Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,439,189 C→T 16.7% V532V (GTG→GTA)  pfo ← pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,439,1890CT16.7% 194.7 / 29.7 84V532V (GTG→GTA) pfopyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (34/36);  new base T (8/6);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

AGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCAGCCATC  >  NC_000913/1439090‑1439288
                                                                                                   |                                                                                                   
aGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAac                                                                                                     <  2:1876134/100‑1 (MQ=255)
   cATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACg                                                                                                  <  1:1518619/100‑1 (MQ=255)
   cATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTGTTCTGGTTTAACACg                                                                                                  >  1:2610382/1‑100 (MQ=255)
    aTCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACgg                                                                                                 <  1:982118/100‑1 (MQ=255)
    aTCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACgg                                                                                                 <  2:2113878/100‑1 (MQ=255)
     tCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGc                                                                                                <  1:1766499/100‑1 (MQ=255)
      cTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGcc                                                                                               <  2:1721229/100‑1 (MQ=255)
       tGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCt                                                                                              >  2:968796/1‑100 (MQ=255)
       tGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCt                                                                                              >  1:1687049/1‑100 (MQ=255)
       tGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCt                                                                                              <  2:687313/100‑1 (MQ=255)
        gCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTg                                                                                             >  2:2152042/1‑100 (MQ=255)
        gCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTg                                                                                             >  2:614523/1‑100 (MQ=255)
        gCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTg                                                                                             >  2:2053989/1‑100 (MQ=255)
        gCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCAAATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTg                                                                                             >  2:1308185/1‑100 (MQ=255)
         cATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGa                                                                                            <  2:259687/100‑1 (MQ=255)
         cATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGa                                                                                            >  2:1801320/1‑100 (MQ=255)
           tGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAAc                                                                                          >  2:497365/1‑100 (MQ=255)
               ggTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAActtct                                                                                      <  1:2142969/100‑1 (MQ=255)
                gTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAActtctt                                                                                     <  2:573273/100‑1 (MQ=255)
                 tATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTg                                                                                    <  1:873641/100‑1 (MQ=255)
                  aTTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGGGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGc                                                                                   >  2:1439790/1‑100 (MQ=255)
                    tAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCgg                                                                                 >  2:1852970/1‑100 (MQ=255)
                    tAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCACTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCgg                                                                                 <  2:1411630/100‑1 (MQ=255)
                    tAATACGGGCCGCCAGGCCACATTCGCGGGCAATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCgg                                                                                 <  1:963766/100‑1 (MQ=255)
                     aaTACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGc                                                                                >  2:2635538/1‑100 (MQ=255)
                     aaTACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGc                                                                                <  1:1114026/100‑1 (MQ=255)
                       tACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAACGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCaa                                                                              >  1:2171789/1‑100 (MQ=255)
                        aCGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCaa                                                                              >  2:206055/1‑99 (MQ=255)
                        aCGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAg                                                                             >  2:77942/1‑100 (MQ=255)
                          gggCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAgcg                                                                           >  2:10376/1‑100 (MQ=255)
                           ggCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAgcgc                                                                          >  1:326603/1‑100 (MQ=255)
                                  aGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACcaca                                                                   <  2:521353/100‑1 (MQ=255)
                                   ggCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCacac                                                                  >  1:2025770/1‑100 (MQ=255)
                                   ggCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCacac                                                                  >  2:2329952/1‑100 (MQ=255)
                                   ggCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCacac                                                                  >  2:994757/1‑100 (MQ=255)
                                    gCCACATTCGCGGGCGATTTTCGCTGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACt                                                                 <  1:530510/100‑1 (MQ=255)
                                    gCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACt                                                                 <  2:794979/100‑1 (MQ=255)
                                    gCCACATTCGCGGGCGATGTTCGACGAGTTAATCACATAGAAGCGCGATTTTTTCTGGTTTAACACGGAATGAACTTCTTGCGGCAAGCGCGACcaca                                                                   >  2:2420146/1‑98 (MQ=255)
                                       acaTTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCa                                                              <  1:1102845/100‑1 (MQ=255)
                                        caTTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCAt                                                             >  1:886833/1‑100 (MQ=255)
                                        caTTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCAt                                                             >  1:2379261/1‑100 (MQ=255)
                                         aTTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATc                                                            <  2:1365767/100‑1 (MQ=255)
                                            cgcgGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTgc                                                         <  1:1829221/100‑1 (MQ=255)
                                            cgcgGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCACGACCACACTTCATCTgc                                                         >  1:28750/1‑100 (MQ=255)
                                              cgGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTgcgc                                                       >  1:944185/1‑100 (MQ=255)
                                                ggCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTg                                                     <  1:1528452/100‑1 (MQ=255)
                                                 gCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGt                                                    >  2:1454621/1‑100 (MQ=255)
                                                  cGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTa                                                   <  1:904926/100‑1 (MQ=255)
                                                  cGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTACGGCAAGCGCGACCACACTTCATCTGCGCTGTa                                                   >  1:1233762/1‑100 (MQ=255)
                                                   gATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTAc                                                  >  1:2104587/1‑100 (MQ=255)
                                                    aTTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACg                                                 >  1:404486/1‑100 (MQ=255)
                                                    aTTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTAc                                                  >  1:656277/1‑99 (MQ=255)
                                                    aTTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACg                                                 <  1:98081/100‑1 (MQ=255)
                                                      tttCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGc                                               <  1:1728694/100‑1 (MQ=255)
                                                          gccgcGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCgtgt                                           <  2:1929842/100‑1 (MQ=255)
                                                            cgcgTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTg                                         <  2:1277742/100‑1 (MQ=255)
                                                            cgcgTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTg                                         <  2:2148267/100‑1 (MQ=255)
                                                            cgcgTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTg                                         <  1:1454635/100‑1 (MQ=255)
                                                            cgcgTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTg                                         <  2:859118/100‑1 (MQ=255)
                                                             gcgTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGa                                        <  2:886824/100‑1 (MQ=255)
                                                             gcgTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGACAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGa                                        >  1:1002834/1‑100 (MQ=255)
                                                               gTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGc                                      <  1:576781/100‑1 (MQ=255)
                                                                   aTCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGa                                  <  1:517237/100‑1 (MQ=255)
                                                                   aTCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGAGCTGTACGGCGTGTTGAGCAGGa                                  >  1:1208550/1‑100 (MQ=255)
                                                                    tCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGaa                                 <  1:1817091/100‑1 (MQ=255)
                                                                      acaTAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGaaaa                               <  1:585667/100‑1 (MQ=255)
                                                                      acaTAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGaaaa                               <  2:464454/100‑1 (MQ=255)
                                                                        aTAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATg                             <  1:1196845/100‑1 (MQ=255)
                                                                          aGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTTCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATgcc                           >  2:833089/1‑100 (MQ=255)
                                                                           gAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATgccg                          >  1:1672701/1‑100 (MQ=255)
                                                                                 cgcTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAgg                     >  1:2341998/1‑99 (MQ=255)
                                                                                  gcTTTTTTCTGGTTTAATACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAgg                     >  2:92556/1‑98 (MQ=255)
                                                                                     tttttCTGGTTTAACACGGCCTGAACTTCTTGTGGTAAGCGCGACCATAGTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTa                <  1:994767/100‑1 (MQ=255)
                                                                                     tttttCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTa                <  1:1671928/100‑1 (MQ=255)
                                                                                     tttttCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTa                <  1:2329981/100‑1 (MQ=255)
                                                                                       tttCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTaaa              <  2:134484/100‑1 (MQ=255)
                                                                                        ttCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAAc             <  2:286137/100‑1 (MQ=255)
                                                                                         tCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACg            <  2:1701647/100‑1 (MQ=255)
                                                                                             gTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCa        <  1:2340974/100‑1 (MQ=255)
                                                                                             gTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCa        >  1:457893/1‑100 (MQ=255)
                                                                                             gTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCa        >  2:1727466/1‑100 (MQ=255)
                                                                                             gTTTAACACGGCCTGAACTTCTTGCGGCAAACGCGACCACACTTCATATGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGGGAGGTTGTAAACGCTCa        >  2:2045707/1‑100 (MQ=255)
                                                                                                tAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCACTGTACGACAAATTCAGCAGGAAAATGCCGCCAGGTTTTAAAAACTCa        >  1:817916/1‑97 (MQ=255)
                                                                                                 aaCACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCAGCCa    >  2:16434/1‑100 (MQ=255)
                                                                                                 aaCACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCAGCCa    >  1:2393224/1‑100 (MQ=255)
                                                                                                   cacGGCCTGAACTTCTTGCGGCAAGCGCGACCACTCTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCAGCCATc  <  1:46241/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGCCATCTGCATGACGGTATTAATACGGGCCGCCAGGCCACATTCGCGGGCGATTTTCGCCGCGTTAATCACATAGAAGCGCGCTTTTTTCTGGTTTAACACGGCCTGAACTTCTTGCGGCAAGCGCGACCACACTTCATCTGCGCTGTACGGCGTGTTGAGCAGGAAAATGCCGCCAGGTTTTAAACGCTCAGCCATC  >  NC_000913/1439090‑1439288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑