Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,228 C→A 16.1% intergenic (‑520/+26) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,2280CA16.1% 342.1 / 54.1 129intergenic (‑520/+26)tomB/acrBHha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (48/60);  new base A (10/11);  total (58/71)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

Only 100 of 131 total aligned reads displayed.

ATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACA  >  NC_000913/481129‑481326
                                                                                                   |                                                                                                  
nttttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAc                                                                                                    >  2:1345602/2‑100 (MQ=255)
     aTCCGTTGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGc                                                                                               <  1:2016486/100‑1 (MQ=255)
     aTCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGc                                                                                               <  1:2548588/100‑1 (MQ=255)
     aTCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAACGTTCGTAGATTATGCATAAAAAAGTCCGCTTACGCGGc                                                                                               <  2:1637092/100‑1 (MQ=255)
       ccGTGGTTAATATTGGTTTTCTTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCt                                                                                             <  2:644848/100‑1 (MQ=255)
       ccGTGGTTAATACTGGTTTTCGTATGAGATCTTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCt                                                                                             <  1:1083857/100‑1 (MQ=255)
       ccGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCt                                                                                             >  2:826931/1‑100 (MQ=255)
       ccGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCt                                                                                             <  2:1395533/100‑1 (MQ=255)
         gTGGTTAATACTGGTTTTCGTATGAGATCTTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTa                                                                                           <  1:1043171/100‑1 (MQ=255)
           ggTTAATACTGGTTTTCGTATGAGATCTTGATTTGGTATTTTAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttt                                                                                         <  1:105260/100‑4 (MQ=255)
           ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                         >  1:2006233/1‑100 (MQ=255)
            gTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTg                                                                                        <  1:1567856/100‑1 (MQ=255)
            gTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTg                                                                                        <  1:2006858/100‑1 (MQ=255)
            gTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTg                                                                                        <  1:1940202/100‑1 (MQ=255)
             ttgaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtttttt                                                                                        <  1:609360/96‑5 (MQ=255)
             ttAATACTGGTTTTCGTATGAGATCCTTAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGa                                                                                       <  2:1662005/100‑1 (MQ=255)
              tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                      >  1:128751/1‑100 (MQ=255)
              tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                      <  1:1883519/100‑1 (MQ=255)
              tAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttat                                                                                      <  2:1140528/100‑7 (MQ=39)
               aaTACTGGTTTTCGTATGAGATCCTGTGTTGTTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAtt                                                                                     <  1:378873/100‑1 (MQ=255)
               aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                     <  1:1976441/100‑8 (MQ=38)
               aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                     <  1:2160622/100‑8 (MQ=38)
               aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                     <  1:122859/100‑8 (MQ=38)
               aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                     <  1:1844080/100‑8 (MQ=38)
               aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                     <  2:1478973/100‑8 (MQ=38)
                  aCTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTTCTCCTTCATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                  >  1:237117/1‑100 (MQ=255)
                  aCTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTTCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                  <  1:1033690/100‑1 (MQ=255)
                  aCTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTacac                                                                                 >  2:1916528/1‑100 (MQ=255)
                   cTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTacac                                                                                 >  2:1164271/1‑100 (MQ=255)
                    tGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACg                                                                                <  2:1019391/100‑1 (MQ=255)
                      gTTTTCGTATGAGATTCTGAGTTGTTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGtt                                                                              <  1:396549/100‑1 (MQ=255)
                      gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGtt                                                                              >  2:694403/1‑100 (MQ=255)
                       ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                             <  1:1903286/100‑1 (MQ=255)
                       ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                             <  2:1798477/100‑1 (MQ=255)
                           cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                         <  2:671723/100‑1 (MQ=255)
                           cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGAGGCCTTAGTGATTACACGTTGTATc                                                                         >  1:291501/1‑100 (MQ=255)
                            gTATGAGATCCTGAGTTGGTTGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCa                                                                        <  1:1146737/100‑1 (MQ=255)
                             tATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                       <  2:1181021/100‑1 (MQ=255)
                             tATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCAGCTTACGCGGACTTAGTGATTACACGTTGTATCaa                                                                       >  1:1574469/1‑100 (MQ=255)
                              aTGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAat                                                                      <  2:187601/100‑1 (MQ=255)
                               tGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatg                                                                     <  2:1493139/100‑1 (MQ=255)
                                gagaTCCTGAGTTGGTGGTTCAATTACTCCTTACTGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatga                                                                    >  2:1595103/1‑100 (MQ=255)
                                 agaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgat                                                                   >  2:205441/1‑100 (MQ=255)
                                  gaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgatg                                                                  >  1:2361380/1‑100 (MQ=255)
                                   aTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACTCGGCCTTAGTGATTACACGTTGTATCAAtgatga                                                                 <  2:1080089/100‑1 (MQ=255)
                                   aTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAAtgatga                                                                 <  1:517174/100‑1 (MQ=255)
                                      cTGAGTTGGTGGTTCAATTACTCCTTAATGTTTGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCg                                                              <  1:663523/100‑1 (MQ=255)
                                      cTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCAGAATAAACGGCCTTAGTAATTACACGTTGTATCAATGATGATCg                                                              >  1:776625/1‑100 (MQ=255)
                                       tGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGa                                                             >  2:1781091/1‑100 (MQ=255)
                                        gAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGAc                                                            <  1:582970/100‑1 (MQ=255)
                                         aGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACa                                                           <  1:737298/100‑1 (MQ=255)
                                          gTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAg                                                          <  1:1159049/100‑1 (MQ=255)
                                          gTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAg                                                          >  2:2302759/1‑100 (MQ=255)
                                           ttGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGt                                                         <  1:1129162/100‑1 (MQ=255)
                                                 gtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCATAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGc                                                    >  2:1863129/1‑99 (MQ=255)
                                                  ttCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                                  <  1:2578776/100‑1 (MQ=255)
                                                  ttCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                                  >  2:1334635/1‑100 (MQ=255)
                                                    cAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgtg                                                >  2:358835/1‑100 (MQ=255)
                                                     aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                               >  1:2658724/1‑100 (MQ=255)
                                                         aCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGa                                           <  2:488634/100‑1 (MQ=255)
                                                           tCCTTAATGTTCGTAGGTTATGCATAAAATAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTACCAATGATGATCGACAGTATGGCTGTGCTCGata                                         <  2:1593373/100‑1 (MQ=255)
                                                           tCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGata                                         <  1:2229359/100‑1 (MQ=255)
                                                            ccTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACCCGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGatat                                        >  2:276706/1‑100 (MQ=255)
                                                            ccTTAATGTTCGTAGATTATGCATAAAAAAGACCGCTTACGCAGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTCTGACTGTGCTCTatat                                        >  2:1727817/1‑100 (MQ=255)
                                                               tAAGGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                     <  2:306586/100‑1 (MQ=255)
                                                                  tGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGATCGATATCTTCa                                   >  2:2654070/1‑99 (MQ=255)
                                                                        tAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGc                            <  2:447399/100‑1 (MQ=255)
                                                                          ggTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCgg                          >  1:963718/1‑100 (MQ=255)
                                                                          ggTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCgg                          <  1:2107494/100‑1 (MQ=255)
                                                                            ttATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCt                        >  2:1260011/1‑100 (MQ=255)
                                                                            ttATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCt                        >  2:2331080/1‑100 (MQ=255)
                                                                                gCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAg                    >  2:1584439/1‑100 (MQ=255)
                                                                                gCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAg                    >  1:447412/1‑100 (MQ=255)
                                                                                 cATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATAACTTCATTCTTGCGGCTAAAg                    >  2:2102724/1‑99 (MQ=255)
                                                                                  aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg                  >  2:2316045/1‑100 (MQ=255)
                                                                                  aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg                  >  1:830505/1‑100 (MQ=255)
                                                                                   tgactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                 >  1:2462913/6‑100 (MQ=255)
                                                                                   tcactaaGGCCGCGTAAGCGGCCTTAGTGATTCCACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCATCATTCTTACGACTAAAgcg                  >  1:2520751/6‑99 (MQ=37)
                                                                                   tAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                 >  2:851314/1‑100 (MQ=255)
                                                                                   tAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                 >  1:936594/1‑100 (MQ=255)
                                                                                    cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCACTGATGATCGAAAGTATGGCTGTGCTCGATATTTTCATTCTTGCGGCTAAAgcggc                >  1:638545/5‑100 (MQ=255)
                                                                                    cactaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc                >  1:2502567/5‑100 (MQ=255)
                                                                                    aaaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc                <  2:2006214/100‑1 (MQ=255)
                                                                                    aaaaaaGGCCGCTTACGCGGCCTTAGAGATTACACGTCGTATCAATGATAATCGACAGTATGGATGTTCTCGATATCTTCATTCATACGGCTaacg                    >  2:248888/1‑94 (MQ=255)
                                                                                    aaaaaaGGCCGCTGACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                 >  2:1642283/1‑99 (MQ=255)
                                                                                     actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg               >  1:2472636/4‑100 (MQ=255)
                                                                                     aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg               >  1:1264077/1‑100 (MQ=255)
                                                                                     aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg               >  1:1698433/1‑100 (MQ=255)
                                                                                     aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg               <  2:550450/100‑1 (MQ=255)
                                                                                      ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgc                   >  1:2638487/3‑95 (MQ=255)
                                                                                      ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTATGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg              >  1:2131474/3‑100 (MQ=255)
                                                                                      aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg              <  1:596439/100‑1 (MQ=255)
                                                                                      aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg              >  2:1374970/1‑100 (MQ=255)
                                                                                      aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcgg              <  2:1702119/100‑1 (MQ=255)
                                                                                       aaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc             <  2:1314429/100‑1 (MQ=255)
                                                                                        aaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg            <  1:2605480/100‑1 (MQ=255)
                                                                                        aaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg            >  2:1438164/1‑100 (MQ=255)
                                                                                            ccGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac         >  1:1870299/1‑99 (MQ=255)
                                                                                                ttACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAAccacca    <  1:1916546/100‑1 (MQ=255)
                                                                                                  aCGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca  <  1:825489/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
ATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACA  >  NC_000913/481129‑481326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: