Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258676 258683 8–776 116 [0] [1] 115 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)92 (1.040) 57/162 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 115 total aligned reads displayed.

GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGC  >  NC_000913/258586‑258782
                                                                                                  |                                                                                                  
atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                   <  1:101687‑M2/2‑1 (MQ=255)
atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                   >  1:1349562‑M2/99‑100 (MQ=255)
  cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                 <  1:1935610‑M2/4‑1 (MQ=255)
   acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                <  2:844197‑M2/5‑1 (MQ=255)
    caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAGTGc                                                                                                <  2:1049528‑M2/5‑1 (MQ=255)
      acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                             <  2:1824925‑M2/8‑1 (MQ=255)
      acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                             >  1:254077‑M2/93‑100 (MQ=255)
       caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                            <  1:2208863‑M2/9‑1 (MQ=255)
        aggagatagcaatgacgttaccgagtggacacgcgaagaacagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                           <  2:1863460‑M2/10‑1 (MQ=255)
        aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                           <  1:1124266‑M2/10‑1 (MQ=255)
         ggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                          >  2:2257295‑M2/90‑100 (MQ=255)
              tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                     >  1:1807133‑M2/85‑100 (MQ=255)
              tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAAATAATCg                                                                                     >  1:477047‑M2/85‑100 (MQ=255)
                gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                   >  1:2109850‑M2/83‑100 (MQ=255)
                gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                   >  2:1098681‑M2/83‑100 (MQ=255)
                gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                   >  2:2084131‑M2/83‑100 (MQ=255)
                gcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                   >  2:1395535‑M2/83‑100 (MQ=255)
                 caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATGATCGAtt                                                                                  <  1:1392474‑M2/19‑1 (MQ=255)
                 caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                  <  2:1510391‑M2/19‑1 (MQ=255)
                   atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                <  1:1248362‑M2/21‑1 (MQ=255)
                   atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                <  2:1104404‑M2/21‑1 (MQ=255)
                       cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAgacaatcgaaaaagaat                                                                            <  1:1893171‑M2/25‑9 (MQ=255)
                        gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                           <  2:211878‑M2/26‑1 (MQ=255)
                        gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                           <  1:530543‑M2/26‑1 (MQ=255)
                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                          <  1:2633515‑M2/27‑1 (MQ=255)
                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                          <  1:1972950‑M2/27‑1 (MQ=255)
                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                          <  1:2162106‑M2/27‑1 (MQ=255)
                          taccgagtggacacccgaagagcagattgatcaaaatatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                         >  1:1872953‑M2/73‑100 (MQ=255)
                           accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                        >  2:1338429‑M2/72‑100 (MQ=255)
                             cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                      >  1:314208‑M2/70‑100 (MQ=255)
                               agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                    >  1:1435647‑M2/68‑100 (MQ=255)
                                 tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTg                                                                  <  2:2109727‑M2/35‑1 (MQ=255)
                                 tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTg                                                                  <  1:2264063‑M2/35‑1 (MQ=255)
                                  tctcacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                 <  2:286430‑M2/36‑1 (MQ=255)
                                  ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                 >  1:922424‑M2/65‑100 (MQ=255)
                                  ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                 >  2:2418325‑M2/65‑100 (MQ=255)
                                   gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                >  1:2401784‑M2/64‑100 (MQ=255)
                                   gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                >  2:1355095‑M2/64‑100 (MQ=255)
                                    acacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                <  1:1230510‑M2/37‑1 (MQ=255)
                                     cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGtctggaga                                                              >  1:286432‑M2/62‑97 (MQ=255)
                                     cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                              <  1:2329229‑M2/39‑1 (MQ=255)
                                       cccgaagagcaaattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATTATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            <  2:702598‑M2/41‑1 (MQ=255)
                                         cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATg                                                          <  2:254075‑M2/43‑1 (MQ=255)
                                           aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                        <  1:1637274‑M2/45‑1 (MQ=255)
                                           aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                        <  1:1202114‑M2/45‑1 (MQ=255)
                                            agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                       <  1:1974409‑M2/46‑1 (MQ=255)
                                              agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                     >  2:1725150‑M2/53‑100 (MQ=255)
                                              agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                     >  1:2634108‑M2/53‑100 (MQ=255)
                                              agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                     <  2:1252340‑M2/48‑1 (MQ=255)
                                               gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                    >  1:2601285‑M2/52‑100 (MQ=255)
                                               gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAAATAATCGATTCTTTTTCGATTATCTGGCTGTATGCGTCa                                                     >  1:1520904‑M2/52‑99 (MQ=255)
                                                 agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                  <  1:2407032‑M2/51‑1 (MQ=255)
                                                 agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                  <  1:165986‑M2/51‑1 (MQ=255)
                                                  gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                 >  2:833413‑M2/49‑100 (MQ=255)
                                                   attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                >  2:2511763‑M2/48‑100 (MQ=255)
                                                    ttgatcaaaaaatttaccgcactatgcccgtatattcttgaaggtaAGTGCAAACATCATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                               <  2:1792142‑M2/54‑1 (MQ=255)
                                                      gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                             >  2:1490090‑M2/45‑100 (MQ=255)
                                                       atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                            >  2:2361770‑M2/44‑100 (MQ=255)
                                                        tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                           <  1:1024972‑M2/58‑1 (MQ=255)
                                                        tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                           <  1:2410391‑M2/58‑1 (MQ=255)
                                                         caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGCTTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  2:1269698‑M2/42‑100 (MQ=255)
                                                          aaagaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:781899‑M2/41‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGTCTGTATGCGTCAACGTGAAACCgg                                         >  2:550046‑M2/41‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:274053‑M2/41‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  2:1825319‑M2/41‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:1377301‑M2/41‑100 (MQ=255)
                                                          aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGAATGCGTCAACGTGAAACCgg                                         >  1:2203926‑M2/41‑100 (MQ=255)
                                                                tttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCgg                                   <  1:260940‑M2/66‑1 (MQ=255)
                                                                 ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGa                                  <  2:155395‑M2/67‑1 (MQ=255)
                                                                 ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGa                                  >  1:1352504‑M2/34‑100 (MQ=255)
                                                                 ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGa                                  <  1:1489948‑M2/67‑1 (MQ=255)
                                                                  taccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                 >  1:1630698‑M2/33‑100 (MQ=255)
                                                                   accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                <  1:1734232‑M2/69‑1 (MQ=255)
                                                                   accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                <  1:1355108‑M2/69‑1 (MQ=255)
                                                                     cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                              <  2:2136543‑M2/71‑1 (MQ=255)
                                                                        actaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                           >  1:7736‑M2/27‑100 (MQ=255)
                                                                         ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                          <  1:1395549‑M2/75‑1 (MQ=255)
                                                                            ggcccgtagattagttaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTAAACGTGAAACCGGCACAGTAAGTGCgtgga                        >  2:1673749‑M2/23‑97 (MQ=255)
                                                                             gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAtt                      <  2:893355‑M2/79‑1 (MQ=255)
                                                                              cttccgatctcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                     <  1:1025230‑M2/80‑1 (MQ=255)
                                                                              cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                     >  1:57536‑M2/21‑100 (MQ=255)
                                                                              cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                     >  1:1371506‑M2/21‑100 (MQ=255)
                                                                              cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCAATTGTCTGGCTGTATCTGTCATCGTGAAACTGGCACCGGAAGTGGGTg                          >  2:1786244‑M2/21‑95 (MQ=255)
                                                                                cgtatattcgtGAAGGTAAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTTCTGTATGAGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                   >  2:435961/11‑100 (MQ=255)
                                                                                 gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                  <  1:2429232‑M2/83‑1 (MQ=255)
                                                                                  tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAtt                      >  1:504460‑M2/17‑95 (MQ=255)
                                                                                   atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                  <  2:477043‑M2/83‑1 (MQ=255)
                                                                                        cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGtgcgtgaattcagatcggaag           >  2:1025220‑M2/11‑90 (MQ=255)
                                                                                        cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt           >  2:1484586‑M2/11‑100 (MQ=255)
                                                                                        cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt           >  2:709964‑M2/11‑100 (MQ=255)
                                                                                         gtgaaggtaAGTGCAAAGATAATCGATTCTTTGTCGATTGTATGTCTGTATACCTCAACGTGAAACCGGCATCGGAAGTGCGTGAATTCTGGGGCtggtg          >  2:481636‑M2/10‑100 (MQ=255)
                                                                                          tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg         >  2:498191‑M2/9‑100 (MQ=255)
                                                                                          tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg         <  2:2566275‑M2/92‑1 (MQ=255)
                                                                                          tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg         >  2:2430911‑M2/9‑100 (MQ=255)
                                                                                           gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga        <  2:45451‑M2/93‑1 (MQ=255)
                                                                                            aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat       <  2:37098‑M2/94‑1 (MQ=255)
                                                                                              ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg     <  2:2605346‑M2/96‑1 (MQ=255)
                                                                                              ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg     <  1:2083291‑M2/96‑1 (MQ=255)
                                                                                               gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga    >  2:726300‑M2/4‑100 (MQ=255)
                                                                                                 aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGTTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc  <  1:2115861‑M2/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
GAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGC  >  NC_000913/258586‑258782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: