BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	95.8%	428.1 / 11.9	119	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (1/4); new base C (58/56); total (59/60)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379161‑3379353

gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTGCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c                                                                                                <  2:2241563/100‑1 (MQ=255)

  acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cc                                                                                             <  1:1467759/100‑1 (MQ=255)

   atctgtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑ccc                                                                                            <  2:894808/96‑1 (MQ=255)

      cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccc                                                                                         <  2:553222/100‑1 (MQ=255)

       gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccagat                                                                                        >  1:894805/1‑96 (MQ=255)

        tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccg                                                                                       >  1:373621/1‑98 (MQ=255)

        tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccg                                                                                       >  1:370627/1‑98 (MQ=255)

          gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccgcc                                                                                     >  1:2809079/1‑96 (MQ=255)

          gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccgcc                                                                                     >  1:1108722/1‑96 (MQ=255)

          gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccgcc                                                                                     >  1:2277936/1‑96 (MQ=255)

          gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccgcc                                                                                     >  1:1310655/1‑96 (MQ=255)

          gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccgc                                                                                      >  1:822849/1‑96 (MQ=255)

           taAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑c‑cccccccgcca                                                                                    >  2:2362249/1‑95 (MQ=255)

            aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAg                                                                                    >  1:189668/1‑100 (MQ=255)

            aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAg                                                                                    >  2:1087047/1‑100 (MQ=255)

            aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAg                                                                                    >  1:1468822/1‑100 (MQ=255)

              aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAgcg                                                                                  <  2:1107478/100‑1 (MQ=255)

              aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑C‑CCCCCCGCCAgcgc                                                                                 <  2:730601/100‑1 (MQ=255)

                   aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAAc                                                                             <  2:822852/100‑1 (MQ=255)

                    ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAAcc                                                                            >  1:1492707/1‑100 (MQ=255)

                     cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCa                                                                           >  2:584963/1‑100 (MQ=255)

                       ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATa                                                                         >  2:442865/1‑100 (MQ=255)

                       ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATa                                                                         <  2:1860123/100‑1 (MQ=255)

                        cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAg                                                                        <  2:535757/100‑1 (MQ=255)

                          gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGtt                                                                      >  2:2093085/1‑100 (MQ=255)

                          gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGtn                                                                      <  2:2172382/100‑2 (MQ=255)

                            ntCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTa                                                                    >  2:2144530/2‑100 (MQ=255)

                            gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTa                                                                    <  1:1619100/100‑1 (MQ=255)

                            gTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTa                                                                    <  1:1092679/100‑1 (MQ=255)

                               tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATcc                                                                 >  1:102040/1‑100 (MQ=255)

                               tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATcc                                                                 >  2:2274856/1‑100 (MQ=255)

                               tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATcc                                                                 >  2:522514/1‑100 (MQ=255)

                               tGGTTATCGACGCCCATTGTTGCTAATGGCAGATGTTTGATTTCTACCGTCGCTGAATTTTCTC‑GTTT‑‑CACACCACGCCAGCGCCAACCATAGTTTATc                                                                  <  1:2352525/99‑1 (MQ=255)

                                ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCa                                                                >  1:55952/1‑100 (MQ=255)

                                ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCa                                                                >  1:105617/1‑100 (MQ=255)

                                   tctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCAttt                                                             <  2:838520/100‑1 (MQ=255)

                                    ctcGACGCCCATTGTTGCTAATGGGCGATGGTTGATTTCTAACGCCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  1:2390202/100‑1 (MQ=255)

                                    ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGGCAGCGCCAACCATTGTTTATCCAttt                                                             >  1:897382/1‑99 (MQ=255)

                                     tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTCCC‑CCCCCCGCCAGCGCCAACCATAGTTTATCCAttt                                                             >  2:1891597/1‑99 (MQ=255)

                                     tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           >  2:330000/1‑100 (MQ=255)

                                     tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  1:1076043/99‑1 (MQ=255)

                                      cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcg                                                          >  2:1471520/1‑100 (MQ=255)

                                           cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     <  1:1566072/100‑1 (MQ=255)

                                            ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                    <  2:687872/100‑1 (MQ=255)

                                             cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                   >  2:89993/1‑100 (MQ=255)

                                              aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  <  1:86297/100‑1 (MQ=255)

                                              aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  <  1:1030480/100‑1 (MQ=255)

                                              aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  <  2:352205/100‑1 (MQ=255)

                                                tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                <  1:1704001/100‑1 (MQ=255)

                                                 gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGGATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               >  1:1546445/1‑100 (MQ=255)

                                                 gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               <  2:243813/100‑1 (MQ=255)

                                                 gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               >  1:247077/1‑100 (MQ=255)

                                                   tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             <  1:2165592/100‑1 (MQ=255)

                                                   tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:1086762/1‑100 (MQ=255)

                                                   tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:1211558/1‑100 (MQ=255)

                                                   tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             <  2:501112/100‑1 (MQ=255)

                                                   tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  2:320688/1‑100 (MQ=255)

                                                   tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGGTTATCCATTTGCGCCCGGGTTTCATc                                             >  2:2764459/1‑100 (MQ=255)

                                                    gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            >  1:467961/1‑100 (MQ=255)

                                                    gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            >  1:2248639/1‑100 (MQ=255)

                                                     cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                           >  2:2189441/1‑100 (MQ=255)

                                                      tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          >  2:2177964/1‑100 (MQ=255)

                                                      tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          <  2:1310662/100‑1 (MQ=255)

                                                       aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGTCAGCCCCAACCATAGTTTATCCATTTGGGCCCGGGTTTCATCg                                            >  1:1041535/1‑97 (MQ=255)

                                                           gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  2:1197159/1‑100 (MQ=255)

                                                           gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:1108727/100‑1 (MQ=255)

                                                            ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                    >  1:2321884/1‑100 (MQ=255)

                                                             cGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   <  2:1033213/100‑1 (MQ=255)

                                                                tGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                <  1:1970533/100‑1 (MQ=255)

                                                                tGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                <  1:165266/100‑1 (MQ=255)

                                                                 ggTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               >  1:2201411/1‑100 (MQ=255)

                                                                  gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCGCGGGTTTCATCGTGAAGTGGCGAAAg                              >  2:144761/1‑100 (MQ=255)

                                                                  gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  2:1460378/100‑1 (MQ=255)

                                                                  gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  1:73482/100‑1 (MQ=255)

                                                                  gTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  2:2809105/100‑1 (MQ=255)

                                                                    tGATTTCTAACGTCGGTGAATTTTCTCGGTTC‑‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             <  2:467963/99‑1 (MQ=255)

                                                                    tGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                            <  1:1088065/100‑1 (MQ=255)

                                                                    tGATTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                            <  2:2248657/100‑1 (MQ=255)

                                                                      aTTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          <  1:2362231/100‑1 (MQ=255)

                                                                      aTTTCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                          >  1:1146826/1‑100 (MQ=255)

                                                                       tttCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  2:2305590/100‑1 (MQ=255)

                                                                        ttCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        >  1:2492608/1‑100 (MQ=255)

                                                                         tCTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                       <  2:205772/100‑1 (MQ=255)

                                                                          cTAACGTCGGTGAATTTTCTC‑GTTTCCC‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCTCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                       <  1:1891583/100‑1 (MQ=255)

                                                                          cTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                      >  1:2768/1‑100 (MQ=255)

                                                                          cTAACGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                      <  2:1137962/100‑1 (MQ=255)

                                                                            aaCGTCGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                    <  2:1134223/100‑1 (MQ=255)

                                                                               gccGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 <  2:1492719/98‑1 (MQ=255)

                                                                                 cGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  2:247078/100‑1 (MQ=255)

                                                                                 cGGTGAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg               <  1:772476/100‑1 (MQ=255)

                                                                                     gAATTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  1:2884917/100‑1 (MQ=255)

                                                                                       tccatctc‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         <  1:2283122/96‑1 (MQ=255)

                                                                                       aTTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:2765685/1‑100 (MQ=255)

                                                                                       aTTTTCTC‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag         >  2:1584083/1‑100 (MQ=255)

                                                                                       aTTTTCTC‑GTTTC‑C‑CCCCCCCCCCGCCCCCACCATATTTTATTCCTTTGCGCCCGGGGTTCCACGGTAAGGGGGGAAAGCCACCGAAGCGcc                 >  1:508756/1‑92 (MQ=255)

                                                                                           tctc‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa     >  2:685969/1‑100 (MQ=255)

                                                                                            ctc‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTAc    >  1:1311956/1‑100 (MQ=255)

                                                                                              c‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgagagatcgg  >  2:2283140/1‑93 (MQ=255)

                                                                                              c‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  2:1340158/100‑1 (MQ=255)

                                                                                              c‑GTTTC‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  >  2:1930844/1‑100 (MQ=255)

GCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTC‑GTTT‑‑C‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379161‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`