Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,619 Δ2 bp pseudogene (78‑79/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,6160C.99.0% 469.6 / ‑2.0 99pseudogene (75/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (37/61);  total (38/61)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
*NC_0009133,951,6170G.99.0% 468.4 / ‑2.2 99pseudogene (76/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (37/61);  total (38/61)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 106 total aligned reads displayed.

GCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAG  >  NC_000913/3951517‑3951709
                                                                                                   ||                                                                                            
gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAc                                                                                               >  2:1020729/1‑100 (MQ=255)
   ttACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgcc                                                                                            >  2:511186/1‑99 (MQ=255)
       agcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgccgttg                                                                                        <  1:1833282/100‑6 (MQ=255)
        gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgccgttgt                                                                                       >  2:543943/1‑94 (MQ=255)
        gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgccgttgt                                                                                       >  1:2767978/1‑94 (MQ=255)
        gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgccgttgt                                                                                       >  1:1052174/1‑94 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGCACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCgttgttgtt                                                                                 <  2:1574830/100‑1 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCgttgttgtt                                                                                 <  1:2263167/100‑1 (MQ=255)
             cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTa                                                                                >  1:1986501/1‑100 (MQ=255)
              gTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTaa                                                                               >  2:2342598/1‑100 (MQ=255)
                tAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTaaaa                                                                             >  2:2621828/1‑100 (MQ=255)
                 aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaac                                                                            <  2:925071/100‑1 (MQ=255)
                 aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaac                                                                            >  1:1501184/1‑100 (MQ=255)
                 aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaac                                                                            <  2:694001/100‑1 (MQ=255)
                 aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaac                                                                            <  2:608757/100‑1 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaaca                                                                           <  1:511184/100‑1 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaaca                                                                           <  2:2634953/100‑1 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaaca                                                                           <  2:292486/100‑1 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaaca                                                                           <  2:38094/100‑1 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaaca                                                                           <  1:1045263/100‑1 (MQ=255)
                    tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaacaac                                                                         >  1:2268002/1‑100 (MQ=255)
                    tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaacaac                                                                         <  2:910552/100‑1 (MQ=255)
                    tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaacaac                                                                         >  2:2444147/1‑100 (MQ=255)
                        tGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTc                                                                     <  1:1479324/100‑1 (MQ=255)
                         gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCg                                                                    <  2:648606/100‑1 (MQ=255)
                         gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCg                                                                    <  2:1566543/100‑1 (MQ=255)
                         gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCg                                                                    <  1:1069279/100‑1 (MQ=255)
                         gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCg                                                                    <  2:897923/100‑1 (MQ=255)
                          aCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCgg                                                                   <  1:1312975/100‑1 (MQ=255)
                            tGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGTCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGAt                                                                 <  1:276773/100‑1 (MQ=255)
                            tGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGAt                                                                 <  1:1020724/100‑1 (MQ=255)
                             ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATc                                                                <  2:2645790/100‑1 (MQ=255)
                             ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATc                                                                <  2:833226/100‑1 (MQ=255)
                             ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATc                                                                >  1:1111769/1‑100 (MQ=255)
                              gcagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCg                                                               <  1:747234/100‑1 (MQ=255)
                               cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                              >  2:2707793/1‑100 (MQ=255)
                                agcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTa                                                             <  1:517881/100‑1 (MQ=255)
                                 gcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTaa                                                            >  1:975111/1‑100 (MQ=255)
                                  caACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa                                                           <  2:1174263/100‑1 (MQ=255)
                                  caACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa                                                           >  2:1777554/1‑100 (MQ=255)
                                   aaCACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAc                                                          >  1:1707022/1‑100 (MQ=255)
                                    acacTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAcc                                                         <  2:1707034/100‑1 (MQ=255)
                                       cTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGc                                                      <  1:335175/100‑1 (MQ=255)
                                        tGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                     <  1:59055/100‑1 (MQ=255)
                                          cgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGa                                                   >  2:46739/1‑100 (MQ=255)
                                          cgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGa                                                   <  2:1477397/100‑1 (MQ=255)
                                           gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGAt                                                  >  1:2120633/1‑100 (MQ=255)
                                             gcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTg                                                <  2:2413192/100‑1 (MQ=255)
                                                 cTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtc                                            <  2:1692985/100‑1 (MQ=255)
                                                  tGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                                           <  2:1230874/100‑1 (MQ=255)
                                                  tGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                                           >  1:1851798/1‑100 (MQ=255)
                                                   gCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                          >  2:1131435/1‑100 (MQ=255)
                                                    cGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGACGGATCGTAAACCTGCGGATTGCGTCGTg                                         >  1:393767/1‑100 (MQ=255)
                                                      tgatgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAGACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                                       >  1:86788/1‑100 (MQ=255)
                                                      tgatgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGTATTGCGTCGTGAc                                       <  2:1943597/100‑1 (MQ=255)
                                                       gatgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                                      <  2:1135263/100‑1 (MQ=255)
                                                        atgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                                     <  2:1535258/100‑1 (MQ=255)
                                                        atgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                                     >  2:244278/1‑100 (MQ=255)
                                                         tgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                                    <  2:402932/100‑1 (MQ=255)
                                                         tgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                                    <  2:2666738/100‑1 (MQ=255)
                                                          gaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcaca                                   >  2:95823/1‑100 (MQ=255)
                                                          gaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcaca                                   >  1:2371965/1‑100 (MQ=255)
                                                            aCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGa                                 <  1:79227/100‑1 (MQ=255)
                                                            aCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGa                                 >  1:1269983/1‑100 (MQ=255)
                                                            aCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGa                                 >  1:2236391/1‑100 (MQ=255)
                                                            aCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGa                                 >  1:599191/1‑100 (MQ=255)
                                                             cATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAt                                >  2:682994/1‑100 (MQ=255)
                                                                tCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAATCCTGCGGATTGCGTCGTGACCACAGAtgtg                             <  2:216850/100‑1 (MQ=255)
                                                                tCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgtn                             <  2:1701458/100‑2 (MQ=255)
                                                                 ccTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg                            >  2:538928/1‑100 (MQ=255)
                                                                  cTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg                           <  2:1621650/100‑1 (MQ=255)
                                                                  cTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg                           <  1:1197089/100‑1 (MQ=255)
                                                                  cTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg                           <  2:872637/100‑1 (MQ=255)
                                                                  cTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg                           >  1:2796434/1‑100 (MQ=255)
                                                                    ggCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                         <  1:244277/100‑1 (MQ=255)
                                                                    ggCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                         <  1:346441/100‑1 (MQ=255)
                                                                    ggCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                         >  1:1572862/1‑100 (MQ=255)
                                                                     gCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgca                        >  1:2530576/1‑100 (MQ=255)
                                                                      cGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcag                       <  1:543941/100‑1 (MQ=255)
                                                                       gTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagc                      <  2:2768003/100‑1 (MQ=255)
                                                                        ttACGGCCATGCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGTgcagc                      <  1:314761/99‑1 (MQ=255)
                                                                         tACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc                    >  1:1446809/1‑100 (MQ=255)
                                                                           cGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                  <  2:2120651/100‑1 (MQ=255)
                                                                           cGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                  <  1:2342580/100‑1 (MQ=255)
                                                                           cGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                  >  1:2866289/1‑100 (MQ=255)
                                                                             aCCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGAATTGCGTCGTGACCACAGATGTGGGGCAGCAc                    >  2:910027/1‑98 (MQ=255)
                                                                              ccATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAt               <  2:1632018/100‑1 (MQ=255)
                                                                              ccATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAt               <  2:2309192/100‑1 (MQ=255)
                                                                               cATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtg              >  1:2226251/1‑100 (MQ=255)
                                                                                 tCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtgtg            <  2:574172/100‑1 (MQ=255)
                                                                                  cccGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAGCCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTgg           <  2:150410/100‑1 (MQ=255)
                                                                                    cGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGc         <  2:2141154/100‑1 (MQ=255)
                                                                                    cGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGc         <  2:1242261/100‑1 (MQ=255)
                                                                                    cGGTGACGCTATCTA‑‑CGCCGATGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGc         >  1:1759891/1‑100 (MQ=255)
                                                                                      gTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTg       >  2:80544/1‑100 (MQ=255)
                                                                                        gACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgcg     >  2:2757671/1‑100 (MQ=255)
                                                                                         aCGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgcgc    <  1:2222964/100‑1 (MQ=255)
                                                                                          cGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgca   <  2:1052179/100‑1 (MQ=255)
                                                                                          cGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgca   >  2:348645/1‑100 (MQ=255)
                                                                                           gCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgcag  <  1:825762/100‑1 (MQ=255)
                                                                                                   ||                                                                                            
GCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAG  >  NC_000913/3951517‑3951709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: