Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 236,569 C→T 74.3% A168V (GCC→GTC)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913236,5690CT74.3% 173.5 / 59.5 104A168V (GCC→GTC) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base C (16/11);  new base T (38/39);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC  >  NC_000913/236472‑236668
                                                                                                 |                                                                                                   
aaaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGccc                                                                                                   <  1:139908/100‑1 (MQ=255)
 aaaTGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCa                                                                                                  >  2:1676348/1‑100 (MQ=255)
 aaaTGTTTCCCGGTAAGCGCAACAACCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCa                                                                                                  <  1:1582831/100‑1 (MQ=255)
   aTGTTTCCCGGTGAGCGCAACAGCGTTGATGCGTTATGTGCTCGCTACGAAATAGATAACAGGAGACGATCGCTTCACGGGGCATTAGTCGATGcgc                                                                                                   <  2:1102178/97‑3 (MQ=255)
     gTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATc                                                                                              >  1:1972285/1‑100 (MQ=255)
      tttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATcc                                                                                             >  2:1232365/1‑100 (MQ=255)
       ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCt                                                                                            >  2:468194/1‑100 (MQ=255)
       ttCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCt                                                                                            >  2:195826/1‑100 (MQ=255)
         cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTg                                                                                          <  1:2204517/100‑1 (MQ=255)
         cccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTg                                                                                          >  1:371147/1‑100 (MQ=255)
          ccGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGc                                                                                         <  1:1232377/100‑1 (MQ=255)
            ggTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCgg                                                                                       >  1:1037226/1‑100 (MQ=255)
             gTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCgg                                                                                       <  2:1568202/99‑1 (MQ=255)
               aaGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAg                                                                                    <  2:1193094/100‑1 (MQ=255)
                aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGTACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAgn                                                                                   <  2:1421092/100‑2 (MQ=255)
                aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGt                                                                                   <  1:320706/100‑1 (MQ=255)
                aGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGt                                                                                   <  2:732258/100‑1 (MQ=255)
                 gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGtt                                                                                  >  1:2604382/1‑100 (MQ=255)
                 gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGtt                                                                                  >  2:2224274/1‑100 (MQ=255)
                 gcgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAAAGAACGATGAACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGtt                                                                                  >  1:848282/1‑100 (MQ=255)
                  cgcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGttt                                                                                 >  1:1005830/1‑100 (MQ=255)
                   gcAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTa                                                                                >  2:2428694/1‑100 (MQ=255)
                         gCCTCGATGCGTTATGTGCTCGCTACGAAATATATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                          >  1:2523329/1‑100 (MQ=255)
                         gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                          >  2:11606/1‑100 (MQ=255)
                         gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                          >  2:1816527/1‑100 (MQ=255)
                         gCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGc                                                                          <  2:1434489/100‑1 (MQ=255)
                          ccTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCg                                                                         <  2:1153571/100‑1 (MQ=255)
                          ccTCGATGCGTTATGTGCACGCTACGAAATAGATAACAGTAAACGAACACTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCg                                                                         >  2:179934/1‑100 (MQ=255)
                           cTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGa                                                                        >  1:1376107/1‑100 (MQ=255)
                            tCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGAt                                                                       >  2:2593012/1‑100 (MQ=255)
                             cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATg                                                                      <  1:540994/100‑1 (MQ=255)
                             cGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATATCAAAATCATTGCGAAAGTATATCTGACg                                                                         >  1:141374/1‑97 (MQ=255)
                                tGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGAcc                                                                   <  2:2226692/100‑1 (MQ=255)
                                 gCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGATCGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGAcc                                                                   <  2:221026/99‑1 (MQ=255)
                                 gCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGGACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCg                                                                  <  1:258381/100‑1 (MQ=255)
                                   gTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggt                                                                <  2:1942009/100‑1 (MQ=255)
                                     tATGTGCTCGCTACGAAATAGATAACAGTAAAGGAACGCGGCGCGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                                                              <  2:1859586/100‑1 (MQ=255)
                                     tATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCggtgg                                                              <  2:2552282/100‑1 (MQ=255)
                                         tgCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATACTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCaa                                                          >  1:1860074/1‑100 (MQ=255)
                                          gctcgctcCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCCGTGGTCaaa                                                         >  2:585435/1‑100 (MQ=255)
                                           cTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAAc                                                        >  2:119728/1‑100 (MQ=255)
                                             cGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGt                                                      >  1:1331544/1‑100 (MQ=255)
                                              gCTAAGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGAACGGTGGTCAAAc                                                        >  1:1725410/1‑97 (MQ=255)
                                               cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    >  2:1508561/1‑100 (MQ=255)
                                               cTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCg                                                    >  2:164649/1‑100 (MQ=255)
                                                 aCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                                  <  1:142262/100‑1 (MQ=255)
                                                 aCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                                  <  2:2398775/100‑1 (MQ=255)
                                                 aCGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                                  <  2:1037216/100‑1 (MQ=255)
                                                 aCGAAATAGATAACACTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                                  <  1:2615959/100‑1 (MQ=255)
                                                  cGAATTAGATAACAGTAAACGAAGGCGGTACGGGTCAGTACTCGATGTCAACACCCTAGCGGAAGTTGATCTGACGGTGACCGTTGGGCAAGCGCGGATg                                                 >  2:2380698/1‑100 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGAt                                                  >  2:101505/1‑99 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                                 <  2:1613398/100‑1 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                                 <  1:572747/100‑1 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                                 <  2:381912/100‑1 (MQ=255)
                                                  cGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATg                                                 <  2:1078450/100‑1 (MQ=255)
                                                   gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                                                >  1:310045/1‑100 (MQ=255)
                                                   gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                                                >  2:480/1‑100 (MQ=255)
                                                   gAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATgg                                                >  2:2224627/1‑100 (MQ=255)
                                                    aaaTAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGc                                               <  2:2378337/100‑1 (MQ=255)
                                                       tAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCttt                                            <  1:2444130/100‑1 (MQ=255)
                                                         gaaaaCAGCAACCGAACGCTGCACGGGACATAACACGATGCCCAGATCCATGCAGAAGGTTAACTGGCGATGAACGGTGGCCAAACGTCGATGGCTTTTg                                          >  2:1882452/4‑100 (MQ=255)
                                                         gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                          <  1:141636/100‑1 (MQ=255)
                                                         gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                          >  1:1347972/1‑100 (MQ=255)
                                                         gATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                          <  1:2567986/100‑1 (MQ=255)
                                                          aTAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGc                                         <  1:451810/100‑1 (MQ=255)
                                                           tAACAGTATTCGTACGCTGCACGGGGCATTACTCGATGTTCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTg                                          <  1:2386067/98‑1 (MQ=255)
                                                           tAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg                                        >  2:227741/1‑100 (MQ=255)
                                                           tAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCg                                        <  2:495035/100‑1 (MQ=255)
                                                            aaCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGa                                       >  1:4296/1‑100 (MQ=255)
                                                             aCAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTTCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGAt                                      >  1:660411/1‑100 (MQ=255)
                                                              cAGTAAACGACCGCTGCACGGGGCATTACTCGATGTCCAGACCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     <  2:1972267/100‑1 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     >  1:1968733/1‑100 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     >  1:564232/1‑100 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     >  2:2065815/1‑100 (MQ=255)
                                                              cAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATg                                     >  2:1388509/1‑100 (MQ=255)
                                                               aGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg                                    >  2:583921/1‑100 (MQ=255)
                                                               aGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATgg                                    >  1:1001359/1‑100 (MQ=255)
                                                                    acgaacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGga                               >  1:1925719/1‑100 (MQ=255)
                                                                     cgaacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgag                              >  1:2462075/1‑100 (MQ=255)
                                                                     cgaacgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgag                              <  2:284365/100‑1 (MQ=255)
                                                                      gaacgCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgaga                             >  1:871226/1‑100 (MQ=255)
                                                                         cgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGgag                              <  2:1739695/96‑1 (MQ=255)
                                                                         cgCTGCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGac                          <  2:1506498/100‑1 (MQ=255)
                                                                          gCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGaca                         <  2:2133216/100‑1 (MQ=255)
                                                                           ccgatctgggCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGacac                        <  2:1018581/93‑1 (MQ=255)
                                                                             gCACGGGGCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACacaa                      >  1:1961374/1‑100 (MQ=255)
                                                                               aCGGGTCATTACTCGATGTCCAATTAATTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAACCGTAGATGGCTTTTGCGATGGAAGGAGAGACacaac                     <  1:1866474/99‑1 (MQ=255)
                                                                                cGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGAACGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAg                   >  2:1305002/1‑100 (MQ=255)
                                                                                  gggCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGAcacagatcgg                 >  1:1018591/1‑94 (MQ=255)
                                                                                  gggCATTACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGca                 >  1:2126208/1‑100 (MQ=255)
                                                                                     cATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGcaaca              <  1:940010/100‑1 (MQ=255)
                                                                                        tACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg           <  1:1208256/100‑1 (MQ=255)
                                                                                        tACTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAgg           <  2:2190294/100‑1 (MQ=255)
                                                                                         aCTGGATGCGTAAATCTTGGCGGAAGTATATCTGGCGATGAGATTTCGTCTAACTTTAATGGATGCTGCGATGGAAGGAGAGATACAACAGTGACAAgg           <  2:320083/99‑1 (MQ=255)
                                                                                         aCTCGATTCCCAGATACAAACGGAAGTTTATCTGGCGATAAACGGTGGTCAAACGTCGATGGATTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGt          >  2:582944/1‑100 (MQ=255)
                                                                                          cTCGATGTCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTg         <  2:310043/100‑1 (MQ=255)
                                                                                           tCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGa        <  1:1676364/100‑1 (MQ=255)
                                                                                              aTGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGc     <  1:2093576/100‑1 (MQ=255)
                                                                                                 cccAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACAGAACAGCAACAAGGTGAAGcaac  >  2:1787537/1‑100 (MQ=255)
                                                                                                 cccAGATCCTTGCGGAAGATTATCTGGCGATGACCGGAGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGGGAAGcaa   >  2:498595/1‑99 (MQ=255)
                                                                                                 |                                                                                                   
AAAATGTTTCCCGGTAAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACGCTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGTGGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCAAC  >  NC_000913/236472‑236668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: