| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,731,312 | G→A | 100% | intergenic (‑155/+288) | rrsG ← / ← clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,731,312 | 0 | G | A | 84.6% | 112.0 / 16.6 | 52 | intergenic (‑155/+288) | rrsG/clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (19/25); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01 | |||||||||||
TTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAC > NC_000913/2731229‑2731406 | cctgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgc‑‑‑‑‑TTCGCTTTTTCTCAGCGGCGCgggg > 1:231610‑M1/76‑100 (MQ=255)cctgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaagcgtattatgcacaccccgcGCCGCTTTGCTTTTTCTCAgcggcg > 2:2109012‑M1/76‑100 (MQ=255) tgagaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGcgcg > 2:951157‑M1/74‑100 (MQ=255) agaactccggcagagaaagcaaaaataaatgcttgtctctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCggg > 1:1735855‑M1/72‑100 (MQ=255) gaactccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCgggg > 1:1119106‑M1/71‑100 (MQ=255) ctccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCGGGgtgt > 1:1522183‑M1/68‑100 (MQ=255) tccggcagagaaagcaaaaataaatgcttgactctgtagtgggaaagcgtattatgcacaccccgcGCCGCTTTGCTTTTTCTCAGCGGCGCGGGGtgtg > 1:1096705‑M1/67‑100 (MQ=255) tccggcagagaaagcaaaaataaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGtgtg > 2:5430‑M1/67‑100 (MQ=255) aaaaaTCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATAc < 1:1846230/97‑1 (MQ=0) aagcaaagaaatgcttgactctgtagcgggaaggcgtattatgcacaccccgcGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCtt > 2:686098‑M1/54‑100 (MQ=255) gATTGTCTGATAAATTGTTAAAGAGCAGTGTCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCt < 1:2067870/100‑1 (MQ=9) ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt < 1:2506259/100‑1 (MQ=17) ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt < 2:577465/100‑1 (MQ=17) gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc < 1:1216727/100‑1 (MQ=25) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 2:1055460/1‑100 (MQ=38) cTGATAAATTGTTAAAGAGCAGTGCCGCTACACTTTTTCTCAGCGGCGCGGGGAGTGCATAATACGCCTTCCCGCTACAGAGACAAGCATTTCTTTGCtt > 2:2445888/1‑100 (MQ=14) tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt < 1:1152928/100‑1 (MQ=255) tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt < 2:2427847/100‑1 (MQ=255) tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt < 2:2226917/99‑1 (MQ=38) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt > 2:2520102/1‑100 (MQ=255) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt > 2:230747/1‑99 (MQ=255) aTAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc < 1:1851773/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCTGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 2:2521902/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 2:1899249/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc > 1:2067601/1‑100 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGAATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc > 1:2409165/1‑100 (MQ=255) aTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg < 2:1921165/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:122625/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:496387/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:2627545/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 1:1688598/1‑100 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 1:571844/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 1:551653/1‑100 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGACAAGCATTTCTTTGCTTTTCTCTGTTg > 1:1850822/1‑100 (MQ=255) ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga < 1:2333922/100‑1 (MQ=255) tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag < 1:1753713/100‑1 (MQ=255) tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga > 1:476650/1‑99 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga > 1:1366899/1‑100 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga > 1:1366966/1‑100 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCACGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga > 2:1626886/1‑100 (MQ=255) gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc < 2:158965/100‑1 (MQ=255) cttccgatCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa > 2:265766‑M1/9‑100 (MQ=255) cAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa < 2:2305361/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 2:2075657/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 2:2069518/100‑1 (MQ=255) gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg > 1:181104/1‑99 (MQ=255) tGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga < 2:1544230/100‑1 (MQ=255) GCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag > 2:181102‑M1/1‑100 (MQ=255) GCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag > 2:1347309‑M1/1‑100 (MQ=255) CCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACACAGTCAAACATTTCTTTGCTTTTCTATGTTGAGATTCTCAGgaga < 1:245224‑M1/100‑1 (MQ=255) GCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc > 1:1358713‑M1/1‑100 (MQ=255) GCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc < 1:1056562‑M1/100‑1 (MQ=255) | TTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAC > NC_000913/2731229‑2731406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |