Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,652,855:1 (G)6→7 100% coding (362/846 nt) sseA → 3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,652,8491.G96.8% 324.5 / 4.0 95coding (356/846 nt)sseA3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (51/41);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

Only 100 of 104 total aligned reads displayed.

AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAT  >  NC_000913/2652750‑2652943
                                                                                                    |                                                                                                 
aGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCt                                                                                                    >  1:124542/1‑100 (MQ=255)
aGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATAGTGGATACTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCt                                                                                                    >  1:750489/1‑100 (MQ=255)
 ggATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCt                                                                                                    <  1:380666/99‑1 (MQ=255)
    tAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggg                                                                                              <  2:2327498/100‑1 (MQ=255)
       gCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggg                                                                                          >  1:774986/1‑99 (MQ=255)
       gCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATCCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑ggggggg                                                                                          >  2:1861564/1‑99 (MQ=255)
        cACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggt                                                                                         >  1:1043623/1‑98 (MQ=255)
         aCCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggtg                                                                                        >  1:1287616/1‑97 (MQ=255)
           cTGATTGTCTATGACGAAGGTAATCTTGTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑gggggggttga                                                                                      <  2:753091/100‑6 (MQ=255)
             gATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACt                                                                                    >  2:274333/1‑100 (MQ=255)
              aTTGTCTATGACTAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGAGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACtt                                                                                   <  1:583155/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGc                                                                                 <  2:1925422/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGc                                                                                 <  2:1560457/100‑1 (MQ=255)
                tGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATAGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGGGGACTTGc                                                                                 >  2:912118/1‑100 (MQ=255)
                 gtttATGACGACTGTAATCGTTTCTCTGCCGTATGAGCCTGGTGGATGCTGCGCATCTTTGGTGTAGAGATAGTGTCGATTATGGGGGGGTGGAc                                                                                     <  2:1668698/92‑1 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAgg                                                                              >  2:1607692/1‑100 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAgg                                                                              >  2:1496686/1‑100 (MQ=255)
                   cTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAgg                                                                              >  2:1798016/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             >  2:2305993/1‑100 (MQ=255)
                    tATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTATAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGc                                                                             >  1:224649/1‑100 (MQ=255)
                      tGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTg                                                                           >  1:80726/1‑100 (MQ=255)
                      tGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTg                                                                           >  1:1866640/1‑100 (MQ=255)
                      tGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTg                                                                           <  2:521464/100‑1 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                         <  2:124541/100‑1 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                         <  2:236889/100‑1 (MQ=255)
                        aCGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGc                                                                         >  1:495487/1‑100 (MQ=255)
                          gAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAg                                                                       <  1:2157088/100‑1 (MQ=255)
                             ggTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAgcgc                                                                    <  1:578831/100‑1 (MQ=255)
                             ggTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAgcgc                                                                    >  1:23540/1‑100 (MQ=255)
                                aaTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgat                                                                 >  1:1039421/1‑100 (MQ=255)
                                aaTCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgat                                                                 >  1:2171148/1‑100 (MQ=255)
                                 aTCTTTTCTCAGCCCCACGAGCATGGTGGATGTTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatg                                                                >  1:1441289/1‑100 (MQ=255)
                                   cTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCgatgat                                                              >  2:1009232/1‑100 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             >  1:1807990/1‑100 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  2:1829166/100‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  1:351161/100‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  1:2621773/100‑1 (MQ=255)
                                    ttttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTGTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATc                                                             <  2:1942504/100‑1 (MQ=255)
                                     tttCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTTCAGGCTGGCAGCGCGATGATct                                                            <  2:1542417/100‑1 (MQ=255)
                                        ctctGCCCCACGAGCATGGTGGATGCTGCGCACTTTTGGTGTAGAGAAAGTGTCGAGTCTGGGGGGGGGGACTGGCAGGGTGGCAGCGCTATGAGctgc                                                          >  2:651539/1‑99 (MQ=255)
                                        ctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGAtcttct                                                         >  2:501758/1‑100 (MQ=255)
                                        ctcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgct                                                         >  1:2281012/1‑100 (MQ=255)
                                         tcATCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        <  1:2355310/100‑1 (MQ=255)
                                         tcAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATctgctg                                                        <  2:1054476/100‑1 (MQ=255)
                                          cAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGt                                                       <  1:268366/100‑1 (MQ=255)
                                          cAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGt                                                       >  2:2417272/1‑100 (MQ=255)
                                           aGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGtt                                                      >  1:1729323/1‑100 (MQ=255)
                                            gCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTa                                                     <  1:1586084/100‑1 (MQ=255)
                                            gCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTa                                                     <  1:1534820/100‑1 (MQ=255)
                                            gCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTa                                                     <  1:2306021/100‑1 (MQ=255)
                                             ccccACGAGCATGGTGGATGCTGCGCACCTGTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTa                                                     <  1:449013/99‑1 (MQ=255)
                                              cccACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGCAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTaga                                                   >  2:2541322/1‑100 (MQ=255)
                                                 aCGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTagaaga                                                <  1:912127/100‑1 (MQ=255)
                                                     gCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGt                                            <  1:2209317/100‑1 (MQ=255)
                                                       aTGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGc                                          <  2:495483/100‑1 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                        <  2:2027789/100‑1 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                        <  1:1913299/100‑1 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                        >  1:2073059/1‑100 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAg                                        <  1:373254/100‑1 (MQ=255)
                                                         ggtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGAAGAACTGCTGTTAAAAGAAGGTGCAg                                        >  1:1270210/1‑100 (MQ=255)
                                                          gtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGt                                       <  2:2386212/100‑1 (MQ=255)
                                                          gtggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGt                                       <  1:865824/100‑1 (MQ=255)
                                                           tggATGGTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTa                                      <  2:2109037/100‑1 (MQ=255)
                                                            ggATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACATGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTag                                     >  2:2412550/1‑100 (MQ=255)
                                                              aTGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                   >  2:1072021/1‑100 (MQ=255)
                                                              aTGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTagag                                   <  1:2487659/100‑1 (MQ=255)
                                                                 ctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTg                                >  1:1692087/1‑100 (MQ=255)
                                                                 ctgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTg                                >  1:1649047/1‑100 (MQ=255)
                                                                  tgcGCACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGc                               >  1:824308/1‑100 (MQ=255)
                                                                   gcgcACCTTTGGTGTAGAGAAAGTGTCGCTTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGGTGTTCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGcc                              >  2:1536027/1‑100 (MQ=255)
                                                                   gcgcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGcc                              >  1:1496278/1‑100 (MQ=255)
                                                                   gcgcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGcc                              >  2:1290744/1‑100 (MQ=255)
                                                                    cgcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCg                             <  2:1043613/100‑1 (MQ=255)
                                                                    cgcACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGAGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCg                             <  1:2526547/100‑1 (MQ=255)
                                                                     gcACCTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCg                             <  1:280132/100‑1 (MQ=255)
                                                                     gcACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGCTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCgg                            >  2:1416184/1‑100 (MQ=255)
                                                                        ccTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGAAGAGCTGCCGGAAg                         <  2:153191/100‑1 (MQ=255)
                                                                        ccTTTGGTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAAAAGGTGCAGTAGAGCTGCCGGAAg                         >  1:1015435/1‑100 (MQ=255)
                                                                        ccTTTGGTATAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAg                         >  2:2108527/1‑100 (MQ=255)
                                                                         cTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAgg                        <  2:1626602/99‑1 (MQ=255)
                                                                             ngtgtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGagag                    >  2:1520015/2‑100 (MQ=255)
                                                                             ggTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGagag                    >  1:1586659/1‑100 (MQ=255)
                                                                             ggTGTAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGagag                    >  1:435694/1‑100 (MQ=255)
                                                                                gtAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGttt                 >  1:2220509/1‑100 (MQ=255)
                                                                                 tAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTa                <  2:1807973/100‑1 (MQ=255)
                                                                                 tAGAGAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTa                >  1:1264037/1‑100 (MQ=255)
                                                                                  agagAAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTaa               >  1:1185607/1‑100 (MQ=255)
                                                                                   gagaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAc              <  1:2508603/100‑1 (MQ=255)
                                                                                     gaAAGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcga            >  1:2521640/1‑99 (MQ=255)
                                                                                      aaaGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAAcgcc           >  1:1843996/1‑100 (MQ=255)
                                                                                        aGTGTCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGGAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGGTTAAcgccgc         >  2:2525762/1‑100 (MQ=255)
                                                                                         gtgtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCGGCCGGAAGGAGAGTTTAACGCcgcg        <  1:1290757/100‑1 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      >  1:2601136/1‑100 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGtt      <  2:457041/100‑1 (MQ=255)
                                                                                           gtCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGACGGAAGGAGAGTTTAACGCCGCGtt      >  1:1362757/1‑100 (MQ=255)
                                                                                            tCGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGttt     >  1:721041/1‑100 (MQ=255)
                                                                                             cGATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTa    >  1:2235517/1‑100 (MQ=255)
                                                                                              gATTCTGGGGGGGTGGACTTGCAGGCTGGCAGCTCGATGATCTGCTGTTAGAAAAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTaa   >  1:36248/1‑100 (MQ=255)
                                                                                               aTTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAt  <  1:1861581/100‑1 (MQ=255)
                                                                                               aTTCTGGGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAt  <  1:1649369/100‑1 (MQ=255)
                                                                                                    |                                                                                                 
AGGATAAGCACCTGATTGTCTATGACGAAGGTAATCTTTTCTCAGCCCCACGAGCATGGTGGATGCTGCGCACCTTTGGTGTAGAGAAAGTGTCGATTCT‑‑GGGGGGTGGACTTGCAGGCTGGCAGCGCGATGATCTGCTGTTAGAAGAAGGTGCAGTAGAGCTGCCGGAAGGAGAGTTTAACGCCGCGTTTAAT  >  NC_000913/2652750‑2652943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: