCTTCTTATGCCACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggg                                                                                              <  2:1787835/100‑1 (MQ=255)
     tAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggggg                                                                                            >  1:741833/1‑100 (MQ=255)
     tAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggggg                                                                                            >  2:886029/1‑100 (MQ=255)
     tAACTGGCTGATATCGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACCCTGCGTGTAATTTCTTCGTGTGGAAgcggg                                                                                            <  2:1578748/100‑1 (MQ=255)
      aaCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggg                                                                                          <  2:1962687/100‑1 (MQ=255)
      aaCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggg                                                                                          >  2:1776868/1‑100 (MQ=255)
       aCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggggggn                                                                                         <  2:900651/100‑2 (MQ=255)
       aCTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGc                                                                                         <  1:167799/100‑1 (MQ=255)
        cTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggggc                                                                                        <  2:741826/100‑3 (MQ=255)
        cTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggggc                                                                                        >  1:570570/1‑98 (MQ=255)
        cTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCa                                                                                        >  1:2576128/1‑100 (MQ=255)
           gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggggcaga                                                                                     >  2:34841/1‑95 (MQ=255)
           gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggggcaga                                                                                     <  2:2093179/100‑6 (MQ=255)
            cTGTTATTGATTCAAATCGCTTTTAGGTTTTTCTTATGTAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgggggggcaga                                                                                     <  1:1055395/99‑6 (MQ=255)
            cTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGAt                                                                                     >  1:799373/1‑100 (MQ=255)
             taatatTGAGTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTGCGTGTGGAAGGGGGGGCAGATa                                                                                    <  1:771930/98‑1 (MQ=255)
              gATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATaa                                                                                   >  1:2347970/1‑100 (MQ=255)
                tatTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAAt                                                                                  >  2:563908/1‑99 (MQ=255)
                 tctGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCTTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgc                                                                               <  2:1416176/98‑1 (MQ=255)
                 atTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgc                                                                               <  1:1373635/100‑1 (MQ=255)
                  ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgc                                                                               <  1:1501252/100‑1 (MQ=255)
                   tGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgct                                                                              >  2:1645196/1‑100 (MQ=255)
                   tGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgct                                                                              >  2:450931/1‑100 (MQ=255)
                    gATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctg                                                                             <  2:1304043/100‑1 (MQ=255)
                    gATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctg                                                                             >  1:1664962/1‑100 (MQ=255)
                    gATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgcaga                                                                            >  1:1416190/1‑97 (MQ=255)
                     aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGCAGGGGGGGCAGATAATGgctgc                                                                            <  2:1533612/100‑1 (MQ=255)
                     aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgc                                                                            >  2:1574015/1‑100 (MQ=255)
                     aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgc                                                                            <  1:2383241/100‑1 (MQ=255)
                     aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgctgct                                                                           <  1:1256030/100‑1 (MQ=255)
                      ttCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctggt                                                                           >  2:666784/1‑98 (MQ=255)
                      ttCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgct                                                                           <  2:1089444/100‑1 (MQ=255)
                      ttCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGAGTAATTTCTTCAAGTGAAAGGGAGGGCAGAAAATGgctgct                                                                           >  1:2387121/1‑100 (MQ=255)
                       tCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgctgctgc                                                                         <  2:1294291/100‑1 (MQ=255)
                        cAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgctgc                                                                         <  2:521252/100‑1 (MQ=255)
                        cAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgctgc                                                                         >  2:1662039/1‑100 (MQ=255)
                        cAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgctgctgct                                                                        >  1:2018008/1‑100 (MQ=255)
                           aTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTcc                                                                      <  1:311773/100‑1 (MQ=255)
                           aTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTcc                                                                      >  1:2510045/1‑100 (MQ=255)
                           aTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCa                                                                     >  2:1476801/1‑100 (MQ=255)
                           aTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCa                                                                     >  2:1476066/1‑100 (MQ=255)
                            tCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAg                                                                    <  2:1917666/100‑1 (MQ=255)
                              gcgTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGt                                                                   <  2:1271178/100‑1 (MQ=255)
                                gTTCAGGACTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAAGGGGGGCAGATAATGGCTGCTGCTCCAGTtg                                                                 >  1:1392283/1‑100 (MQ=255)
                                 ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTtgtg                                                               <  2:683080/100‑1 (MQ=255)
                                    aGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgcg                                                             <  1:1873096/100‑1 (MQ=255)
                                     ggTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgcgc                                                            <  1:1582144/99‑1 (MQ=255)
                                        ctttctcATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATa                                                         >  1:496704/1‑100 (MQ=255)
                                          ttcttATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgct                                                      >  1:1855640/1‑100 (MQ=255)
                                              tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                   >  2:400801/1‑100 (MQ=255)
                                              tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGAGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                   >  1:805648/1‑100 (MQ=255)
                                                tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTTCGCATAgctggct                                                  >  1:1302592/1‑99 (MQ=255)
                                                tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggctg                                                 >  2:2445011/1‑100 (MQ=255)
                                                 gCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGctggctgg                                                >  2:2026007/1‑100 (MQ=255)
                                                  cAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCg                                              >  1:1484987/1‑100 (MQ=255)
                                                      cATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGata                                           <  2:339052/100‑1 (MQ=255)
                                                      cATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGata                                           >  2:2619731/1‑100 (MQ=255)
                                                        tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATg                                         >  1:2483349/1‑100 (MQ=255)
                                                          cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCa                                       >  2:2510112/1‑100 (MQ=255)
                                                          cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCAt                                      <  2:316551/100‑1 (MQ=255)
                                                              cAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc                                   >  2:2334136/1‑100 (MQ=255)
                                                                gagCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa                                 >  1:2236496/1‑100 (MQ=255)
                                                                gagCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                >  1:756575/1‑100 (MQ=255)
                                                                 agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                <  1:2421041/100‑1 (MQ=255)
                                                                    ggCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGGTGGCTGGCGATATGCATTCCCAGACt                             >  2:479850/1‑100 (MQ=255)
                                                                    ggCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGTGGCAGATAATGGCTGCTGCTCCAGTTGTGCTCATAGCTGGCTGGCGATATGCATTCCCa                                 <  2:2387091/96‑1 (MQ=255)
                                                                    ggCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGAGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACt                             >  2:1850010/1‑100 (MQ=255)
                                                                    ggCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTc                            >  2:469374/1‑100 (MQ=255)
                                                                    ggCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTc                            >  2:1892949/1‑100 (MQ=255)
                                                                     gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTc                            <  2:1778087/100‑1 (MQ=255)
                                                                      cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCg                           >  1:1726531/1‑100 (MQ=255)
                                                                      cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGa                          <  1:922894/100‑1 (MQ=255)
                                                                         cacTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGc                        <  1:550034/100‑1 (MQ=255)
                                                                          acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCa                       <  1:1776885/100‑1 (MQ=255)
                                                                          acTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                      >  1:2355184/1‑100 (MQ=255)
                                                                             gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACt                    <  2:2479737/100‑1 (MQ=255)
                                                                                tgtAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCt                 >  1:1087177/1‑100 (MQ=255)
                                                                                tgtAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCt                 <  2:1308240/100‑1 (MQ=255)
                                                                                tgtAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCtt                >  2:1717279/1‑100 (MQ=255)
                                                                                 gtAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCtt                >  1:1021655/1‑100 (MQ=255)
                                                                                    aTTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCTAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCttttt             <  1:1304035/100‑1 (MQ=255)
                                                                                    aTTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCttttt             >  2:2659441/1‑100 (MQ=255)
                                                                                     tttCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTa            <  2:2349900/100‑1 (MQ=255)
                                                                                     tttCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTACTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTa            >  2:1416406/1‑100 (MQ=255)
                                                                                      ttcttcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAg           <  2:463254/100‑1 (MQ=255)
                                                                                      ttcttcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAg           >  2:1679144/1‑100 (MQ=255)
                                                                                      ttcttcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAg           <  2:2495698/100‑1 (MQ=255)
                                                                                       tcttcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGc          <  1:419165/100‑1 (MQ=255)
                                                                                        cttcGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTg        <  1:1370568/100‑1 (MQ=255)
                                                                                         ttcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCTATTTGTGTGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTg        <  1:392294/100‑1 (MQ=255)
                                                                                              gtgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcg   <  2:1154985/100‑1 (MQ=255)
                                                                                              gtgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcg   >  1:1658926/1‑100 (MQ=255)
                                                                                              gtgGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGCTgcgc  >  2:1315288/1‑100 (MQ=255)
                                                                                               tgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcgc  >  1:1651138/1‑100 (MQ=255)
                                                                                               tgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATgcgc  >  2:18002/1‑100 (MQ=255)
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GTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGC  >  NC_000913/1053411‑1053604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: