Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 432,193:1 +G 100% intergenic (‑180/+119) tsx ← / ← yajI nucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913432,1891.G64.9% 283.8 / 197.7 134intergenic (‑176/+123)tsx/yajInucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (27/20);  new base G (38/47);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 149 total aligned reads displayed.

AGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACG  >  NC_000913/432093‑432288
                                                                                                   |                                                                                                    
agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcggg                                                                                                  <  1:2560155/100‑1 (MQ=255)
agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcggg                                                                                                  <  2:1902952/100‑1 (MQ=255)
agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcggg                                                                                                  <  2:1475982/100‑1 (MQ=255)
 gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACTGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                                 <  2:2278479/100‑1 (MQ=255)
 gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                                 <  2:2012949/100‑1 (MQ=255)
 gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                                 <  1:1172101/100‑1 (MQ=255)
 gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                                 <  1:1285336/100‑1 (MQ=255)
     attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCAATTGCGTTCACGTTTACACACATTCGGGCgggggatt                                                                                             >  1:248702/1‑96 (MQ=255)
        ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTa                                                                                           <  1:2354128/100‑1 (MQ=255)
         taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCCTTTACGTAATCGATTGCGTTCAAGTTTACACACATTCGAGCGGGGATTGTACt                                                                                         >  2:2477643/1‑100 (MQ=255)
         taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTAc                                                                                          >  1:1362215/1‑100 (MQ=255)
         taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGAGGGATTGTAc                                                                                          <  2:1600860/100‑1 (MQ=255)
          naaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACAAACATTCGGGAGGGGATTGTACTg                                                                                        >  2:2287378/2‑100 (MQ=255)
          aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACt                                                                                         >  2:1121900/1‑100 (MQ=255)
          aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACt                                                                                         >  1:621905/1‑100 (MQ=255)
          aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTg                                                                                        >  1:1414889/1‑100 (MQ=255)
          aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTg                                                                                        >  1:755891/1‑100 (MQ=255)
             aaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGAc                                                                                      >  2:237532/1‑100 (MQ=255)
             aaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGAc                                                                                      >  2:221121/1‑100 (MQ=255)
                 aTTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTc                                                                                  >  2:925029/1‑100 (MQ=255)
                 aTTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCg                                                                                 >  1:1657311/1‑100 (MQ=255)
                   tGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGcc                                                                               <  1:111133/100‑1 (MQ=255)
                     cTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGccc                                                                              >  1:436380/1‑100 (MQ=255)
                     cTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCt                                                                             >  2:2633006/1‑100 (MQ=255)
                      tCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCtt                                                                            >  1:345408/1‑100 (MQ=255)
                       cacaACAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCtt                                                                            >  2:550787/1‑100 (MQ=255)
                       cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCtt                                                                            >  1:2264309/1‑100 (MQ=255)
                       cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCtt                                                                            >  2:1349046/1‑100 (MQ=255)
                       cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCt                                                                             >  1:581911/1‑99 (MQ=255)
                       cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGAGCGGGGGATTGTACTGACTTTCGCCCtt                                                                            >  2:548094/1‑100 (MQ=255)
                        acaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCtt                                                                            <  1:1717163/99‑1 (MQ=255)
                         caAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCttgtt                                                                         >  1:505156/1‑100 (MQ=255)
                          ttccgatcttttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCttgttg                                                                        <  1:2337892/91‑1 (MQ=255)
                           aaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttgttg                                                                        <  1:111210/100‑1 (MQ=255)
                           aaaaTATGTGTCGTTTCACAGTTCTATCTTTTACGTAATCGATTGCGTTCACTTTTACACACCTTCGGGCTGGGTATTGTACTGACTTTCGCCCttgttg                                                                        <  2:1285727/100‑1 (MQ=255)
                            aaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGc                                                                       <  2:2115728/100‑1 (MQ=255)
                             aaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCa                                                                      >  1:2074335/1‑100 (MQ=255)
                               tatGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCa                                                                      <  1:245904/97‑1 (MQ=255)
                                 tGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACt                                                                  <  1:873328/100‑1 (MQ=255)
                                  gtttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtt                                                                 >  1:1080915/1‑100 (MQ=255)
                                   tttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGCCTTTCGCCCttgttgagatcggaa                                                               >  2:2337862/1‑91 (MQ=255)
                                    ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTa                                                              <  1:2456105/100‑1 (MQ=255)
                                      cgtttcACAGTTCTATCATTTACGTAATCTATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                             <  2:1594439/100‑1 (MQ=255)
                                      cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                             <  2:2151221/100‑1 (MQ=255)
                                      cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                             <  1:769668/100‑1 (MQ=255)
                                      cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                             <  1:804450/100‑1 (MQ=255)
                                       gtttcACAGTTCTATCATTTACGTAATCGATTGCTTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTa                                                           <  1:1385169/100‑1 (MQ=255)
                                       gtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCTGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACt                                                            >  1:1320382/1‑100 (MQ=255)
                                       gtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACt                                                            <  2:1216935/100‑1 (MQ=255)
                                       gtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACt                                                            >  2:658013/1‑100 (MQ=255)
                                        tttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTa                                                           <  1:218535/100‑1 (MQ=255)
                                        tttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTAt                                                          >  1:1235496/1‑100 (MQ=255)
                                          tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATc                                                         >  2:2574192/1‑100 (MQ=255)
                                          tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCa                                                        <  1:882791/100‑1 (MQ=255)
                                           cacaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCaa                                                       >  1:2130053/1‑100 (MQ=255)
                                            acaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCaa                                                       >  1:2443769/1‑100 (MQ=255)
                                             caGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAAt                                                      <  1:934599/100‑1 (MQ=255)
                                             caGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAAt                                                      <  1:318031/100‑1 (MQ=255)
                                                  cTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTTGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAgg                                                 <  2:1866012/100‑1 (MQ=255)
                                                  cTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGAGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAg                                                  >  2:1175566/1‑99 (MQ=255)
                                                      cATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGAt                                             <  1:569737/100‑1 (MQ=255)
                                                        tttACGTAATCGATTGCGTTCACGTTTACACACCTTCGGGCGGTGATTGTACTGACTTTCACCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTa                                          >  1:1502750/1‑100 (MQ=255)
                                                         ttACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTa                                          <  2:1643258/100‑1 (MQ=255)
                                                          tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAg                                        <  1:1769824/100‑1 (MQ=255)
                                                            cGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGc                                       <  2:2443739/100‑1 (MQ=255)
                                                             ttAATCGATTGCGTTCACGTTTACACATATTCGTGTGGGGGATTGTACTGACTTTCTCCCTTGTTGCAAACTTTTACTATCAATCCAGGTTATGTAAGc                                       <  1:1197268/98‑1 (MQ=255)
                                                             gTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCa                                      <  2:219607/100‑1 (MQ=255)
                                                             gTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCa                                      >  1:246932/1‑100 (MQ=255)
                                                             gTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCa                                      <  2:1383096/100‑1 (MQ=255)
                                                              tAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCag                                     >  2:732237/1‑100 (MQ=255)
                                                              tAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCag                                     <  2:416608/100‑1 (MQ=255)
                                                              tAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCag                                     <  2:581907/100‑1 (MQ=255)
                                                              tAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTACAAACTTTTACTATCAATCCAGGTGATGTAAGCaga                                    >  1:205647/1‑100 (MQ=255)
                                                               aaTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCaga                                    <  1:518942/100‑1 (MQ=255)
                                                                 tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGt                                  <  2:2536515/100‑1 (MQ=255)
                                                                  cGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTa                                 <  2:1776636/100‑1 (MQ=255)
                                                                  cGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTACAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTaa                                >  1:1499425/1‑100 (MQ=255)
                                                                   gATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAAAGTaa                                >  1:879111/1‑100 (MQ=255)
                                                                     ttGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACa                              <  2:2261039/100‑1 (MQ=255)
                                                                       gCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACaaaa                           >  2:394912/1‑100 (MQ=255)
                                                                        cGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAAt                          >  2:1423984/1‑100 (MQ=255)
                                                                        cGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAAt                          >  1:2263127/1‑100 (MQ=255)
                                                                        cGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAAt                          >  2:37084/1‑100 (MQ=255)
                                                                         gTTCACGTTTACACACATTTGGGCGGGGGCTTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCTGAGTAACAAAAt                          >  2:328251/1‑100 (MQ=255)
                                                                         gTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATg                         >  1:261701/1‑100 (MQ=255)
                                                                          ttCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCCGGTGATGTAAGCAGAGTACCAAAAta                         >  2:1810726/1‑99 (MQ=255)
                                                                           tCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGAc                       >  2:1956202/1‑100 (MQ=255)
                                                                              cGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACggg                    >  1:1505990/1‑100 (MQ=255)
                                                                              cGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACggg                    >  2:262393/1‑100 (MQ=255)
                                                                                 ttACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGa                  >  1:1001103/1‑100 (MQ=255)
                                                                                 ttACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGa                  <  2:2074312/100‑1 (MQ=255)
                                                                                 ttACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGa                  >  1:1001052/1‑100 (MQ=255)
                                                                                 ttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaa                 <  1:1236503/100‑1 (MQ=255)
                                                                                   acacacATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGAAGGGGaaa                >  2:1106541/1‑100 (MQ=255)
                                                                                   acacacATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg               <  1:835236/100‑1 (MQ=255)
                                                                                    cacacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg               <  1:1598726/100‑1 (MQ=255)
                                                                                    cacacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg               <  2:1713444/100‑1 (MQ=255)
                                                                                     acacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGa              <  1:2445277/100‑1 (MQ=255)
                                                                                      cacaTTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATg            <  2:1509982/100‑1 (MQ=255)
                                                                                            cgggcgggGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCg       >  2:1010818/1‑100 (MQ=255)
                                                                                                   |                                                                                                    
AGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACG  >  NC_000913/432093‑432288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: