Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,809,774:1 (T)7→8 100% coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7671.T73.8% 282.4 / 102.3 107coding (151/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base . (14/14);  new base T (32/47);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA  >  NC_000913/2809668‑2809860
                                                                                                    |                                                                                               
 gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑t                                                                                               >  1:2361949/1‑100 (MQ=255)
  aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tt                                                                                              >  2:2638678/1‑100 (MQ=255)
     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttt                                                                                          >  1:993222/1‑100 (MQ=255)
     cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttt                                                                                          >  2:1849808/1‑100 (MQ=255)
      aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑tttttt                                                                                         <  2:2538876/100‑1 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCTTTTGTTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttttt                                                                                        <  1:15146/100‑1 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttttt                                                                                        <  2:1499618/100‑1 (MQ=255)
       aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttttt                                                                                        >  2:200650/1‑100 (MQ=255)
         gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttttttc                                                                                      >  2:665156/1‑98 (MQ=255)
          aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGCCCCGTCTGGAATTCTCTCCTCTCAAAAGCG‑‑ttttttttct                                                                                     <  2:1931905/100‑4 (MQ=255)
          aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttttttct                                                                                     <  1:1500378/100‑4 (MQ=255)
           cAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTcc                                                                                    <  2:2150886/100‑1 (MQ=255)
            aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑ttttttttct                                                                                     <  2:469931/98‑4 (MQ=255)
             gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCt                                                                                   >  1:2335142/1‑100 (MQ=255)
               ttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGc                                                                                 >  2:612751/1‑100 (MQ=255)
                 aCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGcatc                                                                              >  2:301156/1‑100 (MQ=255)
                   cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatca                                                                             <  2:1000265/100‑1 (MQ=255)
                   cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatca                                                                             <  1:1849825/100‑1 (MQ=255)
                    gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatcat                                                                            <  1:2423652/100‑1 (MQ=255)
                    gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatcat                                                                            >  1:1039235/1‑100 (MQ=255)
                    gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCAtt                                                                           >  2:2511000/1‑100 (MQ=255)
                     aTTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCAtt                                                                           <  1:28798/100‑1 (MQ=255)
                      ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCAttt                                                                          >  2:604097/1‑100 (MQ=255)
                       tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTa                                                                         <  1:2096672/100‑1 (MQ=255)
                       tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTCTCCTGCATCATTTATg                                                                       <  2:992759/100‑1 (MQ=255)
                           attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCa                                                                     <  1:612755/100‑1 (MQ=255)
                            ttaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATTCa                                                                     >  2:1042720/1‑99 (MQ=255)
                             taGTTATATTCCGGTGGCCTTTGCGTTTGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAgg                                                                   <  1:141389/100‑1 (MQ=255)
                             taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTGTTTCTCCTGCATCATTTATGCAgg                                                                   >  1:491792/1‑100 (MQ=255)
                             taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGgc                                                                  <  1:2511030/100‑1 (MQ=255)
                             taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCATCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAgg                                                                   >  1:2036469/1‑100 (MQ=255)
                              aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGgc                                                                  >  2:236131/1‑100 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGgcg                                                                 >  1:2243360/1‑100 (MQ=255)
                               gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATAAATGATGCAggaga                                                                <  2:2597524/100‑4 (MQ=255)
                                ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAggaga                                                                <  1:424654/100‑4 (MQ=255)
                                ttATATTCCGGCGCCTTTTCCGTTCGGTCTCAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATTCAGgcgc                                                                <  2:2133049/100‑1 (MQ=255)
                                 tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTTCATCATTTATGCAGgc                                                                  >  2:1874078/1‑97 (MQ=255)
                                  atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAggagaaa                                                              <  2:2239487/100‑6 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGc                                                            <  1:948181/100‑1 (MQ=255)
                                    atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           >  1:926814/1‑100 (MQ=255)
                                     ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  2:179895/100‑1 (MQ=255)
                                      tCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                         >  2:917287/1‑100 (MQ=255)
                                       ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                        <  1:301158/100‑1 (MQ=255)
                                            gaccTTTGAGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAGGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGt                                                    <  2:1360676/98‑1 (MQ=255)
                                             gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTACATCATTTATGCATGCACGAACCAGTTAGt                                                    >  1:2544725/1‑99 (MQ=255)
                                                   gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                            <  1:30879/100‑1 (MQ=255)
                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                            >  2:1905266/1‑100 (MQ=255)
                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                            >  1:2590597/1‑100 (MQ=255)
                                                    cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                           <  1:823441/100‑1 (MQ=255)
                                                     gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                           >  1:1780309/1‑100 (MQ=255)
                                                      ttCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgcg                                          <  1:1782915/100‑1 (MQ=255)
                                                          gTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                     >  1:164279/1‑100 (MQ=255)
                                                           tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAAGCGCGAACCAGTTCGTCATTACCGCGATGCt                                     >  1:1064363/1‑100 (MQ=255)
                                                            tctaaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   <  2:838483/97‑1 (MQ=255)
                                                            cTGAATGCGCCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                    <  2:1848408/100‑1 (MQ=255)
                                                             tcaaTGCTACCCGTCTGGCATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   <  2:2096640/98‑1 (MQ=255)
                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   <  2:2361919/100‑1 (MQ=255)
                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   <  2:1317230/100‑1 (MQ=255)
                                                             tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                  <  1:246857/100‑1 (MQ=255)
                                                              gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   >  1:1707423/1‑100 (MQ=255)
                                                               aaTGCGACCCGTCTGGGATTCTCTCCTCTTGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                 <  2:223526/100‑1 (MQ=255)
                                                               aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTggaga                                >  1:838492/1‑97 (MQ=255)
                                                                aTGCGACCCGTCTGGGATTCTTTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                <  1:2542075/100‑1 (MQ=255)
                                                                aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                >  2:1270654/1‑100 (MQ=255)
                                                                aTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg                                >  1:162724/1‑100 (MQ=255)
                                                                    gACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCTGGCGCGAGCCTGTTCGTCATTACCGCGATGCTGGCAGCCgtg                           <  1:1792437/100‑3 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                           <  2:2544695/100‑1 (MQ=255)
                                                                     aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                           <  1:2110034/100‑1 (MQ=255)
                                                                      cccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                          >  1:1149317/1‑100 (MQ=255)
                                                                       ccttttGGGGTTTTCTCCTCTCCGAGGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                          <  2:339384/94‑1 (MQ=255)
                                                                         gTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagta                       >  1:2585600/1‑100 (MQ=255)
                                                                            tGGGATTCTCTCCTCTCGAAATCGTTTTTTTTCTCCGGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                    <  1:1866754/100‑1 (MQ=255)
                                                                             gggATTCTCTCCTCTCGAAAGCG‑‑TATTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                 >  2:607713/1‑100 (MQ=255)
                                                                              ggATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtg                  <  2:2612031/100‑1 (MQ=255)
                                                                              ggATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                 >  2:977475/1‑100 (MQ=255)
                                                                               gATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGTCGCGAGCCAGTTCGTCATTACCGCGATGCTAGCAGCAGGGAATAGTTtgt                 >  1:388908/1‑100 (MQ=255)
                                                                               gATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  2:1585209/100‑1 (MQ=255)
                                                                                aTTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  2:1087628/100‑1 (MQ=255)
                                                                                aTTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  2:1019879/100‑1 (MQ=255)
                                                                                aTTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  1:1502246/100‑1 (MQ=255)
                                                                                 ttttcttctttcTAAAGTG‑‑TTTTTTTTTTGTGTATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg               <  1:969768/96‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  2:2492417/99‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg               <  1:1879632/100‑1 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg               >  1:1013050/1‑100 (MQ=255)
                                                                                 ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCATGAATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg               >  2:1743150/1‑100 (MQ=255)
                                                                                   ctctcttctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg               <  2:2018494/98‑1 (MQ=255)
                                                                                   ctctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt             >  1:2165057/1‑100 (MQ=255)
                                                                                   ctctcctctcGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt            >  1:2313405/1‑100 (MQ=255)
                                                                                     ctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtg           >  2:347640/1‑100 (MQ=255)
                                                                                      tcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc          <  2:520716/100‑1 (MQ=255)
                                                                                       cctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct         >  1:1117662/1‑100 (MQ=255)
                                                                                       cctctcGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        >  1:2393184/1‑100 (MQ=255)
                                                                                        ctctgtaaaGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct         <  2:2149155/93‑1 (MQ=255)
                                                                                        ctctcGAATGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct         <  2:1149307/99‑1 (MQ=255)
                                                                                        ctctcGAAAGCGTTTTTTTTCTCCTGTATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  1:1042730/100‑1 (MQ=255)
                                                                                        ctctcGAAAGCGTTTTTTTTCTCCTGCATTATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  1:200652/100‑1 (MQ=255)
                                                                                        ctctcGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCTAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc       <  1:164540/100‑1 (MQ=255)
                                                                                          ctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa      >  2:2193474/1‑100 (MQ=255)
                                                                                            cGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt    <  2:380742/100‑1 (MQ=255)
                                                                                              aaaGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt    <  2:2331185/98‑1 (MQ=255)
                                                                                                    |                                                                                               
GGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCG‑‑TTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA  >  NC_000913/2809668‑2809860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: