Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,916,004 (T)6→7 coding (1372/1383 nt) atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,915,9981.T95.5% 403.8 / 10.2 111coding (1378/1383 nt)atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base T (53/53);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 128 total aligned reads displayed.

TGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGCCTTT  >  NC_000913/3915899‑3916091
                                                                                                    |                                                                                             
tGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAg                                                                                                <  1:525497/100‑1 (MQ=255)
tGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAg                                                                                                >  2:1463936/1‑100 (MQ=255)
tGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAg                                                                                                <  1:1537466/100‑1 (MQ=255)
   aTTTTCTCGACCCGACCAGACAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCGGATTAAGCCGTTAAAg                                                                                                >  1:558138/1‑97 (MQ=255)
   aTTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGttt                                                                                            <  1:1211301/100‑1 (MQ=255)
     tttCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGttttt                                                                                          <  2:496437/100‑1 (MQ=255)
        ctcGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttg                                                                                       <  1:481905/100‑3 (MQ=255)
        ctcGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttg                                                                                       <  2:2305348/100‑3 (MQ=255)
        ctcGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttg                                                                                       <  1:673674/100‑3 (MQ=255)
        ctcGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttg                                                                                       >  2:2772280/1‑98 (MQ=255)
        ctcGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttg                                                                                       >  2:552843/1‑98 (MQ=255)
        ctcGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGACGTTAAAGtttttttg                                                                                       >  1:1689297/1‑98 (MQ=255)
          cGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttggc                                                                                     <  2:1316533/100‑5 (MQ=255)
           gaccagaccaGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGtttttttggct                                                                                    >  2:375907/1‑95 (MQ=255)
            accagaccagAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTCAAGTTTTTTTGGCtt                                                                                    <  1:1868884/100‑1 (MQ=255)
            accagaccagAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCtt                                                                                    <  1:745026/100‑1 (MQ=255)
             ccagaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCttt                                                                                   >  2:2121842/1‑100 (MQ=255)
             ccagaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCttt                                                                                   <  1:2294235/100‑1 (MQ=255)
              cagaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCtttt                                                                                  <  2:2759801/100‑1 (MQ=255)
              cagaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCtttt                                                                                  <  1:1541598/100‑1 (MQ=255)
              cagaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCtttt                                                                                  <  1:1091287/100‑1 (MQ=255)
               agaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCttttt                                                                                 <  2:2375994/100‑1 (MQ=255)
               agaccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCttttt                                                                                 >  1:1884648/1‑100 (MQ=255)
                  ccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCca                                                                              >  1:1952605/1‑100 (MQ=255)
                  ccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCca                                                                              >  2:2143234/1‑100 (MQ=255)
                  ccagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCca                                                                              >  2:1659017/1‑100 (MQ=255)
                   cagaGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCcac                                                                             <  2:1212873/100‑1 (MQ=255)
                    agagAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCcaca                                                                            <  1:2635943/100‑1 (MQ=255)
                      agaACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGc                                                                          >  1:83209/1‑100 (MQ=255)
                       gaACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGct                                                                         >  1:274434/1‑100 (MQ=255)
                          cATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGATTTTTCCACAGcttct                                                                      >  1:1693016/1‑100 (MQ=255)
                             ttgttgCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCg                                                                   <  2:606358/100‑1 (MQ=255)
                              tgttgCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGa                                                                  <  1:1585670/100‑1 (MQ=255)
                               gttgCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGAt                                                                 <  1:1370581/100‑1 (MQ=255)
                               gttgCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGAt                                                                 <  2:764374/100‑1 (MQ=255)
                                ttgCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATg                                                                <  1:311765/100‑1 (MQ=255)
                                ttgCTCTGCGCTGACGACGTCCAGGTGGTAAGTCAGTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATg                                                                <  1:272168/100‑1 (MQ=255)
                                  gCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGa                                                              >  1:241610/1‑100 (MQ=255)
                                  gCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGa                                                              >  1:2699208/1‑100 (MQ=255)
                                   ctctGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGaa                                                             >  2:1387740/1‑100 (MQ=255)
                                   ctctGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGaa                                                             >  2:1494943/1‑100 (MQ=255)
                                   ctctGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGaa                                                             >  1:2298198/1‑100 (MQ=255)
                                    tctGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAAc                                                            >  1:2844118/1‑100 (MQ=255)
                                        cgcTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGAc                                                        >  1:2840347/1‑100 (MQ=255)
                                        cgcTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGAc                                                        >  2:2056777/1‑100 (MQ=255)
                                         gcTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGAcc                                                       >  2:1934976/1‑100 (MQ=255)
                                         gcTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGAcc                                                       <  2:1590915/100‑1 (MQ=255)
                                          cTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCa                                                      <  2:1689309/100‑1 (MQ=255)
                                           tGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCAt                                                     <  2:274435/100‑1 (MQ=255)
                                            gacgacGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATg                                                    >  2:676208/1‑100 (MQ=255)
                                             acgacgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGt                                                   <  1:2009884/100‑1 (MQ=255)
                                              cgacgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTa                                                  <  1:323341/100‑1 (MQ=255)
                                              cgacgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTa                                                  <  1:2551857/100‑1 (MQ=255)
                                              cgacgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTa                                                  >  1:1328939/1‑100 (MQ=255)
                                              cgacgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTa                                                  >  1:2177007/1‑100 (MQ=255)
                                                acgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACATCTTCTTCGATGGAACCGACCATGTAGa                                                >  1:1005441/1‑100 (MQ=255)
                                                acgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGa                                                <  2:2177025/100‑1 (MQ=255)
                                                 cgTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGaa                                               >  1:977691/1‑100 (MQ=255)
                                                   tCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCTACACCTTCTTCCATGGAACCGCCCATGTATAACg                                             >  1:1600927/1‑100 (MQ=255)
                                                   tCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACg                                             >  2:2704353/1‑100 (MQ=255)
                                                   tCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACg                                             >  2:910761/1‑100 (MQ=255)
                                                   tCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGc                                            <  1:336919/100‑1 (MQ=255)
                                                       ggtggtAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTg                                         >  1:2479558/1‑100 (MQ=255)
                                                        gtggtAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGc                                        <  1:639825/100‑1 (MQ=255)
                                                          ggtAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTc                                      >  1:84166/1‑100 (MQ=255)
                                                           gtAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAGAGTTTTTTTGGCTTTTTCCTCAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTcc                                     <  1:1579450/100‑1 (MQ=255)
                                                           gtAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTcc                                     <  1:71917/100‑1 (MQ=255)
                                                            tAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCg                                    >  1:930199/1‑100 (MQ=255)
                                                            tAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCg                                    <  1:111185/100‑1 (MQ=255)
                                                              aGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGc                                  <  1:107642/100‑1 (MQ=255)
                                                               gTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCa                                 >  1:2846593/1‑100 (MQ=255)
                                                                tCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAg                                <  1:2871733/100‑1 (MQ=255)
                                                                  aTTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGt                              <  2:541886/100‑1 (MQ=255)
                                                                  aTTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGt                              >  2:2120527/1‑100 (MQ=255)
                                                                    tGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGa                            <  1:827934/100‑1 (MQ=255)
                                                                     gCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGAt                           >  1:1264705/1‑100 (MQ=255)
                                                                      ccATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATc                          >  1:828809/1‑100 (MQ=255)
                                                                       cATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCg                         <  1:1659005/100‑1 (MQ=255)
                                                                       cATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCg                         <  2:2632086/100‑1 (MQ=255)
                                                                        atatCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGt                        >  1:2319043/1‑100 (MQ=255)
                                                                          atCACCCTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTAt                      >  2:939841/1‑100 (MQ=255)
                                                                              cccTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGc                  >  1:2757618/1‑100 (MQ=255)
                                                                              cccTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGc                  >  1:2527843/1‑100 (MQ=255)
                                                                              cccTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGc                  <  2:1643307/100‑1 (MQ=255)
                                                                                cTCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCt                >  1:914325/1‑100 (MQ=255)
                                                                                 tCCGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCtt               <  2:1884662/100‑1 (MQ=255)
                                                                                 tCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTc              >  2:2669070/1‑100 (MQ=255)
                                                                                   cGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTcc             <  1:2143216/100‑1 (MQ=255)
                                                                                   cGATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTcc             >  2:665254/1‑100 (MQ=255)
                                                                                    gATTAAGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCa            <  1:2121824/100‑1 (MQ=255)
                                                                                    ccgatctgCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCa            <  1:1589843/93‑1 (MQ=255)
                                                                                     aTTAAGGCGTTAAAGTTTTTTTGGCTTTTTTAACCGATTCTTTGATGGCCAGGTCCATGTCGCTCGACTGTTCCGGCAGGTGATCGTATTCGCCTTCCat           <  1:1682189/100‑1 (MQ=255)
                                                                                         aGGCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCatgatg       >  1:42572/1‑100 (MQ=255)
                                                                                         aGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGc      >  2:2050870/1‑100 (MQ=255)
                                                                                          ggCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGc      <  2:1104176/100‑1 (MQ=255)
                                                                                           gCGTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCaagatcgg     >  2:1589855/1‑97 (MQ=255)
                                                                                             gTTAAAGTTTTTTTGTTTTTTTTCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATACCtt   >  1:2648370/1‑100 (MQ=255)
                                                                                             gTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGCCtt   <  2:83209/100‑1 (MQ=255)
                                                                                             gTTAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGCCtt   <  2:1153311/100‑1 (MQ=255)
                                                                                              ttAAAGTTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGCCttt  <  1:230461/100‑1 (MQ=255)
                                                                                                    |                                                                                             
TGGATTTTCTCGACCAGACCAGAGAACATTTGTTGCTCTGCGCTGACGACGTCCAGGTGGTAAGTCATTGCCATATCACCCTCCGATTAAGGCGTTAAAGTTTTTTGGCTTTTTCCACAGCTTCTTCGATGGAACCGACCATGTAGAACGCCTGCTCCGGCAGGTGATCGTATTCGCCTTCCATGATGCCTTT  >  NC_000913/3915899‑3916091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: