BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,411,299	T→C	100%	V10A (GTA→GCA)	fis →	global DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,299	0	T	C	100.0%	384.4 / ‑6.7	113	V10A (GTA→GCA)	fis	global DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (48/63); total (49/64)

Only 100 of 113 total aligned reads displayed.

AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCA  >  NC_000913/3411202‑3411392

aaCAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCAc                                                                                             <  2:1213333/100‑1 (MQ=255)

  cAGCTGGAGGCGTTGGAGGCATTCTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAACTTCTGACGCACTg                                                                                           <  1:1802440/100‑1 (MQ=255)

  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                           <  2:894064/100‑1 (MQ=255)

  cAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTg                                                                                           <  2:893896/100‑1 (MQ=255)

    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                         >  2:1323491/1‑100 (MQ=255)

    gCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAc                                                                                         >  2:571734/1‑100 (MQ=255)

       ggAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGt                                                                                      <  1:19833/100‑1 (MQ=255)

       ggAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGt                                                                                      >  2:1946602/1‑100 (MQ=255)

        gAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGAcc                                                                                        >  1:2427392/1‑97 (MQ=255)

          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                   <  1:413846/100‑1 (MQ=255)

          ggCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTc                                                                                   <  1:1760255/100‑1 (MQ=255)

             gTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGCCCGTTTCTAc                                                                                >  2:2588556/1‑100 (MQ=255)

             gTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAc                                                                                <  1:1726540/100‑1 (MQ=255)

             gTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAc                                                                                >  1:1133038/1‑100 (MQ=255)

              ttGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTAcc                                                                               <  1:923179/100‑1 (MQ=255)

               tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                              <  1:1568057/100‑1 (MQ=255)

               tGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCg                                                                              <  1:2037473/100‑1 (MQ=255)

                ggAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGt                                                                             >  2:2506739/1‑100 (MQ=255)

                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                           <  2:2316659/100‑1 (MQ=255)

                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                           <  1:31093/100‑1 (MQ=255)

                  aGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTa                                                                           <  2:249793/100‑1 (MQ=255)

                   ggCATACTTCGAAAATTTTGCGTAAACAGAAATAAATAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGAACTGACCGTTTCTACCGTTaa                                                                          >  1:1056216/1‑100 (MQ=255)

                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                        >  1:65567/1‑100 (MQ=255)

                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAct                                                                        >  2:516398/1‑100 (MQ=255)

                     cATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAAc                                                                         <  1:1981663/99‑1 (MQ=255)

                        aCTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACtctc                                                                     <  2:2031703/100‑1 (MQ=255)

                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTATATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                    <  2:1410448/100‑1 (MQ=255)

                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                    >  2:1820845/1‑100 (MQ=255)

                         cTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                    >  2:354927/1‑100 (MQ=255)

                          ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAg                                                                   <  2:1508378/100‑1 (MQ=255)

                          ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTATGACGCACTGACCGTTTCTACCGTTAACTCTCa                                                                    >  1:258056/1‑99 (MQ=255)

                          ttCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATATTCGAACAACGCGTAAGTTCTGACGCACTGACTGTTTCTACCGTTAACTCTCAg                                                                   >  2:1680703/1‑100 (MQ=255)

                           tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                  <  2:463557/100‑1 (MQ=255)

                           tCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAgg                                                                  <  2:870845/100‑1 (MQ=255)

                            cGAAGATTTTGCGTAAACACAAATAGACAGCTGACAGAACTTTGTTCGATCAACGCGTAAATTCTGACGCACTCACCGTTTCTACCGTTAACtctc                                                                     <  2:1808377/96‑1 (MQ=255)

                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                 >  2:591354/1‑100 (MQ=255)

                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                 >  2:1058198/1‑100 (MQ=255)

                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                 >  1:1633448/1‑100 (MQ=255)

                            cGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGa                                                                 >  1:2270690/1‑100 (MQ=255)

                             gAAAATTTTGCGTAAACAAAAATAAAGAGCTGACAGAACTATGTTCGAACAACACGTAAATTCTGACGCACTTACCGATTTTACCGTTAACTCACATGAt                                                                >  2:486962/1‑100 (MQ=255)

                              aaaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACGGTTAACTCTCAGGATc                                                               >  2:1309616/1‑100 (MQ=255)

                              aaaaTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATc                                                               <  2:2013242/100‑1 (MQ=255)

                                 aTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAgg                                                            <  2:731073/100‑1 (MQ=255)

                                 aTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAgg                                                            <  2:95551/100‑1 (MQ=255)

                                 aTTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAgg                                                            <  1:1092107/100‑1 (MQ=255)

                                     tGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTaan                                                        <  1:1227480/100‑2 (MQ=255)

                                     tGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAc                                                        <  2:1122540/100‑1 (MQ=255)

                                     tGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAc                                                        <  1:1642483/100‑1 (MQ=255)

                                      gCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAcc                                                       <  1:376238/100‑1 (MQ=255)

                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                      >  1:2030243/1‑100 (MQ=255)

                                       cGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAccc                                                      >  2:1352670/1‑100 (MQ=255)

                                        gTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCa                                                     >  1:90393/1‑100 (MQ=255)

                                          aaaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaa                                                   >  2:2607024/1‑100 (MQ=255)

                                          aaaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaa                                                   >  1:2041704/1‑100 (MQ=255)

                                           aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                  >  2:210947/1‑100 (MQ=255)

                                           aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                  <  2:1133028/100‑1 (MQ=255)

                                           aaCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                  >  1:853983/1‑100 (MQ=255)

                                           aaCAGAAATAAAGAGCTGACAGAAATATGTTTGAACAACGCGTAAATTCTGACGTAGTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaa                                                  <  1:1151354/100‑1 (MQ=255)

                                            aCAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCaaaaa                                                 <  1:210556/100‑1 (MQ=255)

                                              aGAATTAAAGAGCTGACAGAATTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAcc                                               <  1:2506627/100‑1 (MQ=255)

                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                              >  2:1678348/1‑100 (MQ=255)

                                               gAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAAccc                                              >  2:680646/1‑100 (MQ=255)

                                                  aTAGAGAGGTGACAGAACTATGGTCGAACAACGCGTAAATTCTGGCGCACTGACCGTGTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCt                                            <  2:2161005/99‑1 (MQ=255)

                                                  aTAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTg                                           <  2:542107/100‑1 (MQ=255)

                                                       gagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTg                                       <  1:1615696/99‑1 (MQ=255)

                                                       gagCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGa                                      >  1:593953/1‑100 (MQ=255)

                                                        agCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCACTGCGTGAc                                     >  1:1738396/1‑100 (MQ=255)

                                                        agCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAAACAAAAAACCCCTGCGTGAc                                     <  2:258054/100‑1 (MQ=255)

                                                         gCTGACAGAACTATGTTCGAACTACGCTTAAATTCTGACGCTCTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGAc                                     <  1:203936/99‑1 (MQ=255)

                                                         gCTGACAGAACTATGTTCGAACAGATCGTAAATGATGACGCTCTGACCGTCTCCACCGTTAATTTTCAGGATCAGATTACCCAAAAACCCCTGCGt                                        <  2:2253192/96‑1 (MQ=255)

                                                            gACAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCgg                                 <  2:1175822/100‑1 (MQ=255)

                                                             aCAGAACTATGTTCGATCAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGt                                <  2:1549640/100‑1 (MQ=255)

                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                               >  2:54589/1‑100 (MQ=255)

                                                              cAGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGtt                               >  2:170924/1‑100 (MQ=255)

                                                               aGAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTa                              <  2:2285492/100‑1 (MQ=255)

                                                                gAACTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTaa                             <  2:1172894/100‑1 (MQ=255)

                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                           <  1:1373593/100‑1 (MQ=255)

                                                                  aCTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAAc                           <  2:883331/100‑1 (MQ=255)

                                                                   cTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACa                          <  2:2541934/100‑1 (MQ=255)

                                                                   cTATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACa                          >  1:2580947/1‑100 (MQ=255)

                                                                    tATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAg                         <  2:1995843/100‑1 (MQ=255)

                                                                    tATGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAg                         <  2:292276/100‑1 (MQ=255)

                                                                     aTGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACTGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAgg                        <  1:462633/100‑1 (MQ=255)

                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                       <  2:2455256/100‑1 (MQ=255)

                                                                      tGTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGc                       >  1:239998/1‑100 (MQ=255)

                                                                       gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                      >  2:1976535/1‑100 (MQ=255)

                                                                       gTTCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCa                      >  2:2094426/1‑100 (MQ=255)

                                                                         tCGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACt                    >  2:56344/1‑100 (MQ=255)

                                                                          cGAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTg                   >  2:1523671/1‑100 (MQ=255)

                                                                           gAACAACGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTga                  <  1:569557/100‑1 (MQ=255)

                                                                             acaacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACACAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaag                <  2:1367788/100‑1 (MQ=255)

                                                                              caacGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaaga               <  1:1323504/100‑1 (MQ=255)

                                                                                acGCGTAAATTCTGACGCACTTACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTgaaga               <  1:2504530/98‑1 (MQ=255)

                                                                                acGCGTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAAc             <  2:1565679/100‑1 (MQ=255)

                                                                                 cgcgTAAATTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACt            >  1:1730270/1‑100 (MQ=255)

                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGTACTGAAGAACTATTTTg      <  2:1112536/100‑1 (MQ=255)

                                                                                       aaTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTg      <  1:2506769/100‑1 (MQ=255)

                                                                                        aTTCTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGc     <  1:516402/100‑1 (MQ=255)

                                                                                           cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa  >  2:2370024/1‑100 (MQ=255)

                                                                                           cTGACGCACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa  >  1:871302/1‑100 (MQ=255)

AACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAAACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCA  >  NC_000913/3411202‑3411392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`