Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,225 T→G 16.1% intergenic (‑517/+29) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,2250TG16.1% 326.7 / 62.1 132intergenic (‑517/+29)tomB/acrBHha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (49/61);  new base G (9/12);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01

Only 100 of 139 total aligned reads displayed.

TGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCA  >  NC_000913/481126‑481324
                                                                                                   |                                                                                                   
tGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCt                                                                                                     <  2:487672/100‑1 (MQ=255)
 gAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCtt                                                                                                    <  2:330321/100‑1 (MQ=255)
 gAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCtt                                                                                                    >  2:1822494/1‑100 (MQ=255)
 gAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCtt                                                                                                    >  1:1717336/1‑100 (MQ=255)
  aaTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTa                                                                                                   <  2:1372222/100‑1 (MQ=255)
   nttttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAc                                                                                                  >  2:1345602/2‑100 (MQ=255)
    ttttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTaag                                                                                                 <  2:887364/100‑3 (MQ=255)
     tttATCCGTGGTTAATACTGGTTTTCGTATGAGATGCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgc                                                                                                <  2:1579974/100‑1 (MQ=255)
       tATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCgg                                                                                              <  2:684814/100‑1 (MQ=255)
        aTCCGTTGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGc                                                                                             <  1:2016486/100‑1 (MQ=255)
         tCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGcc                                                                                            >  2:533023/1‑100 (MQ=255)
          ccGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCt                                                                                           <  2:1395533/100‑1 (MQ=255)
            gTGGTTAATACTGGTTTTCGTATGAGATCTTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTa                                                                                         <  1:1043171/100‑1 (MQ=255)
              ggTTAATACTGGTTTTCGTATGAGATCTTGATTTGGTATTTTAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttt                                                                                       <  1:105260/100‑4 (MQ=255)
              ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                       >  1:2006233/1‑100 (MQ=255)
               gTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTg                                                                                      <  1:1567856/100‑1 (MQ=255)
                ttgaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttttt                                                                                      <  1:609360/96‑5 (MQ=255)
                ttAATACTGGTTTTCGTATGAGATCCTTAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGa                                                                                     <  2:1662005/100‑1 (MQ=255)
                 tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                    <  1:2018825/100‑1 (MQ=255)
                 tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                    <  1:1883519/100‑1 (MQ=255)
                 tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                    >  1:128751/1‑100 (MQ=255)
                 tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                    <  2:185101/100‑1 (MQ=255)
                 tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttat                                                                                    <  2:1140528/100‑7 (MQ=39)
                  aaTACTGGTTTTCGTATGAGATCCTGTGTTGTTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAtt                                                                                   <  1:378873/100‑1 (MQ=255)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                   <  1:1976441/100‑8 (MQ=38)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                   <  1:1844080/100‑8 (MQ=38)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                   <  1:2160622/100‑8 (MQ=38)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                   <  1:122859/100‑8 (MQ=38)
                  aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                   <  1:1179076/100‑8 (MQ=38)
                    tACTGGTTTTCTTTTGAGTTCTGTAGTTGGTGGTTTAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAtt                                                                                   <  1:1085839/98‑1 (MQ=255)
                     aCTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTTCTCCTTCATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                >  1:237117/1‑100 (MQ=255)
                     aCTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTTCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                <  1:1033690/100‑1 (MQ=255)
                     aCTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTacac                                                                               >  2:1916528/1‑100 (MQ=255)
                      cTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTacac                                                                               >  2:1164271/1‑100 (MQ=255)
                       tGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACg                                                                              <  2:1019391/100‑1 (MQ=255)
                         gTTTTCGTATGAGATTCTGAGTTGTTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGtt                                                                            <  1:396549/100‑1 (MQ=255)
                         gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGtt                                                                            >  2:694403/1‑100 (MQ=255)
                          ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                           <  1:1908765/100‑1 (MQ=255)
                              cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                       >  1:1425531/1‑100 (MQ=255)
                              cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                       <  2:671723/100‑1 (MQ=255)
                              cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGAGGCCTTAGTGATTACACGTTGTATc                                                                       >  1:291501/1‑100 (MQ=255)
                               gTATGAGATCCTGCGTTGGTGGTTCAATTACTCCTTAATGTTCGCAGGTTATGAATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCa                                                                      >  2:107832/1‑100 (MQ=255)
                               gTATGAGATCCTGAGTTGGTTGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCa                                                                      <  1:1146737/100‑1 (MQ=255)
                                tATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                     <  2:1181021/100‑1 (MQ=255)
                                 aTGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAat                                                                    <  2:187601/100‑1 (MQ=255)
                                  tGAGATCCTGAGTTGTTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGTCTTAGTGATTACACGTTGTATCAatg                                                                   <  2:1619348/100‑1 (MQ=255)
                                  tGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatg                                                                   <  2:1493139/100‑1 (MQ=255)
                                   gagaTCCTGAGTTGGTGGTTCAATTACTCCTTACTGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatga                                                                  >  2:1595103/1‑100 (MQ=255)
                                   gagaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatga                                                                  >  1:745258/1‑100 (MQ=255)
                                    agaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgat                                                                 >  2:205441/1‑100 (MQ=255)
                                     gaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgatg                                                                >  1:2361380/1‑100 (MQ=255)
                                      aTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAAtgatga                                                               <  1:517174/100‑1 (MQ=255)
                                        ccTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                             <  1:2163632/100‑1 (MQ=255)
                                          tGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGa                                                           >  2:1781091/1‑100 (MQ=255)
                                           gAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGAc                                                          <  1:582970/100‑1 (MQ=255)
                                            aGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACa                                                         <  1:737298/100‑1 (MQ=255)
                                             gTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAg                                                        >  2:2302759/1‑100 (MQ=255)
                                              ttGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGt                                                       <  1:1129162/100‑1 (MQ=255)
                                                  tggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTAt                                                     <  1:2522980/98‑1 (MQ=255)
                                                   ggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGc                                                  <  2:1717320/100‑1 (MQ=255)
                                                     ttCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                                <  1:2578776/100‑1 (MQ=255)
                                                       cAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgtg                                              >  2:358835/1‑100 (MQ=255)
                                                        aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                             >  1:2658724/1‑100 (MQ=255)
                                                        aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                             >  2:1336148/1‑100 (MQ=255)
                                                         aTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCt                                            <  1:1997490/100‑1 (MQ=255)
                                                           tACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCg                                          <  1:909046/100‑1 (MQ=255)
                                                              tCCTTAATGTTCGTAGGTTATGCATAAAATAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTACCAATGATGATCGACAGTATGGCTGTGCTCGata                                       <  2:1593373/100‑1 (MQ=255)
                                                              tCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGata                                       <  1:2229359/100‑1 (MQ=255)
                                                               ccTTAATGTTCGTAGATTATGCATAAAAAAGACCGCTTACGCAGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTCTGACTGTGCTCTatat                                      >  2:1727817/1‑100 (MQ=255)
                                                                  tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                   >  1:63218/1‑100 (MQ=255)
                                                                  tAAGGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                   <  2:306586/100‑1 (MQ=255)
                                                                     tGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGATCGATATCTTCa                                 >  2:2654070/1‑99 (MQ=255)
                                                                           tAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGc                          <  2:447399/100‑1 (MQ=255)
                                                                             ggTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCgg                        <  1:2107494/100‑1 (MQ=255)
                                                                               ttATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCt                      >  2:1260011/1‑100 (MQ=255)
                                                                                 aTGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaa                    >  2:1923405/1‑100 (MQ=255)
                                                                                   gCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAg                  >  1:447412/1‑100 (MQ=255)
                                                                                    cATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgc                 <  2:2625983/100‑1 (MQ=255)
                                                                                    cATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATAACTTCATTCTTGCGGCTAAAg                  >  2:2102724/1‑99 (MQ=255)
                                                                                     aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg                >  1:830505/1‑100 (MQ=255)
                                                                                     aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg                >  2:2316045/1‑100 (MQ=255)
                                                                                      tcactaaGGCCGCGTAAGCGGCCTTAGTGATTCCACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCATCATTCTTACGACTAAAgcg                >  1:2520751/6‑99 (MQ=37)
                                                                                      tAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg               >  2:851314/1‑100 (MQ=255)
                                                                                      tAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg               >  1:936594/1‑100 (MQ=255)
                                                                                       cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCACTGATGATCGAAAGTATGGCTGTGCTCGATATTTTCATTCTTGCGGCTAAAgcggc              >  1:638545/5‑100 (MQ=255)
                                                                                       cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc              >  1:2502567/5‑100 (MQ=255)
                                                                                       aaaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc              <  2:2006214/100‑1 (MQ=255)
                                                                                       aaaaaaGGCCGCTTACGCGGCCTTAGAGATTACACGTCGTATCAATGATAATCGACAGTATGGATGTTCTCGATATCTTCATTCATACGGCTaacg                  >  2:248888/1‑94 (MQ=255)
                                                                                       aaaaaaGGCCGCTGACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg               >  2:1642283/1‑99 (MQ=255)
                                                                                        actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg             >  1:2472636/4‑100 (MQ=255)
                                                                                        aaaacGGCCGCTTACGCTGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc              >  2:1429918/1‑99 (MQ=255)
                                                                                        aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg             <  2:550450/100‑1 (MQ=255)
                                                                                        aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg             >  1:1698433/1‑100 (MQ=255)
                                                                                         ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgc                 >  1:2638487/3‑95 (MQ=255)
                                                                                          aaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc           <  2:1314429/100‑1 (MQ=255)
                                                                                           aaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg          <  1:2605480/100‑1 (MQ=255)
                                                                                           aaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg          >  2:1438164/1‑100 (MQ=255)
                                                                                               ccGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac       >  1:1870299/1‑99 (MQ=255)
                                                                                                  cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc   <  1:2463065/100‑1 (MQ=255)
                                                                                                   ttACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAAccacca  <  1:1916546/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCA  >  NC_000913/481126‑481324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: